4eif - Revision history

OCA at 09:58, 30 October 2024

2024-10-30T09:58:01Z

← Older revision		Revision as of 09:58, 30 October 2024
Line 9:		Line 9:
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~Q8KX15_SYNP2 Q8KX15_SYNP2~~]		[https://www.uniprot.org/uniprot/Q8KX15_PICP2 Q8KX15_PICP2]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The cyanobacterium Synechococcus sp. PCC 7002 carries two genes, petJ1 and petJ2, for proteins related to soluble, cytochrome c6 electron transfer proteins. PetJ1 was purified from the cyanobacterium, and both cytochromes were expressed with heme incorporation in Escherichia coli. The expressed PetJ1 displayed spectral and biochemical properties virtually identical to those of PetJ1 from Synechococcus. PetJ1 is a typical cytochrome c6 but contains an unusual KDGSKSL insertion. PetJ2 isolated from E. coli exhibited absorbance spectra characteristic of cytochromes, although the alpha, beta, and gamma bands were red-shifted relative to those of PetJ1. Moreover, the surface electrostatic properties and redox midpoint potential of PetJ2 (pI 9.7; E(m,7) = 148 +/- 1.7 mV) differed substantially from those of PetJ1 (pI 3.8; E(m,7) = 319 +/- 1.6 mV). These data indicate that the PetJ2 cytochrome could not effectively replace PetJ1 as an electron acceptor for the cytochrome bf complex in photosynthesis. Phylogenetic comparisons against plant, algal, bacterial, and cyanobacterial genomes revealed two novel and widely distributed clusters of previously uncharacterized, cyanobacterial c 6-like cytochromes. PetJ2 belongs to a group that is distinct from both c6 cytochromes and the enigmatic chloroplast c 6A cytochromes. We tentatively designate the PetJ2 group as c6C cytochromes and the other new group as c6B cytochromes. Possible functions of these cytochromes are discussed.

			Deeply branching c6-like cytochromes of cyanobacteria.,Bialek W, Nelson M, Tamiola K, Kallas T, Szczepaniak A Biochemistry. 2008 May 20;47(20):5515-22. Epub 2008 Apr 26. PMID:018439023<ref>PMID:018439023</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4eif" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:02, 14 March 2024

2024-03-14T15:02:17Z

← Older revision		Revision as of 15:02, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_sp._PCC_7002 Synechococcus sp. PCC 7002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_sp._PCC_7002 Synechococcus sp. PCC 7002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.04Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [https://pdbe.org/4eif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [https://www.ebi.ac.uk/pdbsum/4eif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eif ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [https://pdbe.org/4eif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [https://www.ebi.ac.uk/pdbsum/4eif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eif ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:40, 14 December 2022

2022-12-14T10:40:30Z

← Older revision		Revision as of 10:40, 14 December 2022
Line 3:		Line 3:
	<StructureSection load='4eif' size='340' side='right'caption='[[4eif]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='4eif' size='340' side='right'caption='[[4eif]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Agmenellum_quadruplicatum Agmenellum quadruplicatum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_sp._PCC_7002 Synechococcus sp. PCC 7002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3dr0\|3dr0]], [[4eic\|4eic]], [[4eid\|4eid]], [[4eie\|4eie]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">petJ, petJ2, SYNPCC7002_A2391 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32049 Agmenellum quadruplicatum])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [https://pdbe.org/4eif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [https://www.ebi.ac.uk/pdbsum/4eif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eif ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [https://pdbe.org/4eif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [https://www.ebi.ac.uk/pdbsum/4eif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eif ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q8KX15_SYNP2 Q8KX15_SYNP2]
	The cyanobacterium Synechococcus sp. PCC 7002 carries two genes, petJ1 and petJ2, for proteins related to soluble, cytochrome c6 electron transfer proteins. PetJ1 was purified from the cyanobacterium, and both cytochromes were expressed with heme incorporation in Escherichia coli. The expressed PetJ1 displayed spectral and biochemical properties virtually identical to those of PetJ1 from Synechococcus. PetJ1 is a typical cytochrome c6 but contains an unusual KDGSKSL insertion. PetJ2 isolated from E. coli exhibited absorbance spectra characteristic of cytochromes, although the alpha, beta, and gamma bands were red-shifted relative to those of PetJ1. Moreover, the surface electrostatic properties and redox midpoint potential of PetJ2 (pI 9.7; E(m,7) = 148 +/- 1.7 mV) differed substantially from those of PetJ1 (pI 3.8; E(m,7) = 319 +/- 1.6 mV). These data indicate that the PetJ2 cytochrome could not effectively replace PetJ1 as an electron acceptor for the cytochrome bf complex in photosynthesis. Phylogenetic comparisons against plant, algal, bacterial, and cyanobacterial genomes revealed two novel and widely distributed clusters of previously uncharacterized, cyanobacterial c 6-like cytochromes. PetJ2 belongs to a group that is distinct from both c6 cytochromes and the enigmatic chloroplast c 6A cytochromes. We tentatively designate the PetJ2 group as c6C cytochromes and the other new group as c6B cytochromes. Possible functions of these cytochromes are discussed.

	~~Deeply branching c6-like cytochromes of cyanobacteria.,Bialek W, Nelson M, Tamiola K, Kallas T, Szczepaniak A Biochemistry. 2008 May 20;47(20):5515-22. Epub 2008 Apr 26. PMID:18439023<ref>PMID~~:~~18439023<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 4eif" style="background-color:#fffaf0;"><~~/~~div>~~

	~~==See Also==~~
	*[[Cytochrome C 3D structures\|Cytochrome C 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Agmenellum quadruplicatum]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bialek, W]]		[[Category: Synechococcus sp. PCC 7002]]
	[[Category: Jaskolski, M]]		[[Category: Bialek W]]
	[[Category: Krzywda, S]]		[[Category: Jaskolski M]]
	[[Category: Szczepaniak, A]]		[[Category: Krzywda S]]
	[[Category: Zatwarnicki, P]]		[[Category: Szczepaniak A]]
	~~[[Category: Cytochrome c6c]]~~		[[Category: Zatwarnicki P]]
	~~[[Category: Electron transport~~]]

OCA at 17:27, 10 March 2021

2021-03-10T17:27:51Z

← Older revision		Revision as of 17:27, 10 March 2021
Line 1:		Line 1:

	==Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002==		==Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002==
	<StructureSection load='4eif' size='340' side='right' caption='[[4eif]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='4eif' size='340' side='right'caption='[[4eif]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Agmenellum_quadruplicatum Agmenellum quadruplicatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EIF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agmenellum_quadruplicatum Agmenellum quadruplicatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=~~HEM~~:~~PROTOPORPHYRIN~~+~~IX+CONTAINING+FE~~'>~~HEM~~</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dr0\|3dr0]], [[4eic\|4eic]], [[4eid\|4eid]], [[4eie\|4eie]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3dr0\|3dr0]], [[4eic\|4eic]], [[4eid\|4eid]], [[4eie\|4eie]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">petJ, petJ2, SYNPCC7002_A2391 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32049 Agmenellum quadruplicatum])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">petJ, petJ2, SYNPCC7002_A2391 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32049 Agmenellum quadruplicatum])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [~~http~~://pdbe.org/4eif PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eif PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eif ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [https://pdbe.org/4eif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [https://www.ebi.ac.uk/pdbsum/4eif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eif ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
	*[[Cytochrome c\|Cytochrome c]]		*[[Cytochrome C 3D structures\|Cytochrome C 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Agmenellum quadruplicatum]]		[[Category: Agmenellum quadruplicatum]]
			[[Category: Large Structures]]
	[[Category: Bialek, W]]		[[Category: Bialek, W]]
	[[Category: Jaskolski, M]]		[[Category: Jaskolski, M]]

OCA at 18:49, 5 August 2016

2016-08-05T18:49:49Z

← Older revision		Revision as of 18:49, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002==		==Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002==
	<StructureSection load='4eif' size='340' side='right' caption='[[4eif]], [[Resolution\|resolution]] 1.04Å' scene=''>		<StructureSection load='4eif' size='340' side='right' caption='[[4eif]], [[Resolution\|resolution]] 1.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Synechococcus_sp._pcc_7002 Synechococcus sp. pcc 7002~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agmenellum_quadruplicatum Agmenellum quadruplicatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dr0\|3dr0]], [[4eic\|4eic]], [[4eid\|4eid]], [[4eie\|4eie]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dr0\|3dr0]], [[4eic\|4eic]], [[4eid\|4eid]], [[4eie\|4eie]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">petJ, petJ2, SYNPCC7002_A2391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32049 ~~Synechococcus sp. PCC 7002~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">petJ, petJ2, SYNPCC7002_A2391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32049 Agmenellum quadruplicatum])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [http://www.ebi.ac.uk/pdbsum/4eif PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [http://pdbe.org/4eif PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [http://www.ebi.ac.uk/pdbsum/4eif PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eif ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eif" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Synechococcus sp. pcc 7002~~]]		[[Category: Agmenellum quadruplicatum]]
	[[Category: Bialek, W]]		[[Category: Bialek, W]]
	[[Category: Jaskolski, M]]		[[Category: Jaskolski, M]]

OCA at 11:17, 21 December 2014

2014-12-21T11:17:44Z

← Older revision		Revision as of 11:17, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4eif\| PDB~~=4eif \| ~~SCENE~~= }}		==Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002==
	===~~Crystal~~ structure ~~of cytochrome c6C L50Q mutant~~ from Synechococcus sp. PCC 7002===		<StructureSection load='4eif' size='340' side='right' caption='[[4eif]], [[Resolution\|resolution]] 1.04Å' scene=''>
	~~{{ABSTRACT_PUBMED_18439023}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4eif]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp._pcc_7002 Synechococcus sp. pcc 7002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIF FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3dr0\|3dr0]], [[4eic\|4eic]], [[4eid\|4eid]], [[4eie\|4eie]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">petJ, petJ2, SYNPCC7002_A2391 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32049 Synechococcus sp. PCC 7002])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eif OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eif RCSB], [http://www.ebi.ac.uk/pdbsum/4eif PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The cyanobacterium Synechococcus sp. PCC 7002 carries two genes, petJ1 and petJ2, for proteins related to soluble, cytochrome c6 electron transfer proteins. PetJ1 was purified from the cyanobacterium, and both cytochromes were expressed with heme incorporation in Escherichia coli. The expressed PetJ1 displayed spectral and biochemical properties virtually identical to those of PetJ1 from Synechococcus. PetJ1 is a typical cytochrome c6 but contains an unusual KDGSKSL insertion. PetJ2 isolated from E. coli exhibited absorbance spectra characteristic of cytochromes, although the alpha, beta, and gamma bands were red-shifted relative to those of PetJ1. Moreover, the surface electrostatic properties and redox midpoint potential of PetJ2 (pI 9.7; E(m,7) = 148 +/- 1.7 mV) differed substantially from those of PetJ1 (pI 3.8; E(m,7) = 319 +/- 1.6 mV). These data indicate that the PetJ2 cytochrome could not effectively replace PetJ1 as an electron acceptor for the cytochrome bf complex in photosynthesis. Phylogenetic comparisons against plant, algal, bacterial, and cyanobacterial genomes revealed two novel and widely distributed clusters of previously uncharacterized, cyanobacterial c 6-like cytochromes. PetJ2 belongs to a group that is distinct from both c6 cytochromes and the enigmatic chloroplast c 6A cytochromes. We tentatively designate the PetJ2 group as c6C cytochromes and the other new group as c6B cytochromes. Possible functions of these cytochromes are discussed.

	~~==About this Structure==~~		Deeply branching c6-like cytochromes of cyanobacteria.,Bialek W, Nelson M, Tamiola K, Kallas T, Szczepaniak A Biochemistry. 2008 May 20;47(20):5515-22. Epub 2008 Apr 26. PMID:18439023<ref>PMID:18439023</ref>
	~~[[4eif]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Synechococcus_sp~~.~~_pcc_7002 Synechococcus sp~~. ~~pcc 7002]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group~~=~~"xtra">PMID:018439023</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>

			==See Also==
			*[[Cytochrome c\|Cytochrome c]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Synechococcus sp. pcc 7002]]		[[Category: Synechococcus sp. pcc 7002]]
	[[Category: Bialek, W.]]		[[Category: Bialek, W]]
	[[Category: Jaskolski, M.]]		[[Category: Jaskolski, M]]
	[[Category: Krzywda, S.]]		[[Category: Krzywda, S]]
	[[Category: Szczepaniak, A.]]		[[Category: Szczepaniak, A]]
	[[Category: Zatwarnicki, P.]]		[[Category: Zatwarnicki, P]]
	[[Category: Cytochrome c6c]]		[[Category: Cytochrome c6c]]
	[[Category: Electron transport]]		[[Category: Electron transport]]

OCA at 22:40, 10 April 2013

2013-04-10T22:40:47Z

← Older revision		Revision as of 22:40, 10 April 2013
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	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_4eif\| PDB=4eif \| SCENE= }}
			===Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002===
			{{ABSTRACT_PUBMED_18439023}}

	~~The entry~~ 4eif is ~~ON HOLD until Apr 10 2014~~		==About this Structure==
			[[4eif]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp._pcc_7002 Synechococcus sp. pcc 7002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIF OCA].

	~~Authors~~: ~~Krzywda, S~~., Bialek, W.~~, Zatwarnicki, P.,~~ Jaskolski, M., Szczepaniak, A.		==Reference==
			<ref group="xtra">PMID:018439023</ref><references group="xtra"/><references/>
	~~Description~~: ~~Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002~~		[[Category: Synechococcus sp. pcc 7002]]
			[[Category: Bialek, W.]]
			[[Category: Jaskolski, M.]]
			[[Category: Krzywda, S.]]
			[[Category: Szczepaniak, A.]]
			[[Category: Zatwarnicki, P.]]
			[[Category: Cytochrome c6c]]
			[[Category: Electron transport]]

OCA at 16:07, 20 March 2013

2013-03-20T16:07:04Z

← Older revision		Revision as of 16:07, 20 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eif is ON HOLD until ~~Paper Publication~~		The entry 4eif is ON HOLD until Apr 10 2014

	Authors: Krzywda, S., Bialek, W., Zatwarnicki, P., Jaskolski, M., Szczepaniak, A.		Authors: Krzywda, S., Bialek, W., Zatwarnicki, P., Jaskolski, M., Szczepaniak, A.

	Description: Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002		Description: Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002

OCA at 08:47, 16 May 2012

2012-05-16T08:47:52Z

← Older revision		Revision as of 08:47, 16 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eif is ON HOLD		The entry 4eif is ON HOLD until Paper Publication

	Authors: Krzywda, S., Bialek, W., Zatwarnicki, P., Jaskolski, M., Szczepaniak, A.		Authors: Krzywda, S., Bialek, W., Zatwarnicki, P., Jaskolski, M., Szczepaniak, A.

	Description: Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002		Description: Crystal structure of cytochrome c6C L50Q mutant from Synechococcus sp. PCC 7002

OCA: Protected "4eif" [edit=sysop:move=sysop]

2012-04-11T06:44:15Z

Protected "4eif" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:44, 11 April 2012
(No difference)