4eij - Revision history

OCA at 11:03, 1 March 2024

2024-03-01T11:03:57Z

← Older revision		Revision as of 11:03, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eij]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mumps_orthorubulavirus Mumps orthorubulavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eij]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mumps_orthorubulavirus Mumps orthorubulavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2001Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eij OCA], [https://pdbe.org/4eij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eij RCSB], [https://www.ebi.ac.uk/pdbsum/4eij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eij ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eij OCA], [https://pdbe.org/4eij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eij RCSB], [https://www.ebi.ac.uk/pdbsum/4eij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eij ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q8QY72_9MONO Q8QY72_9MONO]] Essential component of the RNA polymerase and the nascent chain assembly complex. Also required during RNA synthesis.[ARBA:ARBA00002047]		[https://www.uniprot.org/uniprot/Q8QY72_9MONO Q8QY72_9MONO] Essential component of the RNA polymerase and the nascent chain assembly complex. Also required during RNA synthesis.[ARBA:ARBA00002047]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The phosphoprotein (P) is virally encoded by the Rhabdoviridae and Paramyxoviridae in the order Mononegavirales. P is a self-associated oligomer and forms complexes with the large viral polymerase protein (L), the nucleocapsid protein (N), and the assembled nucleocapsid. P from different viruses has shown structural diversities even though their essential functions are the same. We systematically mapped the domains in mumps virus (MuV) P and investigated their interactions with nucleocapsid-like particles (NLPs). Similar to other P proteins, MuV P contains N-terminal, central, and C-terminal domains with flexible linkers between neighboring domains. By pulldown assays, we discovered that in addition to the previously proposed nucleocapsid binding domain (residues 343-391), the N-terminal region of MuV P (residues 1-194) could also bind NLP. Further analysis of binding kinetics was conducted using surface plasmon resonance. This is the first observation that both the N- and C-terminal regions of a negative strand RNA virus P are involved in binding the nucleocapsid. Additionally, we defined the oligomerization domain (POD) of MuV P as residues 213-277 and determined its crystal structure. The tetrameric MuV POD is formed by one pair of long parallel alpha-helices with another pair in opposite orientation. Unlike the parallel orientation of each alpha-helix in the tetramer of Sendai virus POD, this represents a novel orientation of a POD where both the N- and C-terminal domains are at either end of the tetramer. This is consistent with the observation that both the N- and C-terminal domains are involved in binding the nucleocapsid.

	Structural and Functional Characterization of the Mumps Virus Phosphoprotein.,Cox R, Green TJ, Purushotham S, Deivanayagam C, Bedwell GJ, Prevelige PE, Luo M J Virol. 2013 May 1. PMID:23637399<ref>PMID:23637399</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4eij" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:12, 28 September 2022

2022-09-28T07:12:52Z

← Older revision		Revision as of 07:12, 28 September 2022
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	==Structure of the Mumps virus phosphoprotein oligomerization domain==		==Structure of the Mumps virus phosphoprotein oligomerization domain==
	<StructureSection load='4eij' size='340' side='right' caption='[[4eij]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4eij' size='340' side='right'caption='[[4eij]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eij]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mumps_virus~~ Mumps ~~virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EIJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eij]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mumps_orthorubulavirus Mumps orthorubulavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11161 Mumps virus])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eij OCA], [https://pdbe.org/4eij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eij RCSB], [https://www.ebi.ac.uk/pdbsum/4eij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eij ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eij OCA], [~~http~~://pdbe.org/4eij PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eij RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eij PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eij ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/Q8QY72_9MONO Q8QY72_9MONO]] Essential component of the RNA polymerase and the nascent chain assembly complex. Also required during RNA synthesis.[ARBA:ARBA00002047]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mumps virus~~]]		[[Category: Large Structures]]
	[[Category: ~~Cox, R~~]]		[[Category: Mumps orthorubulavirus]]
	[[Category: ~~Green, T J~~]]		[[Category: Cox R]]
	[[Category: ~~Luo, M~~]]		[[Category: Green TJ]]
	[[Category: ~~Oligomerization]]~~		[[Category: Luo M]]
	~~[[Category: Replication~~]]

OCA at 14:04, 5 August 2016

2016-08-05T14:04:38Z

← Older revision		Revision as of 14:04, 5 August 2016
Line 1:		Line 1:

	==Structure of the Mumps virus phosphoprotein oligomerization domain==		==Structure of the Mumps virus phosphoprotein oligomerization domain==
	<StructureSection load='4eij' size='340' side='right' caption='[[4eij]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4eij' size='340' side='right' caption='[[4eij]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11161 Mumps virus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11161 Mumps virus])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eij RCSB], [http://www.ebi.ac.uk/pdbsum/4eij PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eij OCA], [http://pdbe.org/4eij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eij RCSB], [http://www.ebi.ac.uk/pdbsum/4eij PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eij ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eij" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:43, 21 December 2014

2014-12-21T11:43:49Z

← Older revision		Revision as of 11:43, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4eij~~\| ~~PDB~~=4eij \| ~~SCENE~~= }}		==Structure of the Mumps virus phosphoprotein oligomerization domain==
	===~~Structure of the~~ Mumps virus ~~phosphoprotein oligomerization domain~~===		<StructureSection load='4eij' size='340' side='right' caption='[[4eij]], [[Resolution\|resolution]] 2.20Å' scene=''>
	~~{{ABSTRACT_PUBMED_23637399}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4eij]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mumps_virus Mumps virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIJ FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11161 Mumps virus])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eij RCSB], [http://www.ebi.ac.uk/pdbsum/4eij PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The phosphoprotein (P) is virally encoded by the Rhabdoviridae and Paramyxoviridae in the order Mononegavirales. P is a self-associated oligomer and forms complexes with the large viral polymerase protein (L), the nucleocapsid protein (N), and the assembled nucleocapsid. P from different viruses has shown structural diversities even though their essential functions are the same. We systematically mapped the domains in mumps virus (MuV) P and investigated their interactions with nucleocapsid-like particles (NLPs). Similar to other P proteins, MuV P contains N-terminal, central, and C-terminal domains with flexible linkers between neighboring domains. By pulldown assays, we discovered that in addition to the previously proposed nucleocapsid binding domain (residues 343-391), the N-terminal region of MuV P (residues 1-194) could also bind NLP. Further analysis of binding kinetics was conducted using surface plasmon resonance. This is the first observation that both the N- and C-terminal regions of a negative strand RNA virus P are involved in binding the nucleocapsid. Additionally, we defined the oligomerization domain (POD) of MuV P as residues 213-277 and determined its crystal structure. The tetrameric MuV POD is formed by one pair of long parallel alpha-helices with another pair in opposite orientation. Unlike the parallel orientation of each alpha-helix in the tetramer of Sendai virus POD, this represents a novel orientation of a POD where both the N- and C-terminal domains are at either end of the tetramer. This is consistent with the observation that both the N- and C-terminal domains are involved in binding the nucleocapsid.

	~~==About this Structure==~~		Structural and Functional Characterization of the Mumps Virus Phosphoprotein.,Cox R, Green TJ, Purushotham S, Deivanayagam C, Bedwell GJ, Prevelige PE, Luo M J Virol. 2013 May 1. PMID:23637399<ref>PMID:23637399</ref>
	~~[[4eij]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mumps_virus Mumps virus]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:023637399</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Mumps virus]]		[[Category: Mumps virus]]
	[[Category: Cox, R.]]		[[Category: Cox, R]]
	[[Category: Green, T J.]]		[[Category: Green, T J]]
	[[Category: Luo, M.]]		[[Category: Luo, M]]
	[[Category: Oligomerization]]		[[Category: Oligomerization]]
	[[Category: Replication]]		[[Category: Replication]]

OCA at 16:30, 19 June 2013

2013-06-19T16:30:38Z

← Older revision		Revision as of 16:30, 19 June 2013
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	{{STRUCTURE_4eij\| PDB=4eij \| SCENE= }}		{{STRUCTURE_4eij\| PDB=4eij \| SCENE= }}
	===Structure of the Mumps virus phosphoprotein oligomerization domain===		===Structure of the Mumps virus phosphoprotein oligomerization domain===
			{{ABSTRACT_PUBMED_23637399}}

	==About this Structure==		==About this Structure==
	[[4eij]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mumps_virus Mumps virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA].		[[4eij]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mumps_virus Mumps virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA].

			==Reference==
			<ref group="xtra">PMID:023637399</ref><references group="xtra"/><references/>
	[[Category: Mumps virus]]		[[Category: Mumps virus]]
	[[Category: Cox, R.]]		[[Category: Cox, R.]]

OCA at 07:49, 8 May 2013

2013-05-08T07:49:12Z

← Older revision		Revision as of 07:49, 8 May 2013
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		{{STRUCTURE_4eij\| PDB=4eij \| SCENE= }}
			===Structure of the Mumps virus phosphoprotein oligomerization domain===

	~~The entry~~ 4eij is ~~ON HOLD until May 06 2014~~		==About this Structure==
			[[4eij]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mumps_virus Mumps virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIJ OCA].
	~~Authors~~: Cox, R., Green, T.J., Luo, M.		[[Category: Mumps virus]]
			[[Category: Cox, R.]]
	~~Description~~: ~~Structure of the Mumps virus phosphoprotein oligomerization domain~~		[[Category: Green, T J.]]
			[[Category: Luo, M.]]
			[[Category: Oligomerization]]
			[[Category: Replication]]

OCA at 16:07, 20 March 2013

2013-03-20T16:07:08Z

← Older revision		Revision as of 16:07, 20 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eij is ON HOLD until ~~Paper Publication~~		The entry 4eij is ON HOLD until May 06 2014

	Authors: Cox, R., Green, T.J., Luo, M.		Authors: Cox, R., Green, T.J., Luo, M.

	Description: Structure of the Mumps virus phosphoprotein oligomerization domain		Description: Structure of the Mumps virus phosphoprotein oligomerization domain

OCA at 08:47, 16 May 2012

2012-05-16T08:47:55Z

← Older revision		Revision as of 08:47, 16 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eij is ON HOLD		The entry 4eij is ON HOLD until Paper Publication

	Authors: Cox, R., Green, T.J., Luo, M.		Authors: Cox, R., Green, T.J., Luo, M.

	Description: Structure of the Mumps virus phosphoprotein oligomerization domain		Description: Structure of the Mumps virus phosphoprotein oligomerization domain

OCA: Protected "4eij" [edit=sysop:move=sysop]

2012-04-11T06:44:22Z

Protected "4eij" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:44, 11 April 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4eij is ON HOLD Authors: Cox, R., Green, T.J., Luo, M. Description: Structure of the Mumps virus phosphoprotein oligomerization domain

2012-04-11T06:44:21Z

New page: '''Unreleased structure''' The entry 4eij is ON HOLD Authors: Cox, R., Green, T.J., Luo, M. Description: Structure of the Mumps virus phosphoprotein oligomerization domain

New page

'''Unreleased structure'''

The entry 4eij is ON HOLD

Authors: Cox, R., Green, T.J., Luo, M.

Description: Structure of the Mumps virus phosphoprotein oligomerization domain