4eip - Revision history

OCA at 11:04, 1 March 2024

2024-03-01T11:04:00Z

← Older revision		Revision as of 11:04, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.332Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [https://pdbe.org/4eip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [https://www.ebi.ac.uk/pdbsum/4eip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eip ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [https://pdbe.org/4eip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [https://www.ebi.ac.uk/pdbsum/4eip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eip ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q8KI25_LENAE Q8KI25_LENAE]]		[https://www.uniprot.org/uniprot/Q8KI25_LENAE Q8KI25_LENAE]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The indolocarbazole biosynthetic enzymes StaC, InkE, RebC, and AtmC mediate the degree of oxidation of chromopyrrolic acid on route to the natural products staurosporine, K252a, rebeccamycin, and AT2433-A1, respectively. Here, we show that StaC and InkE, which mediate a net 4-electron oxidation, bind FAD with a micromolar K(d), whereas RebC and AtmC, which mediate a net 8-electron oxidation, bind FAD with a nanomolar K(d) while displaying the same FAD redox properties. We further create RebC-10x, a RebC protein with ten StaC-like amino acid substitutions outside of previously characterized FAD-binding motifs and the complementary StaC-10x. We find that these mutations mediate both FAD affinity and product specificity, with RebC-10x displaying higher StaC activity than StaC itself. X-ray structures of this StaC catalyst identify the substrate of StaC as 7-carboxy-K252c and suggest a unique mechanism for this FAD-dependent enzyme.

	An Unusual Role for a Mobile Flavin in StaC-like Indolocarbazole Biosynthetic Enzymes.,Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan CL Chem Biol. 2012 Jul 27;19(7):855-65. PMID:22840773<ref>PMID:22840773</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4eip" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:13, 28 September 2022

2022-09-28T07:13:03Z

← Older revision		Revision as of 07:13, 28 September 2022
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	==Native and K252c bound RebC-10x==		==Native and K252c bound RebC-10x==
	<StructureSection load='4eip' size='340' side='right' caption='[[4eip]], [[Resolution\|resolution]] 2.33Å' scene=''>		<StructureSection load='4eip' size='340' side='right'caption='[[4eip]], [[Resolution\|resolution]] 2.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"nocardia_aerocolonigenes"_(shinobu_and_kawato_1960)_pridham_1970 "nocardia~~ aerocolonigenes~~" (shinobu and kawato 1960) pridham 1970~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2r0c\|2r0c]], [[2r0p\|2r0p]], [[4eiq\|4eiq]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [https://pdbe.org/4eip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [https://www.ebi.ac.uk/pdbsum/4eip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eip ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 "Nocardia aerocolonigenes" (Shinobu and Kawato 1960) Pridham 1970])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [~~http~~://pdbe.org/4eip PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eip PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eip ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/Q8KI25_LENAE Q8KI25_LENAE]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Drennan, C L~~]]		[[Category: Large Structures]]
	[[Category: ~~Elliott, S J~~]]		[[Category: Lentzea aerocolonigenes]]
	[[Category: ~~Goldman, P J~~]]		[[Category: Drennan CL]]
	[[Category: ~~Hamill, M J~~]]		[[Category: Elliott SJ]]
	[[Category: ~~Howard-Jones, A R~~]]		[[Category: Goldman PJ]]
	[[Category: ~~Ryan, K S~~]]		[[Category: Hamill MJ]]
	[[Category: ~~Walsh, C T~~]]		[[Category: Howard-Jones AR]]
	[[Category: ~~Flavin adenine dinucleotide~~]]		[[Category: Ryan KS]]
	[[Category: ~~Indolocarbazole]]~~		[[Category: Walsh CT]]
	~~[[Category: K252c]]~~
	~~[[Category: Monooxygenase]]~~
	~~[[Category: Oxidoreductase-oxidoreductase inhibitor complex~~]]

OCA at 14:03, 11 August 2016

2016-08-11T14:03:08Z

← Older revision		Revision as of 14:03, 11 August 2016
Line 1:		Line 1:

	==Native and K252c bound RebC-10x==		==Native and K252c bound RebC-10x==
	<StructureSection load='4eip' size='340' side='right' caption='[[4eip]], [[Resolution\|resolution]] 2.33Å' scene=''>		<StructureSection load='4eip' size='340' side='right' caption='[[4eip]], [[Resolution\|resolution]] 2.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lechevalieria_aerocolonigenes Lechevalieria~~ aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"nocardia_aerocolonigenes"_(shinobu_and_kawato_1960)_pridham_1970 "nocardia aerocolonigenes" (shinobu and kawato 1960) pridham 1970]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2r0c\|2r0c]], [[2r0p\|2r0p]], [[4eiq\|4eiq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2r0c\|2r0c]], [[2r0p\|2r0p]], [[4eiq\|4eiq]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 ~~Lechevalieria~~ aerocolonigenes])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 "Nocardia aerocolonigenes" (Shinobu and Kawato 1960) Pridham 1970])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [http://www.ebi.ac.uk/pdbsum/4eip PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [http://pdbe.org/4eip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [http://www.ebi.ac.uk/pdbsum/4eip PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eip ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eip" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Lechevalieria aerocolonigenes]]~~
	[[Category: Drennan, C L]]		[[Category: Drennan, C L]]
	[[Category: Elliott, S J]]		[[Category: Elliott, S J]]

OCA at 14:28, 4 January 2015

2015-01-04T14:28:49Z

← Older revision		Revision as of 14:28, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIP FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2r0c\|2r0c]], [[2r0p\|2r0p]], [[4eiq\|4eiq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2r0c\|2r0c]], [[2r0p\|2r0p]], [[4eiq\|4eiq]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [http://www.ebi.ac.uk/pdbsum/4eip PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [http://www.ebi.ac.uk/pdbsum/4eip PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Lechevalieria aerocolonigenes]]		[[Category: Lechevalieria aerocolonigenes]]
	[[Category: Drennan, C L.]]		[[Category: Drennan, C L]]
	[[Category: Elliott, S J.]]		[[Category: Elliott, S J]]
	[[Category: Goldman, P J.]]		[[Category: Goldman, P J]]
	[[Category: Hamill, M J.]]		[[Category: Hamill, M J]]
	[[Category: Howard-Jones, A R.]]		[[Category: Howard-Jones, A R]]
	[[Category: Ryan, K S.]]		[[Category: Ryan, K S]]
	[[Category: Walsh, C T.]]		[[Category: Walsh, C T]]
	[[Category: Flavin adenine dinucleotide]]		[[Category: Flavin adenine dinucleotide]]
	[[Category: Indolocarbazole]]		[[Category: Indolocarbazole]]

OCA at 09:23, 11 June 2014

2014-06-11T09:23:54Z

← Older revision		Revision as of 09:23, 11 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~4eip~~.~~jpg~~\|~~left~~\|~~200px~~]]		==Native and K252c bound RebC-10x==
			<StructureSection load='4eip' size='340' side='right' caption='[[4eip]], [[Resolution\|resolution]] 2.33Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIP FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=K2C:6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOL-5-ONE'>K2C</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2r0c\|2r0c]], [[2r0p\|2r0p]], [[4eiq\|4eiq]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eip RCSB], [http://www.ebi.ac.uk/pdbsum/4eip PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The indolocarbazole biosynthetic enzymes StaC, InkE, RebC, and AtmC mediate the degree of oxidation of chromopyrrolic acid on route to the natural products staurosporine, K252a, rebeccamycin, and AT2433-A1, respectively. Here, we show that StaC and InkE, which mediate a net 4-electron oxidation, bind FAD with a micromolar K(d), whereas RebC and AtmC, which mediate a net 8-electron oxidation, bind FAD with a nanomolar K(d) while displaying the same FAD redox properties. We further create RebC-10x, a RebC protein with ten StaC-like amino acid substitutions outside of previously characterized FAD-binding motifs and the complementary StaC-10x. We find that these mutations mediate both FAD affinity and product specificity, with RebC-10x displaying higher StaC activity than StaC itself. X-ray structures of this StaC catalyst identify the substrate of StaC as 7-carboxy-K252c and suggest a unique mechanism for this FAD-dependent enzyme.

	~~{{STRUCTURE_4eip\| PDB=4eip \| SCENE= }}~~		An Unusual Role for a Mobile Flavin in StaC-like Indolocarbazole Biosynthetic Enzymes.,Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan CL Chem Biol. 2012 Jul 27;19(7):855-65. PMID:22840773<ref>PMID:22840773</ref>

	~~===Native and K252c bound RebC-10x===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22840773}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4eip]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4EIP OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022840773<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Lechevalieria aerocolonigenes]]		[[Category: Lechevalieria aerocolonigenes]]
	[[Category: Drennan, C L.]]		[[Category: Drennan, C L.]]

OCA at 05:20, 8 August 2012

2012-08-08T05:20:27Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4eip.jpg|left|200px]]
-The entry 4eip is ON HOLD  until Paper Publication
+{{STRUCTURE_4eip|  PDB=4eip  |  SCENE=  }}
-Authors: Goldman, P.J., Ryan, K.S., Howard-Jones, A.R., Hamill, M.J., Elliott, S.J., Walsh, C.T., Drennan, C.L.
+===Native and K252c bound RebC-10x===
-Description: Native and K252c bound RebC-10x
+{{ABSTRACT_PUBMED_22840773}}

OCA at 08:53, 25 April 2012

2012-04-25T08:53:16Z

← Older revision		Revision as of 08:53, 25 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eip is ON HOLD		The entry 4eip is ON HOLD until Paper Publication

	Authors: Goldman, P.J., Ryan, K.S., Howard-Jones, A.R., Hamill, M.J., Elliott, S.J., Walsh, C.T., Drennan, C.L.		Authors: Goldman, P.J., Ryan, K.S., Howard-Jones, A.R., Hamill, M.J., Elliott, S.J., Walsh, C.T., Drennan, C.L.

	Description: Native and K252c bound RebC-10x		Description: Native and K252c bound RebC-10x

OCA: Protected "4eip" [edit=sysop:move=sysop]

2012-04-11T06:44:32Z

Protected "4eip" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:44, 11 April 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4eip is ON HOLD Authors: Goldman, P.J., Ryan, K.S., Howard-Jones, A.R., Hamill, M.J., Elliott, S.J., Walsh, C.T., Drennan, C.L. Description: Native...

2012-04-11T06:44:31Z

New page: '''Unreleased structure''' The entry 4eip is ON HOLD Authors: Goldman, P.J., Ryan, K.S., Howard-Jones, A.R., Hamill, M.J., Elliott, S.J., Walsh, C.T., Drennan, C.L. Description: Native...

New page

'''Unreleased structure'''

The entry 4eip is ON HOLD

Authors: Goldman, P.J., Ryan, K.S., Howard-Jones, A.R., Hamill, M.J., Elliott, S.J., Walsh, C.T., Drennan, C.L.

Description: Native and K252c bound RebC-10x