4eiv - Revision history

OCA at 15:02, 14 March 2024

2024-03-14T15:02:39Z

← Older revision		Revision as of 15:02, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.37Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [https://pdbe.org/4eiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [https://www.ebi.ac.uk/pdbsum/4eiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [https://pdbe.org/4eiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [https://www.ebi.ac.uk/pdbsum/4eiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/B9PVB4_TOXGV B9PVB4_TOXGV]]		[https://www.uniprot.org/uniprot/B9PVB4_TOXGV B9PVB4_TOXGV]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Parasites of the phylum Apicomplexa are highly successful pathogens of humans and animals world-wide. As obligate intracellular parasites, they have significant energy requirements for invasion and gliding motility that are supplied by various metabolic pathways. Aldolases have emerged as key enzymes involved in these pathways, and all apicomplexans express one or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA). Intriguingly, Toxoplasma gondii, a highly successful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent bradyzoite stage. While the importance of TgALD1 in glycolysis is well established and TgDERA is also likely to be involved in parasite metabolism, the detailed function of TgDPA remains elusive. To gain mechanistic insight into the function of different T. gondii aldolases, we first determined the crystal structures of TgALD1 and TgDPA. Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with divergent secondary structure elements. Structural comparison of TgALD1 and TgDPA with members of their respective enzyme families revealed that while the active site residues are conserved in TgALD1, key catalytic residues are absent in TgDPA. Consistent with this observation, biochemical assays showed that while TgALD1 was active on F16BP, TgDPA was inactive on dR5P. Intriguingly, both aldolases are competent to bind polymerized actin in vitro. Altogether, structural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization of the classic TIM barrel aldolase fold.

	Structural and functional divergence of the aldolase fold in Toxoplasma gondii.,Tonkin ML, Halavaty AS, Ramaswamy R, Ruan J, Igarashi M, Ngo HM, Boulanger MJ J Mol Biol. 2014 Oct 2. pii: S0022-2836(14)00518-X. doi:, 10.1016/j.jmb.2014.09.019. PMID:25284756<ref>PMID:25284756</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4eiv" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Aldolase 3D structures\|Aldolase 3D structures]]		*[[Aldolase 3D structures\|Aldolase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:13, 28 September 2022

2022-09-28T07:13:22Z

← Older revision		Revision as of 07:13, 28 September 2022
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	==1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==		==1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
	<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='4eiv' size='340' side='right'caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Toxgo Toxgo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EIV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [https://pdbe.org/4eiv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [https://www.ebi.ac.uk/pdbsum/4eiv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiv ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [~~http~~://pdbe.org/4eiv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eiv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiv ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/B9PVB4_TOXGV B9PVB4_TOXGV]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4eiv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4eiv" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Aldolase 3D structures\|Aldolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Deoxyribose-phosphate aldolase~~]]		[[Category: Large Structures]]
	[[Category: ~~Toxgo~~]]		[[Category: Toxoplasma gondii]]
	[[Category: Anderson~~, W F]]~~		[[Category: Anderson WF]]
	~~[[Category: Structural genomic~~]]		[[Category: Halavaty AS]]
	[[Category: Halavaty~~, A S~~]]		[[Category: Igarashi M]]
	[[Category: Igarashi, M]]		[[Category: Minasov G]]
	[[Category: Minasov, G]]		[[Category: Ngo H]]
	[[Category: Ngo, H]]		[[Category: Ruan J]]
	[[Category: Ruan, J]]		[[Category: Shuvalova L]]
	[[Category: Shuvalova, L]]		[[Category: Ueno A]]
	[[Category: Ueno, A]]
	~~[[Category: Bradyzoite]]~~
	~~[[Category: Brain cyst]]~~
	~~[[Category: Chemotherapy]]~~
	~~[[Category: Csgid]]~~
	~~[[Category: Lyase]]~~
	~~[[Category: Tim-barrel~~]]

OCA at 08:04, 15 November 2017

2017-11-15T08:04:56Z

← Older revision		Revision as of 08:04, 15 November 2017
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	</div>		</div>
	<div class="pdbe-citations 4eiv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4eiv" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Aldolase\|Aldolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:49, 15 December 2016

2016-12-15T19:49:25Z

← Older revision		Revision as of 19:49, 15 December 2016
Line 1:		Line 1:

	==1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==		==1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
	<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://pdbe.org/4eiv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eiv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 11:42, 4 March 2015

2015-03-04T11:42:47Z

← Older revision		Revision as of 11:42, 4 March 2015
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

			==See Also==
			*[[Aldolase\|Aldolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:10, 25 January 2015

2015-01-25T10:10:11Z

← Older revision		Revision as of 10:10, 25 January 2015
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	<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
			[[Category: Toxgo]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 07:34, 12 November 2014

2014-11-12T07:34:04Z

← Older revision		Revision as of 07:34, 12 November 2014
Line 2:		Line 2:
	<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>
	<tr~~><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~5811 TOXGO])</td></tr>~~		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	~~<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>		</table>
	<table>		<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Parasites of the phylum Apicomplexa are highly successful pathogens of humans and animals world-wide. As obligate intracellular parasites, they have significant energy requirements for invasion and gliding motility that are supplied by various metabolic pathways. Aldolases have emerged as key enzymes involved in these pathways, and all apicomplexans express one or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA). Intriguingly, Toxoplasma gondii, a highly successful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent bradyzoite stage. While the importance of TgALD1 in glycolysis is well established and TgDERA is also likely to be involved in parasite metabolism, the detailed function of TgDPA remains elusive. To gain mechanistic insight into the function of different T. gondii aldolases, we first determined the crystal structures of TgALD1 and TgDPA. Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with divergent secondary structure elements. Structural comparison of TgALD1 and TgDPA with members of their respective enzyme families revealed that while the active site residues are conserved in TgALD1, key catalytic residues are absent in TgDPA. Consistent with this observation, biochemical assays showed that while TgALD1 was active on F16BP, TgDPA was inactive on dR5P. Intriguingly, both aldolases are competent to bind polymerized actin in vitro. Altogether, structural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization of the classic TIM barrel aldolase fold.

			Structural and functional divergence of the aldolase fold in Toxoplasma gondii.,Tonkin ML, Halavaty AS, Ramaswamy R, Ruan J, Igarashi M, Ngo HM, Boulanger MJ J Mol Biol. 2014 Oct 2. pii: S0022-2836(14)00518-X. doi:, 10.1016/j.jmb.2014.09.019. PMID:25284756<ref>PMID:25284756</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
	~~[[Category: Toxgo]]~~		[[Category: Anderson, W F]]
	[[Category: Anderson, W F.]]		[[Category: Structural genomic]]
	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]		[[Category: Halavaty, A S]]
	[[Category: Halavaty, A S.]]		[[Category: Igarashi, M]]
	[[Category: Igarashi, M.]]		[[Category: Minasov, G]]
	[[Category: Minasov, G.]]		[[Category: Ngo, H]]
	[[Category: Ngo, H.]]		[[Category: Ruan, J]]
	[[Category: Ruan, J.]]		[[Category: Shuvalova, L]]
	[[Category: Shuvalova, L.]]		[[Category: Ueno, A]]
	[[Category: Ueno, A.]]
	[[Category: Bradyzoite]]		[[Category: Bradyzoite]]
	[[Category: Brain cyst]]		[[Category: Brain cyst]]
	~~[[Category: Center for structural genomics of infectious disease]]~~
	[[Category: Chemotherapy]]		[[Category: Chemotherapy]]
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: Lyase]]		[[Category: Lyase]]
	~~[[Category: Structural genomic]]~~
	[[Category: Tim-barrel]]		[[Category: Tim-barrel]]

OCA at 21:47, 1 October 2014

2014-10-01T21:47:09Z

← Older revision		Revision as of 21:47, 1 October 2014
Line 2:		Line 2:
	<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Toxoplasma_gondii Toxoplasma gondii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 ~~Toxoplasma gondii~~])</td></tr>		<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>		<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>
Line 12:		Line 12:
	</StructureSection>		</StructureSection>
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
	[[Category: ~~Toxoplasma gondii~~]]		[[Category: Toxgo]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]
	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]		[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]

OCA at 07:29, 5 June 2014

2014-06-05T07:29:18Z

← Older revision		Revision as of 07:29, 5 June 2014
Line 1:		Line 1:
	~~[[Image:4eiv.jpg\|left\|200px]]~~		==1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
			<StructureSection load='4eiv' size='340' side='right' caption='[[4eiv]], [[Resolution\|resolution]] 1.37Å' scene=''>
	~~<!--~~		== Structural highlights ==
	~~The line below this paragraph, containing "STRUCTURE_4eiv", creates the "Structure Box" on the page.~~		<table><tr><td colspan='2'>[[4eiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIV FirstGlance]. <br>
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qyq\|3qyq]]</td></tr>
	~~or leave the SCENE parameter empty for the default display.~~		<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGGT1_122370, TGME49_118750, TGVEG_010130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 Toxoplasma gondii])</td></tr>
	~~-->~~		<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	~~{{STRUCTURE_4eiv\| PDB=4eiv \| SCENE= }}~~		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiv RCSB], [http://www.ebi.ac.uk/pdbsum/4eiv PDBsum]</span></td></tr>
			<table>
	===1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49===		__TOC__
			</StructureSection>

	==~~About this Structure~~==
	[[4eiv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIV OCA].
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
	[[Category: Toxoplasma gondii]]		[[Category: Toxoplasma gondii]]

OCA at 06:17, 23 May 2012

2012-05-23T06:17:40Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4eiv.jpg|left|200px]]
-The entry 4eiv is ON HOLD
+<!--
-Authors: Halavaty, A.S., Ruan, J., Minasov, G., Shuvalova, L., Ueno, A., Igarashi, M., Ngo, H., Anderson, W.F., Center for Structural Genomics of Infectious Diseases (CSGID)
+===1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49===
-Description: 1.37 Angstrom resolution crystal structure of apo-form of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49