4ej3 - Revision history

OCA at 11:04, 1 March 2024

2024-03-01T11:04:03Z

← Older revision		Revision as of 11:04, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ej3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EJ3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ej3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EJ3 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [https://pdbe.org/4ej3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [https://www.ebi.ac.uk/pdbsum/4ej3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej3 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.52Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [https://pdbe.org/4ej3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [https://www.ebi.ac.uk/pdbsum/4ej3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CSE1_THET8 CSE1_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).		[https://www.uniprot.org/uniprot/CSE1_THET8 CSE1_THET8] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).

	==See Also==		==See Also==

OCA at 07:13, 28 September 2022

2022-09-28T07:13:37Z

← Older revision		Revision as of 07:13, 28 September 2022
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	==Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8==		==Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8==
	<StructureSection load='4ej3' size='340' side='right' caption='[[4ej3]], [[Resolution\|resolution]] 2.52Å' scene=''>		<StructureSection load='4ej3' size='340' side='right'caption='[[4ej3]], [[Resolution\|resolution]] 2.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ej3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EJ3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ej3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EJ3 FirstGlance]. <br>
	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHB188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [https://pdbe.org/4ej3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [https://www.ebi.ac.uk/pdbsum/4ej3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej3 ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [~~http~~://pdbe.org/4ej3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ej3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CSE1_THET8 CSE1_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).		[[https://www.uniprot.org/uniprot/CSE1_THET8 CSE1_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thet8~~]]		[[Category: Large Structures]]
	[[Category: ~~Huang, Q Q~~]]		[[Category: Thermus thermophilus HB8]]
	[[Category: ~~Cascade protein]]~~		[[Category: Huang QQ]]
	~~[[Category: Crispr]]~~
	~~[[Category: Signaling protein~~]]

OCA at 02:14, 5 August 2016

2016-08-05T02:14:52Z

← Older revision		Revision as of 02:14, 5 August 2016
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	==Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8==		==Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8==
	<StructureSection load='4ej3' size='340' side='right' caption='[[4ej3]], [[Resolution\|resolution]] 2.52Å' scene=''>		<StructureSection load='4ej3' size='340' side='right' caption='[[4ej3]], [[Resolution\|resolution]] 2.52Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ej3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus_hb8 Thermus thermophilus hb8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EJ3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ej3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EJ3 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHB188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 ~~Thermus thermophilus HB8~~])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHB188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [http://pdbe.org/4ej3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~Q53VY1_THET8 Q53VY1_THET8~~]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).		[[http://www.uniprot.org/uniprot/CSE1_THET8 CSE1_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).

	==See Also==		==See Also==
Line 13:		Line 14:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thermus thermophilus hb8~~]]		[[Category: Thet8]]
	[[Category: Huang, Q Q]]		[[Category: Huang, Q Q]]
	[[Category: Cascade protein]]		[[Category: Cascade protein]]
	[[Category: Crispr]]		[[Category: Crispr]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 15:00, 25 December 2014

2014-12-25T15:00:44Z

← Older revision		Revision as of 15:00, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej3 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q53VY1_THET8 Q53VY1_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA (By similarity).

			==See Also==
			*[[Endonuclease\|Endonuclease]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:38, 9 December 2014

2014-12-09T17:38:39Z

← Older revision		Revision as of 17:38, 9 December 2014
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	~~[[Image:4ej3.jpg\|left\|200px]]~~		==Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8==
			<StructureSection load='4ej3' size='340' side='right' caption='[[4ej3]], [[Resolution\|resolution]] 2.52Å' scene=''>
	~~{{STRUCTURE_4ej3\| PDB=4ej3 \| SCENE= }}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4ej3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EJ3 FirstGlance]. <br>
	===Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8===		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHB188 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ej3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej3 PDBsum]</span></td></tr>
			</table>
	==~~About this Structure~~==		__TOC__
	[[4ej3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA].		</StructureSection>
	[[Category: Thermus thermophilus hb8]]		[[Category: Thermus thermophilus hb8]]
	[[Category: Huang, Q Q.]]		[[Category: Huang, Q Q]]
	[[Category: Cascade protein]]		[[Category: Cascade protein]]
	[[Category: Crispr]]		[[Category: Crispr]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 13:57, 20 February 2013

2013-02-20T13:57:04Z

← Older revision		Revision as of 13:57, 20 February 2013
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4ej3.jpg\|left\|200px]]

	~~The entry~~ 4ej3 ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4ej3\| PDB=4ej3 \| SCENE= }}

	~~Authors: Huang, Q.Q.~~		===Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8===

	~~Description~~: ~~Crystal structure of a CRISPR associated protein~~ from Thermus thermophilus ~~HB8~~
			==About this Structure==
			[[4ej3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ3 OCA].
			[[Category: Thermus thermophilus hb8]]
			[[Category: Huang, Q Q.]]
			[[Category: Cascade protein]]
			[[Category: Crispr]]
			[[Category: Signaling protein]]

OCA at 08:53, 25 April 2012

2012-04-25T08:53:23Z

← Older revision		Revision as of 08:53, 25 April 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4ej3 is ON HOLD		The entry 4ej3 is ON HOLD until Paper Publication

	Authors: Huang, ~~Qingqiu~~		Authors: Huang, Q.Q.

	Description: Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8		Description: Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8

OCA: Protected "4ej3" [edit=sysop:move=sysop]

2012-04-18T07:32:22Z

Protected "4ej3" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:32, 18 April 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4ej3 is ON HOLD Authors: Huang, Qingqiu Description: Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8

2012-04-18T07:32:21Z

New page: '''Unreleased structure''' The entry 4ej3 is ON HOLD Authors: Huang, Qingqiu Description: Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8

New page

'''Unreleased structure'''

The entry 4ej3 is ON HOLD

Authors: Huang, Qingqiu

Description: Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8