4ej4 - Revision history

OCA at 09:58, 30 October 2024

2024-10-30T09:58:03Z

← Older revision		Revision as of 09:58, 30 October 2024
Line 9:		Line 9:
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/OPRD_MOUSE OPRD_MOUSE] G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:10677041</ref> <ref>PMID:1334555</ref> <ref>PMID:1335167</ref> ~~[https~~:~~//www~~.~~uniprot~~.~~org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity~~ that ~~degrades host peptidoglycans~~ and ~~participates with~~ the ~~holin and spanin proteins in~~ the ~~sequential events which lead~~ to the ~~programmed host cell lysis releasing the mature viral particles~~. ~~Once~~ the ~~holin has permeabilized~~ the ~~host cell membrane~~, the ~~endolysin~~ can ~~reach~~ the ~~periplasm~~ and ~~break down~~ the ~~peptidoglycan layer~~.<ref>PMID:~~22389108~~</ref>		[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> [https://www.uniprot.org/uniprot/OPRD_MOUSE OPRD_MOUSE] G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:10677041</ref> <ref>PMID:1334555</ref> <ref>PMID:1335167</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The opioid receptor family comprises three members, the micro-, delta- and kappa-opioid receptors, which respond to classical opioid alkaloids such as morphine and heroin as well as to endogenous peptide ligands like endorphins. They belong to the G-protein-coupled receptor (GPCR) superfamily, and are excellent therapeutic targets for pain control. The delta-opioid receptor (delta-OR) has a role in analgesia, as well as in other neurological functions that remain poorly understood. The structures of the micro-OR and kappa-OR have recently been solved. Here we report the crystal structure of the mouse delta-OR, bound to the subtype-selective antagonist naltrindole. Together with the structures of the micro-OR and kappa-OR, the delta-OR structure provides insights into conserved elements of opioid ligand recognition while also revealing structural features associated with ligand-subtype selectivity. The binding pocket of opioid receptors can be divided into two distinct regions. Whereas the lower part of this pocket is highly conserved among opioid receptors, the upper part contains divergent residues that confer subtype selectivity. This provides a structural explanation and validation for the 'message-address' model of opioid receptor pharmacology, in which distinct 'message' (efficacy) and 'address' (selectivity) determinants are contained within a single ligand. Comparison of the address region of the delta-OR with other GPCRs reveals that this structural organization may be a more general phenomenon, extending to other GPCR families as well.

			Structure of the delta-opioid receptor bound to naltrindole.,Granier S, Manglik A, Kruse AC, Kobilka TS, Thian FS, Weis WI, Kobilka BK Nature. 2012 May 16;485(7398):400-4. doi: 10.1038/nature11111. PMID:22596164<ref>PMID:22596164</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4ej4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 15:02, 14 March 2024

2024-03-14T15:02:47Z

← Older revision		Revision as of 15:02, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ej4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EJ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ej4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EJ4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EJ4:(4BS,8R,8AS,14BR)-7-(CYCLOPROPYLMETHYL)-5,6,7,8,14,14B-HEXAHYDRO-4,8-METHANO[1]BENZOFURO[2,3-A]PYRIDO[4,3-B]CARBAZOLE-1,8A(9H)-DIOL'>EJ4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EJ4:(4BS,8R,8AS,14BR)-7-(CYCLOPROPYLMETHYL)-5,6,7,8,14,14B-HEXAHYDRO-4,8-METHANO[1]BENZOFURO[2,3-A]PYRIDO[4,3-B]CARBAZOLE-1,8A(9H)-DIOL'>EJ4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej4 OCA], [https://pdbe.org/4ej4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ej4 RCSB], [https://www.ebi.ac.uk/pdbsum/4ej4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej4 OCA], [https://pdbe.org/4ej4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ej4 RCSB], [https://www.ebi.ac.uk/pdbsum/4ej4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/OPRD_MOUSE OPRD_MOUSE]] G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:10677041</ref> <ref>PMID:1334555</ref> <ref>PMID:1335167</ref> [[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>		[https://www.uniprot.org/uniprot/OPRD_MOUSE OPRD_MOUSE] G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:10677041</ref> <ref>PMID:1334555</ref> <ref>PMID:1335167</ref> [https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The opioid receptor family comprises three members, the micro-, delta- and kappa-opioid receptors, which respond to classical opioid alkaloids such as morphine and heroin as well as to endogenous peptide ligands like endorphins. They belong to the G-protein-coupled receptor (GPCR) superfamily, and are excellent therapeutic targets for pain control. The delta-opioid receptor (delta-OR) has a role in analgesia, as well as in other neurological functions that remain poorly understood. The structures of the micro-OR and kappa-OR have recently been solved. Here we report the crystal structure of the mouse delta-OR, bound to the subtype-selective antagonist naltrindole. Together with the structures of the micro-OR and kappa-OR, the delta-OR structure provides insights into conserved elements of opioid ligand recognition while also revealing structural features associated with ligand-subtype selectivity. The binding pocket of opioid receptors can be divided into two distinct regions. Whereas the lower part of this pocket is highly conserved among opioid receptors, the upper part contains divergent residues that confer subtype selectivity. This provides a structural explanation and validation for the 'message-address' model of opioid receptor pharmacology, in which distinct 'message' (efficacy) and 'address' (selectivity) determinants are contained within a single ligand. Comparison of the address region of the delta-OR with other GPCRs reveals that this structural organization may be a more general phenomenon, extending to other GPCR families as well.

	Structure of the delta-opioid receptor bound to naltrindole.,Granier S, Manglik A, Kruse AC, Kobilka TS, Thian FS, Weis WI, Kobilka BK Nature. 2012 May 16;485(7398):400-4. doi: 10.1038/nature11111. PMID:22596164<ref>PMID:22596164</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4ej4" style="background-color:#fffaf0;"></div~~>

	==See Also==		==See Also==

OCA at 07:13, 28 September 2022

2022-09-28T07:13:38Z

← Older revision		Revision as of 07:13, 28 September 2022
Line 1:		Line 1:

	==Structure of the delta opioid receptor bound to naltrindole==		==Structure of the delta opioid receptor bound to naltrindole==
	<StructureSection load='4ej4' size='340' side='right' caption='[[4ej4]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='4ej4' size='340' side='right'caption='[[4ej4]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ej4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EJ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ej4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EJ4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EJ4:(4BS,8R,8AS,14BR)-7-(CYCLOPROPYLMETHYL)-5,6,7,8,14,14B-HEXAHYDRO-4,8-METHANO[1]BENZOFURO[2,3-A]PYRIDO[4,3-B]CARBAZOLE-1,8A(9H)-DIOL'>EJ4</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EJ4:(4BS,8R,8AS,14BR)-7-(CYCLOPROPYLMETHYL)-5,6,7,8,14,14B-HEXAHYDRO-4,8-METHANO[1]BENZOFURO[2,3-A]PYRIDO[4,3-B]CARBAZOLE-1,8A(9H)-DIOL'>EJ4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Oprd1, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej4 OCA], [https://pdbe.org/4ej4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ej4 RCSB], [https://www.ebi.ac.uk/pdbsum/4ej4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej4 ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej4 OCA], [~~http~~://pdbe.org/4ej4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ej4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ej4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/OPRD_MOUSE OPRD_MOUSE]] G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:10677041</ref> <ref>PMID:1334555</ref> <ref>PMID:1335167</ref>		[[https://www.uniprot.org/uniprot/OPRD_MOUSE OPRD_MOUSE]] G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:10677041</ref> <ref>PMID:1334555</ref> <ref>PMID:1335167</ref> [[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
	*[[Delta opioid receptor\|Delta opioid receptor]]
	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]		*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
			*[[Opioid receptor\|Opioid receptor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Escherichia virus T4]]
	[[Category: ~~Lysozyme~~]]		[[Category: Large Structures]]
	[[Category: Granier, S]]		[[Category: Mus musculus]]
	[[Category: Kobilka~~, B K~~]]		[[Category: Granier S]]
	[[Category: Kobilka~~, T S~~]]		[[Category: Kobilka BK]]
	[[Category: Kruse~~, A C~~]]		[[Category: Kobilka TS]]
	[[Category: Manglik, A]]		[[Category: Kruse AC]]
	[[Category: Thian~~, F S~~]]		[[Category: Manglik A]]
	[[Category: Weis~~, W I]]~~		[[Category: Thian FS]]
	~~[[Category: Transmembrane receptor]]~~		[[Category: Weis WI]]
	~~[[Category: G-protein coupled receptor]]~~
	~~[[Category: Hydrolase-antagonist complex]]~~
	~~[[Category: Opioid receptor]]~~
	~~[[Category: Signaling protein~~]]

OCA at 22:26, 15 November 2017

2017-11-15T22:26:45Z

← Older revision		Revision as of 22:26, 15 November 2017
Line 23:		Line 23:
	==See Also==		==See Also==
	*[[Delta opioid receptor\|Delta opioid receptor]]		*[[Delta opioid receptor\|Delta opioid receptor]]
	*[[G protein-coupled receptor\|G protein-coupled receptor]]
	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]		*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	*[[Opioid receptor\|Opioid receptor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:23, 11 August 2016

2016-08-11T11:23:55Z

← Older revision		Revision as of 11:23, 11 August 2016
Line 1:		Line 1:

	==Structure of the delta opioid receptor bound to naltrindole==		==Structure of the delta opioid receptor bound to naltrindole==
	<StructureSection load='4ej4' size='340' side='right' caption='[[4ej4]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='4ej4' size='340' side='right' caption='[[4ej4]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ej4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EJ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ej4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EJ4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EJ4:(4BS,8R,8AS,14BR)-7-(CYCLOPROPYLMETHYL)-5,6,7,8,14,14B-HEXAHYDRO-4,8-METHANO[1]BENZOFURO[2,3-A]PYRIDO[4,3-B]CARBAZOLE-1,8A(9H)-DIOL'>EJ4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EJ4:(4BS,8R,8AS,14BR)-7-(CYCLOPROPYLMETHYL)-5,6,7,8,14,14B-HEXAHYDRO-4,8-METHANO[1]BENZOFURO[2,3-A]PYRIDO[4,3-B]CARBAZOLE-1,8A(9H)-DIOL'>EJ4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Oprd1, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Oprd1, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ej4 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej4 OCA], [http://pdbe.org/4ej4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ej4 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ej4 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/OPRD_MOUSE OPRD_MOUSE]] G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.<ref>PMID:10677041</ref> <ref>PMID:1334555</ref> <ref>PMID:1335167</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4ej4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
			*[[Delta opioid receptor\|Delta opioid receptor]]
	*[[G protein-coupled receptor\|G protein-coupled receptor]]		*[[G protein-coupled receptor\|G protein-coupled receptor]]
	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]		*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
Line 25:		Line 30:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Lk3 transgenic mice]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	~~[[Category: Mus musculus]]~~
	[[Category: Granier, S]]		[[Category: Granier, S]]
	[[Category: Kobilka, B K]]		[[Category: Kobilka, B K]]

OCA at 13:11, 5 January 2015

2015-01-05T13:11:30Z

← Older revision		Revision as of 13:11, 5 January 2015
Line 1:		Line 1:
	[[~~Image~~:~~4ej4~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of the delta opioid receptor bound to naltrindole==
			<StructureSection load='4ej4' size='340' side='right' caption='[[4ej4]], [[Resolution\|resolution]] 3.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4ej4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EJ4 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EJ4:(4BS,8R,8AS,14BR)-7-(CYCLOPROPYLMETHYL)-5,6,7,8,14,14B-HEXAHYDRO-4,8-METHANO[1]BENZOFURO[2,3-A]PYRIDO[4,3-B]CARBAZOLE-1,8A(9H)-DIOL'>EJ4</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Oprd1, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ej4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ej4 RCSB], [http://www.ebi.ac.uk/pdbsum/4ej4 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The opioid receptor family comprises three members, the micro-, delta- and kappa-opioid receptors, which respond to classical opioid alkaloids such as morphine and heroin as well as to endogenous peptide ligands like endorphins. They belong to the G-protein-coupled receptor (GPCR) superfamily, and are excellent therapeutic targets for pain control. The delta-opioid receptor (delta-OR) has a role in analgesia, as well as in other neurological functions that remain poorly understood. The structures of the micro-OR and kappa-OR have recently been solved. Here we report the crystal structure of the mouse delta-OR, bound to the subtype-selective antagonist naltrindole. Together with the structures of the micro-OR and kappa-OR, the delta-OR structure provides insights into conserved elements of opioid ligand recognition while also revealing structural features associated with ligand-subtype selectivity. The binding pocket of opioid receptors can be divided into two distinct regions. Whereas the lower part of this pocket is highly conserved among opioid receptors, the upper part contains divergent residues that confer subtype selectivity. This provides a structural explanation and validation for the 'message-address' model of opioid receptor pharmacology, in which distinct 'message' (efficacy) and 'address' (selectivity) determinants are contained within a single ligand. Comparison of the address region of the delta-OR with other GPCRs reveals that this structural organization may be a more general phenomenon, extending to other GPCR families as well.

	~~{{STRUCTURE_4ej4\| PDB=4ej4 \| SCENE= }}~~		Structure of the delta-opioid receptor bound to naltrindole.,Granier S, Manglik A, Kruse AC, Kobilka TS, Thian FS, Weis WI, Kobilka BK Nature. 2012 May 16;485(7398):400-4. doi: 10.1038/nature11111. PMID:22596164<ref>PMID:22596164</ref>

	~~===Structure~~ of the ~~delta opioid receptor bound to naltrindole===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22596164}}~~

	~~==About this Structure==~~
	~~[[4ej4]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=4EJ4 OCA].~~

	==See Also==		==See Also==
	*[[G protein-coupled receptor\|G protein-coupled receptor]]		*[[G protein-coupled receptor\|G protein-coupled receptor]]
			*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	==~~Reference~~==		*[[Opioid receptor\|Opioid receptor]]
	<~~ref group="xtra">PMID:022596164<~~/~~ref~~><~~references group="xtra"~~/>		== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Granier, S.]]		[[Category: Granier, S]]
	[[Category: Kobilka, B K.]]		[[Category: Kobilka, B K]]
	[[Category: Kobilka, T S.]]		[[Category: Kobilka, T S]]
	[[Category: Kruse, A C.]]		[[Category: Kruse, A C]]
	[[Category: Manglik, A.]]		[[Category: Manglik, A]]
	[[Category: Thian, F S.]]		[[Category: Thian, F S]]
	[[Category: Weis, W I.]]		[[Category: Weis, W I]]
	[[Category: ~~7 transmembrane~~ receptor]]		[[Category: Transmembrane receptor]]
	[[Category: G-protein coupled receptor]]		[[Category: G-protein coupled receptor]]
	[[Category: Hydrolase-antagonist complex]]		[[Category: Hydrolase-antagonist complex]]
	[[Category: Opioid receptor]]		[[Category: Opioid receptor]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 10:55, 20 October 2012

2012-10-20T10:55:20Z

← Older revision		Revision as of 10:55, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[4ej4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus~~,_enterobacteria_phage_t4~~ Mus musculus~~, enterobacteria phage t4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA].		[[4ej4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA].

			==See Also==
			*[[G protein-coupled receptor\|G protein-coupled receptor]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:022596164</ref><references group="xtra"/>		<ref group="xtra">PMID:022596164</ref><references group="xtra"/>
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Mus musculus~~, enterobacteria phage t4~~]]		[[Category: Mus musculus]]
	[[Category: Granier, S.]]		[[Category: Granier, S.]]
	[[Category: Kobilka, B K.]]		[[Category: Kobilka, B K.]]

OCA at 04:50, 27 June 2012

2012-06-27T04:50:28Z

← Older revision		Revision as of 04:50, 27 June 2012
Line 1:		Line 1:
	[[Image:4ej4.~~jpg~~\|left\|200px]]		[[Image:4ej4.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4ej4", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_4ej4\| PDB=4ej4 \| SCENE= }}		{{STRUCTURE_4ej4\| PDB=4ej4 \| SCENE= }}

	===Structure of the delta opioid receptor bound to naltrindole===		===Structure of the delta opioid receptor bound to naltrindole===

			{{ABSTRACT_PUBMED_22596164}}

	==About this Structure==		==About this Structure==
	[[4ej4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus,_enterobacteria_phage_t4 Mus musculus, enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA].		[[4ej4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus,_enterobacteria_phage_t4 Mus musculus, enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EJ4 OCA].

			==Reference==
			<ref group="xtra">PMID:022596164</ref><references group="xtra"/>
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Mus musculus, enterobacteria phage t4]]		[[Category: Mus musculus, enterobacteria phage t4]]

OCA at 09:24, 16 May 2012

2012-05-16T09:24:55Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4ej4.jpg|left|200px]]
-The entry 4ej4 is ON HOLD  until Paper Publication
+<!--
-Authors: Granier, S., Manglik, A., Kruse, A.C., Kobilka, T.S., Thian, F.S., Weis, W.I., Kobilka, B.K.
+===Structure of the delta opioid receptor bound to naltrindole===
-Description: Structure of the delta opioid receptor bound to naltrindole

OCA at 08:47, 9 May 2012

2012-05-09T08:47:07Z

← Older revision		Revision as of 08:47, 9 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4ej4 is ON HOLD		The entry 4ej4 is ON HOLD until Paper Publication

	Authors: Granier, S., Manglik, A., Kruse, A.C., Kobilka, T.S., Thian, F.S., Weis, W.I., Kobilka, B.K.		Authors: Granier, S., Manglik, A., Kruse, A.C., Kobilka, T.S., Thian, F.S., Weis, W.I., Kobilka, B.K.

	Description: Structure of the delta opioid receptor bound to naltrindole		Description: Structure of the delta opioid receptor bound to naltrindole