4eks - Revision history

OCA at 15:03, 14 March 2024

2024-03-14T15:03:47Z

← Older revision		Revision as of 15:03, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0R1:1,2-BENZISOXAZOLE'>0R1</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.64Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0R1:1,2-BENZISOXAZOLE'>0R1</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [https://pdbe.org/4eks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [https://www.ebi.ac.uk/pdbsum/4eks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eks ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [https://pdbe.org/4eks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [https://www.ebi.ac.uk/pdbsum/4eks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eks ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>		[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Synthetic cavitands and protein cavities have been widely studied as models for ligand recognition. Here we investigate the Met102 --> His substitution in the artificial L99A cavity in T4 lysozyme as a Kemp eliminase. The resulting enzyme had k(cat)/K(M) = 0.43 M(-1) s(-1) and a (k(cat)/K(M))/k(uncat) = 10(7) at pH 5.0. The crystal structure of this enzyme was determined at 1.30 A, as were the structures of four complexes of substrate and product analogs. The absence of ordered waters or hydrogen bonding interactions, and the presence of a common catalytic base (His102) in an otherwise hydrophobic, buried cavity, facilitated detailed analysis of the reaction mechanism and its optimization. Subsequent substitutions increased eliminase activity by an additional four-fold. As activity-enhancing substitutions were engineered into the cavity, protein stability decreased, consistent with the stability-function trade-off hypothesis. This and related model cavities may provide templates for studying protein design principles in radically simplified environments.

	~~Engineering a model protein cavity to catalyze the Kemp elimination.,Merski M, Shoichet BK Proc Natl Acad Sci U S A. 2012 Sep 17. PMID:22988064<ref>PMID:22988064</ref>~~

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4eks" style="background-color:#fffaf0;"></div~~>

	==See Also==		==See Also==

OCA at 07:15, 28 September 2022

2022-09-28T07:15:29Z

← Older revision		Revision as of 07:15, 28 September 2022
Line 1:		Line 1:

	==T4 Lysozyme L99A/M102H with Isoxazole Bound==		==T4 Lysozyme L99A/M102H with Isoxazole Bound==
	<StructureSection load='4eks' size='340' side='right' caption='[[4eks]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='4eks' size='340' side='right'caption='[[4eks]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eks]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpt4 Bpt4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0R1:1,2-BENZISOXAZOLE'>0R1</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0R1:1,2-BENZISOXAZOLE'>0R1</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e97\|4e97]], [[4ekp\|4ekp]], [[4ekq\|4ekq]], [[4ekr\|4ekr]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [https://pdbe.org/4eks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [https://www.ebi.ac.uk/pdbsum/4eks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eks ProSAT]</span></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>~~
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [~~http~~://pdbe.org/4eks PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4eks PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4eks ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/~~LYS_BPT4 LYS_BPT4~~]] ~~Helps~~ to ~~release~~ the mature ~~phage~~ particles ~~from~~ the cell ~~wall by breaking~~ down the peptidoglycan.		[[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bpt4~~]]		[[Category: Escherichia virus T4]]
	[[Category: ~~Lysozyme~~]]		[[Category: Large Structures]]
	[[Category: Merski, M]]		[[Category: Merski M]]
	[[Category: Shoichet~~, B K]]~~		[[Category: Shoichet BK]]
	~~[[Category: Alkylation of cys97]]~~
	~~[[Category: Hydrolase~~]]

OCA at 17:31, 4 August 2016

2016-08-04T17:31:13Z

← Older revision		Revision as of 17:31, 4 August 2016
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	==T4 Lysozyme L99A/M102H with Isoxazole Bound==		==T4 Lysozyme L99A/M102H with Isoxazole Bound==
	<StructureSection load='4eks' size='340' side='right' caption='[[4eks]], [[Resolution\|resolution]] 1.64Å' scene=''>		<StructureSection load='4eks' size='340' side='right' caption='[[4eks]], [[Resolution\|resolution]] 1.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4eks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_t4 Enterobacteria phage t4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4eks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0R1:1,2-BENZISOXAZOLE'>0R1</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0R1:1,2-BENZISOXAZOLE'>0R1</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e97\|4e97]], [[4ekp\|4ekp]], [[4ekq\|4ekq]], [[4ekr\|4ekr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e97\|4e97]], [[4ekp\|4ekp]], [[4ekq\|4ekq]], [[4ekr\|4ekr]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 ~~Enterobacteria phage T4~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [http://www.ebi.ac.uk/pdbsum/4eks PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [http://pdbe.org/4eks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [http://www.ebi.ac.uk/pdbsum/4eks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eks ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4eks" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Enterobacteria phage t4~~]]		[[Category: Bpt4]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Merski, M]]		[[Category: Merski, M]]

OCA at 17:37, 24 December 2014

2014-12-24T17:37:28Z

← Older revision		Revision as of 17:37, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [http://www.ebi.ac.uk/pdbsum/4eks PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [http://www.ebi.ac.uk/pdbsum/4eks PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/LYS_BPT4 LYS_BPT4]] Helps to release the mature phage particles from the cell wall by breaking down the peptidoglycan.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:37, 9 December 2014

2014-12-09T15:37:59Z

← Older revision		Revision as of 15:37, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~4eks~~.~~png~~\|~~left~~\|~~200px~~]]		==T4 Lysozyme L99A/M102H with Isoxazole Bound==
			<StructureSection load='4eks' size='340' side='right' caption='[[4eks]], [[Resolution\|resolution]] 1.64Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4eks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKS FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0R1:1,2-BENZISOXAZOLE'>0R1</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=HED:2-HYDROXYETHYL+DISULFIDE'>HED</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4e97\|4e97]], [[4ekp\|4ekp]], [[4ekq\|4ekq]], [[4ekr\|4ekr]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eks RCSB], [http://www.ebi.ac.uk/pdbsum/4eks PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Synthetic cavitands and protein cavities have been widely studied as models for ligand recognition. Here we investigate the Met102 --> His substitution in the artificial L99A cavity in T4 lysozyme as a Kemp eliminase. The resulting enzyme had k(cat)/K(M) = 0.43 M(-1) s(-1) and a (k(cat)/K(M))/k(uncat) = 10(7) at pH 5.0. The crystal structure of this enzyme was determined at 1.30 A, as were the structures of four complexes of substrate and product analogs. The absence of ordered waters or hydrogen bonding interactions, and the presence of a common catalytic base (His102) in an otherwise hydrophobic, buried cavity, facilitated detailed analysis of the reaction mechanism and its optimization. Subsequent substitutions increased eliminase activity by an additional four-fold. As activity-enhancing substitutions were engineered into the cavity, protein stability decreased, consistent with the stability-function trade-off hypothesis. This and related model cavities may provide templates for studying protein design principles in radically simplified environments.

	~~{{STRUCTURE_4eks\| PDB=4eks \| SCENE= }}~~		Engineering a model protein cavity to catalyze the Kemp elimination.,Merski M, Shoichet BK Proc Natl Acad Sci U S A. 2012 Sep 17. PMID:22988064<ref>PMID:22988064</ref>

	~~===T4 Lysozyme L99A~~/~~M102H with Isoxazole Bound===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22988064}}~~		==See Also==
			*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	==~~About this Structure~~==		== References ==
	[[~~4eks~~]] ~~is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA].~~		<references/>
			__TOC__
			</StructureSection>
	[[Category: Enterobacteria phage t4]]		[[Category: Enterobacteria phage t4]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Merski, M.]]		[[Category: Merski, M]]
	[[Category: Shoichet, B K.]]		[[Category: Shoichet, B K]]
	[[Category: Alkylation of cys97]]		[[Category: Alkylation of cys97]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 06:10, 3 October 2012

2012-10-03T06:10:43Z

← Older revision		Revision as of 06:10, 3 October 2012
Line 1:		Line 1:
	[[Image:4eks.~~jpg~~\|left\|200px]]		[[Image:4eks.png\|left\|200px]]

	{{STRUCTURE_4eks\| PDB=4eks \| SCENE= }}		{{STRUCTURE_4eks\| PDB=4eks \| SCENE= }}
Line 5:		Line 5:
	===T4 Lysozyme L99A/M102H with Isoxazole Bound===		===T4 Lysozyme L99A/M102H with Isoxazole Bound===

			{{ABSTRACT_PUBMED_22988064}}

	==About this Structure==		==About this Structure==

OCA at 05:37, 5 September 2012

2012-09-05T05:37:05Z

← Older revision		Revision as of 05:37, 5 September 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4eks.jpg\|left\|200px]]

	~~The entry~~ 4eks ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4eks\| PDB=4eks \| SCENE= }}

	~~Authors: Merski, M., Shoichet, B.K.~~		===T4 Lysozyme L99A/M102H with Isoxazole Bound===

	~~Description~~: T4 Lysozyme ~~L99A/M102H with Isoxazole Bound~~
			==About this Structure==
			[[4eks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKS OCA].
			[[Category: Enterobacteria phage t4]]
			[[Category: Lysozyme]]
			[[Category: Merski, M.]]
			[[Category: Shoichet, B K.]]
			[[Category: Alkylation of cys97]]
			[[Category: Hydrolase]]

OCA at 05:37, 23 May 2012

2012-05-23T05:37:36Z

← Older revision		Revision as of 05:37, 23 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4eks is ON HOLD		The entry 4eks is ON HOLD until Paper Publication

	Authors: Merski, M., Shoichet, B.K.		Authors: Merski, M., Shoichet, B.K.

	Description: T4 Lysozyme L99A/M102H with Isoxazole Bound		Description: T4 Lysozyme L99A/M102H with Isoxazole Bound

OCA at 18:56, 2 May 2012

2012-05-02T18:56:07Z

← Older revision		Revision as of 18:56, 2 May 2012
Line 3:		Line 3:
	The entry 4eks is ON HOLD		The entry 4eks is ON HOLD

	Authors: Merski, M., Shoichet, B. K.		Authors: Merski, M., Shoichet, B.K.

	Description: T4 Lysozyme L99A/M102H with Isoxazole Bound		Description: T4 Lysozyme L99A/M102H with Isoxazole Bound

OCA: Protected "4eks" [edit=sysop:move=sysop]

2012-04-18T07:34:00Z

Protected "4eks" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:34, 18 April 2012
(No difference)