4env - Revision history

OCA at 10:52, 13 August 2026

2026-08-13T10:52:28Z

← Older revision		Revision as of 10:52, 13 August 2026
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.		[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The main channel for H(2)O(2) access to the heme cavity in large subunit catalases is twice as long as in small subunit catalases and is divided into two distinct parts. Like small subunit catalases, the 15A of the channel adjacent to the heme has a predominantly hydrophobic surface with only weak water occupancy, but the next 15A extending to the protein surface is hydrophilic and contains a complex water matrix in multiple passages. At the approximate junction of these two sections are a conserved serine and glutamate that are hydrogen bonded and associated with H(2)O(2) in inactive variants. Mutation of these residues changed the dimensions of the channel, both enlarging and constricting it, and also changed the solvent occupancy in the hydrophobic, inner section of the main channel. Despite these structural changes and the prominent location of the residues in the channel, the variants exhibited less than a 2-fold change in the k(cat) and apparent K(M) kinetic constants. These results reflect the importance of the complex multi-passage structure of the main channel. Surprisingly, mutation of either the serine or glutamate to an aliphatic side chain interfered with heme oxidation to heme d.

			Influence of main channel structure on H(2)O(2) access to the heme cavity of catalase KatE of Escherichia coli.,Jha V, Chelikani P, Carpena X, Fita I, Loewen PC Arch Biochem Biophys. 2012 Oct 1;526(1):54-9. doi: 10.1016/j.abb.2012.06.010., Epub 2012 Jul 20. PMID:22820098<ref>PMID:22820098</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4env" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Catalase 3D structures\|Catalase 3D structures]]		*[[Catalase 3D structures\|Catalase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:05, 14 March 2024

2024-03-14T15:05:46Z

← Older revision		Revision as of 15:05, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4env]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ENV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4env]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ENV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [https://pdbe.org/4env PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [https://www.ebi.ac.uk/pdbsum/4env PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4env ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [https://pdbe.org/4env PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [https://www.ebi.ac.uk/pdbsum/4env PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4env ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.		[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The main channel for H(2)O(2) access to the heme cavity in large subunit catalases is twice as long as in small subunit catalases and is divided into two distinct parts. Like small subunit catalases, the 15A of the channel adjacent to the heme has a predominantly hydrophobic surface with only weak water occupancy, but the next 15A extending to the protein surface is hydrophilic and contains a complex water matrix in multiple passages. At the approximate junction of these two sections are a conserved serine and glutamate that are hydrogen bonded and associated with H(2)O(2) in inactive variants. Mutation of these residues changed the dimensions of the channel, both enlarging and constricting it, and also changed the solvent occupancy in the hydrophobic, inner section of the main channel. Despite these structural changes and the prominent location of the residues in the channel, the variants exhibited less than a 2-fold change in the k(cat) and apparent K(M) kinetic constants. These results reflect the importance of the complex multi-passage structure of the main channel. Surprisingly, mutation of either the serine or glutamate to an aliphatic side chain interfered with heme oxidation to heme d.

	Influence of main channel structure on H(2)O(2) access to the heme cavity of catalase KatE of Escherichia coli.,Jha V, Chelikani P, Carpena X, Fita I, Loewen PC Arch Biochem Biophys. 2012 Oct 1;526(1):54-9. doi: 10.1016/j.abb.2012.06.010., Epub 2012 Jul 20. PMID:22820098<ref>PMID:22820098</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4env" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Catalase 3D structures\|Catalase 3D structures]]		*[[Catalase 3D structures\|Catalase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:19, 28 September 2022

2022-09-28T07:19:57Z

← Older revision		Revision as of 07:19, 28 September 2022
Line 1:		Line 1:

	==Structure of the S234I variant of E. coli KatE==		==Structure of the S234I variant of E. coli KatE==
	<StructureSection load='4env' size='340' side='right' caption='[[4env]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4env' size='340' side='right'caption='[[4env]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4env]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ENV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4env]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ENV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4enp\|4enp]], [[4enq\|4enq]], [[4enr\|4enr]], [[4ens\|4ens]], [[4ent\|4ent]], [[4enu\|4enu]], [[4enw\|4enw]]</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [https://pdbe.org/4env PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [https://www.ebi.ac.uk/pdbsum/4env PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4env ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [~~http~~://pdbe.org/4env PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4env PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4env ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.		[[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
	*[[Catalase\|Catalase]]		*[[Catalase 3D structures\|Catalase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Catalase~~]]		[[Category: Escherichia coli K-12]]
	[[Category: ~~Ecoli~~]]		[[Category: Large Structures]]
	[[Category: Jha, V]]		[[Category: Jha V]]
	[[Category: Loewen~~, P C]]~~		[[Category: Loewen PC]]
	~~[[Category: Catalase fold]]~~
	~~[[Category: Kate]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: S234d variant~~]]

OCA at 08:23, 4 August 2016

2016-08-04T08:23:31Z

← Older revision		Revision as of 08:23, 4 August 2016
Line 1:		Line 1:

	==Structure of the S234I variant of E. coli KatE==		==Structure of the S234I variant of E. coli KatE==
	<StructureSection load='4env' size='340' side='right' caption='[[4env]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4env' size='340' side='right' caption='[[4env]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4env]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ENV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4env]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ENV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4enp\|4enp]], [[4enq\|4enq]], [[4enr\|4enr]], [[4ens\|4ens]], [[4ent\|4ent]], [[4enu\|4enu]], [[4enw\|4enw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4enp\|4enp]], [[4enq\|4enq]], [[4enr\|4enr]], [[4ens\|4ens]], [[4ent\|4ent]], [[4enu\|4enu]], [[4enw\|4enw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [http://www.ebi.ac.uk/pdbsum/4env PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [http://pdbe.org/4env PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [http://www.ebi.ac.uk/pdbsum/4env PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4env ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4env" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: ~~Escherichia coli k-12~~]]		[[Category: Ecoli]]
	[[Category: Jha, V]]		[[Category: Jha, V]]
	[[Category: Loewen, P C]]		[[Category: Loewen, P C]]

OCA at 07:37, 24 December 2014

2014-12-24T07:37:01Z

← Older revision		Revision as of 07:37, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [http://www.ebi.ac.uk/pdbsum/4env PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [http://www.ebi.ac.uk/pdbsum/4env PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 16:15, 9 December 2014

2014-12-09T16:15:34Z

← Older revision		Revision as of 16:15, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~4env~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of the S234I variant of E. coli KatE==
			<StructureSection load='4env' size='340' side='right' caption='[[4env]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4env]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ENV FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4enp\|4enp]], [[4enq\|4enq]], [[4enr\|4enr]], [[4ens\|4ens]], [[4ent\|4ent]], [[4enu\|4enu]], [[4enw\|4enw]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4env FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4env OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4env RCSB], [http://www.ebi.ac.uk/pdbsum/4env PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The main channel for H(2)O(2) access to the heme cavity in large subunit catalases is twice as long as in small subunit catalases and is divided into two distinct parts. Like small subunit catalases, the 15A of the channel adjacent to the heme has a predominantly hydrophobic surface with only weak water occupancy, but the next 15A extending to the protein surface is hydrophilic and contains a complex water matrix in multiple passages. At the approximate junction of these two sections are a conserved serine and glutamate that are hydrogen bonded and associated with H(2)O(2) in inactive variants. Mutation of these residues changed the dimensions of the channel, both enlarging and constricting it, and also changed the solvent occupancy in the hydrophobic, inner section of the main channel. Despite these structural changes and the prominent location of the residues in the channel, the variants exhibited less than a 2-fold change in the k(cat) and apparent K(M) kinetic constants. These results reflect the importance of the complex multi-passage structure of the main channel. Surprisingly, mutation of either the serine or glutamate to an aliphatic side chain interfered with heme oxidation to heme d.

	~~{{STRUCTURE_4env\| PDB=4env \| SCENE= }}~~		Influence of main channel structure on H(2)O(2) access to the heme cavity of catalase KatE of Escherichia coli.,Jha V, Chelikani P, Carpena X, Fita I, Loewen PC Arch Biochem Biophys. 2012 Oct 1;526(1):54-9. doi: 10.1016/j.abb.2012.06.010., Epub 2012 Jul 20. PMID:22820098<ref>PMID:22820098</ref>

	~~===Structure~~ of the ~~S234I variant~~ of E. ~~coli KatE===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22820098}}~~

	~~==About this Structure==~~
	~~[[4env]] is a 4 chain structure with sequence from [http:/~~/~~en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA].~~

	==See Also==		==See Also==
	*[[Catalase\|Catalase]]		*[[Catalase\|Catalase]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Jha, V.]]		[[Category: Jha, V]]
	[[Category: Loewen, P C.]]		[[Category: Loewen, P C]]
	[[Category: Catalase fold]]		[[Category: Catalase fold]]
	[[Category: Kate]]		[[Category: Kate]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: S234d variant]]		[[Category: S234d variant]]

OCA at 13:54, 27 February 2013

2013-02-27T13:54:41Z

← Older revision		Revision as of 13:54, 27 February 2013
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	===Structure of the S234I variant of E. coli KatE===		===Structure of the S234I variant of E. coli KatE===

			{{ABSTRACT_PUBMED_22820098}}

	==About this Structure==		==About this Structure==
	[[4env]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA].		[[4env]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA].

	==See Also==		==See Also==
	*[[Catalase\|Catalase]]		*[[Catalase\|Catalase]]
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli k-12]]
	[[Category: Jha, V.]]		[[Category: Jha, V.]]
	[[Category: Loewen, P C.]]		[[Category: Loewen, P C.]]

OCA at 05:30, 20 October 2012

2012-10-20T05:30:15Z

← Older revision		Revision as of 05:30, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
	[[4env]] is a 4 chain structure ~~of [[Catalase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA].		[[4env]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA].

	==See Also==		==See Also==

OCA at 10:15, 25 July 2012

2012-07-25T10:15:57Z

← Older revision		Revision as of 10:15, 25 July 2012
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	[[Image:4env.~~jpg~~\|left\|200px]]		[[Image:4env.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_4env", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_4env\| PDB=4env \| SCENE= }}		{{STRUCTURE_4env\| PDB=4env \| SCENE= }}

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	==About this Structure==		==About this Structure==
	[[4env]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA].		[[4env]] is a 4 chain structure of [[Catalase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ENV OCA].

			==See Also==
			*[[Catalase\|Catalase]]
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 19:31, 2 May 2012

2012-05-02T19:31:43Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4env.jpg|left|200px]]
-The entry 4env is ON HOLD
+<!--
-Authors: Loewen, P.C., Jha, V.
+===Structure of the S234I variant of E. coli KatE===
-Description: Structure of the S234I variant of E. coli KatE