4euk - Revision history

OCA at 11:06, 1 March 2024

2024-03-01T11:06:08Z

← Older revision		Revision as of 11:06, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4euk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EUK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4euk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EUK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4euk OCA], [https://pdbe.org/4euk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4euk RCSB], [https://www.ebi.ac.uk/pdbsum/4euk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4euk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4euk OCA], [https://pdbe.org/4euk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4euk RCSB], [https://www.ebi.ac.uk/pdbsum/4euk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4euk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/AHK5_ARATH AHK5_ARATH] Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Negative regulator of the ETR1-dependent abscisic acid (ABA) and ethylene signaling pathway that inhibits the root elongation. Promotes stomatal closure. Regulates stomatal opening by integrating multiple signals via hydrogen peroxide H(2)O(2) homeostasis in guard cells in an ABA-independent manner. May contribute to basal defense mechanisms by closing stomata in the presence of bacterial pathogens. Regulates both hormone levels and ROS production in response to stress. Required for full immunity to bacterial pathogen and necrotrophic fungus.<ref>PMID:17202180</ref> <ref>PMID:18077346</ref> <ref>PMID:18560512</ref> <ref>PMID:22256998</ref> <ref>PMID:22827948</ref>		[https://www.uniprot.org/uniprot/AHK5_ARATH AHK5_ARATH] Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Negative regulator of the ETR1-dependent abscisic acid (ABA) and ethylene signaling pathway that inhibits the root elongation. Promotes stomatal closure. Regulates stomatal opening by integrating multiple signals via hydrogen peroxide H(2)O(2) homeostasis in guard cells in an ABA-independent manner. May contribute to basal defense mechanisms by closing stomata in the presence of bacterial pathogens. Regulates both hormone levels and ROS production in response to stress. Required for full immunity to bacterial pathogen and necrotrophic fungus.<ref>PMID:17202180</ref> <ref>PMID:18077346</ref> <ref>PMID:18560512</ref> <ref>PMID:22256998</ref> <ref>PMID:22827948</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The multi-step phosphorelay (MSP) system defines a key signal transduction pathway in plants and many eukaryotes. In this system, external stimuli first lead to the activation of a histidine kinase, followed by transfer of a phosphoryl group from the receiver domain of the kinase (HK(RD)) to downstream, cytosolic phosphotransfer proteins (HPs). In order to establish the determinants of specificity for this signaling relay system, we have solved the first crystal structure of a plant HK(RD), AHK5(RD), in complex with one of its cognate HPs, AHP1. AHP1 binds AHK5(RD) via a prominent hydrogen bond docking ridge and a hydrophobic patch. These features are conserved among all AHP proteins, but differ significantly from other structurally characterized prokaryotic and eukaryotic HPs. Surface plasmon resonance experiments show that AHK5(RD) binds to AHP1-3 with similar, micromolar affinity, consistent with the transient nature of this signaling complex. Our correlation of structural and functional data provide the first insight, at the atomic level as well as with quantitative affinity data, into the molecular recognition events governing the MSP in plants.

	Structure-Function Analysis of Arabidopsis thaliana Histidine Kinase AHK5 Bound to Its Cognate Phosphotransfer Protein AHP1.,Bauer J, Reiss K, Veerabagu M, Heunemann M, Harter K, Stehle T Mol Plant. 2013 Jan 24. PMID:23132142<ref>PMID:23132142</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4euk" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:17, 7 October 2022

2022-10-07T04:17:18Z

← Older revision		Revision as of 04:17, 7 October 2022
Line 1:		Line 1:

	==Crystal structure==		==Crystal structure==
	<StructureSection load='4euk' size='340' side='right' caption='[[4euk]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4euk' size='340' side='right'caption='[[4euk]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4euk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EUK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4euk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EUK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AHK5, At5g10720, CKI2, MAJ23.80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), AHP1, At3g21510, ATHP3, MIL23.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4euk OCA], [https://pdbe.org/4euk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4euk RCSB], [https://www.ebi.ac.uk/pdbsum/4euk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4euk ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4euk OCA], [~~http~~://pdbe.org/4euk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4euk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4euk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4euk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/AHK5_ARATH AHK5_ARATH]] Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Negative regulator of the ETR1-dependent abscisic acid (ABA) and ethylene signaling pathway that inhibits the root elongation. Promotes stomatal closure. Regulates stomatal opening by integrating multiple signals via hydrogen peroxide H(2)O(2) homeostasis in guard cells in an ABA-independent manner. May contribute to basal defense mechanisms by closing stomata in the presence of bacterial pathogens. Regulates both hormone levels and ROS production in response to stress. Required for full immunity to bacterial pathogen and necrotrophic fungus.<ref>PMID:17202180</ref> <ref>PMID:18077346</ref> <ref>PMID:18560512</ref> <ref>PMID:22256998</ref> <ref>PMID:22827948</ref> [[http://www.uniprot.org/uniprot/AHP1_ARATH AHP1_ARATH]] Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay.<ref>PMID:10050311</ref> <ref>PMID:12068096</ref> <ref>PMID:14981318</ref> <ref>PMID:9804162</ref>		[https://www.uniprot.org/uniprot/AHK5_ARATH AHK5_ARATH] Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Negative regulator of the ETR1-dependent abscisic acid (ABA) and ethylene signaling pathway that inhibits the root elongation. Promotes stomatal closure. Regulates stomatal opening by integrating multiple signals via hydrogen peroxide H(2)O(2) homeostasis in guard cells in an ABA-independent manner. May contribute to basal defense mechanisms by closing stomata in the presence of bacterial pathogens. Regulates both hormone levels and ROS production in response to stress. Required for full immunity to bacterial pathogen and necrotrophic fungus.<ref>PMID:17202180</ref> <ref>PMID:18077346</ref> <ref>PMID:18560512</ref> <ref>PMID:22256998</ref> <ref>PMID:22827948</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Arath~~]]		[[Category: Arabidopsis thaliana]]
	[[Category: ~~Histidine kinase~~]]		[[Category: Large Structures]]
	[[Category: Bauer, J]]		[[Category: Bauer J]]
	[[Category: Stehle, T]]		[[Category: Stehle T]]
	~~[[Category: Multistep phosphorelay]]~~
	~~[[Category: Phosphotransfer protein]]~~
	~~[[Category: Plant signalling]]~~
	~~[[Category: Response regulator]]~~
	~~[[Category: Sensor histidine kinase]]~~
	~~[[Category: Signal transduction]]~~
	~~[[Category: Signaling protein]]~~
	~~[[Category: Transferase]]~~
	~~[[Category: Two-component system~~]]

OCA at 21:18, 5 August 2016

2016-08-05T21:18:51Z

← Older revision		Revision as of 21:18, 5 August 2016
Line 1:		Line 1:

	==Crystal structure==		==Crystal structure==
	<StructureSection load='4euk' size='340' side='right' caption='[[4euk]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4euk' size='340' side='right' caption='[[4euk]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4euk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EUK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4euk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EUK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AHK5, At5g10720, CKI2, MAJ23.80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~]), AHP1, At3g21510, ATHP3, MIL23.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AHK5, At5g10720, CKI2, MAJ23.80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH]), AHP1, At3g21510, ATHP3, MIL23.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4euk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4euk RCSB], [http://www.ebi.ac.uk/pdbsum/4euk PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4euk OCA], [http://pdbe.org/4euk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4euk RCSB], [http://www.ebi.ac.uk/pdbsum/4euk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4euk ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/AHK5_ARATH AHK5_ARATH]] Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Negative regulator of the ETR1-dependent abscisic acid (ABA) and ethylene signaling pathway that inhibits the root elongation. Promotes stomatal closure. Regulates stomatal opening by integrating multiple signals via hydrogen peroxide H(2)O(2) homeostasis in guard cells in an ABA-independent manner. May contribute to basal defense mechanisms by closing stomata in the presence of bacterial pathogens. Regulates both hormone levels and ROS production in response to stress. Required for full immunity to bacterial pathogen and necrotrophic fungus.<ref>PMID:17202180</ref> <ref>PMID:18077346</ref> <ref>PMID:18560512</ref> <ref>PMID:22256998</ref> <ref>PMID:22827948</ref> [[http://www.uniprot.org/uniprot/AHP1_ARATH AHP1_ARATH]] Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay.<ref>PMID:10050311</ref> <ref>PMID:12068096</ref> <ref>PMID:14981318</ref> <ref>PMID:9804162</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4euk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Arabidopsis thaliana~~]]		[[Category: Arath]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: Bauer, J]]		[[Category: Bauer, J]]

OCA at 11:00, 21 December 2014

2014-12-21T11:00:31Z

← Older revision		Revision as of 11:00, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4euk~~\| ~~PDB~~=4euk \| ~~SCENE~~= }}		==Crystal structure==
	===~~Crystal structure~~===		<StructureSection load='4euk' size='340' side='right' caption='[[4euk]], [[Resolution\|resolution]] 1.95Å' scene=''>
	~~{{ABSTRACT_PUBMED_23132142}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4euk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EUK FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AHK5, At5g10720, CKI2, MAJ23.80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), AHP1, At3g21510, ATHP3, MIL23.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4euk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4euk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4euk RCSB], [http://www.ebi.ac.uk/pdbsum/4euk PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The multi-step phosphorelay (MSP) system defines a key signal transduction pathway in plants and many eukaryotes. In this system, external stimuli first lead to the activation of a histidine kinase, followed by transfer of a phosphoryl group from the receiver domain of the kinase (HK(RD)) to downstream, cytosolic phosphotransfer proteins (HPs). In order to establish the determinants of specificity for this signaling relay system, we have solved the first crystal structure of a plant HK(RD), AHK5(RD), in complex with one of its cognate HPs, AHP1. AHP1 binds AHK5(RD) via a prominent hydrogen bond docking ridge and a hydrophobic patch. These features are conserved among all AHP proteins, but differ significantly from other structurally characterized prokaryotic and eukaryotic HPs. Surface plasmon resonance experiments show that AHK5(RD) binds to AHP1-3 with similar, micromolar affinity, consistent with the transient nature of this signaling complex. Our correlation of structural and functional data provide the first insight, at the atomic level as well as with quantitative affinity data, into the molecular recognition events governing the MSP in plants.

	~~==About this~~ Structure==		Structure-Function Analysis of Arabidopsis thaliana Histidine Kinase AHK5 Bound to Its Cognate Phosphotransfer Protein AHP1.,Bauer J, Reiss K, Veerabagu M, Heunemann M, Harter K, Stehle T Mol Plant. 2013 Jan 24. PMID:23132142<ref>PMID:23132142</ref>
	~~[[4euk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana~~ Arabidopsis thaliana]. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il/oca-bin~~/~~ocashort?id=4EUK OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:023132142</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: Bauer, J.]]		[[Category: Bauer, J]]
	[[Category: Stehle, T.]]		[[Category: Stehle, T]]
	[[Category: Multistep phosphorelay]]		[[Category: Multistep phosphorelay]]
	[[Category: Phosphotransfer protein]]		[[Category: Phosphotransfer protein]]

OCA at 18:22, 19 June 2013

2013-06-19T18:22:53Z

← Older revision		Revision as of 18:22, 19 June 2013
Line 1:		Line 1:
	~~[[Image:4euk.jpg\|left\|200px]]~~

	{{STRUCTURE_4euk\| PDB=4euk \| SCENE= }}		{{STRUCTURE_4euk\| PDB=4euk \| SCENE= }}

	===Crystal structure===		===Crystal structure===

	{{ABSTRACT_PUBMED_23132142}}		{{ABSTRACT_PUBMED_23132142}}

	==About this Structure==		==About this Structure==
	[[4euk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA].		[[4euk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EUK OCA].

			==Reference==
			<ref group="xtra">PMID:023132142</ref><references group="xtra"/><references/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]

OCA at 13:49, 20 February 2013

2013-02-20T13:49:01Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4euk.jpg|left|200px]]
-The entry 4euk is ON HOLD  until Paper Publication
+{{STRUCTURE_4euk|  PDB=4euk  |  SCENE=  }}
-Authors: Stehle, T., Bauer, J.
+===Crystal structure===
-Description: Crystal structure
+{{ABSTRACT_PUBMED_23132142}}

OCA at 05:39, 23 May 2012

2012-05-23T05:39:41Z

← Older revision		Revision as of 05:39, 23 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4euk is ON HOLD		The entry 4euk is ON HOLD until Paper Publication

	Authors: Stehle, T., Bauer, J.		Authors: Stehle, T., Bauer, J.

	Description: ~~crystal~~ structure		Description: Crystal structure

OCA: Protected "4euk" [edit=sysop:move=sysop]

2012-05-02T19:00:03Z

Protected "4euk" [edit=sysop:move=sysop]

← Older revision	Revision as of 19:00, 2 May 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4euk is ON HOLD Authors: Stehle, T., Bauer, J. Description: crystal structure

2012-05-02T19:00:02Z

New page: '''Unreleased structure''' The entry 4euk is ON HOLD Authors: Stehle, T., Bauer, J. Description: crystal structure

New page

'''Unreleased structure'''

The entry 4euk is ON HOLD

Authors: Stehle, T., Bauer, J.

Description: crystal structure