4evf - Revision history

OCA at 08:50, 20 March 2024

2024-03-20T08:50:17Z

← Older revision		Revision as of 08:50, 20 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EVF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evf OCA], [https://pdbe.org/4evf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4evf RCSB], [https://www.ebi.ac.uk/pdbsum/4evf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4evf ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evf OCA], [https://pdbe.org/4evf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4evf RCSB], [https://www.ebi.ac.uk/pdbsum/4evf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4evf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GIA1_GIAIN GIA1_GIAIN] Giardins are involved in parasite attachment to the intestinal mucosa and in the cytoskeletal disassembly and reassembly that marks the transition from infectious trophozoite to transmissible cyst. They may interact with other cytoskeletal proteins such as microtubules in the microribbons or crossbridges, to maintain the integrity of the ventral disk.		[https://www.uniprot.org/uniprot/GIA1_GIAIN GIA1_GIAIN] Giardins are involved in parasite attachment to the intestinal mucosa and in the cytoskeletal disassembly and reassembly that marks the transition from infectious trophozoite to transmissible cyst. They may interact with other cytoskeletal proteins such as microtubules in the microribbons or crossbridges, to maintain the integrity of the ventral disk.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Alpha-giardins constitute the annexin proteome (group E annexins) in the intestinal protozoan parasite Giardia and, as such, represent the evolutionary oldest eukaryotic annexins. The dominance of alpha-giardins in the cytoskeleton of Giardia with its greatly reduced actin content emphasises the importance of the alpha-giardins for the structural integrity of the parasite, which is particularly critical in the transformation stage between cyst and trophozoite. In this study, we report the crystal structures of the apo- and calcium-bound forms of alpha1-giardin, a protein localised to the plasma membrane of Giardia trophozoites that has recently been identified as a vaccine target. The calcium-bound crystal structure of alpha1-giardin revealed the presence of a type III site in the first repeat as known from other annexin structures, as well as a novel calcium binding site situated between repeats I and IV. By means of comparison, the crystal structures of three different alpha-giardins known to date indicate that these proteins engage different calcium coordination schemes, among each other, as well as compared to annexins of groups A-D. Evaluation of the calcium-dependent binding to acidic phosphoplipid membranes revealed that this process is not only mediated but also regulated by the environmental calcium concentration. Uniquely within the large family of annexins, alpha1-giardin disengages from the phospholipid membrane at high calcium concentrations possibly due to formation of a dimeric species. The observed behaviour is in line with changing calcium levels experienced by the parasite during excystation and may thus provide first insights into the molecular mechanisms underpinning the transformation and survival of the parasite in the host.

	Alpha-1 giardin is an annexin with highly unusual calcium-regulated mechanisms.,Weeratunga SK, Osman A, Hu NJ, Wang CK, Mason L, Svard S, Hope G, Jones MK, Hofmann A J Mol Biol. 2012 Oct 19;423(2):169-81. doi: 10.1016/j.jmb.2012.06.041. Epub 2012 , Jul 10. PMID:22796298<ref>PMID:22796298</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4evf" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:18, 7 October 2022

2022-10-07T04:18:39Z

← Older revision		Revision as of 04:18, 7 October 2022
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	<StructureSection load='4evf' size='340' side='right'caption='[[4evf]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4evf' size='340' side='right'caption='[[4evf]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Giain Giain~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EVF FirstGlance]. <br>
	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4evh\|4evh]]</td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evf OCA], [https://pdbe.org/4evf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4evf RCSB], [https://www.ebi.ac.uk/pdbsum/4evf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4evf ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evf OCA], [~~http~~://pdbe.org/4evf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4evf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4evf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4evf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/GIA1_GIAIN GIA1_GIAIN]] Giardins are involved in parasite attachment to the intestinal mucosa and in the cytoskeletal disassembly and reassembly that marks the transition from infectious trophozoite to transmissible cyst. They may interact with other cytoskeletal proteins such as microtubules in the microribbons or crossbridges, to maintain the integrity of the ventral disk.		[https://www.uniprot.org/uniprot/GIA1_GIAIN GIA1_GIAIN] Giardins are involved in parasite attachment to the intestinal mucosa and in the cytoskeletal disassembly and reassembly that marks the transition from infectious trophozoite to transmissible cyst. They may interact with other cytoskeletal proteins such as microtubules in the microribbons or crossbridges, to maintain the integrity of the ventral disk.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Giain~~]]		[[Category: Giardia intestinalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hofmann, A]]		[[Category: Hofmann A]]
	[[Category: Weeratunga, S]]		[[Category: Weeratunga S]]
	~~[[Category: Annexin]]~~
	~~[[Category: Calcium-binding protein]]~~
	~~[[Category: Membrane-binding protein]]~~
	~~[[Category: Metal binding protein~~]]

OCA at 07:50, 27 March 2019

2019-03-27T07:50:51Z

← Older revision		Revision as of 07:50, 27 March 2019
Line 1:		Line 1:

	==Crystal structure of apo alpha-1 giardin==		==Crystal structure of apo alpha-1 giardin==
	<StructureSection load='4evf' size='340' side='right' caption='[[4evf]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4evf' size='340' side='right'caption='[[4evf]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giain Giain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giain Giain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVF FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Giain]]		[[Category: Giain]]
			[[Category: Large Structures]]
	[[Category: Hofmann, A]]		[[Category: Hofmann, A]]
	[[Category: Weeratunga, S]]		[[Category: Weeratunga, S]]

OCA at 20:34, 4 August 2016

2016-08-04T20:34:09Z

← Older revision		Revision as of 20:34, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of apo alpha-1 giardin==		==Crystal structure of apo alpha-1 giardin==
	<StructureSection load='4evf' size='340' side='right' caption='[[4evf]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4evf' size='340' side='right' caption='[[4evf]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Giardia_intestinalis Giardia intestinalis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giain Giain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4evh\|4evh]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4evh\|4evh]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4evf RCSB], [http://www.ebi.ac.uk/pdbsum/4evf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evf OCA], [http://pdbe.org/4evf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4evf RCSB], [http://www.ebi.ac.uk/pdbsum/4evf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4evf ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GIA1_GIAIN GIA1_GIAIN]] Giardins are involved in parasite attachment to the intestinal mucosa and in the cytoskeletal disassembly and reassembly that marks the transition from infectious trophozoite to transmissible cyst. They may interact with other cytoskeletal proteins such as microtubules in the microribbons or crossbridges, to maintain the integrity of the ventral disk.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4evf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Giardia intestinalis~~]]		[[Category: Giain]]
	[[Category: Hofmann, A]]		[[Category: Hofmann, A]]
	[[Category: Weeratunga, S]]		[[Category: Weeratunga, S]]

OCA at 11:18, 21 December 2014

2014-12-21T11:18:22Z

← Older revision		Revision as of 11:18, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4evf~~\| ~~PDB~~=4evf \| ~~SCENE~~= }}		==Crystal structure of apo alpha-1 giardin==
	===~~Crystal~~ structure of ~~apo~~ alpha-1 giardin~~===~~		<StructureSection load='4evf' size='340' side='right' caption='[[4evf]], [[Resolution\|resolution]] 1.90Å' scene=''>
	~~{{ABSTRACT_PUBMED_22796298}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVF FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4evh\|4evh]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4evf RCSB], [http://www.ebi.ac.uk/pdbsum/4evf PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Alpha-giardins constitute the annexin proteome (group E annexins) in the intestinal protozoan parasite Giardia and, as such, represent the evolutionary oldest eukaryotic annexins. The dominance of alpha-giardins in the cytoskeleton of Giardia with its greatly reduced actin content emphasises the importance of the alpha-giardins for the structural integrity of the parasite, which is particularly critical in the transformation stage between cyst and trophozoite. In this study, we report the crystal structures of the apo- and calcium-bound forms of alpha1-giardin, a protein localised to the plasma membrane of Giardia trophozoites that has recently been identified as a vaccine target. The calcium-bound crystal structure of alpha1-giardin revealed the presence of a type III site in the first repeat as known from other annexin structures, as well as a novel calcium binding site situated between repeats I and IV. By means of comparison, the crystal structures of three different alpha-giardins known to date indicate that these proteins engage different calcium coordination schemes, among each other, as well as compared to annexins of groups A-D. Evaluation of the calcium-dependent binding to acidic phosphoplipid membranes revealed that this process is not only mediated but also regulated by the environmental calcium concentration. Uniquely within the large family of annexins, alpha1-giardin disengages from the phospholipid membrane at high calcium concentrations possibly due to formation of a dimeric species. The observed behaviour is in line with changing calcium levels experienced by the parasite during excystation and may thus provide first insights into the molecular mechanisms underpinning the transformation and survival of the parasite in the host.

	~~==About this Structure==~~		Alpha-1 giardin is an annexin with highly unusual calcium-regulated mechanisms.,Weeratunga SK, Osman A, Hu NJ, Wang CK, Mason L, Svard S, Hope G, Jones MK, Hofmann A J Mol Biol. 2012 Oct 19;423(2):169-81. doi: 10.1016/j.jmb.2012.06.041. Epub 2012 , Jul 10. PMID:22796298<ref>PMID:22796298</ref>
	~~[[4evf]]~~ is ~~a 1 chain structure~~ with ~~sequence from [http~~:/~~/en~~.~~wikipedia~~.~~org/wiki/Giardia_intestinalis Giardia intestinalis]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4EVF OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:022796298</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Giardia intestinalis]]		[[Category: Giardia intestinalis]]
	[[Category: Hofmann, A.]]		[[Category: Hofmann, A]]
	[[Category: Weeratunga, S.]]		[[Category: Weeratunga, S]]
	[[Category: Annexin]]		[[Category: Annexin]]
	[[Category: Calcium-binding protein]]		[[Category: Calcium-binding protein]]
	[[Category: Membrane-binding protein]]		[[Category: Membrane-binding protein]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]

OCA at 03:59, 18 July 2013

2013-07-18T03:59:58Z

← Older revision		Revision as of 03:59, 18 July 2013
Line 1:		Line 1:
	~~[[Image:4evf.jpg\|left\|200px]]~~

	{{STRUCTURE_4evf\| PDB=4evf \| SCENE= }}		{{STRUCTURE_4evf\| PDB=4evf \| SCENE= }}

	===Crystal structure of apo alpha-1 giardin===		===Crystal structure of apo alpha-1 giardin===
			{{ABSTRACT_PUBMED_22796298}}

	==About this Structure==		==About this Structure==
	[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA].		[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA].

			==Reference==
			<ref group="xtra">PMID:022796298</ref><references group="xtra"/><references/>
	[[Category: Giardia intestinalis]]		[[Category: Giardia intestinalis]]
	[[Category: Hofmann, A.]]		[[Category: Hofmann, A.]]

OCA at 07:20, 25 July 2012

2012-07-25T07:20:26Z

← Older revision		Revision as of 07:20, 25 July 2012
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4evf.jpg\|left\|200px]]

	~~The entry~~ 4evf ~~is ON HOLD~~		{{STRUCTURE_4evf\| PDB=4evf \| SCENE= }}

	~~Authors: Weeratunga, S., Hofmann, A.~~		===Crystal structure of apo alpha-1 giardin===

	~~Description~~: ~~Crystal structure of apo alpha~~-~~1 giardin~~
			==About this Structure==
			[[4evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVF OCA].
			[[Category: Giardia intestinalis]]
			[[Category: Hofmann, A.]]
			[[Category: Weeratunga, S.]]
			[[Category: Annexin]]
			[[Category: Calcium-binding protein]]
			[[Category: Membrane-binding protein]]
			[[Category: Metal binding protein]]

OCA: Protected "4evf" [edit=sysop:move=sysop]

2012-05-02T19:00:54Z

Protected "4evf" [edit=sysop:move=sysop]

← Older revision	Revision as of 19:00, 2 May 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4evf is ON HOLD Authors: Weeratunga, S., Hofmann, A. Description: Crystal structure of apo alpha-1 giardin

2012-05-02T19:00:53Z

New page: '''Unreleased structure''' The entry 4evf is ON HOLD Authors: Weeratunga, S., Hofmann, A. Description: Crystal structure of apo alpha-1 giardin

New page

'''Unreleased structure'''

The entry 4evf is ON HOLD

Authors: Weeratunga, S., Hofmann, A.

Description: Crystal structure of apo alpha-1 giardin