4f8a - Revision history

OCA at 11:11, 1 March 2024

2024-03-01T11:11:34Z

← Older revision		Revision as of 11:11, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F8A FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [https://pdbe.org/4f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [https://www.ebi.ac.uk/pdbsum/4f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8a ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [https://pdbe.org/4f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [https://www.ebi.ac.uk/pdbsum/4f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/KCNH1_MOUSE KCNH1_MOUSE] Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts (By similarity).		[https://www.uniprot.org/uniprot/KCNH1_MOUSE KCNH1_MOUSE] Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts (By similarity).
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	KCNH channels are voltage-gated potassium channels with important physiological functions. In these channels, a C-terminal cytoplasmic region, known as the cyclic nucleotide binding homology (CNB-homology) domain displays strong sequence similarity to cyclic nucleotide binding (CNB) domains. However, the isolated domain does not bind cyclic nucleotides. Here, we report the X-ray structure of the CNB-homology domain from the mouse EAG1 channel. Through comparison with the recently determined structure of the CNB-homology domain from the zebrafish ELK (eag-like K(+)) channel and the CNB domains from the MlotiK1 and HCN (hyperpolarization-activated cyclic nucleotide-gated) potassium channels, we establish the structural features of CNB-homology domains that explain the low affinity for cyclic nucleotides. Our structure establishes that the "self-liganded" conformation, where two residues of the C-terminus of the domain are bound in an equivalent position to cyclic nucleotides in CNB domains, is a conserved feature of CNB-homology domains. Importantly, we provide biochemical evidence that suggests that there is also an unliganded conformation where the C-terminus of the domain peels away from its bound position. A functional characterization of this unliganded conformation reveals a role of the CNB-homology domain in channel gating.

	Structural, Biochemical, and Functional Characterization of the Cyclic Nucleotide Binding Homology Domain from the Mouse EAG1 Potassium Channel.,Marques-Carvalho MJ, Sahoo N, Muskett FW, Vieira-Pires RS, Gabant G, Cadene M, Schonherr R, Morais-Cabral JH J Mol Biol. 2012 Jun 23. PMID:22732247<ref>PMID:22732247</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4f8a" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:40, 7 October 2022

2022-10-07T04:40:36Z

← Older revision		Revision as of 04:40, 7 October 2022
Line 1:		Line 1:

	==Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel==		==Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel==
	<StructureSection load='4f8a' size='340' side='right' caption='[[4f8a]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4f8a' size='340' side='right'caption='[[4f8a]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f8a]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4F8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F8A FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Eag, Kcnh1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [https://pdbe.org/4f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [https://www.ebi.ac.uk/pdbsum/4f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8a ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [~~http~~://pdbe.org/4f8a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4f8a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/KCNH1_MOUSE KCNH1_MOUSE]] Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts (By similarity).		[https://www.uniprot.org/uniprot/KCNH1_MOUSE KCNH1_MOUSE] Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Large Structures]]
	[[Category: Cadene, M]]		[[Category: Mus musculus]]
	[[Category: Gabant, G]]		[[Category: Cadene M]]
	[[Category: Marques-Carvalho~~, M J~~]]		[[Category: Gabant G]]
	[[Category: Morais-Cabral~~, J H~~]]		[[Category: Marques-Carvalho MJ]]
	[[Category: Muskett~~, F W~~]]		[[Category: Morais-Cabral JH]]
	[[Category: Sahoo, N]]		[[Category: Muskett FW]]
	[[Category: Schonherr, R]]		[[Category: Sahoo N]]
	[[Category: Vieira-Pires~~, R S]]~~		[[Category: Schonherr R]]
	~~[[Category: Membrane protein]]~~		[[Category: Vieira-Pires RS]]
	~~[[Category: Probable regulatory domain of potassium channel]]~~
	~~[[Category: Transport protein~~]]

OCA at 02:34, 5 August 2016

2016-08-05T02:34:19Z

← Older revision		Revision as of 02:34, 5 August 2016
Line 1:		Line 1:

	==Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel==		==Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel==
	<StructureSection load='4f8a' size='340' side='right' caption='[[4f8a]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4f8a' size='340' side='right' caption='[[4f8a]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F8A FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Eag, Kcnh1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Eag, Kcnh1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [http://www.ebi.ac.uk/pdbsum/4f8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [http://pdbe.org/4f8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [http://www.ebi.ac.uk/pdbsum/4f8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4f8a" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Cadene, M]]		[[Category: Cadene, M]]
	[[Category: Gabant, G]]		[[Category: Gabant, G]]

OCA at 22:03, 24 December 2014

2014-12-24T22:03:41Z

← Older revision		Revision as of 22:03, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [http://www.ebi.ac.uk/pdbsum/4f8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [http://www.ebi.ac.uk/pdbsum/4f8a PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/KCNH1_MOUSE KCNH1_MOUSE]] Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:19, 9 December 2014

2014-12-09T14:19:56Z

← Older revision		Revision as of 14:19, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:4f8a.~~png\|left\|200px~~]]		==Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel==
			<StructureSection load='4f8a' size='340' side='right' caption='[[4f8a]], [[Resolution\|resolution]] 2.20Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4f8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F8A FirstGlance]. <br>
			</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Eag, Kcnh1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f8a RCSB], [http://www.ebi.ac.uk/pdbsum/4f8a PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			KCNH channels are voltage-gated potassium channels with important physiological functions. In these channels, a C-terminal cytoplasmic region, known as the cyclic nucleotide binding homology (CNB-homology) domain displays strong sequence similarity to cyclic nucleotide binding (CNB) domains. However, the isolated domain does not bind cyclic nucleotides. Here, we report the X-ray structure of the CNB-homology domain from the mouse EAG1 channel. Through comparison with the recently determined structure of the CNB-homology domain from the zebrafish ELK (eag-like K(+)) channel and the CNB domains from the MlotiK1 and HCN (hyperpolarization-activated cyclic nucleotide-gated) potassium channels, we establish the structural features of CNB-homology domains that explain the low affinity for cyclic nucleotides. Our structure establishes that the "self-liganded" conformation, where two residues of the C-terminus of the domain are bound in an equivalent position to cyclic nucleotides in CNB domains, is a conserved feature of CNB-homology domains. Importantly, we provide biochemical evidence that suggests that there is also an unliganded conformation where the C-terminus of the domain peels away from its bound position. A functional characterization of this unliganded conformation reveals a role of the CNB-homology domain in channel gating.

	~~{{STRUCTURE_4f8a\| PDB=4f8a \| SCENE= }}~~		Structural, Biochemical, and Functional Characterization of the Cyclic Nucleotide Binding Homology Domain from the Mouse EAG1 Potassium Channel.,Marques-Carvalho MJ, Sahoo N, Muskett FW, Vieira-Pires RS, Gabant G, Cadene M, Schonherr R, Morais-Cabral JH J Mol Biol. 2012 Jun 23. PMID:22732247<ref>PMID:22732247</ref>

	~~===Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22732247}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4f8a]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4F8A OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022732247<~~/~~ref><ref group="xtra">PMID:022442238</ref~~><~~references group="xtra"~~/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Cadene, M.]]		[[Category: Cadene, M]]
	[[Category: Gabant, G.]]		[[Category: Gabant, G]]
	[[Category: Marques-Carvalho, M J.]]		[[Category: Marques-Carvalho, M J]]
	[[Category: Morais-Cabral, J H.]]		[[Category: Morais-Cabral, J H]]
	[[Category: Muskett, F W.]]		[[Category: Muskett, F W]]
	[[Category: Sahoo, N.]]		[[Category: Sahoo, N]]
	[[Category: Schonherr, R.]]		[[Category: Schonherr, R]]
	[[Category: Vieira-Pires, R S.]]		[[Category: Vieira-Pires, R S]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Probable regulatory domain of potassium channel]]		[[Category: Probable regulatory domain of potassium channel]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 05:52, 17 October 2012

2012-10-17T05:52:47Z

← Older revision		Revision as of 05:52, 17 October 2012
Line 1:		Line 1:
	[[Image:4f8a.~~jpg~~\|left\|200px]]		[[Image:4f8a.png\|left\|200px]]

	{{STRUCTURE_4f8a\| PDB=4f8a \| SCENE= }}		{{STRUCTURE_4f8a\| PDB=4f8a \| SCENE= }}

OCA at 08:09, 11 July 2012

2012-07-11T08:09:31Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:4f8a.jpg|left|200px]]
-The entry 4f8a is ON HOLD  until Paper Publication
+{{STRUCTURE_4f8a|  PDB=4f8a  |  SCENE=  }}
-Authors: Marques-Carvalho, M.J., Sahoo, N., Muskett, F.W., Vieira-Pires, R.S., Gabant, G., Cadene, M., Schonherr, R., Morais-Cabral, J.H.
+===Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel===
-Description: Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel
+{{ABSTRACT_PUBMED_22732247}}

OCA at 07:23, 20 June 2012

2012-06-20T07:23:09Z

← Older revision		Revision as of 07:23, 20 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4f8a is ON HOLD		The entry 4f8a is ON HOLD until Paper Publication

	Authors: Marques-Carvalho, M.J., Sahoo, N., Muskett, F.W., Vieira-Pires, R.S., Gabant, G., Cadene, M., Schonherr, R., Morais-Cabral, J.H.		Authors: Marques-Carvalho, M.J., Sahoo, N., Muskett, F.W., Vieira-Pires, R.S., Gabant, G., Cadene, M., Schonherr, R., Morais-Cabral, J.H.

	Description: Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel		Description: Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel

OCA at 07:19, 6 June 2012

2012-06-06T07:19:56Z

← Older revision		Revision as of 07:19, 6 June 2012
Line 3:		Line 3:
	The entry 4f8a is ON HOLD		The entry 4f8a is ON HOLD

	Authors: Marques-Carvalho, M.J., Sahoo, N., Muskett, F.W., Vieira-Pires, R. S., Gabant, G., Cadene, M., Schonherr, R., Morais-Cabral, J.H.		Authors: Marques-Carvalho, M.J., Sahoo, N., Muskett, F.W., Vieira-Pires, R.S., Gabant, G., Cadene, M., Schonherr, R., Morais-Cabral, J.H.

	Description: Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel		Description: Cyclic nucleotide binding-homology domain from mouse EAG1 potassium channel

OCA: Protected "4f8a" [edit=sysop:move=sysop]

2012-05-23T05:45:36Z

Protected "4f8a" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:45, 23 May 2012
(No difference)