4fd2 - Revision history

OCA at 11:14, 1 March 2024

2024-03-01T11:14:19Z

← Older revision		Revision as of 11:14, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [https://pdbe.org/4fd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [https://www.ebi.ac.uk/pdbsum/4fd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fd2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [https://pdbe.org/4fd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [https://www.ebi.ac.uk/pdbsum/4fd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fd2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CLPB_THET8 CLPB_THET8] Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.<ref>PMID:10377389</ref>		[https://www.uniprot.org/uniprot/CLPB_THET8 CLPB_THET8] Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.<ref>PMID:10377389</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	ClpB is a ring-forming, ATP-dependent protein disaggregase that cooperates with the cognate Hsp70 system to recover functional protein from aggregates. How ClpB harnesses the energy of ATP binding and hydrolysis to facilitate the mechanical unfolding of previously aggregated, stress-damaged proteins remains unclear. Here, we present crystal structures of the ClpB D2 domain in the nucleotide-bound and -free states, and the fitted cryoEM structure of the D2 hexamer ring, which provide a structural understanding of the ATP power stroke that drives protein translocation through the ClpB hexamer. We demonstrate that the conformation of the substrate-translocating pore loop is coupled to the nucleotide state of the cis subunit, which is transmitted to the neighboring subunit via a conserved but structurally distinct intersubunit-signaling pathway common to diverse AAA+ machines. Furthermore, we found that an engineered, disulfide cross-linked ClpB hexamer is fully functional biochemically, suggesting that ClpB deoligomerization is not required for protein disaggregation.

	Structural basis for intersubunit signaling in a protein disaggregating machine.,Biter AB, Lee S, Sung N, Tsai FT Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12515-20. Epub 2012 Jul 16. PMID:22802670<ref>PMID:22802670</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4fd2" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==

OCA at 04:50, 7 October 2022

2022-10-07T04:50:11Z

← Older revision		Revision as of 04:50, 7 October 2022
Line 1:		Line 1:

	==Crystal structure of the C-terminal domain of ClpB==		==Crystal structure of the C-terminal domain of ClpB==
	<StructureSection load='4fd2' size='340' side='right' caption='[[4fd2]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4fd2' size='340' side='right'caption='[[4fd2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fct\|4fct]], [[4fcv\|4fcv]], [[4fcw\|4fcw]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [https://pdbe.org/4fd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [https://www.ebi.ac.uk/pdbsum/4fd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fd2 ProSAT]</span></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clpB, TTHA1487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [~~http~~://pdbe.org/4fd2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fd2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fd2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/CLPB_THET8 CLPB_THET8]] Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.<ref>PMID:10377389</ref>		[https://www.uniprot.org/uniprot/CLPB_THET8 CLPB_THET8] Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.<ref>PMID:10377389</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
	*[[Heat Shock ~~Proteins~~\|Heat Shock ~~Proteins~~]]		*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
			*[[3D structures of ClpB\|3D structures of ClpB]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thet8~~]]		[[Category: Large Structures]]
	[[Category: ~~Biter, A B~~]]		[[Category: Thermus thermophilus HB8]]
	[[Category: ~~Lee, S~~]]		[[Category: Biter AB]]
	[[Category: ~~Sung, N~~]]		[[Category: Lee S]]
	[[Category: ~~Tsai, F T.F~~]]		[[Category: Sung N]]
	[[Category: ~~Aaa domain]]~~		[[Category: Tsai FTF]]
	~~[[Category: Chaperone~~]]

OCA at 16:59, 5 August 2016

2016-08-05T16:59:24Z

← Older revision		Revision as of 16:59, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of the C-terminal domain of ClpB==		==Crystal structure of the C-terminal domain of ClpB==
	<StructureSection load='4fd2' size='340' side='right' caption='[[4fd2]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4fd2' size='340' side='right' caption='[[4fd2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fct\|4fct]], [[4fcv\|4fcv]], [[4fcw\|4fcw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fct\|4fct]], [[4fcv\|4fcv]], [[4fcw\|4fcw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clpB, TTHA1487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~274 Thermus thermophilus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clpB, TTHA1487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [http://www.ebi.ac.uk/pdbsum/4fd2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [http://pdbe.org/4fd2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [http://www.ebi.ac.uk/pdbsum/4fd2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fd2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4fd2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Heat Shock Proteins\|Heat Shock Proteins]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thermus thermophilus~~]]		[[Category: Thet8]]
	[[Category: Biter, A B]]		[[Category: Biter, A B]]
	[[Category: Lee, S]]		[[Category: Lee, S]]

OCA at 04:14, 25 December 2014

2014-12-25T04:14:05Z

← Older revision		Revision as of 04:14, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fct\|4fct]], [[4fcv\|4fcv]], [[4fcw\|4fcw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fct\|4fct]], [[4fcv\|4fcv]], [[4fcw\|4fcw]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clpB, TTHA1487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clpB, TTHA1487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [http://www.ebi.ac.uk/pdbsum/4fd2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [http://www.ebi.ac.uk/pdbsum/4fd2 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CLPB_THET8 CLPB_THET8]] Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.<ref>PMID:10377389</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Biter, A B.]]		[[Category: Biter, A B]]
	[[Category: Lee, S.]]		[[Category: Lee, S]]
	[[Category: Sung, N.]]		[[Category: Sung, N]]
	[[Category: Tsai, F T.F.]]		[[Category: Tsai, F T.F]]
	[[Category: Aaa domain]]		[[Category: Aaa domain]]
	[[Category: Chaperone]]		[[Category: Chaperone]]

OCA at 09:11, 11 June 2014

2014-06-11T09:11:24Z

← Older revision		Revision as of 09:11, 11 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~4fd2~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of the C-terminal domain of ClpB==
			<StructureSection load='4fd2' size='340' side='right' caption='[[4fd2]], [[Resolution\|resolution]] 3.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FD2 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fct\|4fct]], [[4fcv\|4fcv]], [[4fcw\|4fcw]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clpB, TTHA1487 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fd2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fd2 RCSB], [http://www.ebi.ac.uk/pdbsum/4fd2 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			ClpB is a ring-forming, ATP-dependent protein disaggregase that cooperates with the cognate Hsp70 system to recover functional protein from aggregates. How ClpB harnesses the energy of ATP binding and hydrolysis to facilitate the mechanical unfolding of previously aggregated, stress-damaged proteins remains unclear. Here, we present crystal structures of the ClpB D2 domain in the nucleotide-bound and -free states, and the fitted cryoEM structure of the D2 hexamer ring, which provide a structural understanding of the ATP power stroke that drives protein translocation through the ClpB hexamer. We demonstrate that the conformation of the substrate-translocating pore loop is coupled to the nucleotide state of the cis subunit, which is transmitted to the neighboring subunit via a conserved but structurally distinct intersubunit-signaling pathway common to diverse AAA+ machines. Furthermore, we found that an engineered, disulfide cross-linked ClpB hexamer is fully functional biochemically, suggesting that ClpB deoligomerization is not required for protein disaggregation.

	~~{{STRUCTURE_4fd2\| PDB=4fd2 \| SCENE= }}~~		Structural basis for intersubunit signaling in a protein disaggregating machine.,Biter AB, Lee S, Sung N, Tsai FT Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12515-20. Epub 2012 Jul 16. PMID:22802670<ref>PMID:22802670</ref>

	~~===Crystal structure~~ of the ~~C-terminal domain of ClpB===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22802670}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4fd2]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermus_thermophilus Thermus thermophilus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022802670<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Biter, A B.]]		[[Category: Biter, A B.]]

OCA at 05:10, 22 August 2012

2012-08-22T05:10:09Z

← Older revision		Revision as of 05:10, 22 August 2012
Line 1:		Line 1:
	[[Image:4fd2.~~jpg~~\|left\|200px]]		[[Image:4fd2.png\|left\|200px]]

	{{STRUCTURE_4fd2\| PDB=4fd2 \| SCENE= }}		{{STRUCTURE_4fd2\| PDB=4fd2 \| SCENE= }}
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	===Crystal structure of the C-terminal domain of ClpB===		===Crystal structure of the C-terminal domain of ClpB===

			{{ABSTRACT_PUBMED_22802670}}

	==About this Structure==		==About this Structure==
	[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA].		[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA].

			==Reference==
			<ref group="xtra">PMID:022802670</ref><references group="xtra"/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Biter, A B.]]		[[Category: Biter, A B.]]

OCA at 05:31, 18 July 2012

2012-07-18T05:31:27Z

← Older revision		Revision as of 05:31, 18 July 2012
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	~~'''Unreleased structure'''~~		[[Image:4fd2.jpg\|left\|200px]]

	~~The entry~~ 4fd2 ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4fd2\| PDB=4fd2 \| SCENE= }}

	~~Authors: Biter, A.B., Lee, S., Sung, N., Tsai, F.T.F.~~		===Crystal structure of the C-terminal domain of ClpB===

	~~Description~~: ~~Crystal structure of the C~~-~~terminal~~ domain ~~of ClpB~~
			==About this Structure==
			[[4fd2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FD2 OCA].
			[[Category: Thermus thermophilus]]
			[[Category: Biter, A B.]]
			[[Category: Lee, S.]]
			[[Category: Sung, N.]]
			[[Category: Tsai, F T.F.]]
			[[Category: Aaa domain]]
			[[Category: Chaperone]]

OCA at 05:23, 27 June 2012

2012-06-27T05:23:24Z

← Older revision		Revision as of 05:23, 27 June 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4fd2 is ON HOLD		The entry 4fd2 is ON HOLD until Paper Publication

	Authors: Biter, A.B., Lee, S., Sung, N., Tsai, F.T.F.		Authors: Biter, A.B., Lee, S., Sung, N., Tsai, F.T.F.

	Description: Crystal structure of the C-terminal domain of ClpB		Description: Crystal structure of the C-terminal domain of ClpB

OCA: Protected "4fd2" [edit=sysop:move=sysop]

2012-06-06T07:21:44Z

Protected "4fd2" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:21, 6 June 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4fd2 is ON HOLD Authors: Biter, A.B., Lee, S., Sung, N., Tsai, F.T.F. Description: Crystal structure of the C-terminal domain of ClpB

2012-06-06T07:21:43Z

New page: '''Unreleased structure''' The entry 4fd2 is ON HOLD Authors: Biter, A.B., Lee, S., Sung, N., Tsai, F.T.F. Description: Crystal structure of the C-terminal domain of ClpB

New page

'''Unreleased structure'''

The entry 4fd2 is ON HOLD

Authors: Biter, A.B., Lee, S., Sung, N., Tsai, F.T.F.

Description: Crystal structure of the C-terminal domain of ClpB