4ffa - Revision history

OCA at 10:51, 6 November 2024

2024-11-06T10:51:01Z

← Older revision		Revision as of 10:51, 6 November 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ATSK_MYCTU ATSK_MYCTU] Alpha-ketoglutarate-dependent sulfate ester dioxygenase, which oxidizes medium-chain alkyl-sulfate esters (PubMed:23762287). Shows preference for 2-ethylhexyl sulfate (2-EHS) in vitro, leading to the formation of succinate and 2-ethylhexanal (PubMed:23762287, PubMed:25427196). Has likely a role in sulfate scavenging in vivo (PubMed:23762287).<ref>PMID:23762287</ref> <ref>PMID:25427196</ref> Also causes the inactivation of the 2-carboxyquinoxaline Ty38c (an antitubercular compound that inhibits DprE1) via oxidative decarboxylation, using Ty38c instead of alpha-ketoglutarate as a substrate. Is thus responsible for primary resistance of M.tuberculosis to Ty38c in vitro. Overexpression of Rv3406 causes resistance to Ty38c.<ref>PMID:25427196</ref>		[https://www.uniprot.org/uniprot/ATSK_MYCTU ATSK_MYCTU] Alpha-ketoglutarate-dependent sulfate ester dioxygenase, which oxidizes medium-chain alkyl-sulfate esters (PubMed:23762287). Shows preference for 2-ethylhexyl sulfate (2-EHS) in vitro, leading to the formation of succinate and 2-ethylhexanal (PubMed:23762287, PubMed:25427196). Has likely a role in sulfate scavenging in vivo (PubMed:23762287).<ref>PMID:23762287</ref> <ref>PMID:25427196</ref> Also causes the inactivation of the 2-carboxyquinoxaline Ty38c (an antitubercular compound that inhibits DprE1) via oxidative decarboxylation, using Ty38c instead of alpha-ketoglutarate as a substrate. Is thus responsible for primary resistance of M.tuberculosis to Ty38c in vitro. Overexpression of Rv3406 causes resistance to Ty38c.<ref>PMID:25427196</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The genome of Mycobacterium tuberculosis (Mtb) encodes nine putative sulfatases, none of which have a known function or substrate. Here, we characterize Mtb's single putative type II sulfatase, Rv3406, as a non-heme iron (II) and alpha-ketoglutarate-dependent dioxygenase that catalyzes the oxidation and subsequent cleavage of alkyl sulfate esters. Rv3406 was identified based on its homology to the alkyl sulfatase AtsK from Pseudomonas putida. Using an in vitro biochemical assay, we confirmed that Rv3406 is a sulfatase with a preference for alkyl sulfate substrates similar to those processed by AtsK. We determined the crystal structure of the apo Rv3406 sulfatase at 2.5 A. The active site residues of Rv3406 and AtsK are essentially superimposable, suggesting that the two sulfatases share the same catalytic mechanism. Finally, we generated an Rv3406 mutant (Deltarv3406) in Mtb to study the sulfatase's role in sulfate scavenging. The Deltarv3406 strain did not replicate in minimal media with 2-ethyl hexyl sulfate as the sole sulfur source, in contrast to wild type Mtb or the complemented strain. We conclude that Rv3406 is an iron and alpha-ketoglutarate-dependent sulfate ester dioxygenase that has unique substrate specificity that is likely distinct from other Mtb sulfatases.

			Mycobacterium tuberculosis Rv3406 Is a Type II Alkyl Sulfatase Capable of Sulfate Scavenging.,Sogi KM, Gartner ZJ, Breidenbach MA, Appel MJ, Schelle MW, Bertozzi CR PLoS One. 2013 Jun 6;8(6):e65080. doi: 10.1371/journal.pone.0065080. Print 2013. PMID:23762287<ref>PMID:23762287</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4ffa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 15:23, 14 March 2024

2024-03-14T15:23:39Z

← Older revision		Revision as of 15:23, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ffa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ffa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ffa OCA], [https://pdbe.org/4ffa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ffa RCSB], [https://www.ebi.ac.uk/pdbsum/4ffa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ffa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ffa OCA], [https://pdbe.org/4ffa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ffa RCSB], [https://www.ebi.ac.uk/pdbsum/4ffa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ffa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ATSK_MYCTU ATSK_MYCTU] Alpha-ketoglutarate-dependent sulfate ester dioxygenase, which oxidizes medium-chain alkyl-sulfate esters (PubMed:23762287). Shows preference for 2-ethylhexyl sulfate (2-EHS) in vitro, leading to the formation of succinate and 2-ethylhexanal (PubMed:23762287, PubMed:25427196). Has likely a role in sulfate scavenging in vivo (PubMed:23762287).<ref>PMID:23762287</ref> <ref>PMID:25427196</ref> Also causes the inactivation of the 2-carboxyquinoxaline Ty38c (an antitubercular compound that inhibits DprE1) via oxidative decarboxylation, using Ty38c instead of alpha-ketoglutarate as a substrate. Is thus responsible for primary resistance of M.tuberculosis to Ty38c in vitro. Overexpression of Rv3406 causes resistance to Ty38c.<ref>PMID:25427196</ref>		[https://www.uniprot.org/uniprot/ATSK_MYCTU ATSK_MYCTU] Alpha-ketoglutarate-dependent sulfate ester dioxygenase, which oxidizes medium-chain alkyl-sulfate esters (PubMed:23762287). Shows preference for 2-ethylhexyl sulfate (2-EHS) in vitro, leading to the formation of succinate and 2-ethylhexanal (PubMed:23762287, PubMed:25427196). Has likely a role in sulfate scavenging in vivo (PubMed:23762287).<ref>PMID:23762287</ref> <ref>PMID:25427196</ref> Also causes the inactivation of the 2-carboxyquinoxaline Ty38c (an antitubercular compound that inhibits DprE1) via oxidative decarboxylation, using Ty38c instead of alpha-ketoglutarate as a substrate. Is thus responsible for primary resistance of M.tuberculosis to Ty38c in vitro. Overexpression of Rv3406 causes resistance to Ty38c.<ref>PMID:25427196</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The genome of Mycobacterium tuberculosis (Mtb) encodes nine putative sulfatases, none of which have a known function or substrate. Here, we characterize Mtb's single putative type II sulfatase, Rv3406, as a non-heme iron (II) and alpha-ketoglutarate-dependent dioxygenase that catalyzes the oxidation and subsequent cleavage of alkyl sulfate esters. Rv3406 was identified based on its homology to the alkyl sulfatase AtsK from Pseudomonas putida. Using an in vitro biochemical assay, we confirmed that Rv3406 is a sulfatase with a preference for alkyl sulfate substrates similar to those processed by AtsK. We determined the crystal structure of the apo Rv3406 sulfatase at 2.5 A. The active site residues of Rv3406 and AtsK are essentially superimposable, suggesting that the two sulfatases share the same catalytic mechanism. Finally, we generated an Rv3406 mutant (Deltarv3406) in Mtb to study the sulfatase's role in sulfate scavenging. The Deltarv3406 strain did not replicate in minimal media with 2-ethyl hexyl sulfate as the sole sulfur source, in contrast to wild type Mtb or the complemented strain. We conclude that Rv3406 is an iron and alpha-ketoglutarate-dependent sulfate ester dioxygenase that has unique substrate specificity that is likely distinct from other Mtb sulfatases.

	Mycobacterium tuberculosis Rv3406 Is a Type II Alkyl Sulfatase Capable of Sulfate Scavenging.,Sogi KM, Gartner ZJ, Breidenbach MA, Appel MJ, Schelle MW, Bertozzi CR PLoS One. 2013 Jun 6;8(6):e65080. doi: 10.1371/journal.pone.0065080. Print 2013. PMID:23762287<ref>PMID:23762287</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4ffa" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==

OCA at 04:53, 7 October 2022

2022-10-07T04:53:32Z

← Older revision		Revision as of 04:53, 7 October 2022
Line 1:		Line 1:

	==Sulfatase from Mycobacterium tuberculosis==		==Sulfatase from Mycobacterium tuberculosis==
	<StructureSection load='4ffa' size='340' side='right' caption='[[4ffa]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4ffa' size='340' side='right'caption='[[4ffa]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ffa]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus~~ tuberculosis~~" (zopf 1883) klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ffa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT3514, MTCY78.22c, Rv3406 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ffa OCA], [https://pdbe.org/4ffa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ffa RCSB], [https://www.ebi.ac.uk/pdbsum/4ffa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ffa ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ffa OCA], [~~http~~://pdbe.org/4ffa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ffa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ffa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ffa ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/ATSK_MYCTU ATSK_MYCTU] Alpha-ketoglutarate-dependent sulfate ester dioxygenase, which oxidizes medium-chain alkyl-sulfate esters (PubMed:23762287). Shows preference for 2-ethylhexyl sulfate (2-EHS) in vitro, leading to the formation of succinate and 2-ethylhexanal (PubMed:23762287, PubMed:25427196). Has likely a role in sulfate scavenging in vivo (PubMed:23762287).<ref>PMID:23762287</ref> <ref>PMID:25427196</ref> Also causes the inactivation of the 2-carboxyquinoxaline Ty38c (an antitubercular compound that inhibits DprE1) via oxidative decarboxylation, using Ty38c instead of alpha-ketoglutarate as a substrate. Is thus responsible for primary resistance of M.tuberculosis to Ty38c in vitro. Overexpression of Rv3406 causes resistance to Ty38c.<ref>PMID:25427196</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 20:

	==See Also==		==See Also==
	*[[Sulfatase\|Sulfatase]]		*[[Sulfatase 3D structures\|Sulfatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bertozzi, C R~~]]		[[Category: Large Structures]]
	[[Category: ~~Breidenbach, M A~~]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: ~~Gartner, Z J~~]]		[[Category: Bertozzi CR]]
	[[Category: ~~Sogi, K M~~]]		[[Category: Breidenbach MA]]
	[[Category: ~~Alkyl sulfatase~~]]		[[Category: Gartner ZJ]]
	[[Category: ~~Dioxygenase]]~~		[[Category: Sogi KM]]
	~~[[Category: Jelly roll]]~~
	~~[[Category: Oxidoreductase~~]]

OCA at 21:53, 4 August 2016

2016-08-04T21:53:52Z

← Older revision		Revision as of 21:53, 4 August 2016
Line 1:		Line 1:

	==Sulfatase from Mycobacterium tuberculosis==		==Sulfatase from Mycobacterium tuberculosis==
	<StructureSection load='4ffa' size='340' side='right' caption='[[4ffa]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4ffa' size='340' side='right' caption='[[4ffa]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ffa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium~~ tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ffa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT3514, MTCY78.22c, Rv3406 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 ~~Mycobacterium~~ tuberculosis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT3514, MTCY78.22c, Rv3406 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ffa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ffa RCSB], [http://www.ebi.ac.uk/pdbsum/4ffa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ffa OCA], [http://pdbe.org/4ffa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ffa RCSB], [http://www.ebi.ac.uk/pdbsum/4ffa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ffa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4ffa" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Sulfatase\|Sulfatase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Mycobacterium tuberculosis]]~~
	[[Category: Bertozzi, C R]]		[[Category: Bertozzi, C R]]
	[[Category: Breidenbach, M A]]		[[Category: Breidenbach, M A]]

OCA at 11:19, 21 December 2014

2014-12-21T11:19:51Z

← Older revision		Revision as of 11:19, 21 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4ffa~~\| ~~PDB~~=4ffa \| ~~SCENE~~= }}		==Sulfatase from Mycobacterium tuberculosis==
	===~~Sulfatase from~~ Mycobacterium tuberculosis===		<StructureSection load='4ffa' size='340' side='right' caption='[[4ffa]], [[Resolution\|resolution]] 2.50Å' scene=''>
	~~{{ABSTRACT_PUBMED_23762287}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4ffa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FFA FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MT3514, MTCY78.22c, Rv3406 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ffa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ffa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ffa RCSB], [http://www.ebi.ac.uk/pdbsum/4ffa PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The genome of Mycobacterium tuberculosis (Mtb) encodes nine putative sulfatases, none of which have a known function or substrate. Here, we characterize Mtb's single putative type II sulfatase, Rv3406, as a non-heme iron (II) and alpha-ketoglutarate-dependent dioxygenase that catalyzes the oxidation and subsequent cleavage of alkyl sulfate esters. Rv3406 was identified based on its homology to the alkyl sulfatase AtsK from Pseudomonas putida. Using an in vitro biochemical assay, we confirmed that Rv3406 is a sulfatase with a preference for alkyl sulfate substrates similar to those processed by AtsK. We determined the crystal structure of the apo Rv3406 sulfatase at 2.5 A. The active site residues of Rv3406 and AtsK are essentially superimposable, suggesting that the two sulfatases share the same catalytic mechanism. Finally, we generated an Rv3406 mutant (Deltarv3406) in Mtb to study the sulfatase's role in sulfate scavenging. The Deltarv3406 strain did not replicate in minimal media with 2-ethyl hexyl sulfate as the sole sulfur source, in contrast to wild type Mtb or the complemented strain. We conclude that Rv3406 is an iron and alpha-ketoglutarate-dependent sulfate ester dioxygenase that has unique substrate specificity that is likely distinct from other Mtb sulfatases.

	~~==About this Structure==~~		Mycobacterium tuberculosis Rv3406 Is a Type II Alkyl Sulfatase Capable of Sulfate Scavenging.,Sogi KM, Gartner ZJ, Breidenbach MA, Appel MJ, Schelle MW, Bertozzi CR PLoS One. 2013 Jun 6;8(6):e65080. doi: 10.1371/journal.pone.0065080. Print 2013. PMID:23762287<ref>PMID:23762287</ref>
	~~[[4ffa]] is~~ a ~~4 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki~~/~~Mycobacterium_tuberculosis Mycobacterium tuberculosis]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=4FFA OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:023762287</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Bertozzi, C R.]]		[[Category: Bertozzi, C R]]
	[[Category: Breidenbach, M A.]]		[[Category: Breidenbach, M A]]
	[[Category: Gartner, Z J.]]		[[Category: Gartner, Z J]]
	[[Category: Sogi, K M.]]		[[Category: Sogi, K M]]
	[[Category: Alkyl sulfatase]]		[[Category: Alkyl sulfatase]]
	[[Category: Dioxygenase]]		[[Category: Dioxygenase]]
	[[Category: Jelly roll]]		[[Category: Jelly roll]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 07:02, 30 June 2013

2013-06-30T07:02:35Z

← Older revision		Revision as of 07:02, 30 June 2013
Line 1:		Line 1:
	{{STRUCTURE_4ffa\| PDB=4ffa \| SCENE= }}		{{STRUCTURE_4ffa\| PDB=4ffa \| SCENE= }}
	===Sulfatase from Mycobacterium tuberculosis===		===Sulfatase from Mycobacterium tuberculosis===
			{{ABSTRACT_PUBMED_23762287}}

	==About this Structure==		==About this Structure==
	[[4ffa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA].		[[4ffa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA].

			==Reference==
			<ref group="xtra">PMID:023762287</ref><references group="xtra"/><references/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Bertozzi, C R.]]		[[Category: Bertozzi, C R.]]

OCA at 18:12, 19 June 2013

2013-06-19T18:12:58Z

← Older revision		Revision as of 18:12, 19 June 2013
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	~~'''Unreleased structure'''~~		{{STRUCTURE_4ffa\| PDB=4ffa \| SCENE= }}
			===Sulfatase from Mycobacterium tuberculosis===

	~~The entry~~ 4ffa is ~~ON HOLD until Paper Publication~~		==About this Structure==
			[[4ffa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FFA OCA].
	~~Authors~~: ~~Sogi~~, K.M., Gartner, Z.J., ~~Breidenbach,~~ M.~~A., Bertozzi, C.R.~~		[[Category: Mycobacterium tuberculosis]]
			[[Category: Bertozzi, C R.]]
	~~Description~~: ~~Sulfatase from Mycobacterium tuberculosis~~		[[Category: Breidenbach, M A.]]
			[[Category: Gartner, Z J.]]
			[[Category: Sogi, K M.]]
			[[Category: Alkyl sulfatase]]
			[[Category: Dioxygenase]]
			[[Category: Jelly roll]]
			[[Category: Oxidoreductase]]

OCA at 05:23, 27 June 2012

2012-06-27T05:23:50Z

← Older revision		Revision as of 05:23, 27 June 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4ffa is ON HOLD		The entry 4ffa is ON HOLD until Paper Publication

	Authors: Sogi, K.M., Gartner, Z.J., Breidenbach, M.A., Bertozzi, C.R.		Authors: Sogi, K.M., Gartner, Z.J., Breidenbach, M.A., Bertozzi, C.R.

	Description: Sulfatase from Mycobacterium tuberculosis		Description: Sulfatase from Mycobacterium tuberculosis

OCA: Protected "4ffa" [edit=sysop:move=sysop]

2012-06-06T07:23:56Z

Protected "4ffa" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:23, 6 June 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4ffa is ON HOLD Authors: Sogi, K.M., Gartner, Z.J., Breidenbach, M.A., Bertozzi, C.R. Description: Sulfatase from Mycobacterium tuberculosis

2012-06-06T07:23:55Z

New page: '''Unreleased structure''' The entry 4ffa is ON HOLD Authors: Sogi, K.M., Gartner, Z.J., Breidenbach, M.A., Bertozzi, C.R. Description: Sulfatase from Mycobacterium tuberculosis

New page

'''Unreleased structure'''

The entry 4ffa is ON HOLD

Authors: Sogi, K.M., Gartner, Z.J., Breidenbach, M.A., Bertozzi, C.R.

Description: Sulfatase from Mycobacterium tuberculosis