4fix - Revision history

OCA at 11:16, 1 March 2024

2024-03-01T11:16:53Z

← Older revision		Revision as of 11:16, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FIX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [https://pdbe.org/4fix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [https://www.ebi.ac.uk/pdbsum/4fix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fix ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [https://pdbe.org/4fix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [https://www.ebi.ac.uk/pdbsum/4fix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GLFT2_MYCTU GLFT2_MYCTU] Involved in the polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacteria cell wall, through successively beta-D-(1->5) and beta-D-(1->6)-galactofuranosyltransferases activities. It transfers the galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55), yielding polygalactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl ((Galf)x-Rha-GlcNAc-PP-C55). It is able to transfer Galf onto beta-D-(1->5) or beta-D-(1->6) linked acceptor, but has a stronger affinity for beta-D-(1->6) acceptor.<ref>PMID:10934214</ref> <ref>PMID:11304545</ref> <ref>PMID:16704275</ref> <ref>PMID:18055597</ref> <ref>PMID:18423586</ref> <ref>PMID:19571009</ref>		[https://www.uniprot.org/uniprot/GLFT2_MYCTU GLFT2_MYCTU] Involved in the polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacteria cell wall, through successively beta-D-(1->5) and beta-D-(1->6)-galactofuranosyltransferases activities. It transfers the galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55), yielding polygalactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl ((Galf)x-Rha-GlcNAc-PP-C55). It is able to transfer Galf onto beta-D-(1->5) or beta-D-(1->6) linked acceptor, but has a stronger affinity for beta-D-(1->6) acceptor.<ref>PMID:10934214</ref> <ref>PMID:11304545</ref> <ref>PMID:16704275</ref> <ref>PMID:18055597</ref> <ref>PMID:18423586</ref> <ref>PMID:19571009</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Biosynthesis of the mycobacterial cell wall relies on the activities of many enzymes, including several glycosyltransferases (GTs). The polymerizing galactofuranosyltransferase GlfT2 (Rv3808c) synthesizes the bulk of the galactan portion of the mycolyl-arabinogalactan complex, which is the largest component of the mycobacterial cell wall. We used x-ray crystallography to determine the 2.45-A resolution crystal structure of GlfT2, revealing an unprecedented multidomain structure in which an N-terminal beta-barrel domain and two primarily alpha-helical C-terminal domains flank a central GT-A domain. The kidney-shaped protomers assemble into a C(4)-symmetric homotetramer with an open central core and a surface containing exposed hydrophobic and positively charged residues likely involved with membrane binding. The structure of a 3.1-A resolution complex of GlfT2 with UDP reveals a distinctive mode of nucleotide recognition. In addition, models for the binding of UDP-galactofuranose and acceptor substrates in combination with site-directed mutagenesis and kinetic studies suggest a mechanism that explains the unique ability of GlfT2 to generate alternating beta-(1-->5) and beta-(1-->6) glycosidic linkages using a single active site. The topology imposed by docking a tetrameric assembly onto a membrane bilayer also provides novel insights into aspects of processivity and chain length regulation in this and possibly other polymerizing GTs.

	Tetrameric Structure of the GlfT2 Galactofuranosyltransferase Reveals a Scaffold for the Assembly of Mycobacterial Arabinogalactan.,Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK J Biol Chem. 2012 Aug 10;287(33):28132-43. Epub 2012 Jun 15. PMID:22707726<ref>PMID:22707726</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4fix" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:51, 19 October 2022

2022-10-19T19:51:13Z

← Older revision		Revision as of 19:51, 19 October 2022
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	==Crystal Structure of GlfT2==		==Crystal Structure of GlfT2==
	<StructureSection load='4fix' size='340' side='right' caption='[[4fix]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='4fix' size='340' side='right'caption='[[4fix]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fix]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FIX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fiy\|4fiy]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [https://pdbe.org/4fix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [https://www.ebi.ac.uk/pdbsum/4fix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fix ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glfT2, Rv3808c, RV38308c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [~~http~~://pdbe.org/4fix PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fix PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/GLFT2_MYCTU GLFT2_MYCTU]] Involved in the polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacteria cell wall, through successively beta-D-(1->5) and beta-D-(1->6)-galactofuranosyltransferases activities. It transfers the galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55), yielding polygalactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl ((Galf)x-Rha-GlcNAc-PP-C55). It is able to transfer Galf onto beta-D-(1->5) or beta-D-(1->6) linked acceptor, but has a stronger affinity for beta-D-(1->6) acceptor.<ref>PMID:10934214</ref> <ref>PMID:11304545</ref> <ref>PMID:16704275</ref> <ref>PMID:18055597</ref> <ref>PMID:18423586</ref> <ref>PMID:19571009</ref>		[https://www.uniprot.org/uniprot/GLFT2_MYCTU GLFT2_MYCTU] Involved in the polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacteria cell wall, through successively beta-D-(1->5) and beta-D-(1->6)-galactofuranosyltransferases activities. It transfers the galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55), yielding polygalactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl ((Galf)x-Rha-GlcNAc-PP-C55). It is able to transfer Galf onto beta-D-(1->5) or beta-D-(1->6) linked acceptor, but has a stronger affinity for beta-D-(1->6) acceptor.<ref>PMID:10934214</ref> <ref>PMID:11304545</ref> <ref>PMID:16704275</ref> <ref>PMID:18055597</ref> <ref>PMID:18423586</ref> <ref>PMID:19571009</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Myctu~~]]		[[Category: Large Structures]]
	[[Category: ~~Lowary, T L~~]]		[[Category: Mycobacterium tuberculosis H37Rv]]
	[[Category: ~~Ng, K K.S~~]]		[[Category: Lowary TL]]
	[[Category: ~~Wheatley, R W~~]]		[[Category: Ng KKS]]
	[[Category: ~~Zheng, R B~~]]		[[Category: Wheatley RW]]
	[[Category: ~~Carbohydrate binding]]~~		[[Category: Zheng RB]]
	~~[[Category: Cazy gt-2 family]]~~
	~~[[Category: Galactofuranosyltransferase]]~~
	~~[[Category: Glycosyltransferase]]~~
	~~[[Category: Membrane]]~~
	~~[[Category: Transferase~~]]

OCA at 11:21, 5 August 2016

2016-08-05T11:21:10Z

← Older revision		Revision as of 11:21, 5 August 2016
Line 1:		Line 1:

	==Crystal Structure of GlfT2==		==Crystal Structure of GlfT2==
	<StructureSection load='4fix' size='340' side='right' caption='[[4fix]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='4fix' size='340' side='right' caption='[[4fix]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FIX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fiy\|4fiy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fiy\|4fiy]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glfT2, Rv3808c, RV38308c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 ~~Mycobacterium tuberculosis H37Rv~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glfT2, Rv3808c, RV38308c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [http://www.ebi.ac.uk/pdbsum/4fix PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [http://pdbe.org/4fix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [http://www.ebi.ac.uk/pdbsum/4fix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4fix" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mycobacterium tuberculosis h37rv~~]]		[[Category: Myctu]]
	[[Category: Lowary, T L]]		[[Category: Lowary, T L]]
	[[Category: Ng, K K.S]]		[[Category: Ng, K K.S]]

OCA at 01:58, 25 December 2014

2014-12-25T01:58:51Z

← Older revision		Revision as of 01:58, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [http://www.ebi.ac.uk/pdbsum/4fix PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [http://www.ebi.ac.uk/pdbsum/4fix PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GLFT2_MYCTU GLFT2_MYCTU]] Involved in the polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacteria cell wall, through successively beta-D-(1->5) and beta-D-(1->6)-galactofuranosyltransferases activities. It transfers the galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55), yielding polygalactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl ((Galf)x-Rha-GlcNAc-PP-C55). It is able to transfer Galf onto beta-D-(1->5) or beta-D-(1->6) linked acceptor, but has a stronger affinity for beta-D-(1->6) acceptor.<ref>PMID:10934214</ref> <ref>PMID:11304545</ref> <ref>PMID:16704275</ref> <ref>PMID:18055597</ref> <ref>PMID:18423586</ref> <ref>PMID:19571009</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:47, 9 December 2014

2014-12-09T15:47:40Z

← Older revision		Revision as of 15:47, 9 December 2014
Line 1:		Line 1:
	[[~~Image~~:~~4fix~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of GlfT2==
			<StructureSection load='4fix' size='340' side='right' caption='[[4fix]], [[Resolution\|resolution]] 2.45Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FIX FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fiy\|4fiy]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glfT2, Rv3808c, RV38308c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium tuberculosis H37Rv])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fix RCSB], [http://www.ebi.ac.uk/pdbsum/4fix PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Biosynthesis of the mycobacterial cell wall relies on the activities of many enzymes, including several glycosyltransferases (GTs). The polymerizing galactofuranosyltransferase GlfT2 (Rv3808c) synthesizes the bulk of the galactan portion of the mycolyl-arabinogalactan complex, which is the largest component of the mycobacterial cell wall. We used x-ray crystallography to determine the 2.45-A resolution crystal structure of GlfT2, revealing an unprecedented multidomain structure in which an N-terminal beta-barrel domain and two primarily alpha-helical C-terminal domains flank a central GT-A domain. The kidney-shaped protomers assemble into a C(4)-symmetric homotetramer with an open central core and a surface containing exposed hydrophobic and positively charged residues likely involved with membrane binding. The structure of a 3.1-A resolution complex of GlfT2 with UDP reveals a distinctive mode of nucleotide recognition. In addition, models for the binding of UDP-galactofuranose and acceptor substrates in combination with site-directed mutagenesis and kinetic studies suggest a mechanism that explains the unique ability of GlfT2 to generate alternating beta-(1-->5) and beta-(1-->6) glycosidic linkages using a single active site. The topology imposed by docking a tetrameric assembly onto a membrane bilayer also provides novel insights into aspects of processivity and chain length regulation in this and possibly other polymerizing GTs.

	~~{{STRUCTURE_4fix\| PDB=4fix \| SCENE= }}~~		Tetrameric Structure of the GlfT2 Galactofuranosyltransferase Reveals a Scaffold for the Assembly of Mycobacterial Arabinogalactan.,Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK J Biol Chem. 2012 Aug 10;287(33):28132-43. Epub 2012 Jun 15. PMID:22707726<ref>PMID:22707726</ref>

	~~===Crystal Structure~~ of ~~GlfT2===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22707726}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[4fix]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:022707726<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mycobacterium tuberculosis h37rv]]		[[Category: Mycobacterium tuberculosis h37rv]]
	[[Category: Lowary, T L.]]		[[Category: Lowary, T L]]
	[[Category: Ng, K K.S.]]		[[Category: Ng, K K.S]]
	[[Category: Wheatley, R W.]]		[[Category: Wheatley, R W]]
	[[Category: Zheng, R B.]]		[[Category: Zheng, R B]]
	[[Category: Carbohydrate binding]]		[[Category: Carbohydrate binding]]
	[[Category: Cazy gt-2 family]]		[[Category: Cazy gt-2 family]]

OCA at 06:07, 17 October 2012

2012-10-17T06:07:39Z

← Older revision		Revision as of 06:07, 17 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis~~ Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA].		[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:022707726</ref><references group="xtra"/>		<ref group="xtra">PMID:022707726</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis h37rv]]
	[[Category: Lowary, T L.]]		[[Category: Lowary, T L.]]
	[[Category: Ng, K K.S.]]		[[Category: Ng, K K.S.]]

OCA at 05:11, 29 August 2012

2012-08-29T05:11:26Z

← Older revision		Revision as of 05:11, 29 August 2012
Line 1:		Line 1:
	[[Image:4fix.~~jpg~~\|left\|200px]]		[[Image:4fix.png\|left\|200px]]

	{{STRUCTURE_4fix\| PDB=4fix \| SCENE= }}		{{STRUCTURE_4fix\| PDB=4fix \| SCENE= }}
Line 5:		Line 5:
	===Crystal Structure of GlfT2===		===Crystal Structure of GlfT2===

			{{ABSTRACT_PUBMED_22707726}}

	==About this Structure==		==About this Structure==
	[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA].		[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA].

			==Reference==
			<ref group="xtra">PMID:022707726</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Lowary, T L.]]		[[Category: Lowary, T L.]]

OCA: Protected "4fix" [edit=sysop:move=sysop]

2012-06-20T08:02:26Z

Protected "4fix" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:02, 20 June 2012
(No difference)

OCA: New page: 200px {{STRUCTURE_4fix| PDB=4fix | SCENE= }} ===Crystal Structure of GlfT2=== ==About this Structure== 4fix is a 2 chain structure with sequence from [...

2012-06-20T08:02:23Z

New page: left|200px {{STRUCTURE_4fix| PDB=4fix | SCENE= }} ===Crystal Structure of GlfT2=== ==About this Structure== 4fix is a 2 chain structure with sequence from [...

New page

[[Image:4fix.jpg|left|200px]]

{{STRUCTURE_4fix| PDB=4fix | SCENE= }}

===Crystal Structure of GlfT2===

==About this Structure==
[[4fix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIX OCA].
[[Category: Mycobacterium tuberculosis]]
[[Category: Lowary, T L.]]
[[Category: Ng, K K.S.]]
[[Category: Wheatley, R W.]]
[[Category: Zheng, R B.]]
[[Category: Carbohydrate binding]]
[[Category: Cazy gt-2 family]]
[[Category: Galactofuranosyltransferase]]
[[Category: Glycosyltransferase]]
[[Category: Membrane]]
[[Category: Transferase]]