4fvb - Revision history

OCA at 13:52, 8 November 2023

2023-11-08T13:52:45Z

← Older revision		Revision as of 13:52, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FVB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvb OCA], [https://pdbe.org/4fvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fvb RCSB], [https://www.ebi.ac.uk/pdbsum/4fvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fvb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvb OCA], [https://pdbe.org/4fvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fvb RCSB], [https://www.ebi.ac.uk/pdbsum/4fvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fvb ProSAT]</span></td></tr>
	</table>		</table>

OCA at 20:12, 19 October 2022

2022-10-19T20:12:06Z

← Older revision		Revision as of 20:12, 19 October 2022
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	<StructureSection load='4fvb' size='340' side='right'caption='[[4fvb]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4fvb' size='340' side='right'caption='[[4fvb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ev-71 Ev-71~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FVB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] </span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvb OCA], [https://pdbe.org/4fvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fvb RCSB], [https://www.ebi.ac.uk/pdbsum/4fvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fvb ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvb OCA], [~~http~~://pdbe.org/4fvb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fvb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fvb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/~~A9XG43_9ENTO A9XG43_9ENTO~~]] ~~Protein 2C associates~~ with and induces ~~structural rearrangements~~ of ~~intracellular membranes~~. ~~It displays~~ RNA-~~binding~~, ~~nucleotide binding~~ and ~~NTPase activities~~ (By similarity).[~~SAAS~~:~~SAAS000199_004_016611~~] ~~Protein 3C~~ is a ~~cysteine~~ protease that ~~generates mature~~ viral proteins ~~from~~ the ~~precursor~~ polyprotein. ~~In addition~~ to ~~its proteolytic activity~~, ~~it binds~~ to viral ~~RNA~~, ~~and thus influences~~ viral genome replication. RNA and ~~substrate bind co~~-~~operatively~~ to the ~~protease~~ (~~By similarity~~).[~~SAAS~~:~~SAAS000199_004_042266~~] RNA-directed RNA polymerase 3D-~~POL replicates~~ genomic and ~~antigenomic~~ RNA ~~by recognizing replications specific signals~~ (~~By similarity~~).[~~SAAS~~:~~SAAS000199_004_010047~~]		[https://www.uniprot.org/uniprot/A9XG43_HE71 A9XG43_HE71] Acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU (By similarity). The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome (By similarity). Following genome release from the infecting virion in the cytoplasm, the VPg-RNA linkage is probably removed by host TDP2 (By similarity). During the late stage of the replication cycle, host TDP2 is excluded from sites of viral RNA synthesis and encapsidation, allowing for the generation of progeny virions.[ARBA:ARBA00024846] Capsid protein VP0: Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation. Allows the capsid to remain inactive before the maturation step.[RuleBase:RU364118] Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks.[RuleBase:RU364118] Capsid protein VP2: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[RuleBase:RU364118] Capsid protein VP3: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[RuleBase:RU364118] Capsid protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm.[RuleBase:RU364118] Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation (By similarity). Allows the capsid to remain inactive before the maturation step.[ARBA:ARBA00025202] Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3 (By similarity). The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome.[ARBA:ARBA00025306] Lies on the inner surface of the capsid shell (By similarity). After binding to the host receptor, the capsid undergoes conformational changes (By similarity). Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm.[ARBA:ARBA00024643] Protease 2A: Cysteine protease that cleaves viral polyprotein and specific host proteins.[RuleBase:RU364118] Protease 3C: Major viral protease that mediates proteolytic processing of the polyprotein. Cleaves host EIF5B, contributing to host translation shutoff. Cleaves also host PABPC1, contributing to host translation shutoff.[RuleBase:RU364118] Protein 2B: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cytoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication.[RuleBase:RU364118] Protein 2C: Induces and associates with structural rearrangements of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3.[RuleBase:RU364118] Protein 3A: Localizes the viral replication complex to the surface of membranous vesicles. It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the disassembly of the Golgi complex, possibly through GBF1 interaction. This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface.[RuleBase:RU364118] Protein 3AB: Localizes the viral replication complex to the surface of membranous vesicles. Together with protein 3CD binds the Cis-Active RNA Element (CRE) which is involved in RNA synthesis initiation. Acts as a cofactor to stimulate the activity of 3D polymerase, maybe through a nucleid acid chaperone activity.[RuleBase:RU364118] Protein 3CD: Involved in the viral replication complex and viral polypeptide maturation. It exhibits protease activity with a specificity and catalytic efficiency that is different from protease 3C. Protein 3CD lacks polymerase activity. Protein 3CD binds to the 5'UTR of the viral genome.[RuleBase:RU364118] RNA-directed RNA polymerase: Replicates the viral genomic RNA on the surface of intracellular membranes. May form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated.[RuleBase:RU364118] Viral protein genome-linked: acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome.[RuleBase:RU364118]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Proteinase\|Proteinase]]		*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Ev-71~~]]		[[Category: Enterovirus A71]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Picornain 2A]]~~		[[Category: Cai Q]]
	[[Category: Cai, Q]]		[[Category: Cai Y]]
	[[Category: Cai, Y]]		[[Category: Gao Z]]
	[[Category: Gao, Z]]		[[Category: Li J]]
	[[Category: Li, J]]		[[Category: Lin T]]
	[[Category: Lin, T]]		[[Category: Liu W]]
	[[Category: Liu, W]]		[[Category: Muhammad Y]]
	[[Category: Muhammad, Y]]		[[Category: Peng X]]
	[[Category: Peng, X]]		[[Category: Wu C]]
	[[Category: Wu, C]]		[[Category: Zheng Q]]
	[[Category: Zheng, Q]]
	~~[[Category: Cysteine proteinase]]~~
	~~[[Category: Hydrolase~~]]

OCA at 07:33, 27 March 2019

2019-03-27T07:33:35Z

← Older revision		Revision as of 07:33, 27 March 2019
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	==Crystal structure of EV71 2A proteinase C110A mutant==		==Crystal structure of EV71 2A proteinase C110A mutant==
	<StructureSection load='4fvb' size='340' side='right' caption='[[4fvb]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4fvb' size='340' side='right'caption='[[4fvb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FVB FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Ev-71]]		[[Category: Ev-71]]
			[[Category: Large Structures]]
	[[Category: Picornain 2A]]		[[Category: Picornain 2A]]
	[[Category: Cai, Q]]		[[Category: Cai, Q]]

OCA at 02:51, 5 August 2016

2016-08-05T02:51:45Z

← Older revision		Revision as of 02:51, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of EV71 2A proteinase C110A mutant==		==Crystal structure of EV71 2A proteinase C110A mutant==
	<StructureSection load='4fvb' size='340' side='right' caption='[[4fvb]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4fvb' size='340' side='right' caption='[[4fvb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterovirus_a71 Enterovirus a71~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ev-71 Ev-71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FVB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fvb RCSB], [http://www.ebi.ac.uk/pdbsum/4fvb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvb OCA], [http://pdbe.org/4fvb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fvb RCSB], [http://www.ebi.ac.uk/pdbsum/4fvb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4fvb" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Proteinase\|Proteinase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Enterovirus a71~~]]		[[Category: Ev-71]]
	[[Category: Picornain 2A]]		[[Category: Picornain 2A]]
	[[Category: Cai, Q]]		[[Category: Cai, Q]]

OCA at 22:12, 24 December 2014

2014-12-24T22:12:16Z

← Older revision		Revision as of 22:12, 24 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4fvb\| PDB=4fvb \| SCENE= }}~~		==Crystal structure of EV71 2A proteinase C110A mutant==
	===Crystal structure of EV71 2A proteinase C110A mutant===		<StructureSection load='4fvb' size='340' side='right' caption='[[4fvb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	~~{{ABSTRACT_PUBMED_23616646}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4fvb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterovirus_a71 Enterovirus a71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FVB FirstGlance]. <br>
	==Function==		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fvb RCSB], [http://www.ebi.ac.uk/pdbsum/4fvb PDBsum]</span></td></tr>
			</table>
			== Function ==
	[[http://www.uniprot.org/uniprot/A9XG43_9ENTO A9XG43_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]		[[http://www.uniprot.org/uniprot/A9XG43_9ENTO A9XG43_9ENTO]] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			2A proteinase (2Apro) is an enterovirally encoded cysteine protease that plays essential roles in both the processing of viral precursor polyprotein and the hijack of host cell translation and other processes in the virus life cycle. The crystallographic studies of 2Apro from enterovirus 71 and its interaction with the substrate are reported here. EV71 2Apro was comprised of an N-terminal domain of four-stranded antiparallel beta-sheet and a C-terminal domain of six-stranded antiparallel beta-barrel with a tightly bound zinc atom. Unlike other 2Apro structures, there is an open cleft across the surface of the protein in an open conformation. As demonstrated by the crystallographic studies and modeling of the complex structure, the open cleft could be fitted with the substrate. On comparison 2Apro of EV71 to those of the human rhinovirus 2 and coxsackievirus B4, the open conformation could be closed with a hinge motion in the bII2 and cII beta-strands. This was supported by molecular dynamic simulation. The structural variation among different 2Apro structures implicates a conformational flexibility in the substrate-binding cleft. The open structure provides an accessible framework for the design and development of therapeutics against the viral target.

	~~==About this Structure==~~		Conformational Plasticity of 2A Proteinase from Enterovirus 71.,Cai Q, Yameen M, Liu W, Gao Z, Li Y, Peng X, Cai Y, Wu C, Zheng Q, Li J, Lin T J Virol. 2013 Apr 24. PMID:23616646<ref>PMID:23616646</ref>
	~~[[4fvb]] is a 1 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Enterovirus_a71 Enterovirus a71]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4FVB OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:023616646</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Enterovirus a71]]		[[Category: Enterovirus a71]]
	[[Category: Picornain 2A]]		[[Category: Picornain 2A]]
	[[Category: Cai, Q.]]		[[Category: Cai, Q]]
	[[Category: Cai, Y.]]		[[Category: Cai, Y]]
	[[Category: Gao, Z.]]		[[Category: Gao, Z]]
	[[Category: Li, J.]]		[[Category: Li, J]]
	[[Category: Lin, T.]]		[[Category: Lin, T]]
	[[Category: Liu, W.]]		[[Category: Liu, W]]
	[[Category: Muhammad, Y.]]		[[Category: Muhammad, Y]]
	[[Category: Peng, X.]]		[[Category: Peng, X]]
	[[Category: Wu, C.]]		[[Category: Wu, C]]
	[[Category: Zheng, Q.]]		[[Category: Zheng, Q]]
	[[Category: Cysteine proteinase]]		[[Category: Cysteine proteinase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 13:49, 19 June 2013

2013-06-19T13:49:21Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{STRUCTURE_4fvb|  PDB=4fvb  |  SCENE=  }}
-The entry 4fvb is ON HOLD  until Paper Publication
+==Function==
-Authors: Cai, Q., Muhammad, Y., Liu, W., Gao, Z., Peng, X., Cai, Y., Wu, C., Zheng, Q., Li, J., Lin, T.
+==About this Structure==
-Description: Crystal structure of EV71 2A proteinase C110A mutant
+==Reference==

OCA at 05:04, 18 July 2012

2012-07-18T05:04:37Z

← Older revision		Revision as of 05:04, 18 July 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4fvb is ON HOLD		The entry 4fvb is ON HOLD until Paper Publication

	Authors: Cai, Q., Muhammad, Y., Liu, W., Gao, Z., Peng, X., Cai, Y., Wu, C., Zheng, Q., Li, J., Lin, T.		Authors: Cai, Q., Muhammad, Y., Liu, W., Gao, Z., Peng, X., Cai, Y., Wu, C., Zheng, Q., Li, J., Lin, T.

	Description: Crystal structure of EV71 2A proteinase C110A mutant		Description: Crystal structure of EV71 2A proteinase C110A mutant

OCA at 07:50, 11 July 2012

2012-07-11T07:50:35Z

← Older revision		Revision as of 07:50, 11 July 2012
Line 3:		Line 3:
	The entry 4fvb is ON HOLD		The entry 4fvb is ON HOLD

	Authors: Cai, ~~Qixu~~, Muhammad, ~~Yameen~~, Liu, ~~Weihua~~, Gao, ~~Zhenting~~, Peng, ~~Xuanjia~~, Cai, ~~Yaxian~~, Wu, ~~Caiming~~, Zheng, ~~Qian~~, Li, ~~Jian~~, Lin, ~~Tianwei~~		Authors: Cai, Q., Muhammad, Y., Liu, W., Gao, Z., Peng, X., Cai, Y., Wu, C., Zheng, Q., Li, J., Lin, T.

	Description: Crystal structure of EV71 2A proteinase C110A mutant		Description: Crystal structure of EV71 2A proteinase C110A mutant

OCA: Protected "4fvb" [edit=sysop:move=sysop]

2012-07-04T07:24:23Z

Protected "4fvb" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:24, 4 July 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4fvb is ON HOLD Authors: Cai, Qixu, Muhammad, Yameen, Liu, Weihua, Gao, Zhenting, Peng, Xuanjia, Cai, Yaxian, Wu, Caiming, Zheng, Qian, Li, Jian, Li...

2012-07-04T07:24:22Z

New page: '''Unreleased structure''' The entry 4fvb is ON HOLD Authors: Cai, Qixu, Muhammad, Yameen, Liu, Weihua, Gao, Zhenting, Peng, Xuanjia, Cai, Yaxian, Wu, Caiming, Zheng, Qian, Li, Jian, Li...

New page

'''Unreleased structure'''

The entry 4fvb is ON HOLD

Authors: Cai, Qixu, Muhammad, Yameen, Liu, Weihua, Gao, Zhenting, Peng, Xuanjia, Cai, Yaxian, Wu, Caiming, Zheng, Qian, Li, Jian, Lin, Tianwei

Description: Crystal structure of EV71 2A proteinase C110A mutant