4gzi - Revision history

OCA at 16:00, 14 March 2024

2024-03-14T16:00:19Z

← Older revision		Revision as of 16:00, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gzi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gzi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.68Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzi OCA], [https://pdbe.org/4gzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzi RCSB], [https://www.ebi.ac.uk/pdbsum/4gzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzi OCA], [https://pdbe.org/4gzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzi RCSB], [https://www.ebi.ac.uk/pdbsum/4gzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q70C53_SOLTU Q70C53_SOLTU]		[https://www.uniprot.org/uniprot/Q70C53_SOLTU Q70C53_SOLTU]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Plant endo-1,3-beta-glucanases are involved in important physiological processes such as defence mechanisms, cell division and flowering. They hydrolyze (1-->3)-beta-glucans, with very limited activity towards mixed (1-->3,1-->4)-beta-glucans and branched (1-->3,1-->6)-beta-glucans. Here, crystal structures of the potato (Solanum tuberosum) endo-1,3-beta-glucanase GLUB20-2 with the nucleophilic Glu259 residue substituted by alanine (E259A) are reported. Despite this active-site mutation, the protein retained residual endoglucanase activity and when incubated in the crystallization buffer with a linear hexameric substrate derived from (1-->3)-beta-glucan (laminarahexose) cleaved it in two different ways, generating trisaccharides and tetrasaccharides, as confirmed by mass spectrometry. The trisaccharide (laminaratriose) shows higher binding affinity and was found to fully occupy the -1, -2 and -3 sites of the active-site cleft, even at a low molar excess of the substrate. At elevated substrate concentration the tetrasaccharide molecule (laminaratetrose) also occupies the active site, spanning the opposite sites +1, +2, +3 and +4 of the cleft. These are the first crystal structures of a plant glycoside hydrolase family 17 (GH17) member to reveal the protein-saccharide interactions and were determined at resolutions of 1.68 and 1.55 A, respectively. The geometry of the active-site cleft clearly precludes any (1-->4)-beta-glucan topology at the subsites from -3 to +4 and could possibly accommodate beta-1,6-branching only at subsites +1 and +2. The glucose units at subsites -1 and -2 interact with highly conserved protein residues. In contrast, subsites -3, +3 and +4 are variable, suggesting that the mode of glucose binding at these sites may vary between different plant endo-1,3-beta-glucanases. Low substrate affinity is observed at subsites +1 and +2, as manifested by disorder of the glycosyl units there.

	Structures of an active-site mutant of a plant 1,3-beta-glucanase in complex with oligosaccharide products of hydrolysis.,Wojtkowiak A, Witek K, Hennig J, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):52-62. doi:, 10.1107/S0907444912042175. Epub 2012 Dec 20. PMID:23275163<ref>PMID:23275163</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4gzi" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]		*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:49, 3 November 2022

2022-11-03T07:49:47Z

← Older revision		Revision as of 07:49, 3 November 2022
Line 1:		Line 1:

	==Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose==		==Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose==
	<StructureSection load='4gzi' size='340' side='right' caption='[[4gzi]], [[Resolution\|resolution]] 1.68Å' scene=''>		<StructureSection load='4gzi' size='340' side='right'caption='[[4gzi]], [[Resolution\|resolution]] 1.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gzi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Potato Potato~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GZI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gzi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GZI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ur7\|3ur7]], [[3ur8\|3ur8]], [[1ghs\|1ghs]], [[2cyg\|2cyg]], [[3em5\|3em5]], [[3f55\|3f55]], [[4gzj\|4gzj]]</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzi OCA], [https://pdbe.org/4gzi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gzi RCSB], [https://www.ebi.ac.uk/pdbsum/4gzi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzi ProSAT]</span></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato])</td></tr>~~
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzi OCA], [~~http~~://pdbe.org/4gzi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gzi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gzi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzi ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q70C53_SOLTU Q70C53_SOLTU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4gzi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gzi" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
			*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Glucan endo-1,3-beta-D-glucosidase~~]]		[[Category: Large Structures]]
	[[Category: ~~Potato~~]]		[[Category: Solanum tuberosum]]
	[[Category: Hennig, J]]		[[Category: Hennig J]]
	[[Category: Jaskolski, M]]		[[Category: Jaskolski M]]
	[[Category: Witek, K]]		[[Category: Witek K]]
	[[Category: Wojtkowiak, A]]		[[Category: Wojtkowiak A]]
	~~[[Category: Carbohydrate/sugar binding]]~~
	~~[[Category: Catalytic nucleophile mutation]]~~
	~~[[Category: Gh17 family]]~~
	~~[[Category: Glucoside hydrolase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Laminaratriose]]~~
	~~[[Category: Tim barrel~~]]

OCA at 11:53, 6 November 2017

2017-11-06T11:53:23Z

← Older revision		Revision as of 11:53, 6 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4gzi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gzi" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Beta-glucosidase\|Beta-glucosidase]]
	*[[Glucanase\|Glucanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:43, 11 August 2016

2016-08-11T17:43:46Z

← Older revision		Revision as of 17:43, 11 August 2016
Line 1:		Line 1:
	~~[[Image:4gzi.png\|left\|200px]]~~

	~~{{STRUCTURE_4gzi~~\| ~~PDB~~=4gzi \| ~~SCENE~~= }}		==Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose==
			<StructureSection load='4gzi' size='340' side='right' caption='[[4gzi]], [[Resolution\|resolution]] 1.68Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4gzi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Potato Potato]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZI FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ur7\|3ur7]], [[3ur8\|3ur8]], [[1ghs\|1ghs]], [[2cyg\|2cyg]], [[3em5\|3em5]], [[3f55\|3f55]], [[4gzj\|4gzj]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Potato])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzi OCA], [http://pdbe.org/4gzi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gzi RCSB], [http://www.ebi.ac.uk/pdbsum/4gzi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gzi ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Plant endo-1,3-beta-glucanases are involved in important physiological processes such as defence mechanisms, cell division and flowering. They hydrolyze (1-->3)-beta-glucans, with very limited activity towards mixed (1-->3,1-->4)-beta-glucans and branched (1-->3,1-->6)-beta-glucans. Here, crystal structures of the potato (Solanum tuberosum) endo-1,3-beta-glucanase GLUB20-2 with the nucleophilic Glu259 residue substituted by alanine (E259A) are reported. Despite this active-site mutation, the protein retained residual endoglucanase activity and when incubated in the crystallization buffer with a linear hexameric substrate derived from (1-->3)-beta-glucan (laminarahexose) cleaved it in two different ways, generating trisaccharides and tetrasaccharides, as confirmed by mass spectrometry. The trisaccharide (laminaratriose) shows higher binding affinity and was found to fully occupy the -1, -2 and -3 sites of the active-site cleft, even at a low molar excess of the substrate. At elevated substrate concentration the tetrasaccharide molecule (laminaratetrose) also occupies the active site, spanning the opposite sites +1, +2, +3 and +4 of the cleft. These are the first crystal structures of a plant glycoside hydrolase family 17 (GH17) member to reveal the protein-saccharide interactions and were determined at resolutions of 1.68 and 1.55 A, respectively. The geometry of the active-site cleft clearly precludes any (1-->4)-beta-glucan topology at the subsites from -3 to +4 and could possibly accommodate beta-1,6-branching only at subsites +1 and +2. The glucose units at subsites -1 and -2 interact with highly conserved protein residues. In contrast, subsites -3, +3 and +4 are variable, suggesting that the mode of glucose binding at these sites may vary between different plant endo-1,3-beta-glucanases. Low substrate affinity is observed at subsites +1 and +2, as manifested by disorder of the glycosyl units there.

	~~===Active~~-site mutant of ~~potato endo-~~1,3-beta-glucanase in complex with ~~laminaratriose===~~		Structures of an active-site mutant of a plant 1,3-beta-glucanase in complex with oligosaccharide products of hydrolysis.,Wojtkowiak A, Witek K, Hennig J, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):52-62. doi:, 10.1107/S0907444912042175. Epub 2012 Dec 20. PMID:23275163<ref>PMID:23275163</ref>

	~~{{ABSTRACT_PUBMED_23275163}}~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~==About this Structure==~~		<div class="pdbe-citations 4gzi" style="background-color:#fffaf0;"></div>
	~~[[4gzi]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Solanum_tuberosum Solanum tuberosum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca~~-~~bin~~/~~ocashort?id=4GZI OCA].~~

	==See Also==		==See Also==
	*[[Beta-glucosidase\|Beta-glucosidase]]		*[[Beta-glucosidase\|Beta-glucosidase]]
			*[[Glucanase\|Glucanase]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Glucan endo-1,3-beta-D-glucosidase]]		[[Category: Glucan endo-1,3-beta-D-glucosidase]]
	[[Category: ~~Solanum tuberosum~~]]		[[Category: Potato]]
	[[Category: Hennig, J.]]		[[Category: Hennig, J]]
	[[Category: Jaskolski, M.]]		[[Category: Jaskolski, M]]
	[[Category: Witek, K.]]		[[Category: Witek, K]]
	[[Category: Wojtkowiak, A.]]		[[Category: Wojtkowiak, A]]
	[[Category: Carbohydrate/sugar binding]]		[[Category: Carbohydrate/sugar binding]]
	[[Category: Catalytic nucleophile mutation]]		[[Category: Catalytic nucleophile mutation]]

OCA at 11:27, 30 January 2013

2013-01-30T11:27:52Z

← Older revision		Revision as of 11:27, 30 January 2013
Line 9:		Line 9:
	==About this Structure==		==About this Structure==
	[[4gzi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA].		[[4gzi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA].

			==See Also==
			*[[Beta-glucosidase\|Beta-glucosidase]]
	[[Category: Glucan endo-1,3-beta-D-glucosidase]]		[[Category: Glucan endo-1,3-beta-D-glucosidase]]
	[[Category: Solanum tuberosum]]		[[Category: Solanum tuberosum]]

OCA at 11:34, 23 January 2013

2013-01-23T11:34:37Z

← Older revision		Revision as of 11:34, 23 January 2013
Line 1:		Line 1:
	[[Image:4gzi.~~jpg~~\|left\|200px]]		[[Image:4gzi.png\|left\|200px]]

	{{STRUCTURE_4gzi\| PDB=4gzi \| SCENE= }}		{{STRUCTURE_4gzi\| PDB=4gzi \| SCENE= }}
Line 5:		Line 5:
	===Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose===		===Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose===

			{{ABSTRACT_PUBMED_23275163}}

	==About this Structure==		==About this Structure==

OCA at 09:37, 2 January 2013

2013-01-02T09:37:15Z

← Older revision		Revision as of 09:37, 2 January 2013
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		[[Image:4gzi.jpg\|left\|200px]]

	~~The entry~~ 4gzi ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_4gzi\| PDB=4gzi \| SCENE= }}

	~~Authors: Wojtkowiak~~, ~~A., Witek, K., Hennig, J., Jaskolski, M.~~		===Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose===

	~~Description~~: ~~Active~~-~~site mutant of potato~~ endo-1,3-beta-~~glucanase in complex with laminaratriose~~
			==About this Structure==
			[[4gzi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZI OCA].
			[[Category: Glucan endo-1,3-beta-D-glucosidase]]
			[[Category: Solanum tuberosum]]
			[[Category: Hennig, J.]]
			[[Category: Jaskolski, M.]]
			[[Category: Witek, K.]]
			[[Category: Wojtkowiak, A.]]
			[[Category: Carbohydrate/sugar binding]]
			[[Category: Catalytic nucleophile mutation]]
			[[Category: Gh17 family]]
			[[Category: Glucoside hydrolase]]
			[[Category: Hydrolase]]
			[[Category: Laminaratriose]]
			[[Category: Tim barrel]]

OCA at 09:02, 21 November 2012

2012-11-21T09:02:19Z

← Older revision		Revision as of 09:02, 21 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4gzi is ON HOLD		The entry 4gzi is ON HOLD until Paper Publication

	Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M.		Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M.

	Description: Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose		Description: Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose

OCA: Protected "4gzi" [edit=sysop:move=sysop]

2012-09-12T04:57:41Z

Protected "4gzi" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:57, 12 September 2012
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4gzi is ON HOLD Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M. Description: Active-site mutant of potato endo-1,3-beta-glucanase in ...

2012-09-12T04:57:40Z

New page: '''Unreleased structure''' The entry 4gzi is ON HOLD Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M. Description: Active-site mutant of potato endo-1,3-beta-glucanase in ...

New page

'''Unreleased structure'''

The entry 4gzi is ON HOLD

Authors: Wojtkowiak, A., Witek, K., Hennig, J., Jaskolski, M.

Description: Active-site mutant of potato endo-1,3-beta-glucanase in complex with laminaratriose