4iph - Revision history

OCA at 15:28, 20 September 2023

2023-09-20T15:28:31Z

← Older revision		Revision as of 15:28, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1FJ:N-(2,3-DIMETHYLPHENYL)-5-OXO-1-THIOXO-1,5-DIHYDRO-2LAMBDA~4~-[1,3]THIAZOLO[3,4-A]THIENO[2,3-E]PYRIMIDINE-3-CARBOXAMIDE'>1FJ</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1FJ:N-(2,3-DIMETHYLPHENYL)-5-OXO-1-THIOXO-1,5-DIHYDRO-2LAMBDA~4~-[1,3]THIAZOLO[3,4-A]THIENO[2,3-E]PYRIMIDINE-3-CARBOXAMIDE'>1FJ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iph OCA], [https://pdbe.org/4iph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iph RCSB], [https://www.ebi.ac.uk/pdbsum/4iph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iph ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iph OCA], [https://pdbe.org/4iph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iph RCSB], [https://www.ebi.ac.uk/pdbsum/4iph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iph ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:02, 16 November 2022

2022-11-16T21:02:18Z

← Older revision		Revision as of 21:02, 16 November 2022
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	<StructureSection load='4iph' size='340' side='right'caption='[[4iph]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='4iph' size='340' side='right'caption='[[4iph]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IPH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IPH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1FJ:N-(2,3-DIMETHYLPHENYL)-5-OXO-1-THIOXO-1,5-DIHYDRO-2LAMBDA~4~-[1,3]THIAZOLO[3,4-A]THIENO[2,3-E]PYRIMIDINE-3-CARBOXAMIDE'>1FJ</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1FJ:N-(2,3-DIMETHYLPHENYL)-5-OXO-1-THIOXO-1,5-DIHYDRO-2LAMBDA~4~-[1,3]THIAZOLO[3,4-A]THIENO[2,3-E]PYRIMIDINE-3-CARBOXAMIDE'>1FJ</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ipc\|4ipc]], [[4ipd\|4ipd]], [[4ipg\|4ipg]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iph OCA], [https://pdbe.org/4iph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iph RCSB], [https://www.ebi.ac.uk/pdbsum/4iph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iph ProSAT]</span></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RPA1, REPA1, RPA70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4iph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iph OCA], [~~http~~://pdbe.org/4iph PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4iph RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4iph PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4iph ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/RFA1_HUMAN RFA1_HUMAN]] Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref> Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref>		[https://www.uniprot.org/uniprot/RFA1_HUMAN RFA1_HUMAN] Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref> Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chazin~~, W J~~]]		[[Category: Chazin WJ]]
	[[Category: Feldkamp~~, M D~~]]		[[Category: Feldkamp MD]]
	[[Category: Fesik~~, S W~~]]		[[Category: Fesik SW]]
	[[Category: Frank~~, A O~~]]		[[Category: Frank AO]]
	[[Category: Vangamudi, B]]		[[Category: Vangamudi B]]
	~~[[Category: Ob-fold]]~~
	~~[[Category: Protein binding]]~~
	~~[[Category: Protein binding-inhibitor complex~~]]

OCA at 07:00, 19 February 2020

2020-02-19T07:00:20Z

← Older revision		Revision as of 07:00, 19 February 2020
Line 1:		Line 1:

	==Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor==		==Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor==
	<StructureSection load='4iph' size='340' side='right' caption='[[4iph]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='4iph' size='340' side='right'caption='[[4iph]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPH FirstGlance]. <br>
Line 22:		Line 22:

	==See Also==		==See Also==
	*[[Single-stranded DNA-binding protein\|Single-stranded DNA-binding protein]]		*[[Single-stranded DNA-binding protein 3D structures\|Single-stranded DNA-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Chazin, W J]]		[[Category: Chazin, W J]]
	[[Category: Feldkamp, M D]]		[[Category: Feldkamp, M D]]

OCA at 15:10, 4 August 2016

2016-08-04T15:10:35Z

← Older revision		Revision as of 15:10, 4 August 2016
Line 1:		Line 1:

	==Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor==		==Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor==
	<StructureSection load='4iph' size='340' side='right' caption='[[4iph]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='4iph' size='340' side='right' caption='[[4iph]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_mikatae Saccharomyces mikatae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPH FirstGlance]. <br>		<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1FJ:N-(2,3-DIMETHYLPHENYL)-5-OXO-1-THIOXO-1,5-DIHYDRO-2LAMBDA~4~-[1,3]THIAZOLO[3,4-A]THIENO[2,3-E]PYRIMIDINE-3-CARBOXAMIDE'>1FJ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1FJ:N-(2,3-DIMETHYLPHENYL)-5-OXO-1-THIOXO-1,5-DIHYDRO-2LAMBDA~4~-[1,3]THIAZOLO[3,4-A]THIENO[2,3-E]PYRIMIDINE-3-CARBOXAMIDE'>1FJ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ipc\|4ipc]], [[4ipd\|4ipd]], [[4ipg\|4ipg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ipc\|4ipc]], [[4ipd\|4ipd]], [[4ipg\|4ipg]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RPA1, REPA1, RPA70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~114525 Saccharomyces mikatae~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RPA1, REPA1, RPA70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iph OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iph RCSB], [http://www.ebi.ac.uk/pdbsum/4iph PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iph OCA], [http://pdbe.org/4iph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iph RCSB], [http://www.ebi.ac.uk/pdbsum/4iph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iph ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4iph" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Saccharomyces mikatae~~]]		[[Category: Human]]
	[[Category: Chazin, W J]]		[[Category: Chazin, W J]]
	[[Category: Feldkamp, M D]]		[[Category: Feldkamp, M D]]

OCA at 10:25, 25 December 2014

2014-12-25T10:25:32Z

← Older revision		Revision as of 10:25, 25 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4iph\| PDB=4iph \| SCENE= }}~~		==Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor==
	===Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor===		<StructureSection load='4iph' size='340' side='right' caption='[[4iph]], [[Resolution\|resolution]] 1.94Å' scene=''>
	~~{{ABSTRACT_PUBMED_23962067}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_mikatae Saccharomyces mikatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IPH FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1FJ:N-(2,3-DIMETHYLPHENYL)-5-OXO-1-THIOXO-1,5-DIHYDRO-2LAMBDA~4~-[1,3]THIAZOLO[3,4-A]THIENO[2,3-E]PYRIMIDINE-3-CARBOXAMIDE'>1FJ</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ipc\|4ipc]], [[4ipd\|4ipd]], [[4ipg\|4ipg]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RPA1, REPA1, RPA70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=114525 Saccharomyces mikatae])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iph OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iph RCSB], [http://www.ebi.ac.uk/pdbsum/4iph PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RFA1_HUMAN RFA1_HUMAN]] Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref> Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Replication protein A (RPA) is the primary single-stranded DNA (ssDNA) binding protein in eukaryotes. The N-terminal domain of the RPA70 subunit (RPA70N) interacts via a basic cleft with a wide range of DNA processing proteins, including several that regulate DNA damage response and repair. Small molecule inhibitors that disrupt these protein-protein interactions are therefore of interest as chemical probes of these critical DNA processing pathways and as inhibitors to counter the upregulation of DNA damage response and repair associated with treatment of cancer patients with radiation or DNA-damaging agents. Determination of three-dimensional structures of protein-ligand complexes is an important step for elaboration of small molecule inhibitors. However, although crystal structures of free RPA70N and an RPA70N-peptide fusion construct have been reported, RPA70N-inhibitor complexes have been recalcitrant to crystallization. Analysis of the P61 lattice of RPA70N crystals led us to hypothesize that the ligand-binding surface was occluded. Surface reengineering to alter key crystal lattice contacts led to the design of RPA70N E7R, E100R, and E7R/E100R mutants. These mutants crystallized in a P212121 lattice that clearly had significant solvent channels open to the critical basic cleft. Analysis of X-ray crystal structures, target peptide binding affinities, and 15N-1H heteronuclear single-quantum coherence nuclear magnetic resonance spectra showed that the mutations do not result in perturbations of the RPA70N ligand-binding surface. The success of the design was demonstrated by determining the structure of RPA70N E7R soaked with a ligand discovered in a previously reported molecular fragment screen. A fluorescence anisotropy competition binding assay revealed this compound can inhibit the interaction of RPA70N with the peptide binding motif from the DNA damage response protein ATRIP. The implications of the results are discussed in the context of ongoing efforts to design RPA70N inhibitors.

	~~==Function==~~		Surface Reengineering of RPA70N Enables Cocrystallization with an Inhibitor of the Replication Protein A Interaction Motif of ATR Interacting Protein.,Feldkamp MD, Frank AO, Kennedy JP, Patrone JD, Vangamudi B, Waterson AG, Fesik SW, Chazin WJ Biochemistry. 2013 Sep 6. PMID:23962067<ref>PMID:23962067</ref>
	~~[[http://www.uniprot.org/uniprot/RFA1_HUMAN RFA1_HUMAN]] Plays~~ an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref> Functions as component of the ~~alternative replication protein~~ A ~~complex (aRPA)~~. ~~aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro~~, ~~aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC)~~, ~~the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange~~.~~<ref>~~PMID:~~19116208</ref>~~ <ref>PMID:~~19996105~~</ref>

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[4iph]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_mikatae Saccharomyces mikatae]~~. ~~Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA].~~		</div>

	==~~Reference~~==		==See Also==
	~~<ref group~~=~~"xtra">PMID:023962067</ref>~~<references ~~group="xtra"~~/><~~references~~/>		*[[Single-stranded DNA-binding protein\|Single-stranded DNA-binding protein]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Saccharomyces mikatae]]		[[Category: Saccharomyces mikatae]]
	[[Category: Chazin, W J.]]		[[Category: Chazin, W J]]
	[[Category: Feldkamp, M D.]]		[[Category: Feldkamp, M D]]
	[[Category: Fesik, S W.]]		[[Category: Fesik, S W]]
	[[Category: Frank, A O.]]		[[Category: Frank, A O]]
	[[Category: Vangamudi, B.]]		[[Category: Vangamudi, B]]
	[[Category: Ob-fold]]		[[Category: Ob-fold]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Protein binding-inhibitor complex]]		[[Category: Protein binding-inhibitor complex]]

OCA at 06:00, 2 October 2013

2013-10-02T06:00:49Z

← Older revision		Revision as of 06:00, 2 October 2013
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
	[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA].		[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_mikatae Saccharomyces mikatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023962067</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023962067</ref><references group="xtra"/><references/>
	[[Category: ~~Homo sapiens~~]]		[[Category: Saccharomyces mikatae]]
	[[Category: Chazin, W J.]]		[[Category: Chazin, W J.]]
	[[Category: Feldkamp, M D.]]		[[Category: Feldkamp, M D.]]

OCA at 05:20, 12 September 2013

2013-09-12T05:20:14Z

← Older revision		Revision as of 05:20, 12 September 2013
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	~~'''Unreleased structure'''~~		{{STRUCTURE_4iph\| PDB=4iph \| SCENE= }}
			===Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor===
			{{ABSTRACT_PUBMED_23962067}}

	~~The entry 4iph is ON HOLD~~ ~~until Paper Publication~~		==Function==
			[[http://www.uniprot.org/uniprot/RFA1_HUMAN RFA1_HUMAN]] Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref> Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.<ref>PMID:19116208</ref> <ref>PMID:19996105</ref>

	~~Authors~~: ~~Feldkamp, M~~.D.~~, Frank, A~~.~~O., Vangamudi, B., Fesik, S~~.W.~~, Chazin, W~~.J.		==About this Structure==
			[[4iph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IPH OCA].

	~~Description~~: ~~Structure of N~~-~~terminal domain of RPA70 in~~ complex ~~with VU079104 inhibitor~~		==Reference==
			<ref group="xtra">PMID:023962067</ref><references group="xtra"/><references/>
			[[Category: Homo sapiens]]
			[[Category: Chazin, W J.]]
			[[Category: Feldkamp, M D.]]
			[[Category: Fesik, S W.]]
			[[Category: Frank, A O.]]
			[[Category: Vangamudi, B.]]
			[[Category: Ob-fold]]
			[[Category: Protein binding]]
			[[Category: Protein binding-inhibitor complex]]

OCA at 12:02, 30 January 2013

2013-01-30T12:02:50Z

← Older revision		Revision as of 12:02, 30 January 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4iph is ON HOLD		The entry 4iph is ON HOLD until Paper Publication

	Authors: Feldkamp, M.D., Frank, A.O., Vangamudi, B., Fesik, S.W., Chazin, W.J.		Authors: Feldkamp, M.D., Frank, A.O., Vangamudi, B., Fesik, S.W., Chazin, W.J.

	Description: ~~Surface engineering~~ of ~~the~~ N-terminal domain of ~~RPA70N to obtain X-ray structures of co-complexes~~		Description: Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor

OCA: Protected "4iph" [edit=sysop:move=sysop]

2013-01-16T08:28:58Z

Protected "4iph" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:28, 16 January 2013
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4iph is ON HOLD Authors: Feldkamp, M.D., Frank, A.O., Vangamudi, B., Fesik, S.W., Chazin, W.J. Description: Surface engineering of the N-terminal d...

2013-01-16T08:28:57Z

New page: '''Unreleased structure''' The entry 4iph is ON HOLD Authors: Feldkamp, M.D., Frank, A.O., Vangamudi, B., Fesik, S.W., Chazin, W.J. Description: Surface engineering of the N-terminal d...

New page

'''Unreleased structure'''

The entry 4iph is ON HOLD

Authors: Feldkamp, M.D., Frank, A.O., Vangamudi, B., Fesik, S.W., Chazin, W.J.

Description: Surface engineering of the N-terminal domain of RPA70N to obtain X-ray structures of co-complexes