4lev - Revision history

OCA at 06:41, 17 October 2024

2024-10-17T06:41:10Z

← Older revision		Revision as of 06:41, 17 October 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4lev]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4lev]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LEV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.952Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lev OCA], [https://pdbe.org/4lev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lev RCSB], [https://www.ebi.ac.uk/pdbsum/4lev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lev ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lev OCA], [https://pdbe.org/4lev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lev RCSB], [https://www.ebi.ac.uk/pdbsum/4lev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lev ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:56, 14 December 2022

2022-12-14T10:56:46Z

← Older revision		Revision as of 10:56, 14 December 2022
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	==Structure of human cGAS==		==Structure of human cGAS==
	<StructureSection load='4lev' size='340' side='right' caption='[[4lev]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4lev' size='340' side='right'caption='[[4lev]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4lev]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LEV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4LEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4lev]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LEV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr~~ id~~='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class~~="~~sblockDat~~"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene><~~/td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lev OCA], [https://pdbe.org/4lev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lev RCSB], [https://www.ebi.ac.uk/pdbsum/4lev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lev ProSAT]</span></td></tr>
	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat">[[4lew\|4lew]], [[4ley\|4ley]], [[4lez\|4lez]]</td></tr>~~
	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">MB21D1, C6orf150 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lev OCA], [~~http~~://pdbe.org/4lev PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4lev RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4lev PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4lev ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/CGAS_HUMAN CGAS_HUMAN]] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.<ref>PMID:21478870</ref> <ref>PMID:23258413</ref>		[https://www.uniprot.org/uniprot/CGAS_HUMAN CGAS_HUMAN] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.<ref>PMID:21478870</ref> <ref>PMID:23258413</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Cyclic GMP-AMP synthase\|Cyclic GMP-AMP synthase]]		*[[Cyclic GMP-AMP synthase 3D synthase\|Cyclic GMP-AMP synthase 3D synthase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: ~~Li, P~~]]		[[Category: Large Structures]]
	[[Category: ~~Dna sensor]]~~		[[Category: Li P]]
	~~[[Category: Ntase]]~~
	~~[[Category: Transferase~~]]

OCA at 15:12, 5 August 2016

2016-08-05T15:12:34Z

← Older revision		Revision as of 15:12, 5 August 2016
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	==Structure of human cGAS==		==Structure of human cGAS==
	<StructureSection load='4lev' size='340' side='right' caption='[[4lev]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4lev' size='340' side='right' caption='[[4lev]], [[Resolution\|resolution]] 1.95Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lew\|4lew]], [[4ley\|4ley]], [[4lez\|4lez]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lew\|4lew]], [[4ley\|4ley]], [[4lez\|4lez]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MB21D1, C6orf150 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MB21D1, C6orf150 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lev RCSB], [http://www.ebi.ac.uk/pdbsum/4lev PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lev OCA], [http://pdbe.org/4lev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lev RCSB], [http://www.ebi.ac.uk/pdbsum/4lev PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lev ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4lev" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 19:50, 25 December 2014

2014-12-25T19:50:58Z

← Older revision		Revision as of 19:50, 25 December 2014
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	~~{{STRUCTURE_4lev~~\| ~~PDB~~=4lev \| ~~SCENE~~= }}		==Structure of human cGAS==
	===~~Structure~~ of ~~human cGAS~~===		<StructureSection load='4lev' size='340' side='right' caption='[[4lev]], [[Resolution\|resolution]] 1.95Å' scene=''>
	~~{{ABSTRACT_PUBMED_24332030}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4lev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LEV FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lew\|4lew]], [[4ley\|4ley]], [[4lez\|4lez]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MB21D1, C6orf150 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lev RCSB], [http://www.ebi.ac.uk/pdbsum/4lev PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CGAS_HUMAN CGAS_HUMAN]] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.<ref>PMID:21478870</ref> <ref>PMID:23258413</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor mediating innate antimicrobial immunity. It catalyzes the synthesis of a noncanonical cyclic dinucleotide, 2',5' cGAMP, that binds to STING and mediates the activation of TBK1 and IRF-3. Activated IRF-3 translocates to the nucleus and initiates the transcription of the IFN-beta gene. The structure of mouse cGAS bound to an 18 bp dsDNA revealed that cGAS interacts with dsDNA through two binding sites, forming a 2:2 complex. Enzyme assays and IFN-beta reporter assays of cGAS mutants demonstrated that interactions at both DNA binding sites are essential for cGAS activation. Mutagenesis and DNA binding studies showed that the two sites bind dsDNA cooperatively and that site B plays a critical role in DNA binding. The structure of mouse cGAS bound to dsDNA and 2',5' cGAMP provided insight into the catalytic mechanism of cGAS. These results demonstrated that cGAS is activated by dsDNA-induced oligomerization.

	~~==Function==~~		Cyclic GMP-AMP Synthase Is Activated by Double-Stranded DNA-Induced Oligomerization.,Li X, Shu C, Yi G, Chaton CT, Shelton CL, Diao J, Zuo X, Kao CC, Herr AB, Li P Immunity. 2013 Dec 12;39(6):1019-31. doi: 10.1016/j.immuni.2013.10.019. PMID:24332030<ref>PMID:24332030</ref>
	~~[[http://www.uniprot.org/uniprot/CGAS_HUMAN CGAS_HUMAN]] Nucleotidyltransferase that catalyzes formation of cyclic~~ GMP-AMP ~~(cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity~~ by ~~acting as a key cytosolic~~ DNA ~~sensor~~, ~~the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly~~, ~~leading to activation and synthesis of cGAMP~~, ~~a second messenger that binds to and activates TMEM173/STING~~, ~~thereby triggering type~~-~~I interferon production~~.~~<ref>~~PMID:~~21478870</ref>~~ <ref>PMID:~~23258413~~</ref>

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[4lev]] is~~ a ~~2 chain structure. Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=4LEV OCA].~~		</div>

	==~~Reference~~==		==See Also==
	~~<ref group~~=~~"xtra">PMID:024332030</ref>~~<references ~~group="xtra"~~/><~~references~~/>		*[[Cyclic GMP-AMP synthase\|Cyclic GMP-AMP synthase]]
	[[Category: Li, P.]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Human]]
			[[Category: Li, P]]
	[[Category: Dna sensor]]		[[Category: Dna sensor]]
	[[Category: Ntase]]		[[Category: Ntase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 08:27, 8 January 2014

2014-01-08T08:27:00Z

← Older revision		Revision as of 08:27, 8 January 2014
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	{{STRUCTURE_4lev\| PDB=4lev \| SCENE= }}		{{STRUCTURE_4lev\| PDB=4lev \| SCENE= }}
	===Structure of human cGAS===		===Structure of human cGAS===
			{{ABSTRACT_PUBMED_24332030}}

	==Function==		==Function==
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	==Reference==		==Reference==
	<references group="xtra"/><references/>		<ref group="xtra">PMID:024332030</ref><references group="xtra"/><references/>
	[[Category: Li, P.]]		[[Category: Li, P.]]
	[[Category: Dna sensor]]		[[Category: Dna sensor]]
	[[Category: Ntase]]		[[Category: Ntase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:26, 25 December 2013

2013-12-25T10:26:56Z

← Older revision		Revision as of 10:26, 25 December 2013
Line 1:		Line 1:
	~~'''Unreleased structure'''~~		{{STRUCTURE_4lev\| PDB=4lev \| SCENE= }}
			===Structure of human cGAS===

	~~The entry 4lev is ON HOLD~~ ~~until Paper Publication~~		==Function==
			[[http://www.uniprot.org/uniprot/CGAS_HUMAN CGAS_HUMAN]] Nucleotidyltransferase that catalyzes formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and exhibits antiviral activity. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of DNA in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.<ref>PMID:21478870</ref> <ref>PMID:23258413</ref>

	~~Authors~~: ~~Li, P~~.		==About this Structure==
			[[4lev]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LEV OCA].

	~~Description~~: ~~Structure of human cGAS~~		==Reference==
			<references group="xtra"/><references/>
			[[Category: Li, P.]]
			[[Category: Dna sensor]]
			[[Category: Ntase]]
			[[Category: Transferase]]

OCA at 12:46, 20 November 2013

2013-11-20T12:46:09Z

← Older revision		Revision as of 12:46, 20 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4lev is ON HOLD		The entry 4lev is ON HOLD until Paper Publication

	Authors: Li, P.		Authors: Li, P.

	Description: Structure of human cGAS		Description: Structure of human cGAS

OCA: Protected "4lev" [edit=sysop:move=sysop]

2013-07-03T13:27:38Z

Protected "4lev" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:27, 3 July 2013
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4lev is ON HOLD Authors: Li, P. Description: Structure of human cGAS

2013-07-03T13:27:36Z

New page: '''Unreleased structure''' The entry 4lev is ON HOLD Authors: Li, P. Description: Structure of human cGAS

New page

'''Unreleased structure'''

The entry 4lev is ON HOLD

Authors: Li, P.

Description: Structure of human cGAS