4moa - Revision history

OCA at 14:44, 8 November 2023

2023-11-08T14:44:07Z

← Older revision		Revision as of 14:44, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis Bacillus thuringiensis serovar israelensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis Bacillus thuringiensis serovar israelensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [https://pdbe.org/4moa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [https://www.ebi.ac.uk/pdbsum/4moa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [https://pdbe.org/4moa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [https://www.ebi.ac.uk/pdbsum/4moa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:57, 28 December 2022

2022-12-28T09:57:19Z

← Older revision		Revision as of 09:57, 28 December 2022
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	<StructureSection load='4moa' size='340' side='right'caption='[[4moa]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4moa' size='340' side='right'caption='[[4moa]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacti Bacti~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MOA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis Bacillus thuringiensis serovar israelensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOA FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cry4Ba, bt8, cryD2, cryIVB(a), isrH3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1430 BACTI])</td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [https://pdbe.org/4moa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [https://www.ebi.ac.uk/pdbsum/4moa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [~~http~~://pdbe.org/4moa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4moa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.		[https://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacti~~]]		[[Category: Bacillus thuringiensis serovar israelensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Angsuthanasombat, C]]		[[Category: Angsuthanasombat C]]
	[[Category: Chen, C J]]		[[Category: Chen C-J]]
	[[Category: Thamwiriyasati, N]]		[[Category: Thamwiriyasati N]]
	~~[[Category: Pesticidal protein]]~~
	~~[[Category: Toxin~~]]

OCA at 08:56, 10 April 2019

2019-04-10T08:56:34Z

← Older revision		Revision as of 08:56, 10 April 2019
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	==Crystal structure of CRY4BA-R203Q TOXIN==		==Crystal structure of CRY4BA-R203Q TOXIN==
	<StructureSection load='4moa' size='340' side='right' caption='[[4moa]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4moa' size='340' side='right'caption='[[4moa]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacti Bacti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [http://pdbe.org/4moa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [http://www.ebi.ac.uk/pdbsum/4moa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cry4Ba, bt8, cryD2, cryIVB(a), isrH3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1430 BACTI])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [http://pdbe.org/4moa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [http://www.ebi.ac.uk/pdbsum/4moa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	==See Also==		==See Also==
	*[[Delta-endotoxin\|Delta-endotoxin]]		*[[Delta-endotoxin\|Delta-endotoxin]]
			*[[Pesticidal crystal protein\|Pesticidal crystal protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacti]]
			[[Category: Large Structures]]
	[[Category: Angsuthanasombat, C]]		[[Category: Angsuthanasombat, C]]
	[[Category: Chen, C J]]		[[Category: Chen, C J]]

OCA at 11:31, 15 December 2016

2016-12-15T11:31:58Z

← Older revision		Revision as of 11:31, 15 December 2016
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.		[[http://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The insecticidal feature of the three-domain Cry delta-endotoxins from Bacillus thuringiensis is generally attributed to their capability to form oligomeric pores, causing lysis of target larval midgut cells. However, the molecular description of their oligomerization process has not been clearly defined. Here a stable prepore of the 65-kDa trypsin-activated Cry4Ba mosquito-specific toxin was established through membrane-mimetic environments by forming an approximately 200-kDa octyl-beta-D-glucoside micelle-induced trimer. The SDS-resistant trimer caused cytolysis to Sf9 insect cells expressing Aedes-mALP (a Cry4Ba receptor) and was more effective than a toxin monomer in membrane perturbation of calcein-loaded liposomes. A three-dimensional model of toxin trimer obtained by negative-stain EM in combination with single-particle reconstruction at approximately 5 nm resolution showed a propeller-shaped structure with 3-fold symmetry. Fitting the three-dimensional reconstructed EM map with a 100-ns molecular dynamics-simulated Cry4Ba structure interacting with an octyl-beta-D-glucoside micelle showed relative positioning of individual domains in the context of the trimeric complex with a major protrusion from the pore-forming domain. Moreover, high-speed atomic force microscopy imaging at nanometer resolution and a subsecond frame rate demonstrated conformational transitions from a propeller-like to a globularly shaped trimer upon lipid membrane interactions, implying prepore-to-pore conversion. Real-time trimeric arrangement of monomers associated with L-alpha-dimyristoylphosphatidylcholine/3-[(3-cholamidopropyl)dimethylammonio]-2-h ydroxy-1-propanesulfonic acid bicelle membranes was also envisaged by successive high-speed atomic force microscopy imaging, depicting interactions among three individual subunits toward trimer formation. Together, our data provide the first pivotal insights into the structural requirement of membrane-induced conformational changes of Cry4Ba toxin monomers for the molecular assembly of a prepore trimer capable of inserting into target membranes to generate a lytic pore.

			Potential Prepore Trimer Formation by the Bacillus thuringiensis Mosquito-specific Toxin: MOLECULAR INSIGHTS INTO A CRITICAL PREREQUISITE OF MEMBRANE-BOUND MONOMERS.,Sriwimol W, Aroonkesorn A, Sakdee S, Kanchanawarin C, Uchihashi T, Ando T, Angsuthanasombat C J Biol Chem. 2015 Aug 21;290(34):20793-803. doi: 10.1074/jbc.M114.627554. Epub, 2015 Jun 25. PMID:26112409<ref>PMID:26112409</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4moa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Delta-endotoxin\|Delta-endotoxin]]		*[[Delta-endotoxin\|Delta-endotoxin]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 22:18, 4 August 2016

2016-08-04T22:18:40Z

← Older revision		Revision as of 22:18, 4 August 2016
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	==Crystal structure of CRY4BA-R203Q TOXIN==		==Crystal structure of CRY4BA-R203Q TOXIN==
	<StructureSection load='4moa' size='340' side='right' caption='[[4moa]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4moa' size='340' side='right' caption='[[4moa]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [http://www.ebi.ac.uk/pdbsum/4moa PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [http://pdbe.org/4moa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [http://www.ebi.ac.uk/pdbsum/4moa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4moa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.		[[http://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.

			==See Also==
			*[[Delta-endotoxin\|Delta-endotoxin]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:10, 24 December 2014

2014-12-24T20:10:24Z

← Older revision		Revision as of 20:10, 24 December 2014
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [http://www.ebi.ac.uk/pdbsum/4moa PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [http://www.ebi.ac.uk/pdbsum/4moa PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CR4BA_BACTI CR4BA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:20, 10 December 2014

2014-12-10T16:20:25Z

← Older revision		Revision as of 16:20, 10 December 2014
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	'''~~Unreleased structure~~'''		==Crystal structure of CRY4BA-R203Q TOXIN==
			<StructureSection load='4moa' size='340' side='right' caption='[[4moa]], [[Resolution\|resolution]] 2.00Å' scene=''>
	~~The entry~~ 4moa is ~~ON HOLD~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4moa]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOA FirstGlance]. <br>
	~~Authors~~: ~~Thamwiriyasati~~, N., Angsuthanasombat, C., Chen, C.-J.		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4moa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4moa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4moa RCSB], [http://www.ebi.ac.uk/pdbsum/4moa PDBsum]</span></td></tr>
			</table>
	~~Description~~: ~~Crystal structure of CRY4BA-R203Q TOXIN~~		__TOC__
			</StructureSection>
			[[Category: Angsuthanasombat, C]]
			[[Category: Chen, C J]]
			[[Category: Thamwiriyasati, N]]
			[[Category: Pesticidal protein]]
			[[Category: Toxin]]

OCA at 09:08, 18 December 2013

2013-12-18T09:08:46Z

← Older revision		Revision as of 09:08, 18 December 2013
Line 3:		Line 3:
	The entry 4moa is ON HOLD		The entry 4moa is ON HOLD

	Authors: Thamwiriyasati, N., Angsuthanasombat, C., Chen, C. -J.		Authors: Thamwiriyasati, N., Angsuthanasombat, C., Chen, C.-J.

	Description: Crystal structure of CRY4BA-R203Q TOXIN		Description: Crystal structure of CRY4BA-R203Q TOXIN

OCA: Protected "4moa" [edit=sysop:move=sysop]

2013-09-20T03:44:28Z

Protected "4moa" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:44, 20 September 2013
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4moa is ON HOLD Authors: Thamwiriyasati, N., Angsuthanasombat, C., Chen, C. -J. Description: Crystal structure of CRY4BA-R203Q TOXIN

2013-09-20T03:44:27Z

New page: '''Unreleased structure''' The entry 4moa is ON HOLD Authors: Thamwiriyasati, N., Angsuthanasombat, C., Chen, C. -J. Description: Crystal structure of CRY4BA-R203Q TOXIN

New page

'''Unreleased structure'''

The entry 4moa is ON HOLD

Authors: Thamwiriyasati, N., Angsuthanasombat, C., Chen, C. -J.

Description: Crystal structure of CRY4BA-R203Q TOXIN