4mod - Revision history

OCA at 14:44, 8 November 2023

2023-11-08T14:44:08Z

← Older revision		Revision as of 14:44, 8 November 2023
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	==Structure of the MERS-CoV fusion core==		==Structure of the MERS-CoV fusion core==
	<StructureSection load='4mod' size='340' side='right'caption='[[4mod]]' scene=''>		<StructureSection load='4mod' size='340' side='right'caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOD FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_betacoronavirus_2c_EMC/2012 Human betacoronavirus 2c EMC/2012] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOD FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [https://pdbe.org/4mod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [https://www.ebi.ac.uk/pdbsum/4mod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.901Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [https://pdbe.org/4mod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [https://www.ebi.ac.uk/pdbsum/4mod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/SPIKE_MERS1 SPIKE_MERS1] Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Interacts with host DPP4 to mediate virla entry.[HAMAP-Rule:MF_04099]<ref>PMID:23486063</ref> Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Middle East respiratory syndrome coronavirus (MERS-CoV) recently emerged as a severe worldwide public health concern. The virus is highly pathogenic, manifesting in infected patients with an approximately 50% fatality rate. It is known that the surface spike (S) proteins of coronaviruses mediate receptor recognition and membrane fusion, thereby playing an indispensable role in initiating infection. In this process, the heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called the fusion core, which represents a key membrane fusion architecture. To date, however, the MERS-CoV fusion core remains uncharacterized thus far. Here, we performed a series of biochemical and biophysical analyses characterizing the HR1/HR2 complexes of this novel virus. The HR sequences were variably truncated and then connected with a flexible amino acid linker. In each case, the recombinant protein automatically assembled into a trimer in solution, displaying a typical alpha-helical structure. One of these trimers was successfully crystallized, and its structure was solved at a resolution of 1.9 A. A canonical 6-helix bundle, like those reported for other coronaviruses, was revealed, with three HR1 helices forming the central coiled-coil core and three HR2 chains surrounding the core in the HR1 side grooves. This demonstrates that MERS-CoV utilizes a mechanism similar to other class I enveloped viruses for membrane fusion. With this notion, we further identified an HR2-based peptide that could potently inhibit MERS-CoV fusion and entry by using a pseudotyped-virus system. These results lay the ground for future inhibitory peptidic drug design.

			Structure of the fusion core and inhibition of fusion by a heptad-repeat peptide derived from the S protein of MERS-CoV.,Gao J, Lu G, Qi J, Li Y, Wu Y, Deng Y, Geng H, Li H, Wang Q, Xiao H, Tan W, Yan J, Gao GF J Virol. 2013 Sep 25. PMID:24067982<ref>PMID:24067982</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4mod" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Sandbox 3001\|Sandbox 3001]]		*[[Sandbox 3001\|Sandbox 3001]]
	*[[Spike protein\|Spike protein]]		*[[Spike protein\|Spike protein]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Human betacoronavirus 2c EMC/2012]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Synthetic construct]]
	[[Category: Deng Y]]		[[Category: Deng Y]]
	[[Category: Gao GF]]		[[Category: Gao GF]]

OCA at 10:48, 31 August 2022

2022-08-31T10:48:15Z

← Older revision		Revision as of 10:48, 31 August 2022
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	==Structure of the MERS-CoV fusion core==		==Structure of the MERS-CoV fusion core==
	<StructureSection load='4mod' size='340' side='right'caption='[[4mod]]~~, [[Resolution\|resolution]] 1.90Å~~' scene=''>		<StructureSection load='4mod' size='340' side='right'caption='[[4mod]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[4mod]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOD FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOD FirstGlance]. <br>
	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wnc\|1wnc]], [[1wdf\|1wdf]]</div></td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [https://pdbe.org/4mod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [https://www.ebi.ac.uk/pdbsum/4mod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [https://pdbe.org/4mod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [https://www.ebi.ac.uk/pdbsum/4mod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The Middle East respiratory syndrome coronavirus (MERS-CoV) recently emerged as a severe worldwide public health concern. The virus is highly pathogenic, manifesting in infected patients with an approximately 50% fatality rate. It is known that the surface spike (S) proteins of coronaviruses mediate receptor recognition and membrane fusion, thereby playing an indispensable role in initiating infection. In this process, the heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called the fusion core, which represents a key membrane fusion architecture. To date, however, the MERS-CoV fusion core remains uncharacterized thus far. Here, we performed a series of biochemical and biophysical analyses characterizing the HR1/HR2 complexes of this novel virus. The HR sequences were variably truncated and then connected with a flexible amino acid linker. In each case, the recombinant protein automatically assembled into a trimer in solution, displaying a typical alpha-helical structure. One of these trimers was successfully crystallized, and its structure was solved at a resolution of 1.9 A. A canonical 6-helix bundle, like those reported for other coronaviruses, was revealed, with three HR1 helices forming the central coiled-coil core and three HR2 chains surrounding the core in the HR1 side grooves. This demonstrates that MERS-CoV utilizes a mechanism similar to other class I enveloped viruses for membrane fusion. With this notion, we further identified an HR2-based peptide that could potently inhibit MERS-CoV fusion and entry by using a pseudotyped-virus system. These results lay the ground for future inhibitory peptidic drug design.

	Structure of the fusion core and inhibition of fusion by a heptad-repeat peptide derived from the S protein of MERS-CoV.,Gao J, Lu G, Qi J, Li Y, Wu Y, Deng Y, Geng H, Li H, Wang Q, Xiao H, Tan W, Yan J, Gao GF J Virol. 2013 Sep 25. PMID:24067982<ref>PMID:24067982</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4mod" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Sandbox 3001\|Sandbox 3001]]		*[[Sandbox 3001\|Sandbox 3001]]
	*[[Spike protein\|Spike protein]]		*[[Spike protein\|Spike protein]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Deng, Y]]		[[Category: Deng Y]]
	[[Category: Gao~~, G F~~]]		[[Category: Gao GF]]
	[[Category: Gao, J]]		[[Category: Gao J]]
	[[Category: Geng, H]]		[[Category: Geng H]]
	[[Category: Li, Y]]		[[Category: Li Y]]
	[[Category: Lu, G]]		[[Category: Lu G]]
	[[Category: Qi, J]]		[[Category: Qi J]]
	[[Category: Tan, W]]		[[Category: Tan W]]
	[[Category: Wu, Y]]		[[Category: Wu Y]]
	[[Category: Xiao, H]]		[[Category: Xiao H]]
	[[Category: Yan, J]]		[[Category: Yan J]]
	~~[[Category: 6-helix bundle]]~~
	~~[[Category: Membrane fusion]]~~
	~~[[Category: Mers-cov]]~~
	~~[[Category: Spike]]~~
	~~[[Category: Trimer of hairpin]]~~
	~~[[Category: Viral envelope protein]]~~
	~~[[Category: Viral protein~~]]

OCA at 05:04, 25 August 2022

2022-08-25T05:04:14Z

← Older revision		Revision as of 05:04, 25 August 2022
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	<StructureSection load='4mod' size='340' side='right'caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4mod' size='340' side='right'caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MOD FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MOD FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnc\|1wnc]], [[1wdf\|1wdf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wnc\|1wnc]], [[1wdf\|1wdf]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [~~http~~://pdbe.org/4mod PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mod PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [https://pdbe.org/4mod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [https://www.ebi.ac.uk/pdbsum/4mod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 4mod" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4mod" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Sandbox 3001\|Sandbox 3001]]
			*[[Spike protein\|Spike protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:57, 14 August 2019

2019-08-14T17:57:23Z

← Older revision		Revision as of 17:57, 14 August 2019
Line 1:		Line 1:

	==Structure of the MERS-CoV fusion core==		==Structure of the MERS-CoV fusion core==
	<StructureSection load='4mod' size='340' side='right' caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4mod' size='340' side='right'caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOD FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOD FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Deng, Y]]		[[Category: Deng, Y]]
	[[Category: Gao, G F]]		[[Category: Gao, G F]]

OCA at 08:57, 3 August 2017

2017-08-03T08:57:11Z

← Older revision		Revision as of 08:57, 3 August 2017
Line 3:		Line 3:
	<StructureSection load='4mod' size='340' side='right' caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4mod' size='340' side='right' caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Human_betacoronavirus_2c_emc/2012 Human betacoronavirus 2c emc/2012]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOD FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOD FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnc\|1wnc]], [[1wdf\|1wdf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnc\|1wnc]], [[1wdf\|1wdf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spike, S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1235996 Human betacoronavirus 2c EMC/2012])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [http://pdbe.org/4mod PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [http://www.ebi.ac.uk/pdbsum/4mod PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [http://pdbe.org/4mod PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [http://www.ebi.ac.uk/pdbsum/4mod PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Human betacoronavirus 2c emc/2012]]~~
	[[Category: Deng, Y]]		[[Category: Deng, Y]]
	[[Category: Gao, G F]]		[[Category: Gao, G F]]

OCA at 07:33, 11 August 2016

2016-08-11T07:33:40Z

← Older revision		Revision as of 07:33, 11 August 2016
Line 1:		Line 1:

	==Structure of the MERS-CoV fusion core==		==Structure of the MERS-CoV fusion core==
	<StructureSection load='4mod' size='340' side='right' caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4mod' size='340' side='right' caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnc\|1wnc]], [[1wdf\|1wdf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnc\|1wnc]], [[1wdf\|1wdf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spike, S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1235996 Human betacoronavirus 2c EMC/2012])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spike, S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1235996 Human betacoronavirus 2c EMC/2012])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [http://www.ebi.ac.uk/pdbsum/4mod PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [http://pdbe.org/4mod PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [http://www.ebi.ac.uk/pdbsum/4mod PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mod ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4mod" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:47, 5 January 2015

2015-01-05T08:47:46Z

← Older revision		Revision as of 08:47, 5 January 2015
Line 1:		Line 1:
	~~{{STRUCTURE_4mod~~\| ~~PDB~~=4mod \| ~~SCENE~~= }}		==Structure of the MERS-CoV fusion core==
	===~~Structure~~ of the MERS-CoV fusion core~~===~~		<StructureSection load='4mod' size='340' side='right' caption='[[4mod]], [[Resolution\|resolution]] 1.90Å' scene=''>
	~~{{ABSTRACT_PUBMED_24067982}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4mod]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_betacoronavirus_2c_emc/2012 Human betacoronavirus 2c emc/2012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MOD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MOD FirstGlance]. <br>
			</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnc\|1wnc]], [[1wdf\|1wdf]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spike, S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1235996 Human betacoronavirus 2c EMC/2012])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mod RCSB], [http://www.ebi.ac.uk/pdbsum/4mod PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Middle East respiratory syndrome coronavirus (MERS-CoV) recently emerged as a severe worldwide public health concern. The virus is highly pathogenic, manifesting in infected patients with an approximately 50% fatality rate. It is known that the surface spike (S) proteins of coronaviruses mediate receptor recognition and membrane fusion, thereby playing an indispensable role in initiating infection. In this process, the heptad repeats 1 and 2 (HR1 and HR2) of the S protein assemble into a complex called the fusion core, which represents a key membrane fusion architecture. To date, however, the MERS-CoV fusion core remains uncharacterized thus far. Here, we performed a series of biochemical and biophysical analyses characterizing the HR1/HR2 complexes of this novel virus. The HR sequences were variably truncated and then connected with a flexible amino acid linker. In each case, the recombinant protein automatically assembled into a trimer in solution, displaying a typical alpha-helical structure. One of these trimers was successfully crystallized, and its structure was solved at a resolution of 1.9 A. A canonical 6-helix bundle, like those reported for other coronaviruses, was revealed, with three HR1 helices forming the central coiled-coil core and three HR2 chains surrounding the core in the HR1 side grooves. This demonstrates that MERS-CoV utilizes a mechanism similar to other class I enveloped viruses for membrane fusion. With this notion, we further identified an HR2-based peptide that could potently inhibit MERS-CoV fusion and entry by using a pseudotyped-virus system. These results lay the ground for future inhibitory peptidic drug design.

	~~==About this~~ Structure==		Structure of the fusion core and inhibition of fusion by a heptad-repeat peptide derived from the S protein of MERS-CoV.,Gao J, Lu G, Qi J, Li Y, Wu Y, Deng Y, Geng H, Li H, Wang Q, Xiao H, Tan W, Yan J, Gao GF J Virol. 2013 Sep 25. PMID:24067982<ref>PMID:24067982</ref>
	~~[[4mod]] is~~ a ~~2 chain structure. Full crystallographic information is available~~ from ~~[http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4MOD OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	<~~ref group="xtra">PMID:024067982<~~/~~ref~~><~~references group="xtra"~~/>~~<references~~/>		</div>
	[[Category: Deng, Y.]]		== References ==
	[[Category: Gao, G F.]]		<references/>
	[[Category: Gao, J.]]		__TOC__
	[[Category: Geng, H.]]		</StructureSection>
	[[Category: Li, Y.]]		[[Category: Human betacoronavirus 2c emc/2012]]
	[[Category: Lu, G.]]		[[Category: Deng, Y]]
	[[Category: Qi, J.]]		[[Category: Gao, G F]]
	[[Category: Tan, W.]]		[[Category: Gao, J]]
	[[Category: Wu, Y.]]		[[Category: Geng, H]]
	[[Category: Xiao, H.]]		[[Category: Li, Y]]
	[[Category: Yan, J.]]		[[Category: Lu, G]]
			[[Category: Qi, J]]
			[[Category: Tan, W]]
			[[Category: Wu, Y]]
			[[Category: Xiao, H]]
			[[Category: Yan, J]]
	[[Category: 6-helix bundle]]		[[Category: 6-helix bundle]]
	[[Category: Membrane fusion]]		[[Category: Membrane fusion]]

OCA at 06:28, 10 October 2013

2013-10-10T06:28:56Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{STRUCTURE_4mod|  PDB=4mod  |  SCENE=  }}
-The entry 4mod is ON HOLD  until Paper Publication
+==About this Structure==
-Authors: Gao, J., Lu, G., Qi, J., Li, Y., Wu, Y., Deng, Y., Geng, H., Xiao, H., Tan, W., Yan, J., Gao, G.F.
+==Reference==
+<ref group="xtra">PMID:024067982</ref><references group="xtra"/><references/>
-Description: Structure of the MERS-CoV fusion core
+[[Category: Deng, Y.]]

OCA at 06:51, 2 October 2013

2013-10-02T06:51:08Z

← Older revision		Revision as of 06:51, 2 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4mod is ON HOLD		The entry 4mod is ON HOLD until Paper Publication

	Authors: Gao, J., Lu, G., Qi, J., Li, Y., Wu, Y., Deng, Y., Geng, H., Xiao, H., Tan, W., Yan, J., Gao, G.F.		Authors: Gao, J., Lu, G., Qi, J., Li, Y., Wu, Y., Deng, Y., Geng, H., Xiao, H., Tan, W., Yan, J., Gao, G.F.

	Description: ~~structure~~ of the MERS-CoV fusion core		Description: Structure of the MERS-CoV fusion core

OCA: Protected "4mod" [edit=sysop:move=sysop]

2013-09-20T03:44:35Z

Protected "4mod" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:44, 20 September 2013
(No difference)