4nee - Revision history

OCA at 16:55, 20 September 2023

2023-09-20T16:55:16Z

← Older revision		Revision as of 16:55, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nee]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nee]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NEE FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [https://pdbe.org/4nee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [https://www.ebi.ac.uk/pdbsum/4nee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nee ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8841Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [https://pdbe.org/4nee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [https://www.ebi.ac.uk/pdbsum/4nee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nee ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:25, 11 January 2023

2023-01-11T08:25:01Z

← Older revision		Revision as of 08:25, 11 January 2023
Line 1:		Line 1:

	==crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef==		==crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef==
	<StructureSection load='4nee' size='340' side='right' caption='[[4nee]], [[Resolution\|resolution]] 2.88Å' scene=''>		<StructureSection load='4nee' size='340' side='right'caption='[[4nee]], [[Resolution\|resolution]] 2.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nee]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~9hiv1 9hiv1~~] and [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nee]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NEE FirstGlance]. <br>
	</td></tr>~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [https://pdbe.org/4nee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [https://www.ebi.ac.uk/pdbsum/4nee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nee ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ap2s1, Ap17, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Ap17, Ap2a2, Ap2s1, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), nef ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [~~http~~://pdbe.org/4nee PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4nee PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4nee ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/AP2S1_RAT AP2S1_RAT]] Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis (By similarity).<ref>PMID:14745134</ref> <ref>PMID:15473838</ref>		[https://www.uniprot.org/uniprot/AP2S1_RAT AP2S1_RAT] Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis (By similarity).<ref>PMID:14745134</ref> <ref>PMID:15473838</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 19:

	==See Also==		==See Also==
	*[[Adaptin\|Adaptin]]		*[[Adaptin 3D structures\|Adaptin 3D structures]]
			*[[Protein Nef\|Protein Nef]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Buffalo rat~~]]		[[Category: Human immunodeficiency virus 1]]
	[[Category: ~~Bonifacino, J S~~]]		[[Category: Large Structures]]
	[[Category: ~~Hurley, J H~~]]		[[Category: Rattus norvegicus]]
	[[Category: ~~Park, S Y~~]]		[[Category: Bonifacino JS]]
	[[Category: ~~Ren, X~~]]		[[Category: Hurley JH]]
	[[Category: ~~Cd4 downregulation~~]]		[[Category: Park SY]]
	[[Category: ~~Clathrin adaptor ap-2]]~~		[[Category: Ren X]]
	~~[[Category: Hiv-1 nef]]~~
	~~[[Category: Viral protein-protein transport complex~~]]

OCA at 22:07, 5 August 2016

2016-08-05T22:07:05Z

← Older revision		Revision as of 22:07, 5 August 2016
Line 1:		Line 1:

	==crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef==		==crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef==
	<StructureSection load='4nee' size='340' side='right' caption='[[4nee]], [[Resolution\|resolution]] 2.88Å' scene=''>		<StructureSection load='4nee' size='340' side='right' caption='[[4nee]], [[Resolution\|resolution]] 2.88Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ap2s1, Ap17, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Ap17, Ap2a2, Ap2s1, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), nef ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ap2s1, Ap17, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Ap17, Ap2a2, Ap2s1, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), nef ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [http://www.ebi.ac.uk/pdbsum/4nee PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [http://pdbe.org/4nee PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [http://www.ebi.ac.uk/pdbsum/4nee PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nee ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4nee" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Adaptin\|Adaptin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:15, 25 December 2014

2014-12-25T06:15:08Z

← Older revision		Revision as of 06:15, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nee]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nee]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NEE FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ap2s1, Ap17, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Ap17, Ap2a2, Ap2s1, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), nef ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ap2s1, Ap17, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Ap17, Ap2a2, Ap2s1, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), nef ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [http://www.ebi.ac.uk/pdbsum/4nee PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [http://www.ebi.ac.uk/pdbsum/4nee PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/AP2S1_RAT AP2S1_RAT]] Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis (By similarity).<ref>PMID:14745134</ref> <ref>PMID:15473838</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	How HIV-1 Nef hijacks the AP-2 clathrin adaptor to downregulate CD4.,Ren X, Park SY, Bonifacino JS, Hurley JH Elife. 2014;3:e01754. doi: 10.7554/eLife.01754. Epub 2014 Jan 1. PMID:24473078<ref>PMID:24473078</ref>		How HIV-1 Nef hijacks the AP-2 clathrin adaptor to downregulate CD4.,Ren X, Park SY, Bonifacino JS, Hurley JH Elife. 2014;3:e01754. doi: 10.7554/eLife.01754. Epub 2014 Jan 1. PMID:24473078<ref>PMID:24473078</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: 9hiv1]]~~
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Bonifacino, J S.]]		[[Category: Bonifacino, J S]]
	[[Category: Hurley, J H.]]		[[Category: Hurley, J H]]
	[[Category: Park, S Y.]]		[[Category: Park, S Y]]
	[[Category: Ren, X.]]		[[Category: Ren, X]]
	[[Category: Cd4 downregulation]]		[[Category: Cd4 downregulation]]
	[[Category: Clathrin adaptor ap-2]]		[[Category: Clathrin adaptor ap-2]]
	[[Category: Hiv-1 nef]]		[[Category: Hiv-1 nef]]
	[[Category: Viral protein-protein transport complex]]		[[Category: Viral protein-protein transport complex]]

OCA at 13:38, 18 May 2014

2014-05-18T13:38:22Z

← Older revision		Revision as of 13:38, 18 May 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4nee\| PDB=4nee \| SCENE= }}~~		==crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef==
	===crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef===		<StructureSection load='4nee' size='340' side='right' caption='[[4nee]], [[Resolution\|resolution]] 2.88Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4nee]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NEE FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ap2s1, Ap17, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Ap17, Ap2a2, Ap2s1, Claps2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), nef ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nee OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nee RCSB], [http://www.ebi.ac.uk/pdbsum/4nee PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Nef protein of HIV-1 downregulates the cell surface co-receptor CD4 by hijacking the clathrin adaptor complex AP-2. The structural basis for the hijacking of AP-2 by Nef is revealed by a 2.9 A crystal structure of Nef bound to the alpha and sigma2 subunits of AP-2. Nef binds to AP-2 via its central loop (residues 149-179) and its core. The determinants for Nef binding include residues that directly contact AP-2 and others that stabilize the binding-competent conformation of the central loop. Residues involved in both direct and indirect interactions are required for the binding of Nef to AP-2 and for downregulation of CD4. These results lead to a model for the docking of the full AP-2 tetramer to membranes as bound to Nef, such that the cytosolic tail of CD4 is situated to interact with its binding site on Nef. DOI: http://dx.doi.org/10.7554/eLife.01754.001.

	~~==Function==~~		How HIV-1 Nef hijacks the AP-2 clathrin adaptor to downregulate CD4.,Ren X, Park SY, Bonifacino JS, Hurley JH Elife. 2014;3:e01754. doi: 10.7554/eLife.01754. Epub 2014 Jan 1. PMID:24473078<ref>PMID:24473078</ref>
	~~[[http://www.uniprot.org/uniprot/AP2S1_RAT AP2S1_RAT]] Component of~~ the ~~adaptor protein complex 2 (~~AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin ~~lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated~~ adaptor ~~protein (AP) complexes which can bind directly to both the clathrin lattice and~~ to ~~the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation~~. ~~AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis~~. ~~AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface~~. ~~AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules~~. ~~AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway~~. ~~The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif~~. ~~May also play a role in extracellular calcium homeostasis (By similarity)~~.~~<ref>~~PMID:~~14745134</ref>~~ <ref>PMID:~~15473838~~</ref>

	~~==About this Structure==~~		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
	~~[[4nee]] is~~ a ~~12 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4NEE OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
			[[Category: 9hiv1]]
			[[Category: Buffalo rat]]
	[[Category: Bonifacino, J S.]]		[[Category: Bonifacino, J S.]]
	[[Category: Hurley, J H.]]		[[Category: Hurley, J H.]]

OCA at 07:51, 29 January 2014

2014-01-29T07:51:28Z

← Older revision		Revision as of 07:51, 29 January 2014
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	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_4nee\| PDB=4nee \| SCENE= }}
			===crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef===

	The ~~entry 4nee~~ is ~~ON HOLD~~ ~~until Paper Publication~~		==Function==
			[[http://www.uniprot.org/uniprot/AP2S1_RAT AP2S1_RAT]] Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis (By similarity).<ref>PMID:14745134</ref> <ref>PMID:15473838</ref>

	~~Authors~~: ~~Hurley, J.H., Bonifacino, J.S~~.~~, Ren, X~~.~~, Park, S~~.Y.		==About this Structure==
			[[4nee]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEE OCA].

	~~Description~~: ~~crystal structure of AP~~-2 ~~alpha/simga2 complex bound to HIV~~-1 ~~Nef~~		==Reference==
			<references group="xtra"/><references/>
			[[Category: Bonifacino, J S.]]
			[[Category: Hurley, J H.]]
			[[Category: Park, S Y.]]
			[[Category: Ren, X.]]
			[[Category: Cd4 downregulation]]
			[[Category: Clathrin adaptor ap-2]]
			[[Category: Hiv-1 nef]]
			[[Category: Viral protein-protein transport complex]]

OCA at 09:12, 8 January 2014

2014-01-08T09:12:25Z

← Older revision		Revision as of 09:12, 8 January 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4nee is ON HOLD		The entry 4nee is ON HOLD until Paper Publication

	Authors: Hurley, J.H., Bonifacino, J.S., Ren, X., Park, S.Y.		Authors: Hurley, J.H., Bonifacino, J.S., Ren, X., Park, S.Y.

	Description: crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef		Description: crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef

OCA at 13:03, 11 December 2013

2013-12-11T13:03:24Z

← Older revision		Revision as of 13:03, 11 December 2013
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	The entry 4nee is ON HOLD		The entry 4nee is ON HOLD

	Authors: Hurley, J. H., Bonifacino, J. S., Ren, X. , Park, S. Y.		Authors: Hurley, J.H., Bonifacino, J.S., Ren, X., Park, S.Y.

	Description: crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef		Description: crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef

OCA: Protected "4nee" [edit=sysop:move=sysop]

2013-11-13T07:45:24Z

Protected "4nee" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:45, 13 November 2013
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4nee is ON HOLD Authors: Hurley, J. H., Bonifacino, J. S., Ren, X. , Park, S. Y. Description: crystal structure of AP-2 alpha/simga2 complex bound ...

2013-11-13T07:45:24Z

New page: '''Unreleased structure''' The entry 4nee is ON HOLD Authors: Hurley, J. H., Bonifacino, J. S., Ren, X. , Park, S. Y. Description: crystal structure of AP-2 alpha/simga2 complex bound ...

New page

'''Unreleased structure'''

The entry 4nee is ON HOLD

Authors: Hurley, J. H., Bonifacino, J. S., Ren, X. , Park, S. Y.

Description: crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef