4o7d - Revision history

OCA at 23:04, 26 March 2025

2025-03-26T23:04:18Z

← Older revision		Revision as of 23:04, 26 March 2025
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7D FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ONL:5-OXO-L-NORLEUCINE'>ONL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ONL:5-OXO-L-NORLEUCINE'>ONL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7d OCA], [https://pdbe.org/4o7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7d RCSB], [https://www.ebi.ac.uk/pdbsum/4o7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7d OCA], [https://pdbe.org/4o7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7d RCSB], [https://www.ebi.ac.uk/pdbsum/4o7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7d ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:59, 18 January 2023

2023-01-18T08:59:03Z

← Older revision		Revision as of 08:59, 18 January 2023
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	==Crystal structure of human glutaminase in complex DON==		==Crystal structure of human glutaminase in complex DON==
	<StructureSection load='4o7d' size='340' side='right' caption='[[4o7d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4o7d' size='340' side='right'caption='[[4o7d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7d]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4O7D FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ONL:5-OXO-L-NORLEUCINE'>ONL</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ONL:5-OXO-L-NORLEUCINE'>ONL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7d OCA], [https://pdbe.org/4o7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7d RCSB], [https://www.ebi.ac.uk/pdbsum/4o7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7d ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4o7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7d OCA], [~~http~~://pdbe.org/4o7d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4o7d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4o7d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.		[https://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Glutaminase\|Glutaminase]]		*[[Glutaminase 3D structures\|Glutaminase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Glutaminase~~]]		[[Category: Homo sapiens]]
	[[Category: ~~Human~~]]		[[Category: Large Structures]]
	[[Category: Sivaraman, J]]		[[Category: Sivaraman J]]
	[[Category: Thangavelu, K]]		[[Category: Thangavelu K]]
	~~[[Category: Hydrolase~~]]

OCA at 01:48, 5 August 2016

2016-08-05T01:48:33Z

← Older revision		Revision as of 01:48, 5 August 2016
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	==Crystal structure of human glutaminase in complex DON==		==Crystal structure of human glutaminase in complex DON==
	<StructureSection load='4o7d' size='340' side='right' caption='[[4o7d]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4o7d' size='340' side='right' caption='[[4o7d]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o7d RCSB], [http://www.ebi.ac.uk/pdbsum/4o7d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7d OCA], [http://pdbe.org/4o7d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7d RCSB], [http://www.ebi.ac.uk/pdbsum/4o7d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4o7d" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Glutaminase\|Glutaminase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:49, 25 December 2014

2014-12-25T14:49:46Z

← Older revision		Revision as of 14:49, 25 December 2014
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	~~{{STRUCTURE_4o7d\| PDB=4o7d \| SCENE= }}~~		==Crystal structure of human glutaminase in complex DON==
	===Crystal structure of human glutaminase in complex DON===		<StructureSection load='4o7d' size='340' side='right' caption='[[4o7d]], [[Resolution\|resolution]] 2.30Å' scene=''>
	~~{{ABSTRACT_PUBMED_24451979}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4o7d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7D FirstGlance]. <br>
	==Function==		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ONL:5-OXO-L-NORLEUCINE'>ONL</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLS, GLS1, KIAA0838 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaminase Glutaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.2 3.5.1.2] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o7d RCSB], [http://www.ebi.ac.uk/pdbsum/4o7d PDBsum]</span></td></tr>
			</table>
			== Function ==
	[[http://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.		[[http://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Glutaminase is a metabolic enzyme responsible for glutaminolysis, a process harnessed by cancer cells to feed their accelerated growth and proliferation. Among the glutaminase isoforms, human kidney-type glutaminase (KGA) is often upregulated in cancer and is thus touted as an attractive drug target. Here we report the active site inhibition mechanism of KGA through the crystal structure of the catalytic domain of KGA (cKGA) in complex with 6-diazo-5-oxo-L-norleucine (DON), a substrate analogue of glutamine. DON covalently binds with the active site Ser286 and interacts with residues such as Tyr249, Asn335, Glu381, Asn388, Tyr414, Tyr466 and Val484. The nucleophilic attack of Ser286 sidechain on DON releases the diazo group (N2) from the inhibitor and results in the formation of an enzyme-inhibitor complex. Mutational studies confirmed the key role of these residues in the activity of KGA. This study will be important in the development of KGA active site inhibitors for therapeutic interventions.

	~~==About this Structure==~~		Structural basis for the active site inhibition mechanism of human kidney-type glutaminase (KGA).,Thangavelu K, Chong QY, Low BC, Sivaraman J Sci Rep. 2014 Jan 23;4:3827. doi: 10.1038/srep03827. PMID:24451979<ref>PMID:24451979</ref>
	~~[[4o7d]] is a 1 chain structure~~. ~~Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4O7D OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group="xtra">PMID:024451979</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Glutaminase]]		[[Category: Glutaminase]]
	[[Category: Sivaraman, J.]]		[[Category: Human]]
	[[Category: Thangavelu, K.]]		[[Category: Sivaraman, J]]
			[[Category: Thangavelu, K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:48, 16 April 2014

2014-04-16T09:48:04Z

← Older revision		Revision as of 09:48, 16 April 2014
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	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_4o7d\| PDB=4o7d \| SCENE= }}
			===Crystal structure of human glutaminase in complex DON===
			{{ABSTRACT_PUBMED_24451979}}

	~~The entry 4o7d is ON HOLD until Paper Publication~~		==Function==
			[[http://www.uniprot.org/uniprot/GLSK_HUMAN GLSK_HUMAN]] Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.

	~~Authors~~: ~~Thangavelu, K~~.~~, Sivaraman, J~~.		==About this Structure==
			[[4o7d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7D OCA].

	~~Description~~: ~~Crystal structure of human glutaminase in complex DON~~		==Reference==
			<ref group="xtra">PMID:024451979</ref><references group="xtra"/><references/>
			[[Category: Glutaminase]]
			[[Category: Sivaraman, J.]]
			[[Category: Thangavelu, K.]]
			[[Category: Hydrolase]]

OCA at 04:49, 13 February 2014

2014-02-13T04:49:39Z

← Older revision		Revision as of 04:49, 13 February 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4o7d is ON HOLD		The entry 4o7d is ON HOLD until Paper Publication

	Authors: Thangavelu, K., Sivaraman, J.		Authors: Thangavelu, K., Sivaraman, J.

	Description: Crystal structure of human glutaminase in complex DON		Description: Crystal structure of human glutaminase in complex DON

OCA at 06:46, 22 January 2014

2014-01-22T06:46:33Z

← Older revision		Revision as of 06:46, 22 January 2014
Line 3:		Line 3:
	The entry 4o7d is ON HOLD		The entry 4o7d is ON HOLD

	Authors: Thangavelu K, Sivaraman J		Authors: Thangavelu, K., Sivaraman, J.

	Description: Crystal structure of human glutaminase in complex DON		Description: Crystal structure of human glutaminase in complex DON

OCA: Protected "4o7d" [edit=sysop:move=sysop]

2014-01-08T09:26:09Z

Protected "4o7d" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:26, 8 January 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4o7d is ON HOLD Authors: Thangavelu K, Sivaraman J Description: Crystal structure of human glutaminase in complex DON

2014-01-08T09:26:07Z

New page: '''Unreleased structure''' The entry 4o7d is ON HOLD Authors: Thangavelu K, Sivaraman J Description: Crystal structure of human glutaminase in complex DON

New page

'''Unreleased structure'''

The entry 4o7d is ON HOLD

Authors: Thangavelu K, Sivaraman J

Description: Crystal structure of human glutaminase in complex DON