4o7j - Revision history

OCA at 08:57, 20 March 2024

2024-03-20T08:57:36Z

← Older revision		Revision as of 08:57, 20 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_sp._ATCC_39006 Serratia sp. ATCC 39006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7J FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_sp._ATCC_39006 Serratia sp. ATCC 39006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [https://pdbe.org/4o7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [https://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [https://pdbe.org/4o7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [https://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	In the Gram-negative enterobacterium Erwinia (Pectobacterium) and Serratia sp. ATCC 39006, intrinsic resistance to the carbapenem antibiotic 1-carbapen-2-em-3-carboxylic acid is mediated by the CarF and CarG proteins, by an unknown mechanism. Here, we report a high-resolution crystal structure for the Serratia sp. ATCC 39006 carbapenem resistance protein CarG. This structure of CarG is the first in the carbapenem intrinsic resistance (CIR) family of resistance proteins from carbapenem-producing bacteria. The crystal structure shows the protein to form a homodimer, in agreement with results from analytical gel filtration. The structure of CarG does not show homology with any known antibiotic resistance proteins nor does it belong to any well-characterised protein structural family. However, it is a close structural homologue of the bacterial inhibitor of invertebrate lysozyme, PliI-Ah, with some interesting structural variations, including the absence of the catalytic site responsible for lysozyme inhibition. Both proteins show a unique beta-sandwich fold with short terminal alpha-helices. The core of the protein is formed by stacked anti-parallel sheets that are individually very similar in the two proteins but differ in their packing interface, causing the splaying of the two sheets in CarG. Furthermore, a conserved cation binding site identified in CarG is absent from the homologue.

	Crystal structure of the carbapenem intrinsic resistance protein CarG.,Tichy EM, Luisi BF, Salmond GP J Mol Biol. 2014 May 1;426(9):1958-70. doi: 10.1016/j.jmb.2014.02.016. Epub 2014 , Feb 28. PMID:24583229<ref>PMID:24583229</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4o7j" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:59, 18 January 2023

2023-01-18T08:59:11Z

← Older revision		Revision as of 08:59, 18 January 2023
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	==Crystal structure of CarG==		==Crystal structure of CarG==
	<StructureSection load='4o7j' size='340' side='right' caption='[[4o7j]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4o7j' size='340' side='right'caption='[[4o7j]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sers3 Sers3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4O7J FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_sp._ATCC_39006 Serratia sp. ATCC 39006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CarG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104623 SERS3])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [https://pdbe.org/4o7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [https://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [~~http~~://pdbe.org/4o7j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Sers3~~]]		[[Category: Large Structures]]
	[[Category: ~~Luisi, B F]]~~		[[Category: Serratia sp. ATCC 39006]]
	~~[[Category: Salmond, G P~~.C]]		[[Category: Luisi BF]]
	[[Category: ~~Tichy, E M~~]]		[[Category: Salmond GPC]]
	[[Category: ~~Antibiotic resistance~~]]		[[Category: Tichy EM]]
	[[Category: ~~Carbapenem resistance]]~~
	~~[[Category: Lysozyme inhibitor structural homologue~~]]

OCA at 14:23, 15 November 2017

2017-11-15T14:23:25Z

← Older revision		Revision as of 14:23, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4o7j' size='340' side='right' caption='[[4o7j]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4o7j' size='340' side='right' caption='[[4o7j]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Serratia_sp._atcc_39006 Serratia sp. atcc 39006~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7J FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sers3 Sers3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CarG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104623 ~~Serratia sp. ATCC 39006~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CarG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104623 SERS3])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [http://pdbe.org/4o7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [http://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [http://pdbe.org/4o7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [http://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Serratia sp. atcc 39006~~]]		[[Category: Sers3]]
	[[Category: Luisi, B F]]		[[Category: Luisi, B F]]
	[[Category: Salmond, G P.C]]		[[Category: Salmond, G P.C]]

OCA at 14:49, 4 August 2016

2016-08-04T14:49:09Z

← Older revision		Revision as of 14:49, 4 August 2016
Line 1:		Line 1:

	==Crystal structure of CarG==		==Crystal structure of CarG==
	<StructureSection load='4o7j' size='340' side='right' caption='[[4o7j]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4o7j' size='340' side='right' caption='[[4o7j]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CarG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104623 Serratia sp. ATCC 39006])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CarG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104623 Serratia sp. ATCC 39006])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [http://www.ebi.ac.uk/pdbsum/4o7j PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [http://pdbe.org/4o7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [http://www.ebi.ac.uk/pdbsum/4o7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4o7j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:46, 24 December 2014

2014-12-24T10:46:09Z

← Older revision		Revision as of 10:46, 24 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_4o7j~~\| ~~PDB~~=4o7j \| ~~SCENE~~= }}		==Crystal structure of CarG==
	===~~Crystal structure of~~ CarG===		<StructureSection load='4o7j' size='340' side='right' caption='[[4o7j]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4o7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Serratia_sp._atcc_39006 Serratia sp. atcc 39006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7J FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CarG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104623 Serratia sp. ATCC 39006])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o7j RCSB], [http://www.ebi.ac.uk/pdbsum/4o7j PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			In the Gram-negative enterobacterium Erwinia (Pectobacterium) and Serratia sp. ATCC 39006, intrinsic resistance to the carbapenem antibiotic 1-carbapen-2-em-3-carboxylic acid is mediated by the CarF and CarG proteins, by an unknown mechanism. Here, we report a high-resolution crystal structure for the Serratia sp. ATCC 39006 carbapenem resistance protein CarG. This structure of CarG is the first in the carbapenem intrinsic resistance (CIR) family of resistance proteins from carbapenem-producing bacteria. The crystal structure shows the protein to form a homodimer, in agreement with results from analytical gel filtration. The structure of CarG does not show homology with any known antibiotic resistance proteins nor does it belong to any well-characterised protein structural family. However, it is a close structural homologue of the bacterial inhibitor of invertebrate lysozyme, PliI-Ah, with some interesting structural variations, including the absence of the catalytic site responsible for lysozyme inhibition. Both proteins show a unique beta-sandwich fold with short terminal alpha-helices. The core of the protein is formed by stacked anti-parallel sheets that are individually very similar in the two proteins but differ in their packing interface, causing the splaying of the two sheets in CarG. Furthermore, a conserved cation binding site identified in CarG is absent from the homologue.

	~~==About this Structure==~~		Crystal structure of the carbapenem intrinsic resistance protein CarG.,Tichy EM, Luisi BF, Salmond GP J Mol Biol. 2014 May 1;426(9):1958-70. doi: 10.1016/j.jmb.2014.02.016. Epub 2014 , Feb 28. PMID:24583229<ref>PMID:24583229</ref>
	~~[[4o7j]] is a 2 chain~~ structure. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin~~/~~ocashort?id=4O7J OCA~~].
	[[Category: Luisi, B F.]]		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: Salmond, G P.C.]]		</div>
	[[Category: Tichy, E M.]]		== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Serratia sp. atcc 39006]]
			[[Category: Luisi, B F]]
			[[Category: Salmond, G P.C]]
			[[Category: Tichy, E M]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Carbapenem resistance]]		[[Category: Carbapenem resistance]]
	[[Category: Lysozyme inhibitor structural homologue]]		[[Category: Lysozyme inhibitor structural homologue]]

OCA at 14:03, 12 March 2014

2014-03-12T14:03:27Z

← Older revision		Revision as of 14:03, 12 March 2014
Line 1:		Line 1:
	~~'''Unreleased~~ structure~~'''~~		{{STRUCTURE_4o7j\| PDB=4o7j \| SCENE= }}
			===Crystal structure of CarG===

	~~The entry~~ 4o7j is ~~ON HOLD until Paper Publication~~		==About this Structure==
			[[4o7j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7J OCA].
	~~Authors~~: ~~Tichy, E~~.M., Luisi, B.F., Salmond, G.P.C.		[[Category: Luisi, B F.]]
			[[Category: Salmond, G P.C.]]
	~~Description~~: ~~Crystal structure of CarG~~		[[Category: Tichy, E M.]]
			[[Category: Antibiotic resistance]]
			[[Category: Carbapenem resistance]]
			[[Category: Lysozyme inhibitor structural homologue]]

OCA at 07:05, 29 January 2014

2014-01-29T07:05:03Z

← Older revision		Revision as of 07:05, 29 January 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4o7j is ON HOLD		The entry 4o7j is ON HOLD until Paper Publication

	Authors: Tichy, E.M., Luisi, B.F., Salmond, G.P.C.		Authors: Tichy, E.M., Luisi, B.F., Salmond, G.P.C.

	Description: Crystal structure of CarG		Description: Crystal structure of CarG

OCA at 07:42, 15 January 2014

2014-01-15T07:42:18Z

← Older revision		Revision as of 07:42, 15 January 2014
Line 3:		Line 3:
	The entry 4o7j is ON HOLD		The entry 4o7j is ON HOLD

	Authors: Tichy, E. M., Luisi, B. F., Salmond, G. P. C.		Authors: Tichy, E.M., Luisi, B.F., Salmond, G.P.C.

	Description: CarG		Description: Crystal structure of CarG

OCA: Protected "4o7j" [edit=sysop:move=sysop]

2014-01-08T09:26:23Z

Protected "4o7j" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:26, 8 January 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4o7j is ON HOLD Authors: Tichy, E. M., Luisi, B. F., Salmond, G. P. C. Description: CarG

2014-01-08T09:26:22Z

New page: '''Unreleased structure''' The entry 4o7j is ON HOLD Authors: Tichy, E. M., Luisi, B. F., Salmond, G. P. C. Description: CarG

New page

'''Unreleased structure'''

The entry 4o7j is ON HOLD

Authors: Tichy, E. M., Luisi, B. F., Salmond, G. P. C.

Description: CarG