4o7u - Revision history

OCA at 12:38, 1 March 2024

2024-03-01T12:38:11Z

← Older revision		Revision as of 12:38, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7U FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SS7:4-{[(2S)-2-(1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL)PROPYL]OXY}-3,5-DIMETHYLPHENYL+ACETATE'>SS7</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SS7:4-{[(2S)-2-(1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL)PROPYL]OXY}-3,5-DIMETHYLPHENYL+ACETATE'>SS7</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [https://pdbe.org/4o7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [https://www.ebi.ac.uk/pdbsum/4o7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [https://pdbe.org/4o7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [https://www.ebi.ac.uk/pdbsum/4o7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]		[https://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Infections caused by Enterococcus faecalis (Ef) represent nowadays a relevant health problem. We selected Thymidylate synthase (TS) from this organism as a potential specific target for antibacterial therapy. We have previously demonstrated that species-specific inhibition of the protein can be achieved despite the relatively high structural similarity among bacterial TSs and human TS. We had previously obtained the EfTS crystal structure of the protein in complex with the metabolite 5-formyl-tetrahydrofolate (5-FTHF) suggesting the protein role as metabolite reservoir; however, protein-inhibitors complexes were still missing. In the present work we identified some inhibitors bearing the phthalimidic core from our in-house library and we performed crystallographic screening towards EfTS. We obtained two X-ray crystallographic structures: the first with a weak phthalimidic inhibitor bound in one subunit and 5-hydroxymethylene-6-hydrofolic acid (5-HMHF) in the other subunit; a second X-ray structure complex with methotrexate. The structural information achieved confirm the role of EfTS as an enzyme involved in the folate pool system and provide a structural basis for structure-based drug design.

	X-ray crystal structures of Enterococcus faecalis thymidylate synthase with folate binding site inhibitors.,Catalano A, Luciani R, Carocci A, Cortesi D, Pozzi C, Borsari C, Ferrari S, Mangani S Eur J Med Chem. 2016 Nov 10;123:649-64. doi: 10.1016/j.ejmech.2016.07.066. Epub, 2016 Jul 29. PMID:27517810<ref>PMID:27517810</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 4o7u" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Thymidylate synthase 3D structures\|Thymidylate synthase 3D structures]]		*[[Thymidylate synthase 3D structures\|Thymidylate synthase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:59, 18 January 2023

2023-01-18T08:59:36Z

← Older revision		Revision as of 08:59, 18 January 2023
Line 3:		Line 3:
	<StructureSection load='4o7u' size='340' side='right'caption='[[4o7u]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4o7u' size='340' side='right'caption='[[4o7u]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7u]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Entfa Entfa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4O7U FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O7U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SS7:4-{[(2S)-2-(1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL)PROPYL]OXY}-3,5-DIMETHYLPHENYL+ACETATE'>SS7</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SS7:4-{[(2S)-2-(1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL)PROPYL]OXY}-3,5-DIMETHYLPHENYL+ACETATE'>SS7</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uwl\|3uwl]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [https://pdbe.org/4o7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [https://www.ebi.ac.uk/pdbsum/4o7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7u ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thyA, EF_1576 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226185 ENTFA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [~~http~~://pdbe.org/4o7u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4o7u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA]] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]		[https://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
	*[[Thymidylate synthase\|Thymidylate synthase]]		*[[Thymidylate synthase 3D structures\|Thymidylate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Entfa~~]]		[[Category: Enterococcus faecalis V583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Thymidylate synthase]]~~		[[Category: Mangani S]]
	[[Category: Mangani, S]]		[[Category: Pozzi C]]
	[[Category: Pozzi, C]]
	~~[[Category: Folate binding]]~~
	~~[[Category: Transferase]]~~
	~~[[Category: Transferase-transferase inhibitor complex~~]]

OCA at 09:03, 10 April 2019

2019-04-10T09:03:28Z

← Older revision		Revision as of 09:03, 10 April 2019
Line 1:		Line 1:

	==Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7==		==Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7==
	<StructureSection load='4o7u' size='340' side='right' caption='[[4o7u]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4o7u' size='340' side='right'caption='[[4o7u]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o7u]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7U FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o7u]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Entfa Entfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SS7:4-{[(2S)-2-(1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL)PROPYL]OXY}-3,5-DIMETHYLPHENYL+ACETATE'>SS7</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SS7:4-{[(2S)-2-(1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL)PROPYL]OXY}-3,5-DIMETHYLPHENYL+ACETATE'>SS7</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uwl\|3uwl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uwl\|3uwl]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thyA, EF_1576 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226185 ENTFA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [http://pdbe.org/4o7u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [http://www.ebi.ac.uk/pdbsum/4o7u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [http://pdbe.org/4o7u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [http://www.ebi.ac.uk/pdbsum/4o7u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7u ProSAT]</span></td></tr>
Line 27:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Entfa]]
			[[Category: Large Structures]]
	[[Category: Thymidylate synthase]]		[[Category: Thymidylate synthase]]
	[[Category: Mangani, S]]		[[Category: Mangani, S]]

OCA at 14:12, 5 October 2016

2016-10-05T14:12:53Z

← Older revision		Revision as of 14:12, 5 October 2016
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA]] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]		[[http://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA]] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Infections caused by Enterococcus faecalis (Ef) represent nowadays a relevant health problem. We selected Thymidylate synthase (TS) from this organism as a potential specific target for antibacterial therapy. We have previously demonstrated that species-specific inhibition of the protein can be achieved despite the relatively high structural similarity among bacterial TSs and human TS. We had previously obtained the EfTS crystal structure of the protein in complex with the metabolite 5-formyl-tetrahydrofolate (5-FTHF) suggesting the protein role as metabolite reservoir; however, protein-inhibitors complexes were still missing. In the present work we identified some inhibitors bearing the phthalimidic core from our in-house library and we performed crystallographic screening towards EfTS. We obtained two X-ray crystallographic structures: the first with a weak phthalimidic inhibitor bound in one subunit and 5-hydroxymethylene-6-hydrofolic acid (5-HMHF) in the other subunit; a second X-ray structure complex with methotrexate. The structural information achieved confirm the role of EfTS as an enzyme involved in the folate pool system and provide a structural basis for structure-based drug design.

			X-ray crystal structures of Enterococcus faecalis thymidylate synthase with folate binding site inhibitors.,Catalano A, Luciani R, Carocci A, Cortesi D, Pozzi C, Borsari C, Ferrari S, Mangani S Eur J Med Chem. 2016 Nov 10;123:649-64. doi: 10.1016/j.ejmech.2016.07.066. Epub, 2016 Jul 29. PMID:27517810<ref>PMID:27517810</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 4o7u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Thymidylate synthase\|Thymidylate synthase]]		*[[Thymidylate synthase\|Thymidylate synthase]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:18, 4 August 2016

2016-08-04T08:18:28Z

← Older revision		Revision as of 08:18, 4 August 2016
Line 1:		Line 1:

	==Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7==		==Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7==
	<StructureSection load='4o7u' size='340' side='right' caption='[[4o7u]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4o7u' size='340' side='right' caption='[[4o7u]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uwl\|3uwl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uwl\|3uwl]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [http://www.ebi.ac.uk/pdbsum/4o7u PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [http://pdbe.org/4o7u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [http://www.ebi.ac.uk/pdbsum/4o7u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o7u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA]] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]		[[http://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA]] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]

			==See Also==
			*[[Thymidylate synthase\|Thymidylate synthase]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:50, 31 December 2014

2014-12-31T14:50:45Z

← Older revision		Revision as of 14:50, 31 December 2014
Line 1:		Line 1:
	'''~~Unreleased structure~~'''		==Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7==
			<StructureSection load='4o7u' size='340' side='right' caption='[[4o7u]], [[Resolution\|resolution]] 2.40Å' scene=''>
	~~The entry~~ 4o7u is ~~ON HOLD until Paper Publication~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4o7u]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O7U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O7U FirstGlance]. <br>
	~~Authors~~: ~~Mangani~~, S., ~~Pozzi~~, C.		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SS7:4-{[(2S)-2-(1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL)PROPYL]OXY}-3,5-DIMETHYLPHENYL+ACETATE'>SS7</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3uwl\|3uwl]]</td></tr>
	~~Description~~: ~~Etherocomplex of Enteroccocus faecalis thymidylate~~ synthase with 5-~~hydroxymethilene~~-6-~~hydrofolic acid and~~ the ~~phtalimidic inhibitor SS7~~		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o7u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o7u RCSB], [http://www.ebi.ac.uk/pdbsum/4o7u PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/TYSY_ENTFA TYSY_ENTFA]] Provides the sole de novo source of dTMP for DNA biosynthesis.[HAMAP-Rule:MF_00008]
			__TOC__
			</StructureSection>
			[[Category: Thymidylate synthase]]
	[[Category: Mangani, S]]		[[Category: Mangani, S]]
	[[Category: Pozzi, C]]		[[Category: Pozzi, C]]
			[[Category: Folate binding]]
			[[Category: Transferase]]
			[[Category: Transferase-transferase inhibitor complex]]

OCA at 14:18, 24 December 2014

2014-12-24T14:18:17Z

← Older revision		Revision as of 14:18, 24 December 2014
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	Description: Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7		Description: Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7
			[[Category: Unreleased Structures]]
			[[Category: Mangani, S]]
			[[Category: Pozzi, C]]

OCA at 06:37, 9 July 2014

2014-07-09T06:37:48Z

← Older revision		Revision as of 06:37, 9 July 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4o7u is ON HOLD		The entry 4o7u is ON HOLD until Paper Publication

	Authors: Mangani, S., Pozzi, C.		Authors: Mangani, S., Pozzi, C.

	Description: Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7		Description: Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7

OCA: Protected "4o7u" [edit=sysop:move=sysop]

2014-01-08T09:26:54Z

Protected "4o7u" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:26, 8 January 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4o7u is ON HOLD Authors: Mangani, S., Pozzi, C. Description: Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-...

2014-01-08T09:26:53Z

New page: '''Unreleased structure''' The entry 4o7u is ON HOLD Authors: Mangani, S., Pozzi, C. Description: Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-...

New page

'''Unreleased structure'''

The entry 4o7u is ON HOLD

Authors: Mangani, S., Pozzi, C.

Description: Etherocomplex of Enteroccocus faecalis thymidylate synthase with 5-hydroxymethilene-6-hydrofolic acid and the phtalimidic inhibitor SS7