4ogs - Revision history

OCA at 07:16, 27 November 2024

2024-11-27T07:16:18Z

← Older revision		Revision as of 07:16, 27 November 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OGS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OGS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.21Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [https://pdbe.org/4ogs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [https://www.ebi.ac.uk/pdbsum/4ogs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [https://pdbe.org/4ogs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [https://www.ebi.ac.uk/pdbsum/4ogs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogs ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:24, 25 January 2023

2023-01-25T07:24:31Z

← Older revision		Revision as of 07:24, 25 January 2023
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	<StructureSection load='4ogs' size='340' side='right'caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='4ogs' size='340' side='right'caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OGS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OGS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>~~</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [https://pdbe.org/4ogs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [https://www.ebi.ac.uk/pdbsum/4ogs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogs ProSAT]</span></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>~~
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [~~http~~://pdbe.org/4ogs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ogs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.		[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Aeqvi~~]]		[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Remington~~, S J~~]]		[[Category: Remington SJ]]
	[[Category: Saif, M]]		[[Category: Saif M]]
	~~[[Category: Beta-can]]~~
	~~[[Category: Fluorescent protein~~]]

OCA at 07:53, 17 April 2019

2019-04-17T07:53:50Z

← Older revision		Revision as of 07:53, 17 April 2019
Line 1:		Line 1:

	==Crystal structure of GFP S205A/T203V at 2.2 A resolution==		==Crystal structure of GFP S205A/T203V at 2.2 A resolution==
	<StructureSection load='4ogs' size='340' side='right' caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='4ogs' size='340' side='right'caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OGS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OGS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://pdbe.org/4ogs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://pdbe.org/4ogs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogs ProSAT]</span></td></tr>
	</table>		</table>
Line 27:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Aeqvi]]
			[[Category: Large Structures]]
	[[Category: Remington, S J]]		[[Category: Remington, S J]]
	[[Category: Saif, M]]		[[Category: Saif, M]]
	[[Category: Beta-can]]		[[Category: Beta-can]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]

OCA at 20:26, 5 August 2016

2016-08-05T20:26:06Z

← Older revision		Revision as of 20:26, 5 August 2016
Line 1:		Line 1:

	==Crystal structure of GFP S205A/T203V at 2.2 A resolution==		==Crystal structure of GFP S205A/T203V at 2.2 A resolution==
	<StructureSection load='4ogs' size='340' side='right' caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='4ogs' size='340' side='right' caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://pdbe.org/4ogs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ogs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4ogs" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:36, 25 December 2014

2014-12-25T05:36:05Z

← Older revision		Revision as of 05:36, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OGS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OGS FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Remington, S J.]]		[[Category: Remington, S J]]
	[[Category: Saif, M.]]		[[Category: Saif, M]]
	[[Category: Beta-can]]		[[Category: Beta-can]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]

OCA at 05:28, 18 June 2014

2014-06-18T05:28:35Z

← Older revision		Revision as of 05:28, 18 June 2014
Line 2:		Line 2:
	<StructureSection load='4ogs' size='340' side='right' caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='4ogs' size='340' side='right' caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. <br>		<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OGS FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum]</span></td></tr>
	<table>		<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Mutations near the fluorescing chromophore of the green fluorescent protein (GFP) have direct effects on the absorption and emission spectra. Some mutants have significant band shifts and most of the mutants exhibit a loss of fluorescence intensity. In this study we continue our investigation of the factors controlling the excited state proton transfer (PT) process of GFP, in particular to study the effects of modifications to the key side chain Ser205 in wt-GFP, proposed to participate in the proton wire. To this aim we combined mutagenesis, X-ray crystallography, steady-state spectroscopy, time-resolved emission spectroscopy and all-atom explicit molecular dynamics (MD) simulations to study the double mutant T203V/S205A. Our results show that while in the previously described GFP double mutant T203V/S205V the PT process does not occur, in the T203V/S205A mutant the PT process does occur, but with a 350 times slower rate than in wild-type GFP (wt-GFP). Furthermore, the kinetic isotope effect in the GFP double mutant T203V/S205A is twice smaller than in the wt-GFP and in the GFP single mutant S205V, which forms a novel PT pathway. On the other hand, the crystal structure of GFP T203V/S205A does not reveal a viable proton transfer pathway. To explain PT in GFP T203V/S205A, we argue on the basis of the MD simulations for an alternative, novel proton-wire pathway which involves the phenol group of the chromophore and water molecules infrequently entering from the bulk. This alternative pathway may explain the dramatically slow PT in the GFP double mutant T203V/S205A compared to wt-GFP.

			Insight into the structure and the mechanism of the slow proton transfer in the GFP double mutant T203V/S205A.,Wineman-Fisher V, Simkovitch R, Shomer S, Gepshtein R, Huppert D, Saif M, Kallio K, Remington SJ, Miller Y Phys Chem Chem Phys. 2014 Jun 21;16(23):11211-23. doi: 10.1039/c4cp00311j. Epub, 2014 Apr 28. PMID:24776960<ref>PMID:24776960</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:27, 14 May 2014

2014-05-14T07:27:16Z

← Older revision		Revision as of 07:27, 14 May 2014
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	'''~~Unreleased~~ structure'''		==Crystal structure of GFP S205A/T203V at 2.2 A resolution==
			<StructureSection load='4ogs' size='340' side='right' caption='[[4ogs]], [[Resolution\|resolution]] 2.21Å' scene=''>
	~~The entry~~ 4ogs ~~is ON HOLD~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[4ogs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OGS OCA]. <br>
	~~Authors~~: Remington, S.J., Saif, M.		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
			<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	~~Description~~: ~~Crystal structure of GFP S205A/T203V at 2.2 A resolution~~		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qle\|2qle]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ogs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ogs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ogs RCSB], [http://www.ebi.ac.uk/pdbsum/4ogs PDBsum]</span></td></tr>
			<table>
			__TOC__
			</StructureSection>
			[[Category: Remington, S J.]]
			[[Category: Saif, M.]]
			[[Category: Beta-can]]
			[[Category: Fluorescent protein]]

OCA: Protected "4ogs" [edit=sysop:move=sysop]

2014-01-22T06:49:11Z

Protected "4ogs" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:49, 22 January 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4ogs is ON HOLD Authors: Remington, S.J., Saif, M. Description: Crystal structure of GFP S205A/T203V at 2.2 A resolution

2014-01-22T06:49:10Z

New page: '''Unreleased structure''' The entry 4ogs is ON HOLD Authors: Remington, S.J., Saif, M. Description: Crystal structure of GFP S205A/T203V at 2.2 A resolution

New page

'''Unreleased structure'''

The entry 4ogs is ON HOLD

Authors: Remington, S.J., Saif, M.

Description: Crystal structure of GFP S205A/T203V at 2.2 A resolution