4phi - Revision history

OCA at 11:16, 6 November 2024

2024-11-06T11:16:41Z

← Older revision		Revision as of 11:16, 6 November 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4phi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PHI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4phi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PHI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA~~</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW~~</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.811Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4phi OCA], [https://pdbe.org/4phi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4phi RCSB], [https://www.ebi.ac.uk/pdbsum/4phi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4phi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4phi OCA], [https://pdbe.org/4phi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4phi RCSB], [https://www.ebi.ac.uk/pdbsum/4phi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4phi ProSAT]</span></td></tr>
	</table>		</table>

OCA at 13:03, 1 February 2023

2023-02-01T13:03:23Z

← Older revision		Revision as of 13:03, 1 February 2023
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	<StructureSection load='4phi' size='340' side='right'caption='[[4phi]], [[Resolution\|resolution]] 1.81Å' scene=''>		<StructureSection load='4phi' size='340' side='right'caption='[[4phi]], [[Resolution\|resolution]] 1.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4phi]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PHI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PHI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4phi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PHI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4phi OCA], [https://pdbe.org/4phi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4phi RCSB], [https://www.ebi.ac.uk/pdbsum/4phi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4phi ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4phi OCA], [~~http~~://pdbe.org/4phi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4phi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4phi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4phi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Lysozyme]]~~		[[Category: Al-Oweini R]]
	[[Category: Al-Oweini, R]]		[[Category: Bijelic A]]
	[[Category: Bijelic, A]]		[[Category: Kortz U]]
	[[Category: Kortz, U]]		[[Category: Mauracher SG]]
	[[Category: Mauracher~~, S G~~]]		[[Category: Molitor C]]
	[[Category: Molitor, C]]		[[Category: Rompel A]]
	[[Category: Rompel, A]]
	~~[[Category: Anderson evans type]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Polyoxometalate~~]]

OCA at 08:16, 17 April 2019

2019-04-17T08:16:11Z

← Older revision		Revision as of 08:16, 17 April 2019
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	==Crystal structure of HEWL with hexatungstotellurate(VI)==		==Crystal structure of HEWL with hexatungstotellurate(VI)==
	<StructureSection load='4phi' size='340' side='right' caption='[[4phi]], [[Resolution\|resolution]] 1.81Å' scene=''>		<StructureSection load='4phi' size='340' side='right'caption='[[4phi]], [[Resolution\|resolution]] 1.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4phi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PHI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4phi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PHI FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 4phi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4phi" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
			[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Al-Oweini, R]]		[[Category: Al-Oweini, R]]

OCA at 16:41, 11 August 2016

2016-08-11T16:41:53Z

← Older revision		Revision as of 16:41, 11 August 2016
Line 1:		Line 1:

	==Crystal structure of HEWL with hexatungstotellurate(VI)==		==Crystal structure of HEWL with hexatungstotellurate(VI)==
	<StructureSection load='4phi' size='340' side='right' caption='[[4phi]], [[Resolution\|resolution]] 1.81Å' scene=''>		<StructureSection load='4phi' size='340' side='right' caption='[[4phi]], [[Resolution\|resolution]] 1.81Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4phi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4phi RCSB], [http://www.ebi.ac.uk/pdbsum/4phi PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4phi OCA], [http://pdbe.org/4phi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4phi RCSB], [http://www.ebi.ac.uk/pdbsum/4phi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4phi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4phi" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:32, 14 January 2015

2015-01-14T13:32:48Z

← Older revision		Revision as of 13:32, 14 January 2015
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	'''~~Unreleased~~ structure'''		==Crystal structure of HEWL with hexatungstotellurate(VI)==
			<StructureSection load='4phi' size='340' side='right' caption='[[4phi]], [[Resolution\|resolution]] 1.81Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4phi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PHI FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TEW:6-TUNGSTOTELLURATE(VI)'>TEW</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4phi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4phi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4phi RCSB], [http://www.ebi.ac.uk/pdbsum/4phi PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			As synchrotron radiation becomes more intense, detectors become faster and structure-solving software becomes more elaborate, obtaining single crystals suitable for data collection is now the bottleneck in macromolecular crystallography. Hence, there is a need for novel and advanced crystallisation agents with the ability to crystallise proteins that are otherwise challenging. Here, an Anderson-Evans-type polyoxometalate (POM), specifically Na6 [TeW6 O24 ]22 H2 O (TEW), is employed as a crystallisation additive. Its effects on protein crystallisation are demonstrated with hen egg-white lysozyme (HEWL), which co-crystallises with TEW in the vicinity (or within) the liquid-liquid phase separation (LLPS) region. The X-ray structure (PDB ID: 4PHI) determination revealed that TEW molecules are part of the crystal lattice, thus demonstrating specific binding to HEWL with electrostatic interactions and hydrogen bonds. The negatively charged TEW polyoxotungstate binds to sites with a positive electrostatic potential located between two (or more) symmetry-related protein chains. Thus, TEW facilitates the formation of protein-protein interfaces of otherwise repulsive surfaces, and thereby the realisation of a stable crystal lattice. In addition to retaining the isomorphicity of the protein structure, the anomalous scattering of the POMs was used for macromolecular phasing. The results suggest that hexatungstotellurate(VI) has great potential as a crystallisation additive to promote both protein crystallisation and structure elucidation.

	~~The entry 4phi is ON HOLD until Paper Publication~~		Hen Egg-White Lysozyme Crystallisation: Protein Stacking and Structure Stability Enhanced by a Tellurium(VI)-Centred Polyoxotungstate.,Bijelic A, Molitor C, Mauracher SG, Al-Oweini R, Kortz U, Rompel A Chembiochem. 2015 Jan 19;16(2):233-41. doi: 10.1002/cbic.201402597. Epub 2014 Dec, 17. PMID:25521080<ref>PMID:25521080</ref>

	~~Authors: Bijelic~~, ~~A., Molitor, C~~.~~, Mauracher,~~ S.G., Al-Oweini, R~~., Kortz, U., Rompel, A.~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~Description: Crystal structure of HEWL with hexatungstotellurate(VI)~~		== References ==
	[[Category: ~~Unreleased Structures~~]]		<references/>
			__TOC__
			</StructureSection>
			[[Category: Gallus gallus]]
			[[Category: Lysozyme]]
			[[Category: Al-Oweini, R]]
			[[Category: Bijelic, A]]
	[[Category: Kortz, U]]		[[Category: Kortz, U]]
	[[Category: Mauracher, S.G]]		[[Category: Mauracher, S G]]
	~~[[Category: Bijelic, A]]~~
	~~[[Category: Al-Oweini, R~~]]
	[[Category: Molitor, C]]		[[Category: Molitor, C]]
	[[Category: Rompel, A]]		[[Category: Rompel, A]]
			[[Category: Anderson evans type]]
			[[Category: Hydrolase]]
			[[Category: Polyoxometalate]]

OCA at 15:02, 24 December 2014

2014-12-24T15:02:52Z

← Older revision		Revision as of 15:02, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of HEWL with hexatungstotellurate(VI)		Description: Crystal structure of HEWL with hexatungstotellurate(VI)
			[[Category: Unreleased Structures]]
			[[Category: Kortz, U]]
			[[Category: Mauracher, S.G]]
			[[Category: Bijelic, A]]
			[[Category: Al-Oweini, R]]
			[[Category: Molitor, C]]
			[[Category: Rompel, A]]

OCA at 08:03, 21 May 2014

2014-05-21T08:03:55Z

← Older revision		Revision as of 08:03, 21 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4phi is ON HOLD		The entry 4phi is ON HOLD until Paper Publication

	Authors: Bijelic, A., Molitor, C., Mauracher, S.G., Al-Oweini, R., Kortz, U., Rompel, A.		Authors: Bijelic, A., Molitor, C., Mauracher, S.G., Al-Oweini, R., Kortz, U., Rompel, A.

	Description: Crystal structure of HEWL with hexatungstotellurate(VI)		Description: Crystal structure of HEWL with hexatungstotellurate(VI)

OCA: Protected "4phi" [edit=sysop:move=sysop]

2014-05-14T07:08:20Z

Protected "4phi" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:08, 14 May 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4phi is ON HOLD Authors: Bijelic, A., Molitor, C., Mauracher, S.G., Al-Oweini, R., Kortz, U., Rompel, A. Description: Crystal structure of HEWL wit...

2014-05-14T07:08:19Z

New page: '''Unreleased structure''' The entry 4phi is ON HOLD Authors: Bijelic, A., Molitor, C., Mauracher, S.G., Al-Oweini, R., Kortz, U., Rompel, A. Description: Crystal structure of HEWL wit...

New page

'''Unreleased structure'''

The entry 4phi is ON HOLD

Authors: Bijelic, A., Molitor, C., Mauracher, S.G., Al-Oweini, R., Kortz, U., Rompel, A.

Description: Crystal structure of HEWL with hexatungstotellurate(VI)