4toc - Revision history

OCA at 11:50, 13 August 2026

2026-08-13T11:50:53Z

← Older revision		Revision as of 11:50, 13 August 2026
Line 9:		Line 9:
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~Q9HY79_PSEAE Q9HY79_PSEAE~~] ~~Iron~~-storage protein (~~By similarity~~). ~~Iron-storage protein, whose~~ ferroxidase center binds Fe(2+) ~~ions~~, oxidizes ~~them by~~ dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (~~By similarity~~).~~[PIRNR~~:~~PIRNR002560]~~		[https://www.uniprot.org/uniprot/BFRB_PSEAE BFRB_PSEAE] The major iron-storage protein, part of the heterooligomeric bacterioferritin (BFR) complex. The ferroxidase center binds Fe(2+), oxidizes it using dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the BFR protein shell. Can store up to 600 iron atoms per bacterioferritin protein molecule (PubMed:19575528, PubMed:20067302, PubMed:25640193, PubMed:26368531). In iron-sufficient conditions (10 uM Fe(2+)) iron accumulates in BFR until about 12 hours, when it starts to deplete; stored iron is no longer detectable by 24 hours growth, iron is mobilized from the BFR as levels drop in the growth media (PubMed:28318006). Iron release from the BFR requires ferredoxin NADP reductase (FPR) and bacterioferritin-associated ferredoxin (Bfd) (PubMed:19575528, PubMed:22812654, PubMed:26368531). Reduction of the BfrB heme group occurs in the presence of Bfd, strongly suggesting that the BfrB-Bfd complex allows heme to mediate electron transfer from FPR to the Fe(3+) iron core in the BFR shell prior to its release as Fe(2+) (PubMed:19575528, PubMed:22812654, PubMed:26368531).<ref>PMID:19575528</ref> <ref>PMID:20067302</ref> <ref>PMID:22812654</ref> <ref>PMID:25640193</ref> <ref>PMID:26368531</ref> <ref>PMID:28318006</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:21, 27 September 2023

2023-09-27T07:21:44Z

← Older revision		Revision as of 07:21, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TOC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TOC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [https://pdbe.org/4toc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [https://www.ebi.ac.uk/pdbsum/4toc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4toc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [https://pdbe.org/4toc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [https://www.ebi.ac.uk/pdbsum/4toc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4toc ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:47, 15 March 2023

2023-03-15T10:47:48Z

← Older revision		Revision as of 10:47, 15 March 2023
Line 3:		Line 3:
	<StructureSection load='4toc' size='340' side='right'caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4toc' size='340' side='right'caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TOC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TOC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4to9\|4to9]], [[4toa\|4toa]], [[4tob\|4tob]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]]</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [https://pdbe.org/4toc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [https://www.ebi.ac.uk/pdbsum/4toc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4toc ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [~~http~~://pdbe.org/4toc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4toc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4toc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]		[https://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Ferroxidase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Pseae~~]]		[[Category: Pseudomonas aeruginosa PAO1]]
	[[Category: Battaile~~, K P~~]]		[[Category: Battaile KP]]
	[[Category: Eshelman, K]]		[[Category: Eshelman K]]
	[[Category: Kumar, R]]		[[Category: Kumar R]]
	[[Category: Lovell, S]]		[[Category: Lovell S]]
	[[Category: Rivera, M]]		[[Category: Rivera M]]
	[[Category: Yao, H]]		[[Category: Yao H]]
	~~[[Category: Electron transport]]~~
	~~[[Category: Iron binding]]~~
	~~[[Category: Iron mobilization]]~~
	~~[[Category: Iron storage]]~~
	~~[[Category: Oxidoreductase~~]]

OCA at 07:17, 27 November 2019

2019-11-27T07:17:17Z

← Older revision		Revision as of 07:17, 27 November 2019
Line 1:		Line 1:

	==2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa==		==2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa==
	<StructureSection load='4toc' size='340' side='right' caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4toc' size='340' side='right'caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TOC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TOC FirstGlance]. <br>
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4toc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4toc" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Ferritin 3D structures\|Ferritin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
			[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]

OCA at 14:56, 15 November 2017

2017-11-15T14:56:56Z

← Older revision		Revision as of 14:56, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='4toc' size='340' side='right' caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4toc' size='340' side='right' caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TOC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TOC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4to9\|4to9]], [[4toa\|4toa]], [[4tob\|4tob]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4to9\|4to9]], [[4toa\|4toa]], [[4tob\|4tob]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfrB, PA3531 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [http://pdbe.org/4toc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [http://www.ebi.ac.uk/pdbsum/4toc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4toc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [http://pdbe.org/4toc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [http://www.ebi.ac.uk/pdbsum/4toc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4toc ProSAT]</span></td></tr>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
			[[Category: Pseae]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Eshelman, K]]		[[Category: Eshelman, K]]

OCA at 01:09, 19 January 2017

2017-01-19T01:09:37Z

← Older revision		Revision as of 01:09, 19 January 2017
Line 1:		Line 1:

	==2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa==		==2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa==
	<StructureSection load='4toc' size='340' side='right' caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='4toc' size='340' side='right' caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4to9\|4to9]], [[4toa\|4toa]], [[4tob\|4tob]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4to9\|4to9]], [[4toa\|4toa]], [[4tob\|4tob]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [http://www.ebi.ac.uk/pdbsum/4toc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [http://pdbe.org/4toc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [http://www.ebi.ac.uk/pdbsum/4toc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4toc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4toc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:48, 12 February 2015

2015-02-12T11:48:33Z

← Older revision		Revision as of 11:48, 12 February 2015
Line 1:		Line 1:
	'''~~Unreleased~~ structure'''		==2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa==
			<StructureSection load='4toc' size='340' side='right' caption='[[4toc]], [[Resolution\|resolution]] 2.25Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4toc]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TOC FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4to9\|4to9]], [[4toa\|4toa]], [[4tob\|4tob]], [[4tod\|4tod]], [[4toe\|4toe]], [[4tof\|4tof]], [[4tog\|4tog]], [[4toh\|4toh]]</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4toc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4toc RCSB], [http://www.ebi.ac.uk/pdbsum/4toc PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q9HY79_PSEAE Q9HY79_PSEAE]] Iron-storage protein (By similarity). Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex (By similarity).[PIRNR:PIRNR002560]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			X-ray crystallography, molecular dynamics (MD) simulations and biochemistry were utilized to investigate the effect of introducing hydrophobic interactions in the 4-fold (N148L and Q151L) and B-pores (D34F) of Pseudomonas aeruginosa bacterioferritin B (BfrB) on BfrB function. The structures show only local structural perturbations and confirm the anticipated hydrophobic interactions. Surprisingly, structures obtained after soaking crystals in Fe2+-containing crystallization solution revealed that although iron loads into the ferroxidase centers of the mutants, the side chains of ferroxidase ligands E51 and H130 do not reorganize to bind the iron ions, as is seen in the wt BfrB structures. Similar experiments with a double mutant (C89S/K96C) prepared to introduce changes outside the pores show competent ferroxidase centers that function akin to those in wt BfrB. MD simulations comparing wt BfrB with the D34F and N148L mutants show that the mutants exhibit significantly reduced flexibility, and reveal a network of concerted motions linking ferroxidase centers and 4-fold and B-pores, which are important for imparting ferroxidase centers in BfrB with the required flexibility to function efficiently. In agreement, the efficiency of Fe2+ oxidation and uptake of the 4-fold and B-pore mutants in solution is significantly compromised relative to wt or C89S/K96C BfrB. Finally, our structures show a large number of previously unknown iron binding sites in the interior cavity and B-pores of BfrB, which reveal in unprecedented detail conduits followed by iron and phosphate ions across the BfrB shell, as well as paths in the interior cavity that may facilitate nucleation of the iron phosphate mineral.

	~~The entry 4toc is ON HOLD until Paper Publication~~		Concerted Motions Networking Pores and Distant Ferroxidase Centers Enable Bacterioferritin Function and Iron Traffic.,Yao H, Rui H, Kumar R, Eshelman K, Lovell S, Battaile KP, Im W, Rivera M Biochemistry. 2015 Jan 31. PMID:25640193<ref>PMID:25640193</ref>

	~~Authors: Lovell~~, S.~~, Battaile, K.P., Yao, H., Kumar, R., Eshelman, K., Rivera, M~~.		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~Description~~: ~~2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa~~		== References ==
	[[Category: ~~Unreleased Structures~~]]		<references/>
	[[Category: ~~Rivera~~, M]]		__TOC__
			</StructureSection>
			[[Category: Ferroxidase]]
			[[Category: Battaile, K P]]
			[[Category: Eshelman, K]]
	[[Category: Kumar, R]]		[[Category: Kumar, R]]
	~~[[Category: Battaile, K.P]]~~
	[[Category: Lovell, S]]		[[Category: Lovell, S]]
	[[Category: ~~Eshelman~~, K]]		[[Category: Rivera, M]]
	[[Category: Yao, H]]		[[Category: Yao, H]]
			[[Category: Electron transport]]
			[[Category: Iron binding]]
			[[Category: Iron mobilization]]
			[[Category: Iron storage]]
			[[Category: Oxidoreductase]]

OCA at 17:44, 24 December 2014

2014-12-24T17:44:46Z

← Older revision		Revision as of 17:44, 24 December 2014
Line 6:		Line 6:

	Description: 2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa		Description: 2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa
			[[Category: Unreleased Structures]]
			[[Category: Rivera, M]]
			[[Category: Kumar, R]]
			[[Category: Battaile, K.P]]
			[[Category: Lovell, S]]
			[[Category: Eshelman, K]]
			[[Category: Yao, H]]

OCA at 06:01, 25 June 2014

2014-06-25T06:01:28Z

← Older revision		Revision as of 06:01, 25 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 4toc is ON HOLD		The entry 4toc is ON HOLD until Paper Publication

	Authors: Lovell, ~~Scott~~, Battaile, ~~Kevin~~ P., Yao, ~~Huili~~, Kumar, ~~Ritesh~~, Eshelman, ~~Kate~~, Rivera, ~~Mario~~		Authors: Lovell, S., Battaile, K.P., Yao, H., Kumar, R., Eshelman, K., Rivera, M.

	Description: 2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa		Description: 2.25A resolution structure of Iron Bound BfrB (Q151L) from Pseudomonas aeruginosa

OCA: Protected "4toc" [edit=sysop:move=sysop]

2014-06-11T08:16:10Z

Protected "4toc" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:16, 11 June 2014
(No difference)