4tpb - Revision history

OCA: Redirecting to 4u26

2014-12-10T15:52:49Z

Redirecting to 4u26

← Older revision		Revision as of 15:52, 10 December 2014
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	~~==Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 50S subunit of the second 70S ribosome with dalfopristin and quinupristin bound.==~~		#REDIRECT [[4u26]] This PDB entry is obsolete and replaced by 4u26
	~~<StructureSection load='4tpb' size='340' side='right' caption='~~[[~~4tpb]], [[Resolution\|resolution]] 2.80Å' scene=''>~~
	~~== Structural highlights ==~~
	~~<table><tr><td colspan='2'>[[4tpb~~]] is ~~a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]~~ and [http://en.wikipedia.org/wiki/Escherichia_coli_str._k-12_substr._mds42 Escherichia coli str. k-12 substr. mds42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TPB FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOL:5-(2-DIETHYLAMINO-ETHANESULFONYL)-21-HYDROXY-10-ISOPROPYL-11,19-DIMETHYL-9,26-DIOXA-3,15,28-TRIAZA-TRICYCLO[23.2.1.00,255]OCTACOSA-1(27),12,17,19,25(28)-PENTAENE-2,8,14,23-TETRAONE'>DOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=004:(2S)-AMINO(PHENYL)ETHANOIC+ACID'>004</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=MHT:(3S)-3-(METHYLSULFANYL)-1-AZABICYCLO[2.2.2]OCTANE'>MHT</scene>, <scene name='pdbligand=MHU:4-N,N-(DIMETHYLAMINO)-L-PHENYLALANINE'>MHU</scene>, <scene name='pdbligand=MHV:4-OXO-L-PIPECOLIC+ACID'>MHV</scene>, <scene name='pdbligand=MHW:3-HYDROXYPICOLINIC+ACID'>MHW</scene></td></tr>
	~~<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tp8\|4tp8]], [[4tp9\|4tp9]], [[4tpa\|4tpa]]</td></tr>~~
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tpb RCSB], [http://www.ebi.ac.uk/pdbsum/4tpb PDBsum]</span></td></tr>
	~~<table>~~
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Streptogramin antibiotics are divided into type A and B streptogramins, which in combination can act synergistically. We compared the molecular interactions of the streptogramin combinations Synercid (type A: dalfopristin, type B: quinupristin) and NXL 103 (type A: flopristin, type B: linopristin) with the Escherichia coli 70S ribosome by x-ray crystallography. We further analyzed the activity of the streptogramin components individually and in combination. Streptogramin A and B components in Synercid and NXL 103 exhibit synergistic antimicrobial activity against certain pathogenic bacteria. However, in transcription-coupled translation assays, only combinations that include dalfopristin, the streptogramin A component of Synercid, show synergy. Notably, the diethylaminoethylsulfonyl group in dalfopristin reduces its activity, but is the basis for synergy in transcription-coupled translation assays before its rapid hydrolysis from the depsipeptide core. Replacement of the diethylaminoethylsulfonyl group in dalfopristin by a non-hydrolyzable group may therefore be beneficial for synergy. The absence of general streptogramin synergy in transcription-coupled translation assays suggests that synergistic antimicrobial activity of streptogramins can occur independently of streptogramin effects on translation.

	Synergy of streptogramin antibiotics occurs independently of their effects on translation.,Noeske J, Huang J, Olivier NB, Giacobbe RA, Zambrowski M, Cate JH Antimicrob Agents Chemother. 2014 Jun 23. pii: AAC.03389-14. PMID:24957822<ref>PMID:24957822</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~== References ==~~
	~~<references/>~~
	~~__TOC__~~
	~~</StructureSection>~~
	~~[[Category: Escherichia coli]]~~
	~~[[Category: Escherichia coli str. k-12 substr. mds42]]~~
	~~[[Category: Cate, J H.D.]]~~
	~~[[Category: Giacobbe, R A.]]~~
	~~[[Category: Huang, J.]]~~
	~~[[Category: Noeske, J.]]~~
	~~[[Category: Olivier, N B.]]~~
	~~[[Category: Zambrowski, M.]]~~
	~~[[Category: 23]]~~
	~~[[Category: 50]]~~
	~~[[Category: 70]]~~
	~~[[Category: Antibiotic]]~~
	~~[[Category: Exit]]~~
	~~[[Category: Peptidyl]]~~
	~~[[Category: Protein biosynthesis]]~~
	~~[[Category: Ribosomal subunit]]~~
	~~[[Category: Ribosome]]~~
	~~[[Category: Rna]]~~
	~~[[Category: Streptogramin]]~~
	~~[[Category: Transfer]]~~
	~~[[Category: Trna]]~~

OCA at 07:42, 30 July 2014

2014-07-30T07:42:45Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 50S subunit of the second 70S ribosome with dalfopristin and quinupristin bound.==
-The entry 4tpb is ON HOLD
+Synergy of streptogramin antibiotics occurs independently of their effects on translation.,Noeske J, Huang J, Olivier NB, Giacobbe RA, Zambrowski M, Cate JH Antimicrob Agents Chemother. 2014 Jun 23. pii: AAC.03389-14. PMID:24957822<ref>PMID:24957822</ref>
-Authors: Noeske, J., Huang, J., Olivier, N.B., Giacobbe, R.A., Zambrowski, M., Cate, J.H.D.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 50S subunit of the second 70S ribosome with dalfopristin and quinupristin bound.
+== References ==

OCA: Protected "4tpb" [edit=sysop:move=sysop]

2014-06-18T06:45:50Z

Protected "4tpb" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:45, 18 June 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4tpb is ON HOLD Authors: Noeske, J., Huang, J., Olivier, N.B., Giacobbe, R.A., Zambrowski, M., Cate, J.H.D. Description: Crystal structure of the E...

2014-06-18T06:45:49Z

New page: '''Unreleased structure''' The entry 4tpb is ON HOLD Authors: Noeske, J., Huang, J., Olivier, N.B., Giacobbe, R.A., Zambrowski, M., Cate, J.H.D. Description: Crystal structure of the E...

New page

'''Unreleased structure'''

The entry 4tpb is ON HOLD

Authors: Noeske, J., Huang, J., Olivier, N.B., Giacobbe, R.A., Zambrowski, M., Cate, J.H.D.

Description: Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin. This file contains the 50S subunit of the second 70S ribosome with dalfopristin and quinupristin bound.