4ulp - Revision history

OCA: Redirecting to 4u50

2014-12-10T15:57:14Z

Redirecting to 4u50

← Older revision		Revision as of 15:57, 10 December 2014
Line 1:		Line 1:
	~~==Crystal structure of Verrucarin bound to the yeast 80S ribosome==~~		#REDIRECT [[4u50]] This PDB entry is obsolete and replaced by 4u50
	~~<StructureSection load='4ulp' size='340' side='right' caption='[[4ulp]], [[Resolution\|resolution]] 3.20Å' scene=''>~~
	~~== Structural highlights ==~~
	<table><tr><td colspan='2'>[[4ulp]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_atcc_204508_/_s288c Saccharomyces cerevisiae atcc 204508 / s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ULP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ULP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OHX:OSMIUM+(III)+HEXAMMINE'>OHX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ulq\|4ulq]], [[4ulr\|4ulr]], [[4uls\|4uls]], [[4ult\|4ult]]</td></tr>~~
	~~<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>~~[~~http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ulp FirstGlance],~~ [~~http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ulp OCA~~]~~, [http://www.rcsb.org/pdb/explore.do?structureId=4ulp RCSB], [http://www.ebi.ac.uk/pdbsum/4ulp PDBsum~~]~~</span></td></tr>~~
	~~</table>~~
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The ribosome is a molecular machine responsible for protein synthesis and a major target for small-molecule inhibitors. Compared to the wealth of structural information available on ribosome-targeting antibiotics in bacteria, our understanding of the binding mode of ribosome inhibitors in eukaryotes is currently limited. Here we used X-ray crystallography to determine 16 high-resolution structures of 80S ribosomes from Saccharomyces cerevisiae in complexes with 12 eukaryote-specific and 4 broad-spectrum inhibitors. All inhibitors were found associated with messenger RNA and transfer RNA binding sites. In combination with kinetic experiments, the structures suggest a model for the action of cycloheximide and lactimidomycin, which explains why lactimidomycin, the larger compound, specifically targets the first elongation cycle. The study defines common principles of targeting and resistance, provides insights into translation inhibitor mode of action and ~~reveals the structural determinants responsible for species selectivity which could guide future drug development.~~

	Structural basis for the inhibition of the eukaryotic ribosome.,Garreau de Loubresse N, Prokhorova I, Holtkamp W, Rodnina MV, Yusupova G, Yusupov M Nature. 2014 Sep 25;513(7519):517-22. doi: 10.1038/nature13737. Epub 2014 Sep 10. PMID:25209664<ref>PMID:25209664</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~== References ==~~
	~~<references/>~~
	~~__TOC__~~
	~~</StructureSection>~~
	~~[[Category: Saccharomyces cerevisiae atcc 204508 / s288c]]~~
	~~[[Category: Loubresse, N Garreau de.]]~~
	~~[[Category: Prokhorova, I.]]~~
	~~[[Category: Yusupov, M.]]~~
	~~[[Category: Yusupova, G.]]~~
	~~[[Category: 40]]~~
	~~[[Category: 60]]~~
	~~[[Category: 80]]~~
	~~[[Category: Antibiotic]]~~
	~~[[Category: Eukaryote]]~~
	~~[[Category: Inhibitor]]~~
	~~[[Category: Ribosome]]~~
	~~[[Category: Rna-protein complex]]~~
	~~[[Category: Translation]]~~

OCA: Protected "4ulp" [edit=sysop:move=sysop]

2014-10-22T12:28:28Z

Protected "4ulp" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:28, 22 October 2014
(No difference)

OCA: New page: ==Crystal structure of Verrucarin bound to the yeast 80S ribosome==
2014-10-22T12:28:26Z

New page: ==Crystal structure of Verrucarin bound to the yeast 80S ribosome== <StructureSection load='4ulp' size='340' side='right' caption='4ulp, resolution 3.20Å' scene=''...

New page
==Crystal structure of Verrucarin bound to the yeast 80S ribosome==
<StructureSection load='4ulp' size='340' side='right' caption='[[4ulp]], [[Resolution|resolution]] 3.20Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ulp]] is a 35 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_atcc_204508_/_s288c Saccharomyces cerevisiae atcc 204508 / s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ULP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ULP FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OHX:OSMIUM+(III)+HEXAMMINE'>OHX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ulq|4ulq]], [[4ulr|4ulr]], [[4uls|4uls]], [[4ult|4ult]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ulp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ulp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ulp RCSB], [http://www.ebi.ac.uk/pdbsum/4ulp PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The ribosome is a molecular machine responsible for protein synthesis and a major target for small-molecule inhibitors. Compared to the wealth of structural information available on ribosome-targeting antibiotics in bacteria, our understanding of the binding mode of ribosome inhibitors in eukaryotes is currently limited. Here we used X-ray crystallography to determine 16 high-resolution structures of 80S ribosomes from Saccharomyces cerevisiae in complexes with 12 eukaryote-specific and 4 broad-spectrum inhibitors. All inhibitors were found associated with messenger RNA and transfer RNA binding sites. In combination with kinetic experiments, the structures suggest a model for the action of cycloheximide and lactimidomycin, which explains why lactimidomycin, the larger compound, specifically targets the first elongation cycle. The study defines common principles of targeting and resistance, provides insights into translation inhibitor mode of action and reveals the structural determinants responsible for species selectivity which could guide future drug development.

Structural basis for the inhibition of the eukaryotic ribosome.,Garreau de Loubresse N, Prokhorova I, Holtkamp W, Rodnina MV, Yusupova G, Yusupov M Nature. 2014 Sep 25;513(7519):517-22. doi: 10.1038/nature13737. Epub 2014 Sep 10. PMID:25209664<ref>PMID:25209664</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Saccharomyces cerevisiae atcc 204508 / s288c]]
[[Category: Loubresse, N Garreau de.]]
[[Category: Prokhorova, I.]]
[[Category: Yusupov, M.]]
[[Category: Yusupova, G.]]
[[Category: 40]]
[[Category: 60]]
[[Category: 80]]
[[Category: Antibiotic]]
[[Category: Eukaryote]]
[[Category: Inhibitor]]
[[Category: Ribosome]]
[[Category: Rna-protein complex]]
[[Category: Translation]]