4whe - Revision history

OCA at 11:27, 9 May 2024

2024-05-09T11:27:41Z

← Older revision		Revision as of 11:27, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WHE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WHE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [https://pdbe.org/4whe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [https://www.ebi.ac.uk/pdbsum/4whe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4whe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [https://pdbe.org/4whe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [https://www.ebi.ac.uk/pdbsum/4whe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4whe ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:08, 7 April 2023

2023-04-07T07:08:35Z

← Older revision		Revision as of 07:08, 7 April 2023
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	<StructureSection load='4whe' size='340' side='right'caption='[[4whe]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4whe' size='340' side='right'caption='[[4whe]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WHE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WHE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pspA, Z2482, ECs1881 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [https://pdbe.org/4whe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [https://www.ebi.ac.uk/pdbsum/4whe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4whe ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [~~http~~://pdbe.org/4whe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4whe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4whe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/~~PSPA_ECO57 PSPA_ECO57]~~] The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation of the psp-specific transcriptional activator PspF. Is also required for membrane integrity, efficient translocation and maintenance of the proton motive force ~~(By similarity)~~.		[https://www.uniprot.org/uniprot/PSPA_ECOLI PSPA_ECOLI] The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation of the psp-specific transcriptional activator PspF. Is also required for membrane integrity, efficient translocation and maintenance of the proton motive force.<ref>PMID:10629175</ref> <ref>PMID:15485810</ref> <ref>PMID:1712397</ref> <ref>PMID:19804784</ref> <ref>PMID:8598199</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Ecoli~~]]		[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brueser, T]]		[[Category: Brueser T]]
	[[Category: Osadnik, H]]		[[Category: Osadnik H]]
	[[Category: Parthier, C]]		[[Category: Parthier C]]
	[[Category: Schoepfel, M]]		[[Category: Schoepfel M]]
	[[Category: Stubbs~~, M T]]~~		[[Category: Stubbs MT]]
	~~[[Category: Aaa+ protein regulation]]~~
	~~[[Category: Coiled-coil]]~~
	~~[[Category: Phage shock response]]~~
	~~[[Category: Pspa/im30 family]]~~
	~~[[Category: Sigma 54 promoter]]~~
	~~[[Category: Signaling protein]]~~
	~~[[Category: Stress inducible psp system]]~~
	~~[[Category: Transcription initiation]]~~
	~~[[Category: Transcriptional regulation~~]]

OCA at 09:09, 23 May 2019

2019-05-23T09:09:31Z

← Older revision		Revision as of 09:09, 23 May 2019
Line 1:		Line 1:

	==Crystal structure of E. coli phage shock protein A (PspA 1-144)==		==Crystal structure of E. coli phage shock protein A (PspA 1-144)==
	<StructureSection load='4whe' size='340' side='right' caption='[[4whe]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4whe' size='340' side='right'caption='[[4whe]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [http://pdbe.org/4whe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [http://www.ebi.ac.uk/pdbsum/4whe PDBsum]</span></td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pspA, Z2482, ECs1881 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [http://pdbe.org/4whe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [http://www.ebi.ac.uk/pdbsum/4whe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4whe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Ecoli]]
			[[Category: Large Structures]]
	[[Category: Brueser, T]]		[[Category: Brueser, T]]
	[[Category: Osadnik, H]]		[[Category: Osadnik, H]]

OCA at 22:09, 30 November 2015

2015-11-30T22:09:01Z

← Older revision		Revision as of 22:09, 30 November 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [http://www.ebi.ac.uk/pdbsum/4whe PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [http://pdbe.org/4whe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [http://www.ebi.ac.uk/pdbsum/4whe PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 4whe" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:26, 2 September 2015

2015-09-02T12:26:54Z

← Older revision		Revision as of 12:26, 2 September 2015
Line 1:		Line 1:
	'''~~Unreleased~~ structure'''		==Crystal structure of E. coli phage shock protein A (PspA 1-144)==
			<StructureSection load='4whe' size='340' side='right' caption='[[4whe]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[4whe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WHE FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4whe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4whe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4whe RCSB], [http://www.ebi.ac.uk/pdbsum/4whe PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PSPA_ECO57 PSPA_ECO57]] The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation of the psp-specific transcriptional activator PspF. Is also required for membrane integrity, efficient translocation and maintenance of the proton motive force (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Phage shock protein A (PspA) belongs to the highy conserved PspA/IM30 family and is a key component of the stress inducible Psp system in Escherichia coli. One of its central roles is the regulatory interaction with the transcriptional activator of this system, the sigma54 enhancer binding protein PspF, a member of the AAA+ protein family. The PspA/F regulatory system has been intensively studied and serves as a paradigm for AAA+ enzyme regulation by trans-acting factors. However, the molecular mechanism of how exactly PspA controls the activity of PspF and hence sigma54 -dependent expression of the psp genes is still unclear. To approach this question, we identified the minimal PspF-interacting domain of PspA, solved its structure, determined its affinity to PspF and the dissociation kinetics, identified residues that are potentially important for PspF regulation and analyzed effects of their mutation on PspF in vivo and in vitro. Our data indicate that several characteristics of AAA+ regulation in the PspA.F complex resemble those of the AAA+ unfoldase ClpB, with both proteins being regulated by a structurally highly conserved coiled-coil domain. The convergent evolution of both regulatory domains points to a general mechanism to control AAA+ activity for divergent physiological tasks via coiled-coil domains.

	The ~~entry 4whe is ON HOLD until Paper Publication~~		The PspF-binding domain PspA and the PspA.F complex - New insights into the coiled-coil dependent regulation of AAA+ proteins.,Osadnik H, Schopfel M, Heidrich E, Mehner D, Lilie H, Parthier C, Risselada HJ, Grubmuller H, Stubbs MT, Bruser T Mol Microbiol. 2015 Aug 3. doi: 10.1111/mmi.13154. PMID:26235546<ref>PMID:26235546</ref>

	~~Authors: Parthier~~, C.~~, Schoepfel, M~~.~~, Stubbs, M~~.~~T., Osadnik, H.,~~ Brueser, T.		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~Description:~~		== References ==
	[[Category: ~~Unreleased Structures~~]]		<references/>
			__TOC__
			</StructureSection>
			[[Category: Brueser, T]]
			[[Category: Osadnik, H]]
	[[Category: Parthier, C]]		[[Category: Parthier, C]]
	[[Category: Schoepfel, M]]		[[Category: Schoepfel, M]]
	[[Category: Stubbs, M.T]]		[[Category: Stubbs, M T]]
	[[Category: ~~Brueser, T~~]]		[[Category: Aaa+ protein regulation]]
	[[Category: ~~Osadnik, H~~]]		[[Category: Coiled-coil]]
			[[Category: Phage shock response]]
			[[Category: Pspa/im30 family]]
			[[Category: Sigma 54 promoter]]
			[[Category: Signaling protein]]
			[[Category: Stress inducible psp system]]
			[[Category: Transcription initiation]]
			[[Category: Transcriptional regulation]]

OCA at 19:30, 24 December 2014

2014-12-24T19:30:14Z

← Older revision		Revision as of 19:30, 24 December 2014
Line 5:		Line 5:
	Authors: Parthier, C., Schoepfel, M., Stubbs, M.T., Osadnik, H., Brueser, T.		Authors: Parthier, C., Schoepfel, M., Stubbs, M.T., Osadnik, H., Brueser, T.

	Description:		Description:
			[[Category: Unreleased Structures]]
			[[Category: Parthier, C]]
			[[Category: Schoepfel, M]]
			[[Category: Stubbs, M.T]]
			[[Category: Brueser, T]]
			[[Category: Osadnik, H]]

OCA: Protected "4whe" [edit=sysop:move=sysop]

2014-10-01T23:33:10Z

Protected "4whe" [edit=sysop:move=sysop]

← Older revision	Revision as of 23:33, 1 October 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4whe is ON HOLD until Paper Publication Authors: Parthier, C., Schoepfel, M., Stubbs, M.T., Osadnik, H., Brueser, T. Description:

2014-10-01T23:33:09Z

New page: '''Unreleased structure''' The entry 4whe is ON HOLD until Paper Publication Authors: Parthier, C., Schoepfel, M., Stubbs, M.T., Osadnik, H., Brueser, T. Description:

New page

'''Unreleased structure'''

The entry 4whe is ON HOLD until Paper Publication

Authors: Parthier, C., Schoepfel, M., Stubbs, M.T., Osadnik, H., Brueser, T.

Description: