4wtb - Revision history

OCA at 07:36, 27 September 2023

2023-09-27T07:36:29Z

← Older revision		Revision as of 07:36, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wtb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WTB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wtb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WTB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.16Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtb OCA], [https://pdbe.org/4wtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wtb RCSB], [https://www.ebi.ac.uk/pdbsum/4wtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtb OCA], [https://pdbe.org/4wtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wtb RCSB], [https://www.ebi.ac.uk/pdbsum/4wtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtb ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:25, 7 April 2023

2023-04-07T07:25:36Z

← Older revision		Revision as of 07:25, 7 April 2023
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	<StructureSection load='4wtb' size='340' side='right'caption='[[4wtb]], [[Resolution\|resolution]] 2.16Å' scene=''>		<StructureSection load='4wtb' size='340' side='right'caption='[[4wtb]], [[Resolution\|resolution]] 2.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wtb]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WTB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wtb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WTB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i3h\|3i3h]], [[3iq3\|3iq3]], [[3hzw\|3hzw]], [[3i03\|3i03]], [[3qnl\|3qnl]]~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtb OCA], [https://pdbe.org/4wtb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wtb RCSB], [https://www.ebi.ac.uk/pdbsum/4wtb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtb ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wtb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wtb OCA], [~~http~~://pdbe.org/4wtb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wtb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wtb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wtb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/~~PA2B1_BOTJR PA2B1_BOTJR]~~] Snake venom phospholipase A2 homolog that lacks enzymatic activity. In ~~vivo~~, induces ~~muscle necrosis~~, ~~accompanied by polymorphonuclear cell infiltration~~, and ~~edema~~ in the ~~mouse paw~~. ~~Damages~~ artificial and myoblast membranes by a calcium-independent mechanism~~. Has~~ bactericidal activity.<ref>PMID:~~3176051~~</ref> <ref>PMID:~~11018293~~</ref> <ref>PMID:~~11829743~~</ref> <ref>PMID:~~12079495~~</ref> <ref>PMID:17346668</ref> <ref>PMID:18160090</ref> <ref>PMID:~~17157889~~</ref>		[https://www.uniprot.org/uniprot/PA2H1_BOTJR PA2H1_BOTJR] Snake venom phospholipase A2 homolog that lacks enzymatic activity. Shows local myotoxic activity (PubMed:11018293, PubMed:12079495, PubMed:31906173). Induces inflammation, since it induces edema and leukocytes infiltration (PubMed:11018293, PubMed:31906173). In addition, it induces NLRP3 NLRP3, ASC (PYCARD), caspase-1 (CASP1), and IL-1beta (IL1B) gene expression in the gastrocnemius muscle, showing that it is able to activate NLRP3 inflammasome (PubMed:31906173). It also damages artificial and myoblast membranes by a calcium-independent mechanism, has bactericidal activity, and induces neuromuscular blockade (PubMed:27531710). A model of myotoxic mechanism has been proposed: an apo Lys49-PLA2 is activated by the entrance of a hydrophobic molecule (e.g. fatty acid) at the hydrophobic channel of the protein leading to a reorientation of a monomer (PubMed:27531710) (By similarity). This reorientation causes a transition between 'inactive' to 'active' states, causing alignment of C-terminal and membrane-docking sites (MDoS) side-by-side and putting the membrane-disruption sites (MDiS) in the same plane, exposed to solvent and in a symmetric position for both monomers (PubMed:27531710) (By similarity). The MDoS region stabilizes the toxin on membrane by the interaction of charged residues with phospholipid head groups (PubMed:27531710) (By similarity). Subsequently, the MDiS region destabilizes the membrane with penetration of hydrophobic residues (PubMed:27531710) (By similarity). This insertion causes a disorganization of the membrane, allowing an uncontrolled influx of ions (i.e. calcium and sodium), and eventually triggering irreversible intracellular alterations and cell death (PubMed:27531710) (By similarity).[UniProtKB:I6L8L6]<ref>PMID:11018293</ref> <ref>PMID:11829743</ref> <ref>PMID:12079495</ref> <ref>PMID:17157889</ref> <ref>PMID:17346668</ref> <ref>PMID:18160090</ref> <ref>PMID:27531710</ref> <ref>PMID:3176051</ref> <ref>PMID:31906173</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4wtb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4wtb" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Phospholipase A2 homolog\|Phospholipase A2 homolog]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Bothrops jararacussu]]		[[Category: Bothrops jararacussu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Borges~~, R J~~]]		[[Category: Borges RJ]]
	[[Category: Fontes~~, M R.M]]~~		[[Category: Fontes MRM]]
	~~[[Category: Bthtx-i]]~~
	~~[[Category: Svpla2-like inhibitor]]~~
	~~[[Category: Toxin~~]]

OCA at 06:18, 17 April 2019

2019-04-17T06:18:17Z

← Older revision		Revision as of 06:18, 17 April 2019
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	==BthTX-I, a svPLA2s-like toxin, complexed with zinc ions==		==BthTX-I, a svPLA2s-like toxin, complexed with zinc ions==
	<StructureSection load='4wtb' size='340' side='right' caption='[[4wtb]], [[Resolution\|resolution]] 2.16Å' scene=''>		<StructureSection load='4wtb' size='340' side='right'caption='[[4wtb]], [[Resolution\|resolution]] 2.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wtb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WTB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wtb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WTB FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Bothrops jararacussu]]		[[Category: Bothrops jararacussu]]
			[[Category: Large Structures]]
	[[Category: Borges, R J]]		[[Category: Borges, R J]]
	[[Category: Fontes, M R.M]]		[[Category: Fontes, M R.M]]

OCA at 09:03, 17 May 2017

2017-05-17T09:03:07Z

@@ Line 1: / Line 1: @@
-The entry 4wtb is ON HOLD
+==BthTX-I, a svPLA2s-like toxin, complexed with zinc ions==
-Authors: Borges, R.J., Fontes, M.R.M.
+Functional and structural studies of a Phospholipase A2-like protein complexed to zinc ions: Insights on its myotoxicity and inhibition mechanism.,Borges RJ, Cardoso FF, Fernandes CA, Dreyer TR, de Moraes DS, Floriano RS, Rodrigues-Simioni L, Fontes MR Biochim Biophys Acta. 2017 Jan;1861(1 Pt A):3199-3209. doi:, 10.1016/j.bbagen.2016.08.003. Epub 2016 Aug 13. PMID:27531710<ref>PMID:27531710</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Fontes, M.R.M]]
+<div class="pdbe-citations 4wtb" style="background-color:#fffaf0;"></div>
-[[Category: Borges, R.J]]
+== References ==

OCA at 19:57, 24 December 2014

2014-12-24T19:57:20Z

← Older revision		Revision as of 19:57, 24 December 2014
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	Authors: Borges, R.J., Fontes, M.R.M.		Authors: Borges, R.J., Fontes, M.R.M.

	Description:		Description:
			[[Category: Unreleased Structures]]
			[[Category: Fontes, M.R.M]]
			[[Category: Borges, R.J]]

OCA: Protected "4wtb" [edit=sysop:move=sysop]

2014-11-05T08:08:37Z

Protected "4wtb" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:08, 5 November 2014
(No difference)

OCA: New page: '''Unreleased structure''' The entry 4wtb is ON HOLD Authors: Borges, R.J., Fontes, M.R.M. Description:

2014-11-05T08:08:36Z

New page: '''Unreleased structure''' The entry 4wtb is ON HOLD Authors: Borges, R.J., Fontes, M.R.M. Description:

New page

'''Unreleased structure'''

The entry 4wtb is ON HOLD

Authors: Borges, R.J., Fontes, M.R.M.

Description: