5bys - Revision history

OCA at 18:22, 8 September 2026

2026-09-08T18:22:45Z

← Older revision		Revision as of 18:22, 8 September 2026
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BYS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BYS FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat">~~<scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>,~~ <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG~~</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4~~</scene></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [https://pdbe.org/5bys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [https://www.ebi.ac.uk/pdbsum/5bys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [https://pdbe.org/5bys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [https://www.ebi.ac.uk/pdbsum/5bys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:17, 10 January 2024

2024-01-10T11:17:35Z

← Older revision		Revision as of 11:17, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BYS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BYS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [https://pdbe.org/5bys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [https://www.ebi.ac.uk/pdbsum/5bys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [https://pdbe.org/5bys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [https://www.ebi.ac.uk/pdbsum/5bys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:12, 7 June 2023

2023-06-07T06:12:59Z

← Older revision		Revision as of 06:12, 7 June 2023
Line 3:		Line 3:
	<StructureSection load='5bys' size='340' side='right'caption='[[5bys]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='5bys' size='340' side='right'caption='[[5bys]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5BYS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BYS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xdd\|4xdd]], [[4xdc\|4xdc]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [https://pdbe.org/5bys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [https://www.ebi.ac.uk/pdbsum/5bys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [~~http~~://pdbe.org/5bys PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5bys PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PHF1_CLOPA PHF1_CLOPA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Ferredoxin hydrogenase~~]]		[[Category: Clostridium pasteurianum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Engelbrecht, V]]		[[Category: Engelbrecht V]]
	[[Category: Esselborn, J]]		[[Category: Esselborn J]]
	[[Category: Happe, T]]		[[Category: Happe T]]
	[[Category: Hofmann, E]]		[[Category: Hofmann E]]
	[[Category: Kurisu, G]]		[[Category: Kurisu G]]
	[[Category: Muraki, N]]		[[Category: Muraki N]]
	~~[[Category: H-cluster]]~~
	~~[[Category: Hydrogenase]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Semisynthetic enzyme~~]]

OCA at 08:02, 15 April 2020

2020-04-15T08:02:17Z

← Older revision		Revision as of 08:02, 15 April 2020
Line 1:		Line 1:

	==Semisynthetic [FeFe]-hydrogenase CpI with sulfur-dithiolato-bridged [2Fe] cofactor==		==Semisynthetic [FeFe]-hydrogenase CpI with sulfur-dithiolato-bridged [2Fe] cofactor==
	<StructureSection load='5bys' size='340' side='right' caption='[[5bys]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='5bys' size='340' side='right'caption='[[5bys]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5BYS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5BYS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xdd\|4xdd]], [[4xdc\|4xdc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xdd\|4xdd]], [[4xdc\|4xdc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [http://pdbe.org/5bys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [http://www.ebi.ac.uk/pdbsum/5bys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [http://pdbe.org/5bys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [http://www.ebi.ac.uk/pdbsum/5bys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Ferredoxin hydrogenase]]		[[Category: Ferredoxin hydrogenase]]
			[[Category: Large Structures]]
	[[Category: Engelbrecht, V]]		[[Category: Engelbrecht, V]]
	[[Category: Esselborn, J]]		[[Category: Esselborn, J]]

OCA at 06:01, 11 July 2018

2018-07-11T06:01:24Z

← Older revision		Revision as of 06:01, 11 July 2018
Line 3:		Line 3:
	<StructureSection load='5bys' size='340' side='right' caption='[[5bys]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='5bys' size='340' side='right' caption='[[5bys]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BYS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BYS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xdd\|4xdd]], [[4xdc\|4xdc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xdd\|4xdd]], [[4xdc\|4xdc]]</td></tr>
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [http://pdbe.org/5bys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [http://www.ebi.ac.uk/pdbsum/5bys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [http://pdbe.org/5bys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [http://www.ebi.ac.uk/pdbsum/5bys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			[FeFe]-hydrogenases are nature's fastest catalysts for the evolution or oxidation of hydrogen. Numerous synthetic model complexes for the [2Fe] subcluster (2FeH) of their active site are known, but so far none of these could compete with the enzymes. The complex Fe2[mu-(SCH2)2X](CN)2(CO)4(2-) with X = NH was shown to integrate into the apo-form of [FeFe]-hydrogenases to yield a fully active enzyme. Here we report the first crystal structures of the apo-form of the bacterial [FeFe]-hydrogenase CpI from Clostridium pasteurianum at 1.60 A and the active semisynthetic enzyme, CpI(ADT), at 1.63 A. The structures illustrate the significant changes in ligand coordination upon integration and activation of the [2Fe] complex. These changes are induced by a rigid 2FeH cavity as revealed by the structure of apoCpI, which is remarkably similar to CpI(ADT). Additionally we present the high resolution crystal structures of the semisynthetic bacterial [FeFe]-hydrogenases CpI(PDT) (X = CH2), CpI(ODT) (X = O) and CpI(SDT) (X = S) with changes in the headgroup of the dithiolate bridge in the 2FeH cofactor. The structures of these inactive enzymes demonstrate that the 2FeH-subcluster and its protein environment remain largely unchanged when compared to the active enzyme CpI(ADT). As the active site shows an open coordination site in all structures, the absence of catalytic activity is probably not caused by steric obstruction. This demonstrates that the chemical properties of the dithiolate bridge are essential for enzyme activity.

			A structural view of synthetic cofactor integration into [FeFe]-hydrogenases.,Esselborn J, Muraki N, Klein K, Engelbrecht V, Metzler-Nolte N, Apfel UP, Hofmann E, Kurisu G, Happe T Chem Sci. 2016 Feb 1;7(2):959-968. doi: 10.1039/c5sc03397g. Epub 2015 Oct 26. PMID:29896366<ref>PMID:29896366</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5bys" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:27, 12 October 2017

2017-10-12T14:27:32Z

← Older revision		Revision as of 14:27, 12 October 2017
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	~~'''Unreleased structure'''~~

	~~The entry~~ 5bys ~~is ON HOLD~~		==Semisynthetic [FeFe]-hydrogenase CpI with sulfur-dithiolato-bridged [2Fe] cofactor==
			<StructureSection load='5bys' size='340' side='right' caption='[[5bys]], [[Resolution\|resolution]] 1.93Å' scene=''>
	~~Authors~~: ~~Esselborn, J~~.~~, Muraki, N~~.~~, Engelbrecht, V~~.~~, Hofmann, E~~., ~~Kurisu~~, G., ~~Happe~~, T.		== Structural highlights ==
			<table><tr><td colspan='2'>[[5bys]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BYS FirstGlance]. <br>
	~~Description~~: ~~Semisynthetic~~ [~~FeFe~~]~~-hydrogenase CpI~~ with ~~sulfuro~~-~~dimethyl~~-~~dithiolato~~-~~bridged~~ [~~2Fe2S~~] ~~cofactor~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4WX:BIS(CYANIDO-KAPPAC)(DICARBONYL)-MU-(OXOMETHYLIDENE)-MU-(SULFANEDIYLDIMETHANETHIOLATATO-1KAPPAS 2KAPPAS)DIIRON(2+)'>4WX</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xdd\|4xdd]], [[4xdc\|4xdc]]</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin_hydrogenase Ferredoxin hydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.12.7.2 1.12.7.2] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bys OCA], [http://pdbe.org/5bys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bys RCSB], [http://www.ebi.ac.uk/pdbsum/5bys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bys ProSAT]</span></td></tr>
			</table>
			__TOC__
			</StructureSection>
			[[Category: Ferredoxin hydrogenase]]
	[[Category: Engelbrecht, V]]		[[Category: Engelbrecht, V]]
	~~[[Category: Muraki, N]]~~
	~~[[Category: Kurisu, G]]~~
	[[Category: Esselborn, J]]		[[Category: Esselborn, J]]
	[[Category: Happe, T]]		[[Category: Happe, T]]
	[[Category: Hofmann, E]]		[[Category: Hofmann, E]]
			[[Category: Kurisu, G]]
			[[Category: Muraki, N]]
			[[Category: H-cluster]]
			[[Category: Hydrogenase]]
			[[Category: Oxidoreductase]]
			[[Category: Semisynthetic enzyme]]

OCA: Protected "5bys" [edit=sysop:move=sysop]

2015-06-17T14:05:06Z

Protected "5bys" [edit=sysop:move=sysop]

← Older revision	Revision as of 14:05, 17 June 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5bys is ON HOLD Authors: Esselborn, J., Muraki, N., Engelbrecht, V., Hofmann, E., Kurisu, G., Happe, T. Description: Semisynthetic [FeFe]-hydrogena...

2015-06-17T14:05:05Z

New page: '''Unreleased structure''' The entry 5bys is ON HOLD Authors: Esselborn, J., Muraki, N., Engelbrecht, V., Hofmann, E., Kurisu, G., Happe, T. Description: Semisynthetic [FeFe]-hydrogena...

New page

'''Unreleased structure'''

The entry 5bys is ON HOLD

Authors: Esselborn, J., Muraki, N., Engelbrecht, V., Hofmann, E., Kurisu, G., Happe, T.

Description: Semisynthetic [FeFe]-hydrogenase CpI with sulfuro-dimethyl-dithiolato-bridged [2Fe2S] cofactor
[[Category: Unreleased Structures]]
[[Category: Engelbrecht, V]]
[[Category: Muraki, N]]
[[Category: Kurisu, G]]
[[Category: Esselborn, J]]
[[Category: Happe, T]]
[[Category: Hofmann, E]]