5cc3 - Revision history

OCA at 08:36, 27 September 2023

2023-09-27T08:36:43Z

← Older revision		Revision as of 08:36, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5cc3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CC3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5cc3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CC3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4ZU:6-BROMANYL-1H-INDOLE-2-CARBOXYLIC+ACID'>4ZU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.204Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4ZU:6-BROMANYL-1H-INDOLE-2-CARBOXYLIC+ACID'>4ZU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cc3 OCA], [https://pdbe.org/5cc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cc3 RCSB], [https://www.ebi.ac.uk/pdbsum/5cc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cc3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cc3 OCA], [https://pdbe.org/5cc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cc3 RCSB], [https://www.ebi.ac.uk/pdbsum/5cc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cc3 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:58, 14 June 2023

2023-06-14T11:58:05Z

← Older revision		Revision as of 11:58, 14 June 2023
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	==Crystal structure of Mycobacterium tuberculosis malate synthase in complex with 6-bromo-1H-indole-2-carboxylic acid==		==Crystal structure of Mycobacterium tuberculosis malate synthase in complex with 6-bromo-1H-indole-2-carboxylic acid==
	<StructureSection load='5cc3' size='340' side='right' caption='[[5cc3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='5cc3' size='340' side='right'caption='[[5cc3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5cc3]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CC3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5CC3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5cc3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CC3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4ZU:6-BROMANYL-1H-INDOLE-2-CARBOXYLIC+ACID'>4ZU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4ZU:6-BROMANYL-1H-INDOLE-2-CARBOXYLIC+ACID'>4ZU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glcB, Rv1837c, MTCY1A11.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cc3 OCA], [https://pdbe.org/5cc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cc3 RCSB], [https://www.ebi.ac.uk/pdbsum/5cc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cc3 ProSAT]</span></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cc3 OCA], [~~http~~://pdbe.org/5cc3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5cc3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5cc3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5cc3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/MASZ_MYCTU MASZ_MYCTU]] Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA (By similarity).[HAMAP-Rule:MF_00641]		[https://www.uniprot.org/uniprot/MASZ_MYCTU MASZ_MYCTU] Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA (By similarity).[HAMAP-Rule:MF_00641]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Establishment or maintenance of a persistent infection by Mycobacterium tuberculosis requires the glyoxylate pathway. This is a bypass of the tricarboxylic acid cycle in which isocitrate lyase and malate synthase (GlcB) catalyze the net incorporation of carbon during growth of microorganisms on acetate or fatty acids as the primary carbon source. The glcB gene from M. tuberculosis, which encodes malate synthase, was cloned, and GlcB was expressed in Escherichia coli. The influence of media conditions on expression in M. tuberculosis indicated that this enzyme is regulated differentially to isocitrate lyase. Purified GlcB had K(m) values of 57 and 30 microm for its substrates glyoxylate and acetyl coenzyme A, respectively, and was inhibited by bromopyruvate, oxalate, and phosphoenolpyruvate. The GlcB structure was solved to 2.1-A resolution in the presence of glyoxylate and magnesium. We also report the structure of GlcB in complex with the products of the reaction, coenzyme A and malate, solved to 2.7-A resolution. Coenzyme A binds in a bent conformation, and the details of its interactions are described, together with implications on the enzyme mechanism.

	Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis.,Smith CV, Huang CC, Miczak A, Russell DG, Sacchettini JC, Honer zu Bentrup K J Biol Chem. 2003 Jan 17;278(3):1735-43. Epub 2002 Oct 21. PMID:12393860<ref>PMID:12393860</ref>		==See Also==
			*[[Malate synthase 3D structures\|Malate synthase 3D structures]]
	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 5cc3" style~~=~~"background-color:#fffaf0;"></div>~~
	=~~= References~~ ==
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Malate synthase~~]]		[[Category: Large Structures]]
	[[Category: ~~Myctu~~]]		[[Category: Mycobacterium tuberculosis H37Rv]]
	[[Category: Huang, H L]]		[[Category: Huang H-L]]
	[[Category: Sacchettini~~, J C]]~~		[[Category: Sacchettini JC]]
	~~[[Category: Complex]]~~
	~~[[Category: Fragment]]~~
	~~[[Category: Transferase~~]]

OCA at 12:26, 6 November 2017

2017-11-06T12:26:26Z

← Older revision		Revision as of 12:26, 6 November 2017
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	<StructureSection load='5cc3' size='340' side='right' caption='[[5cc3]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='5cc3' size='340' side='right' caption='[[5cc3]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5cc3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CC3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CC3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5cc3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CC3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CC3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4ZU:6-BROMANYL-1H-INDOLE-2-CARBOXYLIC+ACID'>4ZU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4ZU:6-BROMANYL-1H-INDOLE-2-CARBOXYLIC+ACID'>4ZU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glcB, Rv1837c, MTCY1A11.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cc3 OCA], [http://pdbe.org/5cc3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cc3 RCSB], [http://www.ebi.ac.uk/pdbsum/5cc3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cc3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cc3 OCA], [http://pdbe.org/5cc3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cc3 RCSB], [http://www.ebi.ac.uk/pdbsum/5cc3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cc3 ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Malate synthase]]		[[Category: Malate synthase]]
			[[Category: Myctu]]
	[[Category: Huang, H L]]		[[Category: Huang, H L]]
	[[Category: Sacchettini, J C]]		[[Category: Sacchettini, J C]]

OCA at 16:02, 10 August 2016

2016-08-10T16:02:35Z

@@ Line 1: / Line 1: @@
-The entry 5cc3 is ON HOLD  until Paper Publication
+==Crystal structure of Mycobacterium tuberculosis malate synthase in complex with 6-bromo-1H-indole-2-carboxylic acid==
-Authors: Huang, H.-L., Sacchettini, J.C.
+Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis.,Smith CV, Huang CC, Miczak A, Russell DG, Sacchettini JC, Honer zu Bentrup K J Biol Chem. 2003 Jan 17;278(3):1735-43. Epub 2002 Oct 21. PMID:12393860<ref>PMID:12393860</ref>
-Description: Crystal structure of Mycobacterium tuberculosis malate synthase in complex with 6-bromo-1H-indole-2-carboxylic acid
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Sacchettini, J.C]]
+<div class="pdbe-citations 5cc3" style="background-color:#fffaf0;"></div>
-[[Category: Huang, H.-L]]
+== References ==

OCA at 13:00, 12 August 2015

2015-08-12T13:00:39Z

← Older revision		Revision as of 13:00, 12 August 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5cc3 is ON HOLD		The entry 5cc3 is ON HOLD until Paper Publication

	Authors: Huang, H.-L., Sacchettini, J.C.		Authors: Huang, H.-L., Sacchettini, J.C.

OCA: Protected "5cc3" [edit=sysop:move=sysop]

2015-07-08T13:58:53Z

Protected "5cc3" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:58, 8 July 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5cc3 is ON HOLD Authors: Huang, H.-L., Sacchettini, J.C. Description: Crystal structure of Mycobacterium tuberculosis malate synthase in complex wi...

2015-07-08T13:58:52Z

New page: '''Unreleased structure''' The entry 5cc3 is ON HOLD Authors: Huang, H.-L., Sacchettini, J.C. Description: Crystal structure of Mycobacterium tuberculosis malate synthase in complex wi...

New page

'''Unreleased structure'''

The entry 5cc3 is ON HOLD

Authors: Huang, H.-L., Sacchettini, J.C.

Description: Crystal structure of Mycobacterium tuberculosis malate synthase in complex with 6-bromo-1H-indole-2-carboxylic acid
[[Category: Unreleased Structures]]
[[Category: Sacchettini, J.C]]
[[Category: Huang, H.-L]]