5daq - Revision history

OCA at 11:25, 10 January 2024

2024-01-10T11:25:29Z

← Older revision		Revision as of 11:25, 10 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5daq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DAQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5daq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DAQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=58D:(3S)-3-(4-METHOXYBENZYL)-4-METHYL-3,4-DIHYDRO-1H-1,4-BENZODIAZEPINE-2,5-DIONE'>58D</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=58D:(3S)-3-(4-METHOXYBENZYL)-4-METHYL-3,4-DIHYDRO-1H-1,4-BENZODIAZEPINE-2,5-DIONE'>58D</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5daq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daq OCA], [https://pdbe.org/5daq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5daq RCSB], [https://www.ebi.ac.uk/pdbsum/5daq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5daq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5daq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daq OCA], [https://pdbe.org/5daq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5daq RCSB], [https://www.ebi.ac.uk/pdbsum/5daq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5daq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:28, 21 June 2023

2023-06-21T10:28:37Z

← Older revision		Revision as of 10:28, 21 June 2023
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	<StructureSection load='5daq' size='340' side='right'caption='[[5daq]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5daq' size='340' side='right'caption='[[5daq]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5daq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aspergillus_nidulans~~ Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5DAQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5daq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DAQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=58D:(3S)-3-(4-METHOXYBENZYL)-4-METHYL-3,4-DIHYDRO-1H-1,4-BENZODIAZEPINE-2,5-DIONE'>58D</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=58D:(3S)-3-(4-METHOXYBENZYL)-4-METHYL-3,4-DIHYDRO-1H-1,4-BENZODIAZEPINE-2,5-DIONE'>58D</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dap\|5dap]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5daq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daq OCA], [https://pdbe.org/5daq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5daq RCSB], [https://www.ebi.ac.uk/pdbsum/5daq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5daq ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AN9227.2, ANIA_09227 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5daq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daq OCA], [~~http~~://pdbe.org/5daq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5daq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5daq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5daq ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/ASQJ_EMENI ASQJ_EMENI] Iron/alpha-ketoglutarate-dependent dioxygenase; part of the gene cluster that mediates the biosynthesis of the aspoquinolone mycotoxins (PubMed:25251934, PubMed:26553478). The first stage is catalyzed by the nonribosomal pepdide synthetase asqK that condenses anthranilic acid and O-methyl-L-tyrosine to produce 4'-methoxycyclopeptin (PubMed:25251934). AsqK is also able to use anthranilic acid and L-phenylalanine as substrates to produce cyclopeptin, but at a tenfold lower rate (PubMed:25251934). 4'-methoxycyclopeptin is then converted to 4'-methoxydehydrocyclopeptin by the ketoglutarate-dependent dioxygenase asqJ through dehydrogenation to form a double bond between C-alpha and C-beta of the O-methyltyrosine side chain (PubMed:25251934, PubMed:26553478). AsqJ also converts its first product 4'-methoxydehydrocyclopeptin to 4'-methoxycyclopenin (PubMed:25251934). AsqJ is a very unique dioxygenase which is capable of catalyzing radical-mediated dehydrogenation and epoxidation reactions sequentially on a 6,7-benzo-diazepinedione substrate in the 4'-methoxyviridicatin biosynthetic pathway (PubMed:25251934). The following conversion of 4'-methoxycyclopenin into 4'-methoxyviridicatin proceeds non-enzymatically (PubMed:25251934). AsqJ is also capable of converting cyclopeptin into dehydrocyclopeptin and cyclopenin in a sequential fashion (PubMed:25251934). Cyclopenin can be converted into viridicatin non-enzymatically (PubMed:25251934). 4'-methoxyviridicatin likely acts as a precursor of quinolone natural products, such as aspoquinolones, peniprequinolones, penigequinolones, and yaequinolones (PubMed:25251934). Further characterization of the remaining genes in the cluster has still to be done to determine the exact identity of quinolone products this cluster is responsible for biosynthesizing (PubMed:25251934).<ref>PMID:25251934</ref> <ref>PMID:26553478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5daq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5daq" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Dioxygenase 3D structures\|Dioxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Aspergillus nidulans]]		[[Category: Aspergillus nidulans FGSC A4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Braeuer, A]]		[[Category: Braeuer A]]
	[[Category: Groll, M]]		[[Category: Groll M]]
	~~[[Category: 4-methoxycyclopeptine]]~~
	~~[[Category: Alkaloid]]~~
	~~[[Category: Antibiotic]]~~
	~~[[Category: Biosynthesis]]~~
	~~[[Category: Desaturase]]~~
	~~[[Category: Epoxidase]]~~
	~~[[Category: Fragmentation]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Viridicatin~~]]

OCA at 07:57, 8 April 2020

2020-04-08T07:57:40Z

← Older revision		Revision as of 07:57, 8 April 2020
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	==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclopeptin==		==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclopeptin==
	<StructureSection load='5daq' size='340' side='right' caption='[[5daq]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5daq' size='340' side='right'caption='[[5daq]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5daq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=5DAQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5daq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DAQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=58D:(3S)-3-(4-METHOXYBENZYL)-4-METHYL-3,4-DIHYDRO-1H-1,4-BENZODIAZEPINE-2,5-DIONE'>58D</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=58D:(3S)-3-(4-METHOXYBENZYL)-4-METHYL-3,4-DIHYDRO-1H-1,4-BENZODIAZEPINE-2,5-DIONE'>58D</scene>, <scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dap\|5dap]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5dap\|5dap]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5daq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daq OCA], [http://pdbe.org/5daq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5daq RCSB], [http://www.ebi.ac.uk/pdbsum/5daq PDBsum]</span></td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AN9227.2, ANIA_09227 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5daq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daq OCA], [http://pdbe.org/5daq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5daq RCSB], [http://www.ebi.ac.uk/pdbsum/5daq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5daq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Aspergillus nidulans]]
			[[Category: Large Structures]]
	[[Category: Braeuer, A]]		[[Category: Braeuer, A]]
	[[Category: Groll, M]]		[[Category: Groll, M]]

OCA at 04:31, 1 December 2015

2015-12-01T04:31:42Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclopeptin==
-The entry 5daq is ON HOLD  until Paper Publication
+Structure of the Dioxygenase AsqJ: Mechanistic Insights into a One-Pot Multistep Quinolone Antibiotic Biosynthesis.,Brauer A, Beck P, Hintermann L, Groll M Angew Chem Int Ed Engl. 2015 Nov 10. doi: 10.1002/anie.201507835. PMID:26553478<ref>PMID:26553478</ref>
-Authors: Groll, M., Braeuer, A.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclopeptin
+<div class="pdbe-citations 5daq" style="background-color:#fffaf0;"></div>
-[[Category: Unreleased Structures]]
+== References ==
 [[Category: Groll, M]]
-[[Category: Braeuer, A]]
+[[Category: 4-methoxycyclopeptine]]

OCA at 11:23, 2 September 2015

2015-09-02T11:23:04Z

← Older revision		Revision as of 11:23, 2 September 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5daq is ON HOLD		The entry 5daq is ON HOLD until Paper Publication

	Authors: Groll, M., Braeuer, A.		Authors: Groll, M., Braeuer, A.

	Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-~~Methoxycyclopeptine~~		Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclopeptin
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Groll, M]]		[[Category: Groll, M]]
	[[Category: Braeuer, A]]		[[Category: Braeuer, A]]

OCA: Protected "5daq" [edit=sysop:move=sysop]

2015-08-26T12:40:33Z

Protected "5daq" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:40, 26 August 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5daq is ON HOLD Authors: Groll, M., Braeuer, A. Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclop...

2015-08-26T12:40:32Z

New page: '''Unreleased structure''' The entry 5daq is ON HOLD Authors: Groll, M., Braeuer, A. Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclop...

New page

'''Unreleased structure'''

The entry 5daq is ON HOLD

Authors: Groll, M., Braeuer, A.

Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase AsqJ in complex with 4-Methoxycyclopeptine
[[Category: Unreleased Structures]]
[[Category: Groll, M]]
[[Category: Braeuer, A]]