5djs - Revision history

OCA at 21:44, 28 June 2023

2023-06-28T21:44:25Z

← Older revision		Revision as of 21:44, 28 June 2023
Line 3:		Line 3:
	<StructureSection load='5djs' size='340' side='right'caption='[[5djs]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5djs' size='340' side='right'caption='[[5djs]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5djs]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"thermobaculum_terrenum"_botero_et_al._2004 "thermobaculum~~ terrenum~~" botero et al. 2004~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5DJS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5djs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobaculum_terrenum Thermobaculum terrenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DJS FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=UDP:URIDINE~~-~~5-DIPHOSPHATE'>UDP</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Tter_2822 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=166501 "Thermobaculum terrenum" Botero et al. 2004])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5djs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djs OCA], [~~http~~://pdbe.org/5djs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5djs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5djs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5djs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5djs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djs OCA], [https://pdbe.org/5djs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5djs RCSB], [https://www.ebi.ac.uk/pdbsum/5djs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5djs ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/D1CIY5_THET1 D1CIY5_THET1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Thermobaculum terrenum botero et al. 2004]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Aalten, D M.F van~~]]		[[Category: Thermobaculum terrenum]]
	[[Category: Ferenbach~~, A T~~]]		[[Category: Ferenbach AT]]
	[[Category: Gundogdu, M]]		[[Category: Gundogdu M]]
	[[Category: Lebedev, A]]		[[Category: Lebedev A]]
	[[Category: Ostrowski, A]]		[[Category: Ostrowski A]]
	[[Category: ~~Gt41]]~~		[[Category: Van Aalten DMF]]
	~~[[Category: O-glcnac transferase]]~~
	~~[[Category: Ogt]]~~
	~~[[Category: Transferase]]~~
	~~[[Category: Udp~~]]

OCA at 08:06, 8 April 2020

2020-04-08T08:06:47Z

← Older revision		Revision as of 08:06, 8 April 2020
Line 1:		Line 1:

	==Thermobaculum terrenum O-GlcNAc transferase mutant - K341M==		==Thermobaculum terrenum O-GlcNAc transferase mutant - K341M==
	<StructureSection load='5djs' size='340' side='right' caption='[[5djs]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5djs' size='340' side='right'caption='[[5djs]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5djs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=5DJS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5djs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermobaculum_terrenum"_botero_et_al._2004 "thermobaculum terrenum" botero et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DJS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5djs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djs OCA], [http://pdbe.org/5djs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djs RCSB], [http://www.ebi.ac.uk/pdbsum/5djs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5djs ProSAT]</span></td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tter_2822 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=166501 "Thermobaculum terrenum" Botero et al. 2004])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5djs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djs OCA], [http://pdbe.org/5djs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djs RCSB], [http://www.ebi.ac.uk/pdbsum/5djs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5djs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:

	==See Also==		==See Also==
	*[[O-GlcNAc transferase\|O-GlcNAc transferase]]		*[[O-GlcNAc transferase 3D structures\|O-GlcNAc transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Thermobaculum terrenum botero et al. 2004]]
			[[Category: Large Structures]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]
	[[Category: Ferenbach, A T]]		[[Category: Ferenbach, A T]]

OCA at 17:10, 18 August 2017

2017-08-18T17:10:28Z

← Older revision		Revision as of 17:10, 18 August 2017
Line 1:		Line 1:

	==Thermobaculum terrenum O-GlcNAc transferase mutant - K341M==		==Thermobaculum terrenum O-GlcNAc transferase mutant - K341M==
	<StructureSection load='5djs' size='340' side='right' caption='[[5djs]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5djs' size='340' side='right' caption='[[5djs]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[5djs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DJS FirstGlance]. <br>		<table><tr><td colspan='2'>[[5djs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DJS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5djs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djs OCA], [http://pdbe.org/5djs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djs RCSB], [http://www.ebi.ac.uk/pdbsum/5djs PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5djs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djs OCA], [http://pdbe.org/5djs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djs RCSB], [http://www.ebi.ac.uk/pdbsum/5djs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5djs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 5djs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5djs" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[O-GlcNAc transferase\|O-GlcNAc transferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:11, 30 November 2015

2015-11-30T19:11:22Z

← Older revision		Revision as of 19:11, 30 November 2015
Line 1:		Line 1:
	'''~~Unreleased~~ structure'''		==Thermobaculum terrenum O-GlcNAc transferase mutant - K341M==
			<StructureSection load='5djs' size='340' side='right' caption='[[5djs]], [[Resolution\|resolution]] 2.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5djs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DJS FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5djs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djs OCA], [http://pdbe.org/5djs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djs RCSB], [http://www.ebi.ac.uk/pdbsum/5djs PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Post-translational modification of proteins is a ubiquitous mechanism of signal transduction in all kingdoms of life. One such modification is addition of O-linked N-acetylglucosamine to serine or threonine residues, known as O-GlcNAcylation. This unusual type of glycosylation is thought to be restricted to nucleocytoplasmic proteins of eukaryotes and is mediated by a pair of O-GlcNAc transferase and O-GlcNAc hydrolase enzymes operating on a large number of substrate proteins. Protein O-GlcNAcylation is responsive to glucose and flux through the hexosamine biosynthetic pathway. Thus, a close relationship is thought to exist between the level of O-GlcNAc proteins within and the general metabolic state of the cell. While isolated apparent orthologues of these enzymes are present in bacterial genomes, their biological functions remain largely unexplored. It is possible that understanding the function of these proteins will allow development of reductionist models to uncover the principles of O-GlcNAc signalling. Here, we identify orthologues of both O-GlcNAc cycling enzymes in the genome of the thermophilic eubacterium Thermobaculum terrenum. The O-GlcNAcase and O-GlcNAc transferase are co-expressed and, like their mammalian orthologues, localise to the cytoplasm. The O-GlcNAcase orthologue possesses activity against O-GlcNAc proteins and model substrates. We describe crystal structures of both enzymes, including an O-GlcNAcase-peptide complex, showing conservation of active sites with the human orthologues. Although in vitro activity of the O-GlcNAc transferase could not be detected, treatment of T. terrenum with an O-GlcNAc transferase inhibitor led to inhibition of growth. T. terrenum may be the first example of a bacterium possessing a functional O-GlcNAc system.

	~~The entry 5djs is ON HOLD~~		Evidence for a functional O-GlcNAc system in the thermophilic bacterium Thermobaculum terrenum.,Ostrowski A, Gundogdu M, Ferenbach AT, Lebedev AA, van Aalten DM J Biol Chem. 2015 Oct 21. pii: jbc.M115.689596. PMID:26491011<ref>PMID:26491011</ref>

	~~Authors: Ostrowski~~, A.~~, Gundogdu, M., Ferenbach, A.T., Lebedev, A., van Aalten, D.M~~.F.		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~Description: Thermobaculum terrenum O~~-~~GlcNAc transferase mutant~~ -~~K341M~~		<div class="pdbe-citations 5djs" style="background-color:#fffaf0;"></div>
	[[Category: ~~Unreleased Structures~~]]		== References ==
	[[Category: Ferenbach, A.T]]		<references/>
			__TOC__
			</StructureSection>
			[[Category: Aalten, D M.F van]]
			[[Category: Ferenbach, A T]]
			[[Category: Gundogdu, M]]
	[[Category: Lebedev, A]]		[[Category: Lebedev, A]]
	~~[[Category: Van Aalten, D.M.F]]~~
	[[Category: Ostrowski, A]]		[[Category: Ostrowski, A]]
	[[Category: ~~Gundogdu, M~~]]		[[Category: Gt41]]
			[[Category: O-glcnac transferase]]
			[[Category: Ogt]]
			[[Category: Transferase]]
			[[Category: Udp]]

OCA: Protected "5djs" [edit=sysop:move=sysop]

2015-09-09T10:19:06Z

Protected "5djs" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:19, 9 September 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5djs is ON HOLD Authors: Ostrowski, A., Gundogdu, M., Ferenbach, A.T., Lebedev, A., van Aalten, D.M.F. Description: Thermobaculum terrenum O-GlcNAc...

2015-09-09T10:19:04Z

New page: '''Unreleased structure''' The entry 5djs is ON HOLD Authors: Ostrowski, A., Gundogdu, M., Ferenbach, A.T., Lebedev, A., van Aalten, D.M.F. Description: Thermobaculum terrenum O-GlcNAc...

New page

'''Unreleased structure'''

The entry 5djs is ON HOLD

Authors: Ostrowski, A., Gundogdu, M., Ferenbach, A.T., Lebedev, A., van Aalten, D.M.F.

Description: Thermobaculum terrenum O-GlcNAc transferase mutant -K341M
[[Category: Unreleased Structures]]
[[Category: Ferenbach, A.T]]
[[Category: Lebedev, A]]
[[Category: Van Aalten, D.M.F]]
[[Category: Ostrowski, A]]
[[Category: Gundogdu, M]]