5erc - Revision history

OCA at 12:29, 6 March 2024

2024-03-06T12:29:45Z

← Older revision		Revision as of 12:29, 6 March 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/BRPF1_HUMAN BRPF1_HUMAN] Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2.<ref>PMID:16387653</ref> <ref>PMID:18794358</ref>		[https://www.uniprot.org/uniprot/BRPF1_HUMAN BRPF1_HUMAN] Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2.<ref>PMID:16387653</ref> <ref>PMID:18794358</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	BRPF1 (bromodomain PHD finger 1) is a core subunit of the MOZ histone acetyltransferase (HAT) complex, critical for normal developmental programs and implicated in acute leukemias. BRPF1 contains a unique assembly of zinc fingers, termed a PZP domain, the physiological role of which remains unclear. Here, we elucidate the structure-function relationship of this novel epigenetic reader and detail the biological and mechanistic consequences of its interaction with nucleosomes. PZP has a globular architecture and forms a 2:1 stoichiometry complex with the nucleosome, bivalently interacting with histone H3 and DNA. This binding impacts the nucleosome dynamics, shifting the DNA unwrapping/rewrapping equilibrium toward the unwrapped state and increasing DNA accessibility. We demonstrate that the DNA-binding function of the BRPF1 PZP domain is required for the MOZ-BRPF1-ING5-hEaf6 HAT complex to be recruited to chromatin and to acetylate nucleosomal histones. Our findings reveal a novel link between chromatin dynamics and MOZ-mediated acetylation.

	Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation.,Klein BJ, Muthurajan UM, Lalonde ME, Gibson MD, Andrews FH, Hepler M, Machida S, Yan K, Kurumizaka H, Poirier MG, Cote J, Luger K, Kutateladze TG Nucleic Acids Res. 2015 Nov 30. pii: gkv1321. PMID:26626149<ref>PMID:26626149</ref>		==See Also==
			*[[Peregrin\|Peregrin]]
	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class~~=~~"pdbe-citations 5erc" style~~=~~"background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:18, 12 July 2023

2023-07-12T08:18:38Z

← Older revision		Revision as of 08:18, 12 July 2023
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	<StructureSection load='5erc' size='340' side='right'caption='[[5erc]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='5erc' size='340' side='right'caption='[[5erc]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5erc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ERC FirstGlance]. <br>		<table><tr><td colspan='2'>[[5erc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ERC FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CA:CALCIUM+ION'>CA</scene~~>, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~BRPF1, BR140 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5erc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5erc OCA], [~~http~~://pdbe.org/5erc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5erc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5erc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5erc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5erc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5erc OCA], [https://pdbe.org/5erc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5erc RCSB], [https://www.ebi.ac.uk/pdbsum/5erc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5erc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/BRPF1_HUMAN BRPF1_HUMAN]] Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2.<ref>PMID:16387653</ref> <ref>PMID:18794358</ref>		[https://www.uniprot.org/uniprot/BRPF1_HUMAN BRPF1_HUMAN] Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2.<ref>PMID:16387653</ref> <ref>PMID:18794358</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5erc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5erc" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Peregrin\|Peregrin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Andrews~~, F H~~]]		[[Category: Andrews FH]]
	[[Category: Klein~~, B J~~]]		[[Category: Klein BJ]]
	[[Category: Kutateladze~~, T G]]~~		[[Category: Kutateladze TG]]
	~~[[Category: Dna]]~~
	~~[[Category: H3 histone]]~~
	~~[[Category: Methyllysine]]~~
	~~[[Category: Reader domain]]~~
	~~[[Category: Transcription~~]]

OCA at 09:02, 4 December 2019

2019-12-04T09:02:20Z

← Older revision		Revision as of 09:02, 4 December 2019
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	==X-ray crystal structure of BRPF1 PZP domain==		==X-ray crystal structure of BRPF1 PZP domain==
	<StructureSection load='5erc' size='340' side='right' caption='[[5erc]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='5erc' size='340' side='right'caption='[[5erc]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5erc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ERC FirstGlance]. <br>		<table><tr><td colspan='2'>[[5erc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ERC FirstGlance]. <br>
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	==See Also==		==See Also==
	*[[Peregrin ~~(BRPF1)~~\|Peregrin ~~(BRPF1)~~]]		*[[Peregrin\|Peregrin]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Andrews, F H]]		[[Category: Andrews, F H]]
	[[Category: Klein, B J]]		[[Category: Klein, B J]]

OCA at 12:39, 6 November 2017

2017-11-06T12:39:49Z

← Older revision		Revision as of 12:39, 6 November 2017
Line 1:		Line 1:

	==X-ray crystal structure of BRPF1 PZP domain==		==X-ray crystal structure of BRPF1 PZP domain==
	<StructureSection load='5erc' size='340' side='right' caption='[[5erc]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='5erc' size='340' side='right' caption='[[5erc]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5erc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ERC FirstGlance]. <br>		<table><tr><td colspan='2'>[[5erc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ERC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5erc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5erc OCA], [http://pdbe.org/5erc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5erc RCSB], [http://www.ebi.ac.uk/pdbsum/5erc PDBsum]</span></td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BRPF1, BR140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5erc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5erc OCA], [http://pdbe.org/5erc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5erc RCSB], [http://www.ebi.ac.uk/pdbsum/5erc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5erc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5erc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5erc" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Peregrin (BRPF1)\|Peregrin (BRPF1)]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Human]]
	[[Category: Andrews, F H]]		[[Category: Andrews, F H]]
	[[Category: Klein, B J]]		[[Category: Klein, B J]]

OCA at 19:31, 30 December 2015

2015-12-30T19:31:35Z

← Older revision		Revision as of 19:31, 30 December 2015
Line 1:		Line 1:
	'''~~Unreleased~~ structure'''		==X-ray crystal structure of BRPF1 PZP domain==
			<StructureSection load='5erc' size='340' side='right' caption='[[5erc]], [[Resolution\|resolution]] 2.05Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5erc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ERC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ERC FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5erc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5erc OCA], [http://pdbe.org/5erc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5erc RCSB], [http://www.ebi.ac.uk/pdbsum/5erc PDBsum]</span></td></tr>
			</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/BRPF1_HUMAN BRPF1_HUMAN]] Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2.<ref>PMID:16387653</ref> <ref>PMID:18794358</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BRPF1 (bromodomain PHD finger 1) is a core subunit of the MOZ histone acetyltransferase (HAT) complex, critical for normal developmental programs and implicated in acute leukemias. BRPF1 contains a unique assembly of zinc fingers, termed a PZP domain, the physiological role of which remains unclear. Here, we elucidate the structure-function relationship of this novel epigenetic reader and detail the biological and mechanistic consequences of its interaction with nucleosomes. PZP has a globular architecture and forms a 2:1 stoichiometry complex with the nucleosome, bivalently interacting with histone H3 and DNA. This binding impacts the nucleosome dynamics, shifting the DNA unwrapping/rewrapping equilibrium toward the unwrapped state and increasing DNA accessibility. We demonstrate that the DNA-binding function of the BRPF1 PZP domain is required for the MOZ-BRPF1-ING5-hEaf6 HAT complex to be recruited to chromatin and to acetylate nucleosomal histones. Our findings reveal a novel link between chromatin dynamics and MOZ-mediated acetylation.

	~~The entry 5erc is ON HOLD~~		Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation.,Klein BJ, Muthurajan UM, Lalonde ME, Gibson MD, Andrews FH, Hepler M, Machida S, Yan K, Kurumizaka H, Poirier MG, Cote J, Luger K, Kutateladze TG Nucleic Acids Res. 2015 Nov 30. pii: gkv1321. PMID:26626149<ref>PMID:26626149</ref>

	~~Authors: Klein~~, B.J.~~, Andrews, F.H., Kutateladze, T.G~~.		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~Description~~: ~~X-ray crystal structure of BRPF1 PZP domain~~		<div class="pdbe-citations 5erc" style="background-color:#fffaf0;"></div>
	[[Category: ~~Unreleased Structures~~]]		== References ==
	[[Category: Klein, B.J]]		<references/>
	[[Category: Kutateladze, T.G]]		__TOC__
	[[Category: ~~Andrews, F.H~~]]		</StructureSection>
			[[Category: Andrews, F H]]
			[[Category: Klein, B J]]
			[[Category: Kutateladze, T G]]
			[[Category: Dna]]
			[[Category: H3 histone]]
			[[Category: Methyllysine]]
			[[Category: Reader domain]]
			[[Category: Transcription]]

OCA: Protected "5erc" [edit=sysop:move=sysop]

2015-12-01T03:15:59Z

Protected "5erc" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:15, 1 December 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5erc is ON HOLD Authors: Klein, B.J., Andrews, F.H., Kutateladze, T.G. Description: X-ray crystal structure of BRPF1 PZP domain [[Category: Unrelea...

2015-12-01T03:15:58Z

New page: '''Unreleased structure''' The entry 5erc is ON HOLD Authors: Klein, B.J., Andrews, F.H., Kutateladze, T.G. Description: X-ray crystal structure of BRPF1 PZP domain [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 5erc is ON HOLD

Authors: Klein, B.J., Andrews, F.H., Kutateladze, T.G.

Description: X-ray crystal structure of BRPF1 PZP domain
[[Category: Unreleased Structures]]
[[Category: Klein, B.J]]
[[Category: Kutateladze, T.G]]
[[Category: Andrews, F.H]]