5ev2 - Revision history

OCA at 12:29, 6 March 2024

2024-03-06T12:29:57Z

← Older revision		Revision as of 12:29, 6 March 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>		[https://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	How the essential pre-mRNA splicing factor U2AF(65) recognizes the polypyrimidine (Py) signals of the major class of 3' splice sites in human gene transcripts remains incompletely understood. We determined four structures of an extended U2AF(65)-RNA-binding domain bound to Py-tract oligonucleotides at resolutions between 2.0 and 1.5 A. These structures together with RNA binding and splicing assays reveal unforeseen roles for U2AF(65) inter-domain residues in recognizing a contiguous, nine-nucleotide Py tract. The U2AF(65) linker residues between the dual RNA recognition motifs (RRMs) recognize the central nucleotide, whereas the N- and C-terminal RRM extensions recognize the 3' terminus and third nucleotide. Single-molecule FRET experiments suggest that conformational selection and induced fit of the U2AF(65) RRMs are complementary mechanisms for Py-tract association. Altogether, these results advance the mechanistic understanding of molecular recognition for a major class of splice site signals.

	An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.,Agrawal AA, Salsi E, Chatrikhi R, Henderson S, Jenkins JL, Green MR, Ermolenko DN, Kielkopf CL Nat Commun. 2016 Mar 8;7:10950. doi: 10.1038/ncomms10950. PMID:26952537<ref>PMID:26952537</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 5ev2" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:26, 12 July 2023

2023-07-12T08:26:54Z

← Older revision		Revision as of 08:26, 12 July 2023
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	<StructureSection load='5ev2' size='340' side='right'caption='[[5ev2]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='5ev2' size='340' side='right'caption='[[5ev2]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ev2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EV2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EV2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ev2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EV2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EV2 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=DIO:1,4~~-~~DIETHYLENE+DIOXIDE'>DIO</scene>~~, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.86Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DU:2-~~DEOXYURIDINE-5-MONOPHOSPHATE~~'>DU</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[5ev1\|5ev1]], [[5ev3\|5ev3]]~~, ~~[[5ev4\|5ev4]]~~<~~/td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ev2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ev2 OCA], [https://pdbe.org/5ev2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ev2 RCSB], [https://www.ebi.ac.uk/pdbsum/5ev2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ev2 ProSAT]</span></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">U2AF2, U2AF65 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ev2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ev2 OCA], [~~http~~://pdbe.org/5ev2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ev2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ev2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ev2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN]] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>		[https://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Agrawal, A A~~]]		[[Category: Synthetic construct]]
	[[Category: ~~Jenkins, J L~~]]		[[Category: Agrawal AA]]
	[[Category: ~~Kielkopf, C L~~]]		[[Category: Jenkins JL]]
	[[Category: ~~Polypyrimidine tract]]~~		[[Category: Kielkopf CL]]
	~~[[Category: Protein-rna complex]]~~
	~~[[Category: Rna binding protein]]~~
	~~[[Category: Rna binding protein-rna complex]]~~
	~~[[Category: Rna recognition motif]]~~
	~~[[Category: Rna splicing factor~~]]

OCA at 11:35, 25 December 2019

2019-12-25T11:35:56Z

← Older revision		Revision as of 11:35, 25 December 2019
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	==Structure II of Intact U2AF65 Recognizing the 3' Splice Site Signal==		==Structure II of Intact U2AF65 Recognizing the 3' Splice Site Signal==
	<StructureSection load='5ev2' size='340' side='right' caption='[[5ev2]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='5ev2' size='340' side='right'caption='[[5ev2]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ev2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EV2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ev2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EV2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ev1\|5ev1]], [[5ev3\|5ev3]], [[5ev4\|5ev4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ev1\|5ev1]], [[5ev3\|5ev3]], [[5ev4\|5ev4]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U2AF2, U2AF65 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ev2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ev2 OCA], [http://pdbe.org/5ev2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ev2 RCSB], [http://www.ebi.ac.uk/pdbsum/5ev2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ev2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ev2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ev2 OCA], [http://pdbe.org/5ev2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ev2 RCSB], [http://www.ebi.ac.uk/pdbsum/5ev2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ev2 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Agrawal, A A]]		[[Category: Agrawal, A A]]
	[[Category: Jenkins, J L]]		[[Category: Jenkins, J L]]

OCA at 10:12, 27 September 2017

2017-09-27T10:12:34Z

← Older revision		Revision as of 10:12, 27 September 2017
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ev1\|5ev1]], [[5ev3\|5ev3]], [[5ev4\|5ev4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ev1\|5ev1]], [[5ev3\|5ev3]], [[5ev4\|5ev4]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ev2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ev2 OCA], [http://pdbe.org/5ev2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ev2 RCSB], [http://www.ebi.ac.uk/pdbsum/5ev2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ev2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ev2 OCA], [http://pdbe.org/5ev2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ev2 RCSB], [http://www.ebi.ac.uk/pdbsum/5ev2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ev2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 18:37, 10 May 2016

2016-05-10T18:37:14Z

← Older revision		Revision as of 18:37, 10 May 2016
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN]] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>		[[http://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN]] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			How the essential pre-mRNA splicing factor U2AF(65) recognizes the polypyrimidine (Py) signals of the major class of 3' splice sites in human gene transcripts remains incompletely understood. We determined four structures of an extended U2AF(65)-RNA-binding domain bound to Py-tract oligonucleotides at resolutions between 2.0 and 1.5 A. These structures together with RNA binding and splicing assays reveal unforeseen roles for U2AF(65) inter-domain residues in recognizing a contiguous, nine-nucleotide Py tract. The U2AF(65) linker residues between the dual RNA recognition motifs (RRMs) recognize the central nucleotide, whereas the N- and C-terminal RRM extensions recognize the 3' terminus and third nucleotide. Single-molecule FRET experiments suggest that conformational selection and induced fit of the U2AF(65) RRMs are complementary mechanisms for Py-tract association. Altogether, these results advance the mechanistic understanding of molecular recognition for a major class of splice site signals.

			An extended U2AF(65)-RNA-binding domain recognizes the 3' splice site signal.,Agrawal AA, Salsi E, Chatrikhi R, Henderson S, Jenkins JL, Green MR, Ermolenko DN, Kielkopf CL Nat Commun. 2016 Mar 8;7:10950. doi: 10.1038/ncomms10950. PMID:26952537<ref>PMID:26952537</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5ev2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:20, 26 February 2016

2016-02-26T18:20:20Z

← Older revision		Revision as of 18:20, 26 February 2016
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	~~'''Unreleased structure'''~~

	~~The entry~~ 5ev2 is ~~ON HOLD until Paper Publication~~		==Structure II of Intact U2AF65 Recognizing the 3' Splice Site Signal==
			<StructureSection load='5ev2' size='340' side='right' caption='[[5ev2]], [[Resolution\|resolution]] 1.86Å' scene=''>
	~~Authors~~: ~~Agrawal~~, A.A., ~~Jenkins~~, J.L., ~~Kielkopf~~, C.L.		== Structural highlights ==
			<table><tr><td colspan='2'>[[5ev2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EV2 FirstGlance]. <br>
	~~Description:~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>
	[[Category: Jenkins, J.L]]		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ev1\|5ev1]], [[5ev3\|5ev3]], [[5ev4\|5ev4]]</td></tr>
	[[Category: Kielkopf, C.L]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ev2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ev2 OCA], [http://pdbe.org/5ev2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ev2 RCSB], [http://www.ebi.ac.uk/pdbsum/5ev2 PDBsum]</span></td></tr>
	[[Category: ~~Agrawal, A.A~~]]		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN]] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Agrawal, A A]]
			[[Category: Jenkins, J L]]
			[[Category: Kielkopf, C L]]
			[[Category: Polypyrimidine tract]]
			[[Category: Protein-rna complex]]
			[[Category: Rna binding protein]]
			[[Category: Rna binding protein-rna complex]]
			[[Category: Rna recognition motif]]
			[[Category: Rna splicing factor]]

OCA at 13:54, 10 February 2016

2016-02-10T13:54:52Z

← Older revision		Revision as of 13:54, 10 February 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5ev2 is ON HOLD		The entry 5ev2 is ON HOLD until Paper Publication

	Authors: Agrawal, A.A., Jenkins, J.L., Kielkopf, C.L.		Authors: Agrawal, A.A., Jenkins, J.L., Kielkopf, C.L.

OCA at 23:22, 27 January 2016

2016-01-27T23:22:42Z

← Older revision		Revision as of 23:22, 27 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5ev2 is ON HOLD ~~until Paper Publication~~		The entry 5ev2 is ON HOLD

	Authors: Agrawal, A.A., Jenkins, J.L., Kielkopf, C.L.		Authors: Agrawal, A.A., Jenkins, J.L., Kielkopf, C.L.

OCA at 10:56, 23 December 2015

2015-12-23T10:56:07Z

← Older revision		Revision as of 10:56, 23 December 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5ev2 is ON HOLD		The entry 5ev2 is ON HOLD until Paper Publication

	Authors: Agrawal, A.A., Jenkins, J.L., Kielkopf, C.L.		Authors: Agrawal, A.A., Jenkins, J.L., Kielkopf, C.L.

OCA: Protected "5ev2" [edit=sysop:move=sysop]

2015-12-01T03:25:38Z

Protected "5ev2" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:25, 1 December 2015
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