5evf - Revision history

OCA at 08:27, 12 July 2023

2023-07-12T08:27:19Z

← Older revision		Revision as of 08:27, 12 July 2023
Line 3:		Line 3:
	<StructureSection load='5evf' size='340' side='right'caption='[[5evf]], [[Resolution\|resolution]] 1.76Å' scene=''>		<StructureSection load='5evf' size='340' side='right'caption='[[5evf]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5evf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"francisella_tularensis_subsp~~._novicida~~"_(larson_et_al._1955)_sjostedt_2005 "francisella~~ tularensis subsp. novicida~~" (larson et al. 1955) sjostedt 2005~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5EVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[5evf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida Francisella tularensis subsp. novicida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EVF FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CL:CHLORIDE+ION'>CL</scene~~>, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.762Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class="sblockDat">[[5evg\|5evg]]</td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5evf OCA], [https://pdbe.org/5evf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5evf RCSB], [https://www.ebi.ac.uk/pdbsum/5evf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5evf ProSAT]</span></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">ACX55_1794 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5evf OCA], [~~http~~://pdbe.org/5evf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5evf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5evf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5evf ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0Q625_FRATN A0Q625_FRATN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Francisella tularensis subsp. novicida]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Craig, L]]		[[Category: Craig L]]
	[[Category: Guttman~~, J A~~]]		[[Category: Guttman JA]]
	[[Category: Kolappan, S]]		[[Category: Kolappan S]]
	[[Category: Lo~~, K Y~~]]		[[Category: Lo KY]]
	[[Category: Shen~~, C L.J]]~~		[[Category: Shen CLJ]]
	~~[[Category: Francisella tularensis]]~~
	~~[[Category: Unknown function]]~~
	~~[[Category: Virulence factor~~]]

OCA at 08:03, 8 January 2020

2020-01-08T08:03:00Z

← Older revision		Revision as of 08:03, 8 January 2020
Line 1:		Line 1:

	==Crystal structure of a Francisella virulence factor FvfA in the hexagonal form==		==Crystal structure of a Francisella virulence factor FvfA in the hexagonal form==
	<StructureSection load='5evf' size='340' side='right' caption='[[5evf]], [[Resolution\|resolution]] 1.76Å' scene=''>		<StructureSection load='5evf' size='340' side='right'caption='[[5evf]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"francisella_tularensis_subsp._novicida"_(larson_et_al._1955)_sjostedt_2005 "francisella tularensis subsp. novicida" (larson et al. 1955) sjostedt 2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[5evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"francisella_tularensis_subsp._novicida"_(larson_et_al._1955)_sjostedt_2005 "francisella tularensis subsp. novicida" (larson et al. 1955) sjostedt 2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EVF FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Craig, L]]		[[Category: Craig, L]]
	[[Category: Guttman, J A]]		[[Category: Guttman, J A]]

OCA at 06:35, 20 December 2017

2017-12-20T06:35:25Z

← Older revision		Revision as of 06:35, 20 December 2017
Line 3:		Line 3:
	<StructureSection load='5evf' size='340' side='right' caption='[[5evf]], [[Resolution\|resolution]] 1.76Å' scene=''>		<StructureSection load='5evf' size='340' side='right' caption='[[5evf]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5evf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EVF FirstGlance]. <br>		<table><tr><td colspan='2'>[[5evf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"francisella_tularensis_subsp._novicida"_(larson_et_al._1955)_sjostedt_2005 "francisella tularensis subsp. novicida" (larson et al. 1955) sjostedt 2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EVF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5evg\|5evg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5evg\|5evg]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACX55_1794 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5evf OCA], [http://pdbe.org/5evf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5evf RCSB], [http://www.ebi.ac.uk/pdbsum/5evf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5evf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5evf OCA], [http://pdbe.org/5evf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5evf RCSB], [http://www.ebi.ac.uk/pdbsum/5evf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5evf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Francisella tularensis is a ~~highly virulent Gram-negative intracellular~~ pathogen ~~capable of infecting a vast diversity of hosts~~, ~~ranging~~ from ~~amoebae to humans~~. ~~A hallmark of~~ F. tularensis ~~virulence is its ability to quickly grow to high densities within~~ a ~~diverse set~~ of ~~host cells, including, but not limited to,~~ macrophages ~~and epithelial cells~~. ~~We developed a luminescence reporter system~~ to ~~facilitate a large~~-~~scale transposon mutagenesis screen~~ to ~~identify genes required for growth in macrophage and epithelial cell lines~~. ~~We screened 7~~,~~454 individual mutants, 269~~ of ~~which exhibited reduced intracellular growth. Transposon insertions in~~ the ~~269 growth-defective strains mapped to 68 different genes~~. ~~FTT_0924~~, ~~a gene of unknown function but highly conserved among Francisella species~~, ~~was identified~~ in ~~this screen to be defective for intracellular growth within both macrophage and epithelial cell lines~~. FTT_0924 ~~was required for full Schu S4 virulence in~~ a ~~murine pulmonary infection model~~. ~~The DeltaFTT_0924 mutant bacterial~~ membrane ~~is permeable when replicating in hypotonic solution and within macrophages~~, ~~resulting~~ in ~~strongly reduced viability~~. The ~~permeability~~ and ~~reduced viability were rescued when the mutant was grown in a hypertonic solution, indicating that FTT_0924 is required~~ for ~~resisting osmotic stress~~. The DeltaFTT_0924 mutant was also significantly more sensitive to beta-lactam antibiotics than Schu S4. Taken together, the data strongly suggest that FTT_0924 is required for maintaining peptidoglycan integrity and virulence.		Francisella tularensis is a potent human pathogen that invades and survives in macrophage and epithelial cells. Two identical proteins, FTT_0924 from F. tularensis subsp. tularensis and FTL_1286 from F. tularensis subsp. holarctica LVS, have previously been identified as playing a role in protection of the bacteria from osmotic shock and its survival in macrophages. FTT_0924 has been shown to localize to the inner membrane, with its C-terminus exposed to the periplasm. Here, crystal structures of the F. novicida homologue FTN_0802, which we call FvfA, in two crystal forms are reported at 1.8 A resolution. FvfA differs from FTT_0924 and FTL_1286 by a single amino acid. FvfA has a DUF1471 fold that closely resembles the Escherichia coli outer membrane lipoprotein RscF, a component of a phosphorelay pathway involved in protecting bacteria from outer membrane perturbation. The structural and functional similarities and differences between these proteins and their implications for F. tularensis pathogenesis are discussed.

	~~Identifying~~ Francisella ~~tularensis genes required for growth in host cells~~.,~~Brunton J, Steele~~ S, ~~Miller C~~, ~~Lovullo E~~, ~~Taft-Benz S~~, ~~Kawula T Infect Immun~~. ~~2015 Aug~~;83(8):~~3015~~-25. doi: 10.~~1128~~/~~IAI.00004-15~~. Epub ~~2015 May, 18~~. PMID:~~25987704~~<ref>PMID:~~25987704~~</ref>		Structure of the conserved Francisella virulence protein FvfA.,Kolappan S, Lo KY, Shen CLJ, Guttman JA, Craig L Acta Crystallogr D Struct Biol. 2017 Oct 1;73(Pt 10):814-821. doi:, 10.1107/S205979831701333X. Epub 2017 Sep 27. PMID:28994410<ref>PMID:28994410</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 18:37, 26 October 2016

2016-10-26T18:37:35Z

← Older revision		Revision as of 18:37, 26 October 2016
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 5evf is ~~ON HOLD until Paper~~ Publication		==Crystal structure of a Francisella virulence factor FvfA in the hexagonal form==
			<StructureSection load='5evf' size='340' side='right' caption='[[5evf]], [[Resolution\|resolution]] 1.76Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5evf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EVF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EVF FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5evg\|5evg]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5evf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5evf OCA], [http://pdbe.org/5evf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5evf RCSB], [http://www.ebi.ac.uk/pdbsum/5evf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5evf ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Francisella tularensis is a highly virulent Gram-negative intracellular pathogen capable of infecting a vast diversity of hosts, ranging from amoebae to humans. A hallmark of F. tularensis virulence is its ability to quickly grow to high densities within a diverse set of host cells, including, but not limited to, macrophages and epithelial cells. We developed a luminescence reporter system to facilitate a large-scale transposon mutagenesis screen to identify genes required for growth in macrophage and epithelial cell lines. We screened 7,454 individual mutants, 269 of which exhibited reduced intracellular growth. Transposon insertions in the 269 growth-defective strains mapped to 68 different genes. FTT_0924, a gene of unknown function but highly conserved among Francisella species, was identified in this screen to be defective for intracellular growth within both macrophage and epithelial cell lines. FTT_0924 was required for full Schu S4 virulence in a murine pulmonary infection model. The DeltaFTT_0924 mutant bacterial membrane is permeable when replicating in hypotonic solution and within macrophages, resulting in strongly reduced viability. The permeability and reduced viability were rescued when the mutant was grown in a hypertonic solution, indicating that FTT_0924 is required for resisting osmotic stress. The DeltaFTT_0924 mutant was also significantly more sensitive to beta-lactam antibiotics than Schu S4. Taken together, the data strongly suggest that FTT_0924 is required for maintaining peptidoglycan integrity and virulence.

	~~Authors: Kolappan~~, S., Lo, ~~K.Y.~~, ~~Shen~~, C.L.J.~~, Guttman, J~~.A., ~~Craig, L~~.		Identifying Francisella tularensis genes required for growth in host cells.,Brunton J, Steele S, Miller C, Lovullo E, Taft-Benz S, Kawula T Infect Immun. 2015 Aug;83(8):3015-25. doi: 10.1128/IAI.00004-15. Epub 2015 May, 18. PMID:25987704<ref>PMID:25987704</ref>

	~~Description: Crystal structure~~ of ~~a Francisella virulence factor FvfA in~~ the ~~hexagonal form~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased Structures~~]]		</div>
			<div class="pdbe-citations 5evf" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Craig, L]]
			[[Category: Guttman, J A]]
	[[Category: Kolappan, S]]		[[Category: Kolappan, S]]
	[[Category: ~~Guttman~~, ~~J.A~~]]		[[Category: Lo, K Y]]
	[[Category: Shen, C.L.J]]		[[Category: Shen, C L.J]]
	[[Category: ~~Lo, K.Y~~]]		[[Category: Francisella tularensis]]
	[[Category: ~~Craig, L~~]]		[[Category: Unknown function]]
			[[Category: Virulence factor]]

OCA at 10:53, 1 June 2016

2016-06-01T10:53:54Z

← Older revision		Revision as of 10:53, 1 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5evf is ON HOLD		The entry 5evf is ON HOLD until Paper Publication

	Authors: Kolappan, S., Lo, K.Y., Shen, C.L.J., Guttman, J.A., Craig, L.		Authors: Kolappan, S., Lo, K.Y., Shen, C.L.J., Guttman, J.A., Craig, L.

OCA at 17:13, 30 December 2015

2015-12-30T17:13:19Z

← Older revision		Revision as of 17:13, 30 December 2015
Line 5:		Line 5:
	Authors: Kolappan, S., Lo, K.Y., Shen, C.L.J., Guttman, J.A., Craig, L.		Authors: Kolappan, S., Lo, K.Y., Shen, C.L.J., Guttman, J.A., Craig, L.

	Description: Crystal structure of a Francisella virulence factor in the hexagonal form		Description: Crystal structure of a Francisella virulence factor FvfA in the hexagonal form
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Kolappan, S]]		[[Category: Kolappan, S]]

OCA: Protected "5evf" [edit=sysop:move=sysop]

2015-12-01T03:26:18Z

Protected "5evf" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:26, 1 December 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5evf is ON HOLD Authors: Kolappan, S., Lo, K.Y., Shen, C.L.J., Guttman, J.A., Craig, L. Description: Crystal structure of a Francisella virulence f...

2015-12-01T03:26:16Z

New page: '''Unreleased structure''' The entry 5evf is ON HOLD Authors: Kolappan, S., Lo, K.Y., Shen, C.L.J., Guttman, J.A., Craig, L. Description: Crystal structure of a Francisella virulence f...

New page

'''Unreleased structure'''

The entry 5evf is ON HOLD

Authors: Kolappan, S., Lo, K.Y., Shen, C.L.J., Guttman, J.A., Craig, L.

Description: Crystal structure of a Francisella virulence factor in the hexagonal form
[[Category: Unreleased Structures]]
[[Category: Kolappan, S]]
[[Category: Guttman, J.A]]
[[Category: Shen, C.L.J]]
[[Category: Lo, K.Y]]
[[Category: Craig, L]]