5fc2 - Revision history

OCA at 06:45, 19 July 2023

2023-07-19T06:45:56Z

← Older revision		Revision as of 06:45, 19 July 2023
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	<StructureSection load='5fc2' size='340' side='right'caption='[[5fc2]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='5fc2' size='340' side='right'caption='[[5fc2]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5fc2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chatd Chatd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FC2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5fc2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FC2 FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=6L3:METHYL+2,4,6~~-~~TRIMETHYLBENZOATE'>6L3</scene>~~, ~~<scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.84Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5fc3\|5fc3]]~~<~~/td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6L3:METHYL+2,4,6-TRIMETHYLBENZOATE'>6L3</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">CTHT_0070540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fc2 OCA], [https://pdbe.org/5fc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fc2 RCSB], [https://www.ebi.ac.uk/pdbsum/5fc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fc2 ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fc2 OCA], [~~http~~://pdbe.org/5fc2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fc2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fc2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fc2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/G0SHM3_CHATD G0SHM3_CHATD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Chatd~~]]		[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lin, Z~~]]		[[Category: Synthetic construct]]
	[[Category: ~~Luo, X~~]]		[[Category: Lin Z]]
	[[Category: ~~Yu, H~~]]		[[Category: Luo X]]
	[[Category: ~~Cohesin]]~~		[[Category: Yu H]]
	~~[[Category: Complex]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Hydrolase-hydrolase inhibitor complex~~]]

OCA at 08:10, 29 April 2020

2020-04-29T08:10:11Z

← Older revision		Revision as of 08:10, 29 April 2020
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	==Structure of a separase in complex with a pAMK peptide containing a phospho-serine==		==Structure of a separase in complex with a pAMK peptide containing a phospho-serine==
	<StructureSection load='5fc2' size='340' side='right' caption='[[5fc2]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='5fc2' size='340' side='right'caption='[[5fc2]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5fc2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FC2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=5FC2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5fc2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FC2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FC2 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6L3:METHYL+2,4,6-TRIMETHYLBENZOATE'>6L3</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6L3:METHYL+2,4,6-TRIMETHYLBENZOATE'>6L3</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fc3\|5fc3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fc3\|5fc3]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fc2 OCA], [http://pdbe.org/5fc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fc2 RCSB], [http://www.ebi.ac.uk/pdbsum/5fc2 PDBsum]</span></td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0070540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fc2 OCA], [http://pdbe.org/5fc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fc2 RCSB], [http://www.ebi.ac.uk/pdbsum/5fc2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fc2 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Chatd]]
			[[Category: Large Structures]]
	[[Category: Lin, Z]]		[[Category: Lin, Z]]
	[[Category: Luo, X]]		[[Category: Luo, X]]

OCA at 18:37, 10 May 2016

2016-05-10T18:37:35Z

← Older revision		Revision as of 18:37, 10 May 2016
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 5fc2 is ~~ON HOLD until Paper~~ Publication		==Structure of a separase in complex with a pAMK peptide containing a phospho-serine==
			<StructureSection load='5fc2' size='340' side='right' caption='[[5fc2]], [[Resolution\|resolution]] 1.84Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5fc2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FC2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FC2 FirstGlance]. <br>
			</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6L3:METHYL+2,4,6-TRIMETHYLBENZOATE'>6L3</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fc3\|5fc3]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fc2 OCA], [http://pdbe.org/5fc2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fc2 RCSB], [http://www.ebi.ac.uk/pdbsum/5fc2 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Accurate chromosome segregation requires timely dissolution of chromosome cohesion after chromosomes are properly attached to the mitotic spindle. Separase is absolutely essential for cohesion dissolution in organisms from yeast to man. It cleaves the kleisin subunit of cohesin and opens the cohesin ring to allow chromosome segregation. Cohesin cleavage is spatiotemporally controlled by separase-associated regulatory proteins, including the inhibitory chaperone securin, and by phosphorylation of both the enzyme and substrates. Dysregulation of this process causes chromosome missegregation and aneuploidy, contributing to cancer and birth defects. Despite its essential functions, atomic structures of separase have not been determined. Here we report crystal structures of the separase protease domain from the thermophilic fungus Chaetomium thermophilum, alone or covalently bound to unphosphorylated and phosphorylated inhibitory peptides derived from a cohesin cleavage site. These structures reveal how separase recognizes cohesin and how cohesin phosphorylation by polo-like kinase 1 (Plk1) enhances cleavage. Consistent with a previous cellular study, mutating two securin residues in a conserved motif that partly matches the separase cleavage consensus converts securin from a separase inhibitor to a substrate. Our study establishes atomic mechanisms of substrate cleavage by separase and suggests competitive inhibition by securin.

	~~Authors:~~ Lin, Z., Luo, X., Yu, H.		Structural basis of cohesin cleavage by separase.,Lin Z, Luo X, Yu H Nature. 2016 Apr 7;532(7597):131-4. doi: 10.1038/nature17402. Epub 2016 Mar 30. PMID:27027290<ref>PMID:27027290</ref>

	~~Description: Structure~~ of ~~ctSPD~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[Category~~: ~~Unreleased Structures]]~~		</div>
	~~[[Category: Yu, H]]~~		<div class="pdbe-citations 5fc2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Lin, Z]]		[[Category: Lin, Z]]
	[[Category: Luo, X]]		[[Category: Luo, X]]
			[[Category: Yu, H]]
			[[Category: Cohesin]]
			[[Category: Complex]]
			[[Category: Hydrolase]]
			[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 10:53, 7 January 2016

2016-01-07T10:53:32Z

← Older revision		Revision as of 10:53, 7 January 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5fc2 is ON HOLD		The entry 5fc2 is ON HOLD until Paper Publication

	Authors: Lin, Z., Luo, X., Yu, H.		Authors: Lin, Z., Luo, X., Yu, H.

	Description: ~~Structural basis~~ of ~~cohesin cleavage by separase~~		Description: Structure of ctSPD
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Yu, H]]		[[Category: Yu, H]]
	[[Category: Lin, Z]]		[[Category: Lin, Z]]
	[[Category: Luo, X]]		[[Category: Luo, X]]

OCA: Protected "5fc2" [edit=sysop:move=sysop]

2015-12-23T11:26:03Z

Protected "5fc2" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:26, 23 December 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5fc2 is ON HOLD Authors: Lin, Z., Luo, X., Yu, H. Description: Structural basis of cohesin cleavage by separase Category: Unreleased Structures...

2015-12-23T11:26:02Z

New page: '''Unreleased structure''' The entry 5fc2 is ON HOLD Authors: Lin, Z., Luo, X., Yu, H. Description: Structural basis of cohesin cleavage by separase Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 5fc2 is ON HOLD

Authors: Lin, Z., Luo, X., Yu, H.

Description: Structural basis of cohesin cleavage by separase
[[Category: Unreleased Structures]]
[[Category: Yu, H]]
[[Category: Lin, Z]]
[[Category: Luo, X]]