5fml - Revision history

OCA at 06:58, 19 July 2023

2023-07-19T06:58:07Z

← Older revision		Revision as of 06:58, 19 July 2023
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	<StructureSection load='5fml' size='340' side='right'caption='[[5fml]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5fml' size='340' side='right'caption='[[5fml]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5fml]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~9infb 9infb~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FML OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5FML FirstGlance]. <br>		<table><tr><td colspan='2'>[[5fml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus Influenza B virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FML FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5fmm\|5fmm]]~~, ~~[[5fmq\|5fmq]]~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fml OCA], [~~http~~://pdbe.org/5fml PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5fml RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5fml PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5fml ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fml OCA], [https://pdbe.org/5fml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fml RCSB], [https://www.ebi.ac.uk/pdbsum/5fml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fml ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q5V8X3_9INFB Q5V8X3_9INFB]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Influenza B virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cusack, S]]		[[Category: Cusack S]]
	[[Category: Gaudon, S]]		[[Category: Gaudon S]]
	[[Category: Guilligay, D]]		[[Category: Guilligay D]]
	~~[[Category: Endonuclease]]~~
	~~[[Category: Viral protein~~]]

OCA at 07:26, 6 May 2020

2020-05-06T07:26:08Z

← Older revision		Revision as of 07:26, 6 May 2020
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	==Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide==		==Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide==
	<StructureSection load='5fml' size='340' side='right' caption='[[5fml]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5fml' size='340' side='right'caption='[[5fml]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5fml]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FML OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=5FML FirstGlance]. <br>		<table><tr><td colspan='2'>[[5fml]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9infb 9infb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FML OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FML FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fmm\|5fmm]], [[5fmq\|5fmq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fmm\|5fmm]], [[5fmq\|5fmq]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fml OCA], [http://pdbe.org/5fml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fml RCSB], [http://www.ebi.ac.uk/pdbsum/5fml PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fml OCA], [http://pdbe.org/5fml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fml RCSB], [http://www.ebi.ac.uk/pdbsum/5fml PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fml ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5fml" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5fml" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Cusack, S]]		[[Category: Cusack, S]]
	[[Category: Gaudon, S]]		[[Category: Gaudon, S]]

OCA at 20:02, 13 January 2016

2016-01-13T20:02:06Z

← Older revision		Revision as of 20:02, 13 January 2016
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	'''~~Unreleased~~ structure'''		==Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide==
			<StructureSection load='5fml' size='340' side='right' caption='[[5fml]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5fml]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FML FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5fmm\|5fmm]], [[5fmq\|5fmq]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fml OCA], [http://pdbe.org/5fml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fml RCSB], [http://www.ebi.ac.uk/pdbsum/5fml PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Influenza virus polymerase transcribes or replicates the segmented RNA genome (vRNA) into respectively viral mRNA or full-length copies and initiates RNA synthesis by binding the conserved 3' and 5' vRNA ends (the promoter). In recent structures of promoter-bound polymerase, the cap-binding and endonuclease domains are configured for cap snatching, which generates capped transcription primers. Here, we present a FluB polymerase structure with a bound complementary cRNA 5' end that exhibits a major rearrangement of the subdomains within the C-terminal two-thirds of PB2 (PB2-C). Notably, the PB2 nuclear localization signal (NLS)-containing domain translocates approximately 90 A to bind to the endonuclease domain. FluA PB2-C alone and RNA-free FluC polymerase are similarly arranged. Biophysical and cap-dependent endonuclease assays show that in solution the polymerase explores different conformational distributions depending on which RNA is bound. The inherent flexibility of the polymerase allows it to adopt alternative conformations that are likely important during polymerase maturation into active progeny RNPs.

	~~The entry 5fml is ON HOLD until Paper Publication~~		Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains.,Thierry E, Guilligay D, Kosinski J, Bock T, Gaudon S, Round A, Pflug A, Hengrung N, El Omari K, Baudin F, Hart DJ, Beck M, Cusack S Mol Cell. 2016 Jan 7;61(1):125-37. doi: 10.1016/j.molcel.2015.11.016. Epub 2015, Dec 17. PMID:26711008<ref>PMID:26711008</ref>

	~~Authors: Guilligay~~, D.~~, Gaudon,~~ S.~~, Cusack, S~~.		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~Description~~: ~~Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide~~		<div class="pdbe-citations 5fml" style="background-color:#fffaf0;"></div>
	~~[[Category: Unreleased Structures]]~~		== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Cusack, S]]		[[Category: Cusack, S]]
			[[Category: Gaudon, S]]
	[[Category: Guilligay, D]]		[[Category: Guilligay, D]]
	[[Category: ~~Gaudon, S~~]]		[[Category: Endonuclease]]
			[[Category: Viral protein]]

OCA: Protected "5fml" [edit=sysop:move=sysop]

2015-12-01T03:36:32Z

Protected "5fml" [edit=sysop:move=sysop]

← Older revision	Revision as of 03:36, 1 December 2015
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5fml is ON HOLD until Paper Publication Authors: Guilligay, D., Gaudon, S., Cusack, S. Description: Crystal structure of the endonuclease from the ...

2015-12-01T03:36:30Z

New page: '''Unreleased structure''' The entry 5fml is ON HOLD until Paper Publication Authors: Guilligay, D., Gaudon, S., Cusack, S. Description: Crystal structure of the endonuclease from the ...

New page

'''Unreleased structure'''

The entry 5fml is ON HOLD until Paper Publication

Authors: Guilligay, D., Gaudon, S., Cusack, S.

Description: Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide
[[Category: Unreleased Structures]]
[[Category: Cusack, S]]
[[Category: Guilligay, D]]
[[Category: Gaudon, S]]