5git - Revision history

OCA at 08:41, 9 April 2025

2025-04-09T08:41:45Z

← Older revision		Revision as of 08:41, 9 April 2025
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	<table><tr><td colspan='2'>[[5git]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[5git]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GIT FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>
	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XXT:[(~~3aS~~,5R,~~5aS~~,6S,8S,~~8aS~~,9S,~~9aR~~)-9-acetyloxy~~-8-hydroxy~~-5,8a-dimethyl-1-methylidene-2-~~oxo~~-4,5,5a,6,7,8,9,9a-octahydro-~~3aH~~-azuleno[6,5-b]furan-6-yl]+~~acetate~~'>XXT</scene></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XXT:[(3~{a}~{S},5~{R},5~{a}~{S},6~{S},8~{S},8~{a}~{S},9~{S},9~{a}~{R})-9-acetyloxy-5,8~{a}-dimethyl-1-methylidene-8-oxidanyl-2-oxidanylidene-4,5,5~{a},6,7,8,9,9~{a}-octahydro-3~{a}~{H}-azuleno[6,5-b]furan-6-yl]+ethanoate'>XXT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5git FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5git OCA], [https://pdbe.org/5git PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5git RCSB], [https://www.ebi.ac.uk/pdbsum/5git PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5git ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5git FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5git OCA], [https://pdbe.org/5git PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5git RCSB], [https://www.ebi.ac.uk/pdbsum/5git PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5git ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:32, 2 August 2023

2023-08-02T11:32:39Z

← Older revision		Revision as of 11:32, 2 August 2023
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	==BTB domain of KEAP1 in complex with XX3==		==BTB domain of KEAP1 in complex with XX3==
	<StructureSection load='5git' size='340' side='right' caption='[[5git]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='5git' size='340' side='right'caption='[[5git]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5git]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GIT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5GIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[5git]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GIT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XXT:[(3aS,5R,5aS,6S,8S,8aS,9S,9aR)-9-acetyloxy-8-hydroxy-5,8a-dimethyl-1-methylidene-2-oxo-4,5,5a,6,7,8,9,9a-octahydro-3aH-azuleno[6,5-b]furan-6-yl]+acetate'>XXT</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KEAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XXT:[(3aS,5R,5aS,6S,8S,8aS,9S,9aR)-9-acetyloxy-8-hydroxy-5,8a-dimethyl-1-methylidene-2-oxo-4,5,5a,6,7,8,9,9a-octahydro-3aH-azuleno[6,5-b]furan-6-yl]+acetate'>XXT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5git FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5git OCA], [~~http~~://pdbe.org/5git PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5git RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5git PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5git ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5git FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5git OCA], [https://pdbe.org/5git PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5git RCSB], [https://www.ebi.ac.uk/pdbsum/5git PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5git ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/KEAP1_HUMAN KEAP1_HUMAN]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			AIMS: Oxidative stress is considered the major cause of tissue injury after cerebral ischemia. The nuclear factor erythroid 2-related factor 2 (Nrf2) pathway is one of the most important defensive mechanisms against oxidative stresses and has been confirmed as a target for stroke treatment. Thus, we desired to find new Nrf2 activators and test their neuronal protective activity both in vivo and in vitro. RESULTS: The herb-derived compound, Britanin, is a potent inducer of the Nrf2 system. Britanin can induce the expression of protective enzymes and reverse oxygen-glucose deprivation, followed by reperfusion (OGD-R)-induced neuronal injury in primary cortical neurons in vitro. Furthermore, the administration of Britanin significantly ameliorated middle cerebral artery occlusion-reperfusion (MCAO-R) insult in vivo. We report here the crystal structure of the complex of Britanin and the BTB domain of Keap1. Britanin selectively binds to a conserved cysteine residue, cysteine 151, of Keap1 and inhibits Keap1-mediated ubiquitination of Nrf2, leading to induction of the Nrf2 pathway. INNOVATION: Britanin is a potent inducer of Nrf2. The complex crystal structure of Britanin and the BTB domain of Keap1 help clarify the mechanism of Nrf2 induction. Britanin was proven to protect primary cortical neurons against OGD-R-induced injury in an Nrf2-dependant way. Additionally, Britanin had excellent cerebroprotective effect in an MCAO-R model. CONCLUSION: Our results demonstrate that the natural product Britanin with potent Nrf2-activating and neural protective activities both in vitro and in vivo could be developed into a cerebroprotective therapeutic agent. Antioxid. Redox Signal. 27, 754-768.

			Britanin Ameliorates Cerebral Ischemia-Reperfusion Injury by Inducing the Nrf2 Protective Pathway.,Wu G, Zhu L, Yuan X, Chen H, Xiong R, Zhang S, Cheng H, Shen Y, An H, Li T, Li H, Zhang W Antioxid Redox Signal. 2017 Oct 10;27(11):754-768. doi: 10.1089/ars.2016.6885. , Epub 2017 Mar 9. PMID:28186440<ref>PMID:28186440</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5git" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Kelch-like protein 3D structures\|Kelch-like protein 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: ~~Li, H L~~]]		[[Category: Large Structures]]
	[[Category: ~~Wu, F S~~]]		[[Category: Li HL]]
	[[Category: ~~Xiong, R~~]]		[[Category: Wu FS]]
	[[Category: ~~Zhu, L L~~]]		[[Category: Xiong R]]
	[[Category: ~~Signaling protein]]~~		[[Category: Zhu LL]]
	~~[[Category: Transcription~~]]

OCA at 10:44, 6 November 2017

2017-11-06T10:44:54Z

← Older revision		Revision as of 10:44, 6 November 2017
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	~~'''Unreleased structure'''~~

	~~The entry~~ 5git is ~~ON HOLD until Paper Publication~~		==BTB domain of KEAP1 in complex with XX3==
			<StructureSection load='5git' size='340' side='right' caption='[[5git]], [[Resolution\|resolution]] 2.19Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[5git]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GIT FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XXT:[(3aS,5R,5aS,6S,8S,8aS,9S,9aR)-9-acetyloxy-8-hydroxy-5,8a-dimethyl-1-methylidene-2-oxo-4,5,5a,6,7,8,9,9a-octahydro-3aH-azuleno[6,5-b]furan-6-yl]+acetate'>XXT</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KEAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5git FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5git OCA], [http://pdbe.org/5git PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5git RCSB], [http://www.ebi.ac.uk/pdbsum/5git PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5git ProSAT]</span></td></tr>
			</table>
			__TOC__
			</StructureSection>
			[[Category: Human]]
			[[Category: Li, H L]]
			[[Category: Wu, F S]]
			[[Category: Xiong, R]]
			[[Category: Zhu, L L]]
			[[Category: Signaling protein]]
			[[Category: Transcription]]

OCA at 05:18, 12 April 2017

2017-04-12T05:18:04Z

← Older revision		Revision as of 05:18, 12 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5git is ON HOLD		The entry 5git is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5git" [edit=sysop:move=sysop]

2016-07-12T21:37:12Z

Protected "5git" [edit=sysop:move=sysop]

← Older revision	Revision as of 21:37, 12 July 2016
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5git is ON HOLD Authors: Description: Category: Unreleased Structures

2016-07-12T21:37:11Z

New page: '''Unreleased structure''' The entry 5git is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5git is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]