5j4e - Revision history

OCA at 10:47, 6 September 2023

2023-09-06T10:47:59Z

← Older revision		Revision as of 10:47, 6 September 2023
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	<StructureSection load='5j4e' size='340' side='right'caption='[[5j4e]], [[Resolution\|resolution]] 2.67Å' scene=''>		<StructureSection load='5j4e' size='340' side='right'caption='[[5j4e]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5j4e]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5J4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[5j4e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J4E FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.67Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>PP_4629 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~303~~ "~~Bacillus fluorescens putidus~~" ~~Flugge 1886])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5j4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4e OCA], [~~http~~://pdbe.org/5j4e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5j4e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5j4e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4e OCA], [https://pdbe.org/5j4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j4e RCSB], [https://www.ebi.ac.uk/pdbsum/5j4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4e ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q88E39_PSEPK Q88E39_PSEPK]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Bacillus fluorescens putidus flugge 1886]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Batra-Safferling, R~~]]		[[Category: Pseudomonas putida]]
	[[Category: ~~Granzin, J~~]]		[[Category: Batra-Safferling R]]
	[[Category: ~~Lov domain]]~~		[[Category: Granzin J]]
	~~[[Category: Signaling protein~~]]

OCA at 06:24, 16 October 2019

2019-10-16T06:24:12Z

← Older revision		Revision as of 06:24, 16 October 2019
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	==Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (Photoexcited state)==		==Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (Photoexcited state)==
	<StructureSection load='5j4e' size='340' side='right' caption='[[5j4e]], [[Resolution\|resolution]] 2.67Å' scene=''>		<StructureSection load='5j4e' size='340' side='right'caption='[[5j4e]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5j4e]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[5j4e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PP_4629 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4e OCA], [http://pdbe.org/5j4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4e RCSB], [http://www.ebi.ac.uk/pdbsum/5j4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4e OCA], [http://pdbe.org/5j4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4e RCSB], [http://www.ebi.ac.uk/pdbsum/5j4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4e ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacillus fluorescens putidus flugge 1886]]
			[[Category: Large Structures]]
	[[Category: Batra-Safferling, R]]		[[Category: Batra-Safferling, R]]
	[[Category: Granzin, J]]		[[Category: Granzin, J]]
	[[Category: Lov domain]]		[[Category: Lov domain]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 18:37, 12 July 2016

2016-07-12T18:37:51Z

← Older revision		Revision as of 18:37, 12 July 2016
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	<table><tr><td colspan='2'>[[5j4e]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[5j4e]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4e OCA], [http://pdbe.org/5j4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4e RCSB], [http://www.ebi.ac.uk/pdbsum/5j4e PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4e OCA], [http://pdbe.org/5j4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4e RCSB], [http://www.ebi.ac.uk/pdbsum/5j4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4e ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Light-Oxygen-Voltage (LOV) domains represent the photo-responsive domains of various blue-light photoreceptor proteins and are widely distributed in plants, algae, fungi, and bacteria. Here, we report the dark-state crystal structure of PpSB1-LOV, a slow-reverting short LOV protein from Pseudomonas putida that is remarkably different from our previously published "fully light-adapted" structure [1]. A direct comparison of the two structures provides insight into the light-activated signaling mechanism. Major structural differences involve a~11A movement of the C terminus in helix Jalpha, ~4A movement of Hbeta-Ibeta loop, disruption of hydrogen bonds in the dimer interface, and a~29 degrees rotation of chain-B relative to chain-A as compared to the light-state dimer. Both crystal structures and solution NMR data are suggestive of the key roles of a conserved glutamine Q116 and the N-cap region consisting of A'alpha-Abeta loop and the A'alpha helix in controlling the light-activated conformational changes. The activation mechanism proposed here for the PpSB1-LOV supports a rotary switch mechanism and provides insights into the signal propagation mechanism in naturally existing and artificial LOV-based, two-component systems and regulators.

			Signaling States of a Short Blue-Light Photoreceptor Protein PpSB1-LOV Revealed from Crystal Structures and Solution NMR Spectroscopy.,Rollen K, Granzin J, Panwalkar V, Arinkin V, Rani R, Hartmann R, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R J Mol Biol. 2016 Jun 9. pii: S0022-2836(16)30206-6. doi:, 10.1016/j.jmb.2016.05.027. PMID:27291287<ref>PMID:27291287</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5j4e" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 23:28, 23 June 2016

2016-06-23T23:28:41Z

← Older revision		Revision as of 23:28, 23 June 2016
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	~~'''Unreleased structure'''~~

	~~The entry~~ 5j4e is ~~ON HOLD until Paper Publication~~		==Crystal structures reveal signaling states of a short blue light photoreceptor protein PpSB1-LOV (Photoexcited state)==
			<StructureSection load='5j4e' size='340' side='right' caption='[[5j4e]], [[Resolution\|resolution]] 2.67Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[5j4e]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4E FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4e OCA], [http://pdbe.org/5j4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4e RCSB], [http://www.ebi.ac.uk/pdbsum/5j4e PDBsum]</span></td></tr>
			</table>
			__TOC__
			</StructureSection>
			[[Category: Batra-Safferling, R]]
			[[Category: Granzin, J]]
			[[Category: Lov domain]]
			[[Category: Signaling protein]]

OCA at 15:02, 15 May 2016

2016-05-15T15:02:22Z

← Older revision		Revision as of 15:02, 15 May 2016
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	The entry 5j4e is ON HOLD until Paper Publication		The entry 5j4e is ON HOLD until Paper Publication

	Authors: ~~Granzin, J., Batra-Safferling, R.~~		Authors:

	Description:		Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	~~[[Category: Batra-Safferling, R]]~~
	~~[[Category: Granzin, J]]~~

OCA: Protected "5j4e" [edit=sysop:move=sysop]

2016-05-11T01:26:32Z

Protected "5j4e" [edit=sysop:move=sysop]

← Older revision	Revision as of 01:26, 11 May 2016
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5j4e is ON HOLD until Paper Publication Authors: Granzin, J., Batra-Safferling, R. Description: Category: Unreleased Structures [[Category: Ba...

2016-05-11T01:26:30Z

New page: '''Unreleased structure''' The entry 5j4e is ON HOLD until Paper Publication Authors: Granzin, J., Batra-Safferling, R. Description: Category: Unreleased Structures [[Category: Ba...

New page

'''Unreleased structure'''

The entry 5j4e is ON HOLD until Paper Publication

Authors: Granzin, J., Batra-Safferling, R.

Description:
[[Category: Unreleased Structures]]
[[Category: Batra-Safferling, R]]
[[Category: Granzin, J]]