5num - Revision history

OCA at 11:50, 1 February 2024

2024-02-01T11:50:41Z

← Older revision		Revision as of 11:50, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5num]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUM FirstGlance]. <br>		<table><tr><td colspan='2'>[[5num]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5num OCA], [https://pdbe.org/5num PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5num RCSB], [https://www.ebi.ac.uk/pdbsum/5num PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5num ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5num OCA], [https://pdbe.org/5num PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5num RCSB], [https://www.ebi.ac.uk/pdbsum/5num PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5num ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:25, 7 December 2022

2022-12-07T08:25:23Z

← Older revision		Revision as of 08:25, 7 December 2022
Line 1:		Line 1:

	==Engineered beta-lactoglobulin: variant F105L-L39A in complex with chlorpromazine (LG-LA-CLP)==		==Engineered beta-lactoglobulin: variant F105L-L39A in complex with chlorpromazine (LG-LA-CLP)==
	<StructureSection load='5num' size='340' side='right' caption='[[5num]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5num' size='340' side='right'caption='[[5num]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5num]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NUM FirstGlance]. <br>		<table><tr><td colspan='2'>[[5num]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5num OCA], [https://pdbe.org/5num PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5num RCSB], [https://www.ebi.ac.uk/pdbsum/5num PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5num ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5num OCA], [~~http~~://pdbe.org/5num PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5num RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5num PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5num ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.		[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5num" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5num" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Beta-lactoglobulin 3D structures\|Beta-lactoglobulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bovin~~]]		[[Category: Bos taurus]]
	[[Category: Bonarek, P]]		[[Category: Large Structures]]
	[[Category: Lazinska, I]]		[[Category: Bonarek P]]
	[[Category: Lewinski, K]]		[[Category: Lazinska I]]
	[[Category: Loch~~, J I~~]]		[[Category: Lewinski K]]
	[[Category: Szydlowska, J]]		[[Category: Loch JI]]
	[[Category: Tworzydlo, M]]		[[Category: Szydlowska J]]
	~~[[Category: Beta-lactoglobulin]]~~		[[Category: Tworzydlo M]]
	~~[[Category: Complex]]~~
	~~[[Category: Lipocalin]]~~
	~~[[Category: Mutant]]~~
	~~[[Category: Transport protein~~]]

OCA at 06:27, 18 April 2018

2018-04-18T06:27:23Z

← Older revision		Revision as of 06:27, 18 April 2018
Line 3:		Line 3:
	<StructureSection load='5num' size='340' side='right' caption='[[5num]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5num' size='340' side='right' caption='[[5num]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5num]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUM FirstGlance]. <br>		<table><tr><td colspan='2'>[[5num]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5num OCA], [http://pdbe.org/5num PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5num RCSB], [http://www.ebi.ac.uk/pdbsum/5num PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5num ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5num OCA], [http://pdbe.org/5num PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5num RCSB], [http://www.ebi.ac.uk/pdbsum/5num PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5num ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.		[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Chlorpromazine (CPZ) is a phenothiazine acting as dopamine antagonist. Aside from application in schizophrenia therapy, chlorpromazine is found to be a putative inhibitor of proteins involved in cancers, heritable autism disorder and prion diseases. Four new beta-lactoglobulin variants with double or triple substitutions: I56F/L39A, F105L/L39A, I56F/L39A/M107F or F105L/L39A/M107F changing the shape of the binding pocket were produced and their chlorpromazine binding properties have been investigated by X-ray crystallography, circular dichroism, isothermal titration calorimetry and thermophoresis. The CD spectra and crystal structures revealed that mutations do not affect the protein overall structure but in comparison to WT protein, variants possessing I56F substitution had lower stability while mutation F105L increased melting temperature of the protein. The new variants showed affinity to chlorpromazine in the range 4.2-15.4x10(3)M(-1). The CD spectra and crystal structures revealed complementarity of the binding pocket shape, to only one chlorpromazine chiral conformer. The (aR)-CPZ was bonded to variants containing I56F substitution while variants with F105L substitution preferred (aS)-CPZ.

			The engineered beta-lactoglobulin with complementarity to the chlorpromazine chiral conformers.,Loch JI, Bonarek P, Tworzydlo M, Lazinska I, Szydlowska J, Lipowska J, Rzesikowska K, Lewinski K Int J Biol Macromol. 2018 Mar 16;114:85-96. doi: 10.1016/j.ijbiomac.2018.03.074. PMID:29555509<ref>PMID:29555509</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5num" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bovin]]
	[[Category: Bonarek, P]]		[[Category: Bonarek, P]]
	[[Category: Lazinska, I]]		[[Category: Lazinska, I]]

OCA at 05:51, 4 April 2018

2018-04-04T05:51:45Z

← Older revision		Revision as of 05:51, 4 April 2018
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 5num ~~is ON HOLD until Paper Publication~~		==Engineered beta-lactoglobulin: variant F105L-L39A in complex with chlorpromazine (LG-LA-CLP)==
			<StructureSection load='5num' size='340' side='right' caption='[[5num]], [[Resolution\|resolution]] 2.30Å' scene=''>
	~~Authors~~: ~~Loch, J~~.I., ~~Bonarek~~, P., ~~Tworzydlo~~, M., ~~Lazinska~~, I., ~~Szydlowska, J~~.~~, Lewinski, K~~.		== Structural highlights ==
			<table><tr><td colspan='2'>[[5num]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUM FirstGlance]. <br>
	~~Description: Engineered beta-lactoglobulin: variant F105L-L39A in complex with chlorpromazine (LG-LA-CLP)~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5num FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5num OCA], [http://pdbe.org/5num PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5num RCSB], [http://www.ebi.ac.uk/pdbsum/5num PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5num ProSAT]</span></td></tr>
	[[Category: ~~Loch~~, ~~J.I~~]]		</table>
	[[Category: ~~Tworzydlo~~, M]]		== Function ==
			[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
			__TOC__
			</StructureSection>
			[[Category: Bonarek, P]]
			[[Category: Lazinska, I]]
	[[Category: Lewinski, K]]		[[Category: Lewinski, K]]
	[[Category: ~~Lazinska~~, I]]		[[Category: Loch, J I]]
	~~[[Category: Bonarek, P~~]]
	[[Category: Szydlowska, J]]		[[Category: Szydlowska, J]]
			[[Category: Tworzydlo, M]]
			[[Category: Beta-lactoglobulin]]
			[[Category: Complex]]
			[[Category: Lipocalin]]
			[[Category: Mutant]]
			[[Category: Transport protein]]

OCA: Protected "5num" [edit=sysop:move=sysop]

2017-05-10T09:22:13Z

Protected "5num" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:22, 10 May 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5num is ON HOLD until Paper Publication Authors: Loch, J.I., Bonarek, P., Tworzydlo, M., Lazinska, I., Szydlowska, J., Lewinski, K. Description: En...

2017-05-10T09:22:11Z

New page: '''Unreleased structure''' The entry 5num is ON HOLD until Paper Publication Authors: Loch, J.I., Bonarek, P., Tworzydlo, M., Lazinska, I., Szydlowska, J., Lewinski, K. Description: En...

New page

'''Unreleased structure'''

The entry 5num is ON HOLD until Paper Publication

Authors: Loch, J.I., Bonarek, P., Tworzydlo, M., Lazinska, I., Szydlowska, J., Lewinski, K.

Description: Engineered beta-lactoglobulin: variant F105L-L39A in complex with chlorpromazine (LG-LA-CLP)
[[Category: Unreleased Structures]]
[[Category: Loch, J.I]]
[[Category: Tworzydlo, M]]
[[Category: Lewinski, K]]
[[Category: Lazinska, I]]
[[Category: Bonarek, P]]
[[Category: Szydlowska, J]]