5o7b - Revision history

OCA at 19:10, 29 November 2023

2023-11-29T19:10:22Z

← Older revision		Revision as of 19:10, 29 November 2023
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	<StructureSection load='5o7b' size='340' side='right'caption='[[5o7b]], [[Resolution\|resolution]] 2.28Å' scene=''>		<StructureSection load='5o7b' size='340' side='right'caption='[[5o7b]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5o7b]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Syny3 Syny3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O7B FirstGlance]. <br>		<table><tr><td colspan='2'>[[5o7b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O7B FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>slr0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=1111708 SYNY3])</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.281Å</td></tr>
	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class~~="~~sblockDat~~">~~<span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine~~-~~phosphatase]~~, ~~with EC number~~ [~~http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7b OCA], [https://pdbe.org/5o7b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o7b RCSB], [https://www.ebi.ac.uk/pdbsum/5o7b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7b ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7b OCA], [~~http~~://pdbe.org/5o7b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o7b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o7b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7b ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Y328_SYNY3 Y328_SYNY3]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Protein-tyrosine-phosphatase]]~~		[[Category: Synechocystis sp. PCC 6803 substr. Kazusa]]
	~~[[Category: Syny3~~]]		[[Category: Gales L]]
	[[Category: Gales, L]]		[[Category: Leite JP]]
	[[Category: Leite~~, J P]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Phosphatase~~]]

OCA at 08:14, 20 March 2019

2019-03-20T08:14:13Z

← Older revision		Revision as of 08:14, 20 March 2019
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	==CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM SYNECHOCYSTIS SP. PCC 6803==		==CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM SYNECHOCYSTIS SP. PCC 6803==
	<StructureSection load='5o7b' size='340' side='right' caption='[[5o7b]], [[Resolution\|resolution]] 2.28Å' scene=''>		<StructureSection load='5o7b' size='340' side='right'caption='[[5o7b]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5o7b]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7B FirstGlance]. <br>		<table><tr><td colspan='2'>[[5o7b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7B FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">slr0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7b OCA], [http://pdbe.org/5o7b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7b RCSB], [http://www.ebi.ac.uk/pdbsum/5o7b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7b OCA], [http://pdbe.org/5o7b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7b RCSB], [http://www.ebi.ac.uk/pdbsum/5o7b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7b ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Many cyanobacteria produce extracellular polymeric substances (EPS) mainly composed of heteropolysaccharides with unique characteristics that make them suitable for biotechnological applications. However, manipulation/optimization of EPS biosynthesis/characteristics is hindered by a poor understanding of the production pathways and the differences between bacterial species. In this work, genes putatively related to different pathways of cyanobacterial EPS polymerization, assembly, and export were targeted for deletion or truncation in the unicellular Synechocystis sp. PCC 6803. No evident phenotypic changes were observed for some mutants in genes occurring in multiple copies in Synechocystis genome, namely wzy (sll0737), wzx (sll5049), kpsM (slr2107), and kpsMwzy (slr2107sll0737), strongly suggesting functional redundancy. In contrast, Deltawzc (Deltasll0923) and Deltawzb (Deltaslr0328) influenced both the amount and composition of the EPS, establishing that Wzc participates in the production of capsular (CPS) and released (RPS) polysaccharides, and Wzb affects RPS production. The structure of Wzb was solved (2.28 A), revealing structural differences relative to other phosphatases involved in EPS production and suggesting a different substrate recognition mechanism. In addition, Wzc showed the ATPase and autokinase activities typical of bacterial tyrosine kinases. Most importantly, Wzb was able to dephosphorylate Wzc in vitro, suggesting that tyrosine phosphorylation/dephosphorylation plays a role in cyanobacterial EPS production.

			The role of the tyrosine kinase Wzc (Sll0923) and the phosphatase Wzb (Slr0328) in the production of extracellular polymeric substances (EPS) by Synechocystis PCC 6803.,Pereira SB, Santos M, Leite JP, Flores C, Eisfeld C, Buttel Z, Mota R, Rossi F, De Philippis R, Gales L, Morais-Cabral JH, Tamagnini P Microbiologyopen. 2019 Jan 23:e753. doi: 10.1002/mbo3.753. PMID:30675753<ref>PMID:30675753</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5o7b" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Protein-tyrosine-phosphatase]]		[[Category: Protein-tyrosine-phosphatase]]
			[[Category: Syny3]]
	[[Category: Gales, L]]		[[Category: Gales, L]]
	[[Category: Leite, J P]]		[[Category: Leite, J P]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphatase]]		[[Category: Phosphatase]]

OCA at 05:37, 20 June 2018

2018-06-20T05:37:51Z

← Older revision		Revision as of 05:37, 20 June 2018
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	~~'''Unreleased structure'''~~

	~~The entry 5o7b is ON HOLD until Paper Publication~~		==CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM SYNECHOCYSTIS SP. PCC 6803==
			<StructureSection load='5o7b' size='340' side='right' caption='[[5o7b]], [[Resolution\|resolution]] 2.28Å' scene=''>
	~~Authors: Leite, J.P., Gales, L.~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[5o7b]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7B FirstGlance]. <br>
	~~Description:~~ CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM SYNECHOCYSTIS SP. PCC 6803		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7b OCA], [http://pdbe.org/5o7b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7b RCSB], [http://www.ebi.ac.uk/pdbsum/5o7b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7b ProSAT]</span></td></tr>
	[[~~Category~~: ~~Leite~~, J.P]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Protein-tyrosine-phosphatase]]
	[[Category: Gales, L]]		[[Category: Gales, L]]
			[[Category: Leite, J P]]
			[[Category: Hydrolase]]
			[[Category: Phosphatase]]

OCA: Protected "5o7b" [edit=sysop:move=sysop]

2017-09-27T12:06:35Z

Protected "5o7b" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:06, 27 September 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5o7b is ON HOLD until Paper Publication Authors: Leite, J.P., Gales, L. Description: CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM...

2017-09-27T12:06:34Z

New page: '''Unreleased structure''' The entry 5o7b is ON HOLD until Paper Publication Authors: Leite, J.P., Gales, L. Description: CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM...

New page

'''Unreleased structure'''

The entry 5o7b is ON HOLD until Paper Publication

Authors: Leite, J.P., Gales, L.

Description: CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM SYNECHOCYSTIS SP. PCC 6803
[[Category: Unreleased Structures]]
[[Category: Leite, J.P]]
[[Category: Gales, L]]