5o7w - Revision history

OCA at 19:10, 29 November 2023

2023-11-29T19:10:49Z

← Older revision		Revision as of 19:10, 29 November 2023
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	==Crystal structure of the 4-FLUORO RSL lectin in complex with Lewis x tetrasaccharide==		==Crystal structure of the 4-FLUORO RSL lectin in complex with Lewis x tetrasaccharide==
	<StructureSection load='5o7w' size='340' side='right' caption='[[5o7w]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='5o7w' size='340' side='right'caption='[[5o7w]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5o7w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_solanacearum"_smith_1896 "bacillus~~ solanacearum~~" smith 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O7W FirstGlance]. <br>		<table><tr><td colspan='2'>[[5o7w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O7W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.35Å</td></tr>
	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4FW:4-FLUOROTRYPTOPHANE'>4FW</scene></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4FW:4-FLUOROTRYPTOPHANE'>4FW</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CMR15_11270 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7w OCA], [https://pdbe.org/5o7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o7w RCSB], [https://www.ebi.ac.uk/pdbsum/5o7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7w ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7w OCA], [~~http~~://pdbe.org/5o7w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o7w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o7w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7w ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A0S4TLR1_RALSL A0A0S4TLR1_RALSL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus solanacearum smith 1896~~]]		[[Category: Large Structures]]
	[[Category: ~~Varrot, A~~]]		[[Category: Ralstonia solanacearum]]
	[[Category: ~~Carbohydrate]]~~		[[Category: Varrot A]]
	~~[[Category: Fluorinated tryptophan]]~~
	~~[[Category: Lectin]]~~
	~~[[Category: Lewis x]]~~
	~~[[Category: Sugar binding protein~~]]

OCA at 08:01, 14 June 2018

2018-06-14T08:01:57Z

← Older revision		Revision as of 08:01, 14 June 2018
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	<StructureSection load='5o7w' size='340' side='right' caption='[[5o7w]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='5o7w' size='340' side='right' caption='[[5o7w]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5o7w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7W FirstGlance]. <br>		<table><tr><td colspan='2'>[[5o7w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4FW:4-FLUOROTRYPTOPHANE'>4FW</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4FW:4-FLUOROTRYPTOPHANE'>4FW</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CMR15_11270 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7w OCA], [http://pdbe.org/5o7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7w RCSB], [http://www.ebi.ac.uk/pdbsum/5o7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7w OCA], [http://pdbe.org/5o7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7w RCSB], [http://www.ebi.ac.uk/pdbsum/5o7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7w ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Protein-carbohydrate interactions play crucial roles in biology. Understanding and modifying these interactions is of major interest for fighting many diseases. We took a synthetic biology approach and incorporated noncanonical amino acids into a bacterial lectin to modulate its interactions with carbohydrates. We focused on tryptophan, which is prevalent in carbohydrate binding sites. The exchange of the tryptophan residues with analogs fluorinated at different positions resulted in three distinctly fluorinated variants of the lectin from Ralstonia solanacearum. We observed differences in stability and affinity toward fucosylated glycans and rationalized them by X-ray and modeling studies. While fluorination decreased the aromaticity of the indole ring and, therefore, the strength of carbohydrate-aromatic interactions, additional weak hydrogen bonds were formed between fluorine and the ligand hydroxyl groups. Our approach opens new possibilities to engineer carbohydrate receptors.

			Effect of Noncanonical Amino Acids on Protein-Carbohydrate Interactions: Structure, Dynamics, and Carbohydrate Affinity of a Lectin Engineered with Fluorinated Tryptophan Analogs.,Tobola F, Lelimousin M, Varrot A, Gillon E, Darnhofer B, Blixt O, Birner-Gruenberger R, Imberty A, Wiltschi B ACS Chem Biol. 2018 Jun 12. doi: 10.1021/acschembio.8b00377. PMID:29812892<ref>PMID:29812892</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5o7w" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacillus solanacearum smith 1896]]
	[[Category: Varrot, A]]		[[Category: Varrot, A]]
	[[Category: Carbohydrate]]		[[Category: Carbohydrate]]

OCA at 05:48, 6 June 2018

2018-06-06T05:48:18Z

← Older revision		Revision as of 05:48, 6 June 2018
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	~~'''Unreleased structure'''~~

	~~The entry 5o7w is ON HOLD until Paper Publication~~		==Crystal structure of the 4-FLUORO RSL lectin in complex with Lewis x tetrasaccharide==
			<StructureSection load='5o7w' size='340' side='right' caption='[[5o7w]], [[Resolution\|resolution]] 1.35Å' scene=''>
	~~Authors: Varrot, A.~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[5o7w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7W FirstGlance]. <br>
	~~Description:~~ Crystal structure of the 4-FLUORO RSL lectin in complex with Lewis x tetrasaccharide		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4FW:4-FLUOROTRYPTOPHANE'>4FW</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7w OCA], [http://pdbe.org/5o7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7w RCSB], [http://www.ebi.ac.uk/pdbsum/5o7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7w ProSAT]</span></td></tr>
			</table>
			__TOC__
			</StructureSection>
	[[Category: Varrot, A]]		[[Category: Varrot, A]]
			[[Category: Carbohydrate]]
			[[Category: Fluorinated tryptophan]]
			[[Category: Lectin]]
			[[Category: Lewis x]]
			[[Category: Sugar binding protein]]

OCA: Protected "5o7w" [edit=sysop:move=sysop]

2017-09-27T12:07:31Z

Protected "5o7w" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:07, 27 September 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5o7w is ON HOLD until Paper Publication Authors: Varrot, A. Description: Crystal structure of the 4-FLUORO RSL lectin in complex with Lewis x tetra...

2017-09-27T12:07:30Z

New page: '''Unreleased structure''' The entry 5o7w is ON HOLD until Paper Publication Authors: Varrot, A. Description: Crystal structure of the 4-FLUORO RSL lectin in complex with Lewis x tetra...

New page

'''Unreleased structure'''

The entry 5o7w is ON HOLD until Paper Publication

Authors: Varrot, A.

Description: Crystal structure of the 4-FLUORO RSL lectin in complex with Lewis x tetrasaccharide
[[Category: Unreleased Structures]]
[[Category: Varrot, A]]