5oad - Revision history

OCA at 09:38, 6 December 2023

2023-12-06T09:38:19Z

← Older revision		Revision as of 09:38, 6 December 2023
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	<StructureSection load='5oad' size='340' side='right'caption='[[5oad]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5oad' size='340' side='right'caption='[[5oad]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5oad]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Aplca Aplca~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OAD FirstGlance]. <br>		<table><tr><td colspan='2'>[[5oad]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OAD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5oad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oad OCA], [~~http~~://pdbe.org/5oad PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5oad RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5oad PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5oad ProSAT]</span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oad OCA], [https://pdbe.org/5oad PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oad RCSB], [https://www.ebi.ac.uk/pdbsum/5oad PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oad ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q8WSF8_APLCA Q8WSF8_APLCA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5oad" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5oad" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Acetylcholine binding protein 3D structures\|Acetylcholine binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Aplca~~]]		[[Category: Aplysia californica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Dawson, A]]		[[Category: Dawson A]]
	[[Category: Hunter~~, W N]]~~		[[Category: Hunter WN]]
	~~[[Category: Souza, J O.de~~]]		[[Category: Trumper P]]
	[[Category: Trumper, P]]		[[Category: De Souza JO]]
	[[Category: ~~Acetylcholine binding]]~~
	~~[[Category: Receptor]]~~
	~~[[Category: Signaling protein~~]]

OCA at 04:34, 13 February 2020

2020-02-13T04:34:33Z

← Older revision		Revision as of 04:34, 13 February 2020
Line 1:		Line 1:

	==Crystal structure of mutant AChBP in complex with HEPES (T53F, Q74R, Y110A, I135S, G162E)==		==Crystal structure of mutant AChBP in complex with HEPES (T53F, Q74R, Y110A, I135S, G162E)==
	<StructureSection load='5oad' size='340' side='right' caption='[[5oad]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5oad' size='340' side='right'caption='[[5oad]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5oad]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OAD FirstGlance]. <br>		<table><tr><td colspan='2'>[[5oad]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Aplca Aplca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OAD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oad OCA], [http://pdbe.org/5oad PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oad RCSB], [http://www.ebi.ac.uk/pdbsum/5oad PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oad ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oad OCA], [http://pdbe.org/5oad PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oad RCSB], [http://www.ebi.ac.uk/pdbsum/5oad PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oad ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Protein-engineering methods have been exploited to produce a surrogate system for the extracellular neurotransmitter-binding site of a heteromeric human ligand-gated ion channel, the glycine receptor. This approach circumvents two major issues: the inherent experimental difficulties in working with a membrane-bound ion channel and the complication that a heteromeric assembly is necessary to create a key, physiologically relevant binding site. Residues that form the orthosteric site in a highly stable ortholog, acetylcholine-binding protein, were selected for substitution. Recombinant proteins were prepared and characterized in stepwise fashion exploiting a range of biophysical techniques, including X-ray crystallography, married to the use of selected chemical probes. The decision making and development of the surrogate, which is termed a glycine-binding protein, are described, and comparisons are provided with wild-type and homomeric systems that establish features of molecular recognition in the binding site and the confidence that the system is suited for use in early-stage drug discovery targeting a heteromeric alpha/beta glycine receptor.

			Engineering a surrogate human heteromeric alpha/beta glycine receptor orthosteric site exploiting the structural homology and stability of acetylcholine-binding protein.,Dawson A, Trumper P, de Souza JO, Parker H, Jones MJ, Hales TG, Hunter WN IUCrJ. 2019 Sep 4;6(Pt 6):1014-1023. doi: 10.1107/S205225251901114X. eCollection , 2019 Nov 1. PMID:31709057<ref>PMID:31709057</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5oad" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Aplca]]
			[[Category: Large Structures]]
	[[Category: Dawson, A]]		[[Category: Dawson, A]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 18:33, 1 August 2018

2018-08-01T18:33:38Z

← Older revision		Revision as of 18:33, 1 August 2018
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	~~'''Unreleased structure'''~~

	~~The entry 5oad is ON HOLD until Paper Publication~~		==Crystal structure of mutant AChBP in complex with HEPES (T53F, Q74R, Y110A, I135S, G162E)==
			<StructureSection load='5oad' size='340' side='right' caption='[[5oad]], [[Resolution\|resolution]] 2.10Å' scene=''>
	~~Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P.~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[5oad]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OAD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OAD FirstGlance]. <br>
	~~Description:~~ Crystal structure of mutant AChBP in complex with HEPES (T53F, Q74R, Y110A, I135S, G162E)		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oad FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oad OCA], [http://pdbe.org/5oad PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oad RCSB], [http://www.ebi.ac.uk/pdbsum/5oad PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oad ProSAT]</span></td></tr>
	[[~~Category~~: ~~Hunter~~, W.N]]		</table>
	[[~~Category~~: ~~De Souza~~, J.O]]		__TOC__
			</StructureSection>
	[[Category: Dawson, A]]		[[Category: Dawson, A]]
			[[Category: Hunter, W N]]
			[[Category: Souza, J O.de]]
	[[Category: Trumper, P]]		[[Category: Trumper, P]]
			[[Category: Acetylcholine binding]]
			[[Category: Receptor]]
			[[Category: Signaling protein]]

OCA: Protected "5oad" [edit=sysop:move=sysop]

2017-09-27T12:12:35Z

Protected "5oad" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:12, 27 September 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5oad is ON HOLD until Paper Publication Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P. Description: Crystal structure of mutant ACh...

2017-09-27T12:12:34Z

New page: '''Unreleased structure''' The entry 5oad is ON HOLD until Paper Publication Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P. Description: Crystal structure of mutant ACh...

New page

'''Unreleased structure'''

The entry 5oad is ON HOLD until Paper Publication

Authors: Dawson, A., Hunter, W.N., de Souza, J.O., Trumper, P.

Description: Crystal structure of mutant AChBP in complex with HEPES (T53F, Q74R, Y110A, I135S, G162E)
[[Category: Unreleased Structures]]
[[Category: Hunter, W.N]]
[[Category: De Souza, J.O]]
[[Category: Dawson, A]]
[[Category: Trumper, P]]