5opy - Revision history

OCA at 11:22, 3 January 2024

2024-01-03T11:22:34Z

← Older revision		Revision as of 11:22, 3 January 2024
Line 3:		Line 3:
	<StructureSection load='5opy' size='340' side='right'caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='5opy' size='340' side='right'caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OPY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [https://pdbe.org/5opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [https://www.ebi.ac.uk/pdbsum/5opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [https://pdbe.org/5opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [https://www.ebi.ac.uk/pdbsum/5opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Backovic, M]]		[[Category: Backovic M]]
	[[Category: Borst~~, A J~~]]		[[Category: Borst AJ]]
	[[Category: DiMaio, F]]		[[Category: DiMaio F]]
	[[Category: Ginsberg, M]]		[[Category: Ginsberg M]]
	[[Category: James~~, Z M~~]]		[[Category: James ZM]]
	[[Category: Rey~~, F A~~]]		[[Category: Rey FA]]
	[[Category: Veesler, D]]		[[Category: Veesler D]]
	[[Category: Zagotta, W]]		[[Category: Zagotta W]]
	~~[[Category: Antigen-binding fragment]]~~
	~~[[Category: Fab]]~~
	~~[[Category: Immune system]]~~
	~~[[Category: Lm609~~]]

OCA at 10:56, 30 March 2022

2022-03-30T10:56:38Z

← Older revision		Revision as of 10:56, 30 March 2022
Line 3:		Line 3:
	<StructureSection load='5opy' size='340' side='right'caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='5opy' size='340' side='right'caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5OPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OPY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [~~http~~://pdbe.org/5opy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5opy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [https://pdbe.org/5opy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [https://www.ebi.ac.uk/pdbsum/5opy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:30, 2 September 2020

2020-09-02T07:30:29Z

← Older revision		Revision as of 07:30, 2 September 2020
Line 1:		Line 1:

	==Crystal structure of anti-alphaVbeta3 integrin Fab LM609==		==Crystal structure of anti-alphaVbeta3 integrin Fab LM609==
	<StructureSection load='5opy' size='340' side='right' caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='5opy' size='340' side='right'caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OPY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [http://pdbe.org/5opy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [http://www.ebi.ac.uk/pdbsum/5opy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [http://pdbe.org/5opy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [http://www.ebi.ac.uk/pdbsum/5opy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Backovic, M]]		[[Category: Backovic, M]]

OCA at 10:14, 15 November 2017

2017-11-15T10:14:03Z

← Older revision		Revision as of 10:14, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5opy' size='340' side='right' caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='5opy' size='340' side='right' caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPY FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [http://pdbe.org/5opy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [http://www.ebi.ac.uk/pdbsum/5opy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [http://pdbe.org/5opy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [http://www.ebi.ac.uk/pdbsum/5opy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>
	</table>		</table>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Lk3 transgenic mice]]
	[[Category: Backovic, M]]		[[Category: Backovic, M]]
	[[Category: Borst, A J]]		[[Category: Borst, A J]]

OCA at 06:58, 25 October 2017

2017-10-25T06:58:09Z

← Older revision		Revision as of 06:58, 25 October 2017
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 5opy is ~~ON HOLD until Paper~~ Publication		==Crystal structure of anti-alphaVbeta3 integrin Fab LM609==
			<StructureSection load='5opy' size='340' side='right' caption='[[5opy]], [[Resolution\|resolution]] 2.26Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5opy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OPY FirstGlance]. <br>
			</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5opy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5opy OCA], [http://pdbe.org/5opy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5opy RCSB], [http://www.ebi.ac.uk/pdbsum/5opy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5opy ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The LM609 antibody specifically recognizes alphaVbeta3 integrin and inhibits angiogenesis, bone resorption, and viral infections in an arginine-glycine-aspartate-independent manner. LM609 entered phase II clinical trials for the treatment of several cancers and was also used for alphaVbeta3-targeted radioimmunotherapy. To elucidate the mechanisms of recognition and inhibition of alphaVbeta3 integrin, we solved the structure of the LM609 antigen-binding fragment by X-ray crystallography and determined its binding affinity for alphaVbeta3. Using single-particle electron microscopy, we show that LM609 binds at the interface between the beta-propeller domain of the alphaV chain and the betaI domain of the beta3 chain, near the RGD-binding site, of all observed integrin conformational states. Integrating these data with fluorescence size-exclusion chromatography, we demonstrate that LM609 sterically hinders access of large ligands to the RGD-binding pocket, without obstructing it. This work provides a structural framework to expedite future efforts utilizing LM609 as a diagnostic or therapeutic tool.

	~~Authors: Backovic, M., Veesler, D~~., Borst~~, A.J.~~, James~~, Z.M.~~, Zagotta~~, W.~~, Ginsberg, M., Rey, F.A., ~~DiMaio, F~~.		The Therapeutic Antibody LM609 Selectively Inhibits Ligand Binding to Human alphaVbeta3 Integrin via Steric Hindrance.,Borst AJ, James ZM, Zagotta WN, Ginsberg M, Rey FA, DiMaio F, Backovic M, Veesler D Structure. 2017 Oct 7. pii: S0969-2126(17)30298-8. doi:, 10.1016/j.str.2017.09.007. PMID:29033288<ref>PMID:29033288</ref>

	~~Description: Crystal structure~~ of ~~anti~~-~~alphaVbeta3 integrin Fab LM609~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[Category: Unreleased Structures]]~~		</div>
	~~[[Category: Zagotta, W]]~~		<div class="pdbe-citations 5opy" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Backovic, M]]		[[Category: Backovic, M]]
			[[Category: Borst, A J]]
			[[Category: DiMaio, F]]
			[[Category: Ginsberg, M]]
			[[Category: James, Z M]]
			[[Category: Rey, F A]]
	[[Category: Veesler, D]]		[[Category: Veesler, D]]
	[[Category: ~~James~~, ~~Z.M~~]]		[[Category: Zagotta, W]]
	[[Category: ~~Ginsberg, M~~]]		[[Category: Antigen-binding fragment]]
	[[Category: ~~Borst, A.J~~]]		[[Category: Fab]]
	[[Category: ~~Rey, F.A~~]]		[[Category: Immune system]]
	[[Category: ~~Dimaio, F~~]]		[[Category: Lm609]]

OCA: Protected "5opy" [edit=sysop:move=sysop]

2017-09-27T12:41:50Z

Protected "5opy" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:41, 27 September 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5opy is ON HOLD until Paper Publication Authors: Backovic, M., Veesler, D., Borst, A.J., James, Z.M., Zagotta, W., Ginsberg, M., Rey, F.A., DiMaio, ...

2017-09-27T12:41:49Z

New page: '''Unreleased structure''' The entry 5opy is ON HOLD until Paper Publication Authors: Backovic, M., Veesler, D., Borst, A.J., James, Z.M., Zagotta, W., Ginsberg, M., Rey, F.A., DiMaio, ...

New page

'''Unreleased structure'''

The entry 5opy is ON HOLD until Paper Publication

Authors: Backovic, M., Veesler, D., Borst, A.J., James, Z.M., Zagotta, W., Ginsberg, M., Rey, F.A., DiMaio, F.

Description: Crystal structure of anti-alphaVbeta3 integrin Fab LM609
[[Category: Unreleased Structures]]
[[Category: Zagotta, W]]
[[Category: Backovic, M]]
[[Category: Veesler, D]]
[[Category: James, Z.M]]
[[Category: Ginsberg, M]]
[[Category: Borst, A.J]]
[[Category: Rey, F.A]]
[[Category: Dimaio, F]]