5tk7 - Revision history

OCA at 09:52, 25 December 2024

2024-12-25T09:52:47Z

@@ Line 10: / Line 10: @@
 == Function ==
 [https://www.uniprot.org/uniprot/O24769_PRIMG O24769_PRIMG]
 __TOC__
 </StructureSection>

OCA at 15:41, 6 March 2024

2024-03-06T15:41:33Z

← Older revision		Revision as of 15:41, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='5tk7' size='340' side='right'caption='[[5tk7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5tk7' size='340' side='right'caption='[[5tk7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5tk7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_14581 Atcc 14581~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TK7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TK7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5tk7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TK7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7D4:[[(2~{S},3~{R},4~{R})-4-(6-AMINOPURIN-9-YL)-3-(HYDROXYMETHYL)OXETAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+PHOSPHONO+HYDROGEN+PHOSPHATE'>7D4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.904Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tk6\|5tk6]], [[5tk8\|5tk8]], [[5tk9\|5tk9]], [[5tka\|5tka]]</td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7D4:[[(2~{S},3~{R},4~{R})-4-(6-AMINOPURIN-9-YL)-3-(HYDROXYMETHYL)OXETAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+PHOSPHONO+HYDROGEN+PHOSPHATE'>7D4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">oxsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tk7 OCA], [https://pdbe.org/5tk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tk7 RCSB], [https://www.ebi.ac.uk/pdbsum/5tk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tk7 ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tk7 OCA], [~~http~~://pdbe.org/5tk7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tk7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tk7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tk7 ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/O24769_PRIMG O24769_PRIMG]
	HD domain phosphohydrolase enzymes are characterized by a conserved set of histidine and aspartate residues that coordinate an active site metallocenter. Despite the important roles these enzymes play in nucleotide metabolism and signal transduction, few have been both biochemically and structurally characterized. Here, we present X-ray crystal structures and biochemical characterization of the Bacillus megaterium HD domain phosphohydrolase OxsA, involved in the biosynthesis of the antitumor, antiviral, and antibacterial compound oxetanocin-A. These studies reveal a previously uncharacterized reaction for this family; OxsA catalyzes the conversion of a triphosphorylated compound into a nucleoside, releasing one molecule of inorganic phosphate at a time. Remarkably, this functionality is a result of the OxsA active site, which based on structural and kinetic analyses has been tailored to bind the small, four-membered ring of oxetanocin-A over larger substrates. Furthermore, our OxsA structures show an active site that switches from a dinuclear to a mononuclear metal center as phosphates are eliminated from substrate.

	An HD domain phosphohydrolase active site tailored for oxetanocin-A biosynthesis.,Bridwell-Rabb J, Kang G, Zhong A, Liu HW, Drennan CL Proc Natl Acad Sci U S A. 2016 Nov 29;113(48):13750-13755. Epub 2016 Nov 14. PMID:27849620<ref>PMID:~~27849620<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 5tk7" style="background-color:#fffaf0;"><~~/~~div>~~
	~~== References ==~~
	~~<references~~/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 14581]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Bridwell-Rabb, J~~]]		[[Category: Priestia megaterium]]
	[[Category: ~~Drennan, C L~~]]		[[Category: Bridwell-Rabb J]]
	[[Category: ~~Metal binding protein]]~~		[[Category: Drennan CL]]
	~~[[Category: Phosphohydrolase~~]]

OCA at 12:33, 25 December 2019

2019-12-25T12:33:24Z

← Older revision		Revision as of 12:33, 25 December 2019
Line 1:		Line 1:

	==Structure of the HD-domain phosphohydrolase OxsA with Oxetanocin-A triphosphate bound==		==Structure of the HD-domain phosphohydrolase OxsA with Oxetanocin-A triphosphate bound==
	<StructureSection load='5tk7' size='340' side='right' caption='[[5tk7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5tk7' size='340' side='right'caption='[[5tk7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5tk7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TK7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5tk7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TK7 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 14581]]		[[Category: Atcc 14581]]
			[[Category: Large Structures]]
	[[Category: Bridwell-Rabb, J]]		[[Category: Bridwell-Rabb, J]]
	[[Category: Drennan, C L]]		[[Category: Drennan, C L]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Phosphohydrolase]]		[[Category: Phosphohydrolase]]

OCA at 14:25, 6 November 2017

2017-11-06T14:25:28Z

← Older revision		Revision as of 14:25, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5tk7' size='340' side='right' caption='[[5tk7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5tk7' size='340' side='right' caption='[[5tk7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5tk7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TK7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5tk7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TK7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7D4:[[(2~{S},3~{R},4~{R})-4-(6-AMINOPURIN-9-YL)-3-(HYDROXYMETHYL)OXETAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+PHOSPHONO+HYDROGEN+PHOSPHATE'>7D4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7D4:[[(2~{S},3~{R},4~{R})-4-(6-AMINOPURIN-9-YL)-3-(HYDROXYMETHYL)OXETAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+PHOSPHONO+HYDROGEN+PHOSPHATE'>7D4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tk6\|5tk6]], [[5tk8\|5tk8]], [[5tk9\|5tk9]], [[5tka\|5tka]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tk6\|5tk6]], [[5tk8\|5tk8]], [[5tk9\|5tk9]], [[5tka\|5tka]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">oxsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tk7 OCA], [http://pdbe.org/5tk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/5tk7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tk7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tk7 OCA], [http://pdbe.org/5tk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tk7 RCSB], [http://www.ebi.ac.uk/pdbsum/5tk7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tk7 ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 14581]]
	[[Category: Bridwell-Rabb, J]]		[[Category: Bridwell-Rabb, J]]
	[[Category: Drennan, C L]]		[[Category: Drennan, C L]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Phosphohydrolase]]		[[Category: Phosphohydrolase]]

OCA at 03:31, 10 December 2016

2016-12-10T03:31:37Z

@@ Line 1: / Line 1: @@
-The entry 5tk7 is ON HOLD
+==Structure of the HD-domain phosphohydrolase OxsA with Oxetanocin-A triphosphate bound==
-Authors:
+An HD domain phosphohydrolase active site tailored for oxetanocin-A biosynthesis.,Bridwell-Rabb J, Kang G, Zhong A, Liu HW, Drennan CL Proc Natl Acad Sci U S A. 2016 Nov 29;113(48):13750-13755. Epub 2016 Nov 14. PMID:27849620<ref>PMID:27849620</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>

OCA: Protected "5tk7" [edit=sysop:move=sysop]

2016-10-19T15:57:19Z

Protected "5tk7" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:57, 19 October 2016
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5tk7 is ON HOLD Authors: Description: Category: Unreleased Structures

2016-10-19T15:57:17Z

New page: '''Unreleased structure''' The entry 5tk7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5tk7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]