5ttp - Revision history

OCA at 05:53, 14 May 2025

2025-05-14T05:53:06Z

@@ Line 9: / Line 9: @@
 == Function ==
 [https://www.uniprot.org/uniprot/MSBA_ECOLI MSBA_ECOLI] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
 __TOC__
 </SX>

OCA at 15:43, 6 March 2024

2024-03-06T15:43:13Z

← Older revision		Revision as of 15:43, 6 March 2024
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	<SX load='5ttp' size='340' side='right' viewer='molstar' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>		<SX load='5ttp' size='340' side='right' viewer='molstar' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5TTP FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TTP FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~msbA~~, ~~Z1260, ECs0997 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5~~&~~id=83334 ECO57])~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.8Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ttp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ttp OCA], [~~http~~://pdbe.org/5ttp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ttp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ttp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ttp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ttp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ttp OCA], [https://pdbe.org/5ttp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ttp RCSB], [https://www.ebi.ac.uk/pdbsum/5ttp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ttp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/~~MSBA_ECO57 MSBA_ECO57]~~] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.		[https://www.uniprot.org/uniprot/MSBA_ECOLI MSBA_ECOLI] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria is critical for the assembly of their cell envelopes. LPS synthesized in the cytoplasmic leaflet of the inner membrane is flipped to the periplasmic leaflet by MsbA, an ATP-binding cassette transporter. Despite substantial efforts, the structural mechanisms underlying MsbA-driven LPS flipping remain elusive. Here we use single-particle cryo-electron microscopy to elucidate the structures of lipid-nanodisc-embedded MsbA in three functional states. The 4.2 A-resolution structure of the transmembrane domains of nucleotide-free MsbA reveals that LPS binds deep inside MsbA at the height of the periplasmic leaflet, establishing extensive hydrophilic and hydrophobic interactions with MsbA. Two sub-nanometre-resolution structures of MsbA with ADP-vanadate and ADP reveal an unprecedented closed and an inward-facing conformation, respectively. Our study uncovers the structural basis for LPS recognition, delineates the conformational transitions of MsbA to flip LPS, and paves the way for structural characterization of other lipid flippases.

	Structural basis of MsbA-mediated lipopolysaccharide transport.,Mi W, Li Y, Yoon SH, Ernst RK, Walz T, Liao M Nature. 2017 Sep 14;549(7671):233-237. doi: 10.1038/nature23649. Epub 2017 Sep 6. PMID:28869968<ref>PMID:28869968</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 5ttp" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: ~~Eco57~~]]		[[Category: Escherichia coli O157:H7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Liao, M]]		[[Category: Liao M]]
	[[Category: Mi, W]]		[[Category: Mi W]]
	[[Category: Walz, T]]		[[Category: Walz T]]
	~~[[Category: Abc transporter]]~~
	~~[[Category: Flippase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Lp]]~~
	~~[[Category: Nanodisc~~]]

OCA at 21:47, 10 April 2020

2020-04-10T21:47:37Z

← Older revision		Revision as of 21:47, 10 April 2020
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	<SX load='5ttp' size='340' side='right' viewer='molstar' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>		<SX load='5ttp' size='340' side='right' viewer='molstar' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TTP FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TTP FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">msbA, Z1260, ECs0997 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">msbA, Z1260, ECs0997 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ttp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ttp OCA], [http://pdbe.org/5ttp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ttp RCSB], [http://www.ebi.ac.uk/pdbsum/5ttp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ttp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ttp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ttp OCA], [http://pdbe.org/5ttp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ttp RCSB], [http://www.ebi.ac.uk/pdbsum/5ttp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ttp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 19:39, 6 March 2020

2020-03-06T19:39:48Z

← Older revision		Revision as of 19:39, 6 March 2020
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	==Cryo-EM structure of MsbA-nanodisc with ADP-vanadate==		==Cryo-EM structure of MsbA-nanodisc with ADP-vanadate==
	<~~StructureSection~~ load='5ttp' size='340' side='right'caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>		<SX load='5ttp' size='340' side='right' viewer='molstar' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TTP FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TTP FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
	</~~StructureSection~~>		</SX>
	[[Category: Eco57]]		[[Category: Eco57]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 07:22, 6 November 2019

2019-11-06T07:22:43Z

← Older revision		Revision as of 07:22, 6 November 2019
Line 1:		Line 1:

	==Cryo-EM structure of MsbA-nanodisc with ADP-vanadate==		==Cryo-EM structure of MsbA-nanodisc with ADP-vanadate==
	<StructureSection load='5ttp' size='340' side='right' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>		<StructureSection load='5ttp' size='340' side='right'caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TTP FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TTP FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Eco57]]		[[Category: Eco57]]
			[[Category: Large Structures]]
	[[Category: Liao, M]]		[[Category: Liao, M]]
	[[Category: Mi, W]]		[[Category: Mi, W]]

OCA at 14:27, 6 November 2017

2017-11-06T14:27:25Z

← Older revision		Revision as of 14:27, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5ttp' size='340' side='right' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>		<StructureSection load='5ttp' size='340' side='right' caption='[[5ttp]], [[Resolution\|resolution]] 4.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TTP FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ttp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TTP FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ttp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ttp OCA], [http://pdbe.org/5ttp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ttp RCSB], [http://www.ebi.ac.uk/pdbsum/5ttp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ttp ProSAT]</span></td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">msbA, Z1260, ECs0997 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ttp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ttp OCA], [http://pdbe.org/5ttp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ttp RCSB], [http://www.ebi.ac.uk/pdbsum/5ttp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ttp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Eco57]]
	[[Category: Liao, M]]		[[Category: Liao, M]]
	[[Category: Mi, W]]		[[Category: Mi, W]]

OCA at 04:35, 21 September 2017

2017-09-21T04:35:43Z

@@ Line 1: / Line 1: @@
-The entry 5ttp is ON HOLD  until Paper Publication
+==Cryo-EM structure of MsbA-nanodisc with ADP-vanadate==
-Authors:
+Structural basis of MsbA-mediated lipopolysaccharide transport.,Mi W, Li Y, Yoon SH, Ernst RK, Walz T, Liao M Nature. 2017 Sep 14;549(7671):233-237. doi: 10.1038/nature23649. Epub 2017 Sep 6. PMID:28869968<ref>PMID:28869968</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>

OCA: Protected "5ttp" [edit=sysop:move=sysop]

2016-12-09T17:51:57Z

Protected "5ttp" [edit=sysop:move=sysop]

← Older revision	Revision as of 17:51, 9 December 2016
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5ttp is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2016-12-09T17:51:56Z

New page: '''Unreleased structure''' The entry 5ttp is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5ttp is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]