5uce - Revision history

OCA at 10:25, 14 June 2023

2023-06-14T10:25:35Z

← Older revision		Revision as of 10:25, 14 June 2023
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	==Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)==		==Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)==
	<StructureSection load='5uce' size='340' side='right'caption='[[5uce~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>		<StructureSection load='5uce' size='340' side='right'caption='[[5uce]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uce]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Streptococcus_gx7809~~ Streptococcus ~~gx7809~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UCE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uce]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp._GX7805 Streptococcus sp. GX7805]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UCE FirstGlance]. <br>
	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ub0\|5ub0]], [[5ubs\|5ubs]], [[5ucf\|5ucf]]</td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uce OCA], [https://pdbe.org/5uce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uce RCSB], [https://www.ebi.ac.uk/pdbsum/5uce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uce ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1325 Streptococcus GX7809])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5uce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uce OCA], [~~http~~://pdbe.org/5uce PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5uce RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5uce PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5uce ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/SPG2_STRSG SPG2_STRSG]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5uce" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5uce" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Protein G\|Protein G]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Streptococcus ~~gx7809~~]]		[[Category: Streptococcus sp. GX7805]]
	[[Category: Chica~~, R A~~]]		[[Category: Chica RA]]
	[[Category: Damry~~, A M~~]]		[[Category: Damry AM]]
	[[Category: Davey~~, J A~~]]		[[Category: Davey JA]]
	[[Category: Goto~~, N K]]~~		[[Category: Goto NK]]
	~~[[Category: Computational design]]~~
	~~[[Category: Conformational exchange]]~~
	~~[[Category: De novo protein]]~~
	~~[[Category: Dynamic]]~~
	~~[[Category: Immunoglobulin-binding~~]]

OCA at 08:21, 8 January 2020

2020-01-08T08:21:03Z

← Older revision		Revision as of 08:21, 8 January 2020
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	==Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)==		==Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)==
	<StructureSection load='5uce' size='340' side='right' caption='[[5uce]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='5uce' size='340' side='right'caption='[[5uce]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uce]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gx7809 Streptococcus gx7809]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UCE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uce]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gx7809 Streptococcus gx7809]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UCE FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Streptococcus gx7809]]		[[Category: Streptococcus gx7809]]
	[[Category: Chica, R A]]		[[Category: Chica, R A]]

OCA at 07:31, 8 November 2017

2017-11-08T07:31:45Z

← Older revision		Revision as of 07:31, 8 November 2017
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	<StructureSection load='5uce' size='340' side='right' caption='[[5uce]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='5uce' size='340' side='right' caption='[[5uce]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uce]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UCE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uce]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gx7809 Streptococcus gx7809]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UCE FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ub0\|5ub0]], [[5ubs\|5ubs]], [[5ucf\|5ucf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ub0\|5ub0]], [[5ubs\|5ubs]], [[5ucf\|5ucf]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spg ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1325 Streptococcus GX7809])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uce OCA], [http://pdbe.org/5uce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uce RCSB], [http://www.ebi.ac.uk/pdbsum/5uce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uce ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uce OCA], [http://pdbe.org/5uce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uce RCSB], [http://www.ebi.ac.uk/pdbsum/5uce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uce ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Proteins are intrinsically dynamic molecules that can exchange between multiple conformational states, enabling them to carry out complex molecular processes with extreme precision and efficiency. Attempts to design novel proteins with tailored functions have mostly failed to yield efficiencies matching those found in nature because standard methods do not allow the design of exchange between necessary conformational states on a functionally relevant timescale. Here we developed a broadly applicable computational method to engineer protein dynamics that we term meta-multistate design. We used this methodology to design spontaneous exchange between two novel conformations introduced into the global fold of Streptococcal protein G domain beta1. The designed proteins, named DANCERs, for dynamic and native conformational exchangers, are stably folded and switch between predicted conformational states on the millisecond timescale. The successful introduction of defined dynamics on functional timescales opens the door to new applications requiring a protein to spontaneously access multiple conformational states.

			Rational design of proteins that exchange on functional timescales.,Davey JA, Damry AM, Goto NK, Chica RA Nat Chem Biol. 2017 Oct 23. doi: 10.1038/nchembio.2503. PMID:29058725<ref>PMID:29058725</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5uce" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Streptococcus gx7809]]
	[[Category: Chica, R A]]		[[Category: Chica, R A]]
	[[Category: Damry, A M]]		[[Category: Damry, A M]]

OCA at 10:09, 18 October 2017

2017-10-18T10:09:28Z

← Older revision		Revision as of 10:09, 18 October 2017
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry 5uce is ON HOLD until Paper Publication~~		==Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)==
			<StructureSection load='5uce' size='340' side='right' caption='[[5uce]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	~~Authors: Damry, A.M., Davey, J.A., Goto, N.K., Chica, R.A.~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[5uce]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UCE FirstGlance]. <br>
	~~Description:~~ Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ub0\|5ub0]], [[5ubs\|5ubs]], [[5ucf\|5ucf]]</td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uce OCA], [http://pdbe.org/5uce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uce RCSB], [http://www.ebi.ac.uk/pdbsum/5uce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uce ProSAT]</span></td></tr>
	[[Category: Chica, R.A]]		</table>
	[[Category: ~~Goto~~, ~~N.K~~]]		__TOC__
	[[Category: Davey, J.A]]		</StructureSection>
	[[Category: ~~Damry~~, ~~A.M~~]]		[[Category: Chica, R A]]
			[[Category: Damry, A M]]
			[[Category: Davey, J A]]
			[[Category: Goto, N K]]
			[[Category: Computational design]]
			[[Category: Conformational exchange]]
			[[Category: De novo protein]]
			[[Category: Dynamic]]
			[[Category: Immunoglobulin-binding]]

OCA at 18:55, 15 March 2017

2017-03-15T18:55:49Z

← Older revision		Revision as of 18:55, 15 March 2017
Line 7:		Line 7:
	Description: Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)		Description: Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	~~[[Category: Damry, A.M]]~~
	[[Category: Chica, R.A]]		[[Category: Chica, R.A]]
	[[Category: Goto, N.K]]		[[Category: Goto, N.K]]
	[[Category: Davey, J.A]]		[[Category: Davey, J.A]]
			[[Category: Damry, A.M]]

OCA at 14:21, 9 March 2017

2017-03-09T14:21:01Z

← Older revision		Revision as of 14:21, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5uce is ON HOLD		The entry 5uce is ON HOLD until Paper Publication

	Authors: Damry, A.M., Davey, J.A., Goto, N.K., Chica, R.A.		Authors: Damry, A.M., Davey, J.A., Goto, N.K., Chica, R.A.

OCA: Protected "5uce" [edit=sysop:move=sysop]

2017-01-04T15:39:24Z

Protected "5uce" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:39, 4 January 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5uce is ON HOLD Authors: Damry, A.M., Davey, J.A., Goto, N.K., Chica, R.A. Description: Solution NMR structure of the major species of DANCER-2, a ...

2017-01-04T15:39:23Z

New page: '''Unreleased structure''' The entry 5uce is ON HOLD Authors: Damry, A.M., Davey, J.A., Goto, N.K., Chica, R.A. Description: Solution NMR structure of the major species of DANCER-2, a ...

New page

'''Unreleased structure'''

The entry 5uce is ON HOLD

Authors: Damry, A.M., Davey, J.A., Goto, N.K., Chica, R.A.

Description: Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)
[[Category: Unreleased Structures]]
[[Category: Damry, A.M]]
[[Category: Chica, R.A]]
[[Category: Goto, N.K]]
[[Category: Davey, J.A]]